##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1598085 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.025987353613857 31.0 31.0 33.0 30.0 34.0 2 31.269322970930833 31.0 31.0 34.0 30.0 34.0 3 31.254503984456395 31.0 31.0 34.0 28.0 34.0 4 35.18669657746616 35.0 35.0 37.0 33.0 37.0 5 34.82743533666858 35.0 35.0 37.0 32.0 37.0 6 34.80652092973778 35.0 35.0 37.0 32.0 37.0 7 34.69362643413836 35.0 35.0 37.0 31.0 37.0 8 34.71513905705892 35.0 35.0 37.0 31.0 37.0 9 36.04957621152817 37.0 35.0 39.0 30.0 39.0 10-11 35.89998654639772 37.0 35.0 39.0 30.0 39.0 12-13 35.87524724905121 37.0 35.0 39.0 30.0 39.0 14-15 36.69516640228774 38.0 36.0 40.0 31.0 41.0 16-17 36.72224005606711 38.0 36.0 40.0 31.0 41.0 18-19 36.750619335016594 38.5 35.5 40.0 31.0 41.0 20-21 36.67236348504616 38.5 35.0 40.0 30.0 41.0 22-23 36.5294380461615 38.0 35.0 40.0 30.0 41.0 24-25 36.320703529536914 38.0 34.5 40.0 29.5 41.0 26-27 35.943368782010964 38.0 34.0 40.0 28.5 41.0 28-29 35.65814584330621 38.0 34.0 40.0 27.0 41.0 30-31 35.414288038496075 38.0 34.0 40.0 27.0 41.0 32-33 35.16439707524944 37.5 34.0 40.0 27.0 41.0 34-35 35.06300415810173 38.0 33.0 40.0 26.5 41.0 36-37 34.99326193537891 38.0 33.5 40.0 25.5 41.0 38-39 34.94953459922344 38.0 33.0 40.0 26.0 41.0 40-41 34.6878795558434 38.0 33.0 40.0 25.0 41.0 42-43 34.37241667370634 38.0 33.0 40.0 24.0 41.0 44-45 34.147442720506106 38.0 33.0 40.0 24.0 41.0 46-47 33.723219353163316 37.0 33.0 40.0 22.5 41.0 48-49 33.361335911418976 37.0 32.5 40.0 20.0 41.0 50-51 33.099623612010625 37.0 32.0 40.0 18.5 41.0 52-53 32.73658316047019 37.0 31.0 40.0 15.5 41.0 54-55 32.56086440959023 37.0 31.0 40.0 15.0 41.0 56-57 32.200174896829644 36.0 31.0 39.0 13.0 40.5 58-59 31.814003009852417 36.0 30.0 39.0 10.5 40.0 60-61 31.58243147266885 35.5 30.0 39.0 8.5 40.0 62-63 31.21692995053455 35.0 29.0 39.0 8.0 40.0 64-65 30.816026994809413 35.0 29.0 38.5 7.5 40.0 66-67 29.23556976005657 34.0 25.5 37.5 4.0 40.0 68-69 25.743776144573033 30.5 16.0 36.0 2.0 39.0 70-71 25.18356313963275 29.5 15.0 35.0 2.0 39.0 72-73 26.917374545158737 31.0 21.5 35.5 2.0 38.5 74-75 27.49429097951611 32.0 24.5 35.5 2.0 38.0 76-77 27.440302299314492 31.5 25.5 35.0 2.0 37.0 78-79 28.270539739751015 33.0 26.0 35.0 2.0 37.5 80-81 28.12151669028869 33.0 26.0 35.0 2.0 37.0 82-83 27.728521323959615 33.0 26.0 35.0 2.0 37.0 84-85 27.390467340598278 33.0 26.0 35.0 2.0 36.0 86-87 26.918015937825587 32.5 25.5 35.0 2.0 36.0 88-89 26.418109174418134 32.0 24.5 35.0 2.0 35.0 90-91 25.969448120719484 32.0 24.0 34.0 2.0 35.0 92-93 25.46286430321291 32.0 21.5 34.0 2.0 35.0 94-95 24.17451731290889 31.0 13.0 34.0 2.0 35.0 96-97 23.349494238416604 30.5 4.5 34.0 2.0 35.0 98-99 23.027680943128807 31.0 2.0 34.0 2.0 35.0 100-101 21.814585269244127 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3709792773833982 1101 2 0.2899311085194185 1101 3 0.30409764010359197 1101 4 0.24824111713412833 1101 5 0.4151938369798387 1101 6 0.5896801178954334 1101 7 0.4646919880506175 1101 8 0.5261025545827351 1101 9 0.5256919679449865 1101 10-11 0.620203789458067 1101 12-13 0.4210996388913344 1101 14-15 0.5366811297712673 1101 16-17 0.4186872101346424 1101 18-19 0.6548148155894822 1101 20-21 0.6346197086026066 1101 22-23 0.7362521987872768 1101 24-25 0.549678155199743 1101 26-27 0.65480551771671 1101 28-29 0.65109946786189 1101 30-31 