FastQCFastQC Report
Fri 27 May 2016
SRR939495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598085
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114330.7154187668365575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108470.6787498787611422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.43877515901845027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52010.32545202539289214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.19792439075518511No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30850.19304354899770665No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29900.18709893403667516No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27200.17020371256848038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21300.13328452491575854No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.10137132880916847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105200.031.8466171
TACCTGG15200.030.2356852
GTACCTG17750.027.4978351
GTATCAA148400.026.8228551
GTACATG121600.025.4471551
TACATGG121900.025.341692
ACCTGGG17300.024.9214533
CGTTACG601.727455E-423.71801834-35
ACATGGG122050.023.6793693
CATGGGT16200.023.3966184
ATAACGC1050.002229627522.5610243
TATCAAC180400.022.0352062
GAGTACT103700.021.88440912-13
ATCAACG182050.021.6526363
TAGGACC10350.021.5147134
TCAACGC184250.021.368384
CGTACAC1353.8632652E-421.0569533
CAACGCA187500.020.9979955
AACGCAG192450.020.5317636
CATGGGG44150.019.9599914
Produced by FastQC (version 0.11.5)