0.4607984372551499 1101 32-33 0.5303034002837279 1101 34-35 0.7841480556111193 1101 36-37 0.7804287508491612 1101 38-39 0.6737504762452602 1101 40-41 0.9196437904669992 1101 42-43 0.7022347901688377 1101 44-45 0.549642533092964 1101 46-47 0.7115808529770646 1101 48-49 0.4691263150697438 1101 50-51 0.5092439271073985 1101 52-53 0.7618176442224254 1101 54-55 0.3092022100220646 1101 56-57 0.5291901870979174 1101 58-59 0.49889725729201473 1101 60-61 0.27811614551454156 1101 62-63 0.42085568173712673 1101 64-65 0.28640999205752493 1101 66-67 -2.8746420357484297 1101 68-69 -7.795690292475879 1101 70-71 -11.499085580740541 1101 72-73 -11.525178014802673 1101 74-75 -9.750182980315671 1101 76-77 -4.513604884974143 1101 78-79 -1.516755862999041 1101 80-81 -0.7508340850422677 1101 82-83 -0.6261240370396486 1101 84-85 -0.754570347001426 1101 86-87 -0.564271096725161 1101 88-89 -0.34181527143999446 1101 90-91 -0.5749630526651082 1101 92-93 -1.2419335723989668 1101 94-95 -4.6682929725246645 1101 96-97 -1.4774463893209067 1101 98-99 -1.1652232027051603 1101 100-101 -0.996938547070954 1102 1 0.429714366387163 1102 2 0.3740986441468088 1102 3 0.4338589902097141 1102 4 0.34403349838232344 1102 5 0.4788864393508021 1102 6 0.5949460198766658 1102 7 0.6263112871136229 1102 8 0.5673012766558188 1102 9 0.8317425229705364 1102 10-11 0.7734137673457866 1102 12-13 0.8200337753662339 1102 14-15 0.7374746573080841 1102 16-17 0.8374685944075608 1102 18-19 0.7387996845797886 1102 20-21 0.9347803738937301 1102 22-23 0.9005711924198323 1102 24-25 0.9428150294555806 1102 26-27 0.9110226543617088 1102 28-29 0.962806032767709 1102 30-31 0.8834038033649421 1102 32-33 0.6520958259043894 1102 34-35 0.8930842997779891 1102 36-37 1.097631769655699 1102 38-39 1.1524400464979578 1102 40-41 0.8445452466233121 1102 42-43 0.9861246281353928 1102 44-45 0.6951459386794525 1102 46-47 0.7745753286054864 1102 48-49 0.7364337940482883 1102 50-51 0.6250116816389308 1102 52-53 0.7633936061804363 1102 54-55 0.7251304449594613 1102 56-57 0.6625268483659355 1102 58-59 0.5024106318917418 1102 60-61 0.19282894475133716 1102 62-63 0.42294192701330857 1102 64-65 0.35234968988105564 1102 66-67 -2.5353359840840923 1102 68-69 -7.9200100996035765 1102 70-71 -11.400440974583121 1102 72-73 -9.4573284522412 1102 74-75 -7.678891039094392 1102 76-77 -3.915656186936147 1102 78-79 -1.110200940983642 1102 80-81 -0.5707869023238636 1102 82-83 -0.31337231575787783 1102 84-85 -0.5597794892077701 1102 86-87 -0.551970865248812 1102 88-89 -0.20898612021124663 1102 90-91 -0.5936982156229753 1102 92-93 -0.9639214217396663 1102 94-95 -4.65247455943183 1102 96-97 -1.5117085862015216 1102 98-99 -0.7040272908880354 1102 100-101 -0.37249819475360013 1103 1 0.3734342215097435 1103 2 0.25810206587478035 1103 3 0.3710994030011534 1103 4 0.16802563568825946 1103 5 0.5392253360255381 1103 6 0.5047834877979653 1103 7 0.4062832580653364 1103 8 0.40458719114901953 1103 9 0.5632861227011645 1103 10-11 0.6278629237442388 1103 12-13 0.4312271940000869 1103 14-15 0.7683812332140221 1103 16-17 0.7302320037590704 1103 18-19 0.6012416699100456 1103 20-21 0.5806506394416644 1103 22-23 0.5892003171490998 1103 24-25 0.6706147491650967 1103 26-27 0.638582125085911 1103 28-29 0.6212729894318869 1103 30-31 0.7529131130131219 1103 32-33 0.6111788756104488 1103 34-35 0.6456469556213165 1103 36-37 0.6707120528582848 1103 38-39 0.7192911467291125 1103 40-41 0.6418206047217083 1103 42-43 0.6579044727015813 1103 44-45 0.4943614893410526 1103 46-47 0.5928824832930744 1103 48-49 0.6387316299873547 1103 50-51 0.5235474368761786 1103 52-53 0.728526562152986 1103 54-55 0.7291127538978159 1103 56-57 0.6599141406506632 1103 58-59 0.42750027024421655 1103 60-61 0.5959403511179353 1103 62-63 0.5048249694390954 1103 64-65 0.3479314746398039 1103 66-67 -2.8703786646803486 1103 68-69 -7.799976610351077 1103 70-71 -11.511343182325692 1103 72-73 -9.277318259860003 1103 74-75 -7.458472605444975 1103 76-77 -3.802966672348301 1103 78-79 -1.077621722520874 1103 80-81 -0.4998652198529392 1103 82-83 -0.032476660260361 1103 84-85 -0.21881885728014083 1103 86-87 -0.18218672532198354 1103 88-89 1.624519453535811E-4 1103 90-91 -0.1344258788375825 1103 92-93 -0.08320322287903181 1103 94-95 0.9586851879427236 1103 96-97 1.1375870474551775 1103 98-99 0.7272014370663911 1103 100-101 0.6828901167692507 1104 1 0.4190273711247485 1104 2 0.3119357921090291 1104 3 0.313037325845162 1104 4 0.19359332104416893 1104 5 0.4003645228098023 1104 6 0.5153408126955199 1104 7 0.524984509523108 1104 8 0.40699381328295203 1104 9 0.6518746395328989 1104 10-11 0.6821838233387396 1104 12-13 0.6090882675646938 1104 14-15 0.8849165960797194 1104 16-17 0.8301453396840017 1104 18-19 0.8608474731932958 1104 20-21 0.8179135169029408 1104 22-23 0.6136590233222208 1104 24-25 0.7997410112329817 1104 26-27 1.0688624815855405 1104 28-29 0.9628954621755028 1104 30-31 0.8193475461123825 1104 32-33 0.6563897046967639 1104 34-35 0.7924888881945265 1104 36-37 0.7363333415495177 1104 38-39 0.5799756146009898 1104 40-41 0.7696743671194852 1104 42-43 1.0723702403919262 1104 44-45 0.6868424000627869 1104 46-47 0.4521448400566186 1104 48-49 0.2941579423417977 1104 50-51 0.17292973243990417 1104 52-53 0.4677354582381241 1104 54-55 0.4673726248646446 1104 56-57 0.387652529269495 1104 58-59 0.43801729456365024 1104 60-61 0.3815047197006045 1104 62-63 0.4822705547425805 1104 64-65 0.38556312022296524 1104 66-67 -2.5937927656029807 1104 68-69 -6.989384691151955 1104 70-71 -8.442823996754012 1104 72-73 -7.701807815590712 1104 74-75 -6.657488807703945 1104 76-77 -3.51753800615478 1104 78-79 -0.8991860982363846 1104 80-81 -0.27095526133043535 1104 82-83 -0.04561161726713436 1104 84-85 0.0021798837725981457 1104 86-87 0.2348270731679989 1104 88-89 0.13176327650231556 1104 90-91 -0.13075638637521436 1104 92-93 0.21074634076913057 1104 94-95 1.0266118123591674 1104 96-97 1.1383094264018716 1104 98-99 0.8769772873907584 1104 100-101 0.8063093890050084 1105 1 0.41902215882065263 1105 2 0.3196228981891487 1105 3 0.30294213527290026 1105 4 0.21235587835345626 1105 5 0.292208517853247 1105 6 0.45453928542092115 1105 7 0.459127394762767 1105 8 0.5074519400642572 1105 9 0.6772213793756805 1105 10-11 0.8647364345988393 1105 12-13 0.7538101494427636 1105 14-15 0.9225473467279528 1105 16-17 0.9485450858795446 1105 18-19 0.8379289720851588 1105 20-21 0.9129164462178423 1105 22-23 0.8102499332028685 1105 24-25 0.9223217202870302 1105 26-27 1.0786114014223855 1105 28-29 1.0848864858928735 1105 30-31 1.0556105186685514 1105 32-33 1.0737093572376253 1105 34-35 1.2263933973935437 1105 36-37 1.1176093900392274 1105 38-39 1.0734611031989019 1105 40-41 1.1660864254722654 1105 42-43 1.1304231459734737 1105 44-45 0.8805743611470263 1105 46-47 0.7852762255634929 1105 48-49 0.8299525720006429 1105 50-51 1.0131277860960566 1105 52-53 1.2807628131049924 1105 54-55 1.037111342484934 1105 56-57 1.2072628504655754 1105 58-59 1.3927508659023715 1105 60-61 1.3339381012419587 1105 62-63 1.229625310414086 1105 64-65 1.2099550354528326 1105 66-67 1.8415725786905526 1105 68-69 -4.749847696649891 1105 70-71 -5.12485781001314 1105 72-73 -1.4313173794737537 1105 74-75 1.2003812155012312 1105 76-77 1.214103737590282 1105 78-79 1.1631153564134422 1105 80-81 1.2047861541784606 1105 82-83 1.2113092801109353 1105 84-85 1.2201480164403335 1105 86-87 1.3466808603934055 1105 88-89 1.379466387830874 1105 90-91 1.3679459236484597 1105 92-93 1.3465140054414562 1105 94-95 2.1126231696223066 1105 96-97 2.272674981272491 1105 98-99 2.0332652720568554 1105 100-101 1.8585495330591897 1106 1 0.015401146765761098 1106 2 0.0021136989659851224 1106 3 0.05647847303977116 1106 4 -0.019225292009608097 1106 5 0.02801270973173331 1106 6 0.1043225539954662 1106 7 0.14000824794810995 1106 8 0.05401411050618066 1106 9 0.16900389677500982 1106 10-11 0.19815464271179906 1106 12-13 0.18636811490741678 1106 14-15 0.27273266780188976 1106 16-17 0.17002081102426558 1106 18-19 0.17647642377001915 1106 20-21 0.21649715946004022 1106 22-23 0.2789692329257889 1106 24-25 0.27358201051868036 1106 26-27 0.43554627239267774 1106 28-29 0.4639542934904384 1106 30-31 0.550978494278084 1106 32-33 0.5168733286288116 1106 34-35 0.6177175909828989 1106 36-37 0.4992114115292594 1106 38-39 0.3351611249856603 1106 40-41 0.48706379531646604 1106 42-43 0.4312587949848492 1106 44-45 0.40675388667695955 1106 46-47 0.5173328951840688 1106 48-49 0.3586004833459313 1106 50-51 0.30687939838271916 1106 52-53 0.47139992196257907 1106 54-55 0.4633250852488615 1106 56-57 0.4968639523674412 1106 58-59 0.3706092413055302 1106 60-61 0.4317018178751404 1106 62-63 0.5365779583067223 1106 64-65 0.5335588453279811 1106 66-67 1.2268255523152156 1106 68-69 -5.31265503357336 1106 70-71 -5.9440578217753135 1106 72-73 -2.2270805908680913 1106 74-75 0.40593088996969584 1106 76-77 0.4043859763062798 1106 78-79 0.2426086071976954 1106 80-81 0.19682730574877638 1106 82-83 0.3255433821290872 1106 84-85 0.3364709068342435 1106 86-87 0.4425191893092375 1106 88-89 0.40784296720349644 1106 90-91 0.2674922779759399 1106 92-93 0.41050557944758737 1106 94-95 1.233008019941952 1106 96-97 1.178708241493215 1106 98-99 0.9751330652190227 1106 100-101 0.9262268110782266 1107 1 -0.04592984755492324 1107 2 -0.06158234153417652 1107 3 -0.03918073602149974 1107 4 -0.10977191606139058 1107 5 0.14115643885484985 1107 6 0.2649093898400352 1107 7 9.331191822639084E-4 1107 8 0.022942089743438032 1107 9 0.1280391341607654 1107 10-11 0.14799503713982176 1107 12-13 0.19106416341789156 1107 14-15 0.06202227281314521 1107 16-17 -0.006448481424406793 1107 18-19 0.3944231391394126 1107 20-21 0.1623173758988159 1107 22-23 0.25690581524381884 1107 24-25 0.39037243658745524 1107 26-27 0.4219137136570268 1107 28-29 0.4046108591470059 1107 30-31 0.35087976711750457 1107 32-33 0.5376430800686691 1107 34-35 0.3691764700190916 1107 36-37 0.18720354539768636 1107 38-39 0.3109839848291358 1107 40-41 0.23131539137218482 1107 42-43 0.24210216124058093 1107 44-45 0.22937642450568774 1107 46-47 0.15829745754038527 1107 48-49 0.3361996310481672 1107 50-51 0.3026779869648166 1107 52-53 0.3356270464989777 1107 54-55 0.18480835570120036 1107 56-57 0.4565760615640855 1107 58-59 0.3072446276831826 1107 60-61 0.08556599493519457 1107 62-63 0.36642379975049977 1107 64-65 0.592877247429886 1107 66-67 1.330141623414793 1107 68-69 -5.182891898655587 1107 70-71 -5.6905382886166045 1107 72-73 -2.0426009811307466 1107 74-75 0.3091512377776695 1107 76-77 0.29265606818461976 1107 78-79 0.2859043721429444 1107 80-81 0.29770223265463613 1107 82-83 0.2808187768374708 1107 84-85 0.3783138004812656 1107 86-87 0.478176115430081 1107 88-89 0.41446950218054823 1107 90-91 0.2688640448116253 1107 92-93 0.27977920387585087 1107 94-95 1.1067844439761103 1107 96-97 1.375691956518171 1107 98-99 1.1398722492881 1107 100-101 1.0023128098446392 1108 1 0.12203718828775934 1108 2 0.047196760768240864 1108 3 0.08045926703819006 1108 4 0.02647798832322934 1108 5 0.12447004522545058 1108 6 0.2791470232486546 1108 7 0.2202063617715666 1108 8 0.0389330072887617 1108 9 0.06751359176993788 1108 10-11 0.2971343980667527 1108 12-13 0.26850255691846314 1108 14-15 0.3947109571857652 1108 16-17 0.28441551928414555 1108 18-19 0.3119392729767938 1108 20-21 0.3669624717356257 1108 22-23 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60-61 0.49482751654611334 2311 62-63 0.6686930164775475 2311 64-65 0.6551044780306121 2311 66-67 1.8747768075078035 2311 68-69 4.324733006559679 2311 70-71 4.540568656646965 2311 72-73 2.681027097617811 2311 74-75 1.8807832202530292 2311 76-77 1.0213407369295275 2311 78-79 0.5549462740161744 2311 80-81 0.4584079927579836 2311 82-83 0.44352976063279215 2311 84-85 0.35139072355818257 2311 86-87 0.6512361878877435 2311 88-89 0.8256927167170147 2311 90-91 0.7599107491113415 2311 92-93 0.7115482380545224 2311 94-95 1.2813098182002811 2311 96-97 1.53262528423463 2311 98-99 1.1987384834555392 2311 100-101 1.0914771595511574 2312 1 -0.20493978219128195 2312 2 -0.14665197956509246 2312 3 -0.16255129105492472 2312 4 -0.11558481993807135 2312 5 -0.07623076771231752 2312 6 0.10022858848497407 2312 7 0.10824152831299472 2312 8 0.15828889934993384 2312 9 -1.2512133521532292 2312 10-11 -1.1432322435521485 2312 12-13 -2.298077294971854 2312 14-15 -2.1951718428661664 2312 16-17 -0.18783391451827214 2312 18-19 -1.966081769770959 2312 20-21 -1.9590965177246389 2312 22-23 -0.8211900638201755 2312 24-25 -1.3696245899243777 2312 26-27 -1.5350510186493977 2312 28-29 -0.29205684293071954 2312 30-31 0.5372862801185434 2312 32-33 0.23913208081734894 2312 34-35 0.6384079029748975 2312 36-37 0.5239656822737189 2312 38-39 0.40738739159700543 2312 40-41 0.7101777401092448 2312 42-43 0.7518914418157436 2312 44-45 0.8313703872627443 2312 46-47 1.087683087096849 2312 48-49 1.1929664405484672 2312 50-51 1.0618546493305487 2312 52-53 1.2016554751697797 2312 54-55 1.1584531619200433 2312 56-57 1.1827284209738522 2312 58-59 1.2419520045887396 2312 60-61 1.3667756736391858 2312 62-63 0.9810511048675536 2312 64-65 1.3073268069819513 2312 66-67 2.5583004236123124 2312 68-69 4.546746344650078 2312 70-71 5.525657531508596 2312 72-73 3.542553613704939 2312 74-75 2.5892213005926124 2312 76-77 1.4290117398478088 2312 78-79 1.484769667819176 2312 80-81 1.5676319043496818 2312 82-83 1.7938895139970477 2312 84-85 1.5871728040441369 2312 86-87 1.775911883837832 2312 88-89 1.67200941860429 2312 90-91 1.7693233425374366 2312 92-93 1.761934292522966 2312 94-95 2.7132850997364457 2312 96-97 2.824489552240596 2312 98-99 2.682385829102362 2312 100-101 2.3444949253752725 2313 1 -0.24819593589696254 2313 2 -0.16656842436836072 2313 3 -0.08798917090588532 2313 4 -0.08890354392719502 2313 5 0.0031674939038737193 2313 6 0.03900870824315206 2313 7 -3.7292263135088177 2313 8 -1.1232373285878765 2313 9 -0.8361942414650159 2313 10-11 -0.20240649522373388 2313 12-13 -1.4219083071495078 2313 14-15 -0.39144005248462577 2313 16-17 -0.050562946424953736 2313 18-19 0.15802557046337284 2313 20-21 0.14961734737600096 2313 22-23 -1.372857002810143 2313 24-25 -2.4328500511919913 2313 26-27 -2.2667388784765805 2313 28-29 -0.5539781959610437 2313 30-31 -0.2447245231070383 2313 32-33 -2.281494006473487 2313 34-35 -2.537178953741389 2313 36-37 -1.2458514446193334 2313 38-39 -0.6294451359797364 2313 40-41 -0.5038883992113554 2313 42-43 -0.5915274651105591 2313 44-45 -0.2600541519502073 2313 46-47 -0.4107486510626899 2313 48-49 -0.41414952752884204 2313 50-51 -0.3161613312415952 2313 52-53 0.014964235329841813 2313 54-55 0.10221759905981287 2313 56-57 0.2368446677820586 2313 58-59 0.02664038663590418 2313 60-61 0.4736385412355908 2313 62-63 0.18254631998694748 2313 64-65 0.15911164594276883 2313 66-67 1.3724205470228306 2313 68-69 4.322699556302954 2313 70-71 4.423893517270894 2313 72-73 2.4512764494555412 2313 74-75 1.8513613132155982 2313 76-77 0.9657305323983216 2313 78-79 0.7119417665347711 2313 80-81 0.7351499818112437 2313 82-83 0.57900880762244 2313 84-85 0.6981910048521378 2313 86-87 0.7968951952251118 2313 88-89 0.9110836475288657 2313 90-91 0.7962705342434298 2313 92-93 0.6193482143309375 2313 94-95 1.1219839069603843 2313 96-97 1.2171469150617362 2313 98-99 1.018929373598393 2313 100-101 0.7179895381795944 2314 1 -0.34657922279809483 2314 2 -0.2182837150006911 2314 3 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-1.6620418876885985 2314 64-65 -0.6050470586923495 2314 66-67 -0.35244997258153177 2314 68-69 1.4279167904396566 2314 70-71 1.6819267033092409 2314 72-73 -0.36981155981843017 2314 74-75 0.045001244491786 2314 76-77 -0.14205865087278724 2314 78-79 0.09991655395424104 2314 80-81 0.37102077772354747 2314 82-83 0.18280606822653667 2314 84-85 0.19522381070737538 2314 86-87 -0.0659885456105016 2314 88-89 0.07831279822472581 2314 90-91 0.5650734975941951 2314 92-93 0.5060749416191506 2314 94-95 0.34265683106710654 2314 96-97 0.5327316179891461 2314 98-99 0.31445941741293737 2314 100-101 0.38395504746915066 2315 1 -0.4477739949686921 2315 2 -0.30004240467778587 2315 3 -0.3625320682335911 2315 4 -0.1572579743069369 2315 5 -1.0923318028612456 2315 6 -0.48461998373996806 2315 7 -0.21248932335410586 2315 8 -0.17288571114765006 2315 9 0.043833887930198046 2315 10-11 -1.789888914351721 2315 12-13 -1.3858323577824194 2315 14-15 -0.33391543926380507 2315 16-17 -1.9720116102786776 2315 18-19 -3.3651811805914846 2315 20-21 -2.3580479128209006 2315 22-23 -1.8602690984500825 2315 24-25 -1.3532438627250443 2315 26-27 -0.6490173594892426 2315 28-29 -0.6105886038372716 2315 30-31 -3.104851105385528 2315 32-33 -2.4498484368532374 2315 34-35 -1.4283885177357618 2315 36-37 -4.602039911567296 2315 38-39 -2.130851605740631 2315 40-41 -1.5398237016023515 2315 42-43 -1.6129058054762453 2315 44-45 -1.428056964889727 2315 46-47 -1.4734772023988398 2315 48-49 -4.395724773661044 2315 50-51 -3.707230248259876 2315 52-53 -2.3634464538403392 2315 54-55 -3.6339989972833635 2315 56-57 -6.251833250642697 2315 58-59 -6.540588867935121 2315 60-61 -5.568274257639235 2315 62-63 -6.489042990842872 2315 64-65 -5.877808185280859 2315 66-67 -4.033908566901221 2315 68-69 -1.5861612031907164 2315 70-71 0.9130637001119659 2315 72-73 -0.9544197530761025 2315 74-75 -2.8259945236339092 2315 76-77 -5.098545135674815 2315 78-79 -3.803951341763401 2315 80-81 -3.650011762211264 2315 82-83 -7.191736621350838 2315 84-85 -4.8544109642055275 2315 86-87 -5.1114029763642 2315 88-89 -3.406637871458475 2315 90-91 -3.0352343884007347 2315 92-93 -3.8153212652752515 2315 94-95 -1.9191553335459481 2315 96-97 -0.8096463338834106 2315 98-99 -2.634235183363632 2315 100-101 -2.0731497023267345 2316 1 -0.328375700313277 2316 2 -0.29787556220239964 2316 3 -0.9841411575444212 2316 4 -0.18718061847008727 2316 5 -0.3582183183324119 2316 6 -2.5716958452026972 2316 7 -0.4979499422007976 2316 8 -0.30963852408716974 2316 9 -0.033297792801164405 2316 10-11 -0.1992089741266838 2316 12-13 -0.5101972487810116 2316 14-15 -0.8919070443974206 2316 16-17 -1.3543384386893678 2316 18-19 -0.29684652869275396 2316 20-21 -4.238672693060003 2316 22-23 -3.820114386776389 2316 24-25 -2.6798338328375593 2316 26-27 -2.2325880345947198 2316 28-29 -1.3455075120651188 2316 30-31 -0.16110000658102308 2316 32-33 -1.809524509286426 2316 34-35 -2.7062684843321065 2316 36-37 -4.631108784633398 2316 38-39 -2.16272243028353 2316 40-41 -1.8249261101818206 2316 42-43 -3.180926199287775 2316 44-45 -0.734842082681574 2316 46-47 -0.842194238263815 2316 48-49 -0.3517374318888997 2316 50-51 0.0023216592647372636 2316 52-53 -2.4352214626307784 2316 54-55 -0.6589902068473599 2316 56-57 0.18961255631932517 2316 58-59 0.25452694605925075 2316 60-61 -0.8283568877376872 2316 62-63 -0.4619409778330237 2316 64-65 0.4594698562099957 2316 66-67 1.3241775354194552 2316 68-69 3.9109005935744072 2316 70-71 4.13283668710212 2316 72-73 2.286571148822631 2316 74-75 1.1648172731312059 2316 76-77 0.10896519308749575 2316 78-79 0.506108213440541 2316 80-81 -0.09188170771055582 2316 82-83 0.09872136036505452 2316 84-85 0.4459219735581854 2316 86-87 0.08974857075253695 2316 88-89 0.3405239165162115 2316 90-91 0.6013536038636822 2316 92-93 0.6609709288175729 2316 94-95 1.1246979540771171 2316 96-97 1.3509098809548412 2316 98-99 0.4148121531342568 2316 100-101 0.25214587608395433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 15.0 4 44.0 5 147.0 6 364.0 7 905.0 8 1701.0 9 2770.0 10 4630.0 11 7221.0 12 9618.0 13 11783.0 14 13947.0 15 17826.0 16 21568.0 17 24447.0 18 21239.0 19 15871.0 20 14872.0 21 14808.0 22 15907.0 23 18375.0 24 22369.0 25 27271.0 26 34908.0 27 43634.0 28 53120.0 29 64080.0 30 76062.0 31 88757.0 32 103482.0 33 120478.0 34 142643.0 35 167205.0 36 181570.0 37 167066.0 38 81317.0 39 6055.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.433659364299075 21.009563116002305 15.61218275509623 20.944594764602385 2 15.162206108217516 22.361874148037717 41.1397800200528 21.336139723691968 3 19.020828053576626 28.1372392582372 31.05085148787455 21.791081200311623 4 12.39483506822228 18.854316259773416 37.85687244420666 30.893976227797644 5 11.834922319477574 39.63214699602774 34.23311916019433 14.299811524300349 6 28.060522437792734 37.78028077355084 19.121573633442527 15.037623155213897 7 26.072267745457843 34.934061705103296 21.88068844898738 17.112982100451475 8 25.30947978361602 36.07624125124759 20.39828920238911 18.215989762747288 9 26.51429679898128 17.415969738781104 21.77568777630727 34.29404568593035 10-11 23.799078271806568 27.89329103270477 28.13561231098471 20.172018384503954 12-13 26.117071369795724 25.895368519196417 28.55930692047044 19.428253190537426 14-15 22.146913336900102 27.95489601616935 27.838631862510443 22.0595587844201 16-17 19.5958145985945 30.457452496857947 29.580305347953807 20.36642755659375 18-19 19.674656232371245 27.854368740666434 33.15353238466432 19.317442642298005 20-21 21.17879248562889 27.037963261642297 32.437709019767105 19.345535232961712 22-23 20.429858236576905 27.97548315640282 32.224193331393515 19.370465275626767 24-25 20.280366814030543 27.605352656460703 33.077902614691965 19.036377914816796 26-27 19.760768873609425 27.91809911656377 32.94263484010167 19.378497169725133 28-29 19.393922704319284 27.95973462252304 32.776358986218234 19.869983686939445 30-31 19.70287999274096 27.708130280990545 32.986359575909376 19.60263015035912 32-33 19.390810370210442 28.160493885105375 32.91592156789121 19.53277417679297 34-35 19.908393354106185 28.082556124559506 32.69405395392383 19.31499656741049 36-37 19.847086306072296 27.761386238769724 32.66219715800883 19.729330297149144 38-39 20.121580166334947 28.006627089952723 32.47408748322479 19.39770526048754 40-41 20.538374601869926 27.501704034301206 32.77694316293825 19.182978200890606 42-43 19.694829578730776 28.400078923384807 32.36989386057495 19.535197637309466 44-45 19.655466795610078 28.76270069636958 31.580341808606722 20.001490699413615 46-47 20.35222297140921 28.149495269630588 31.04631217838765 20.451969580572555 48-49 20.305975954871226 28.493020348287313 30.73301689071956 20.4679868061219 50-51 20.176038583799187 29.20593163276491 30.541926997698127 20.076102785737774 52-53 20.231767509840928 29.05569659252728 30.56417989331844 20.148356004313353 54-55 19.945212512835926 28.72971297618153 30.761796528664814 20.56327798231773 56-57 20.409838066640003 29.151917140998897 29.708032943789362 20.730211848571745 58-59 20.3987148848738 28.980366227144444 29.740708277802412 20.880210610179343 60-61 20.174831728294585 28.39893825568162 30.88692823525841 20.53930178076538 62-63 20.59631958274212 28.339275461851223 30.683535115398275 20.380869840008387 64-65 20.47631756882547 28.75746358203753 30.192060810652254 20.574158038484754 66-67 20.037334697203793 29.19658772679949 30.215406500208285 20.55067107578843 68-69 20.291837162015568 28.473627249655646 30.95250558787469 20.282030000454093 70-71 20.081456689247936 28.45808262756998 30.882626307575535 20.577834375606546 72-73 20.410563680321168 28.93544968365808 29.667713923750398 20.986272712270353 74-75 19.805099485301465 29.295517545088213 30.314982217254983 20.58440075235534 76-77 20.19318447089247 28.798993147671464 30.299195607099787 20.70862677433628 78-79 19.97934225644389 28.711040659646 30.232116439333446 21.07750064457667 80-81 19.88885885243214 29.314862163951496 29.945627755688463 20.850651227927898 82-83 19.7844294890447 29.377692675921494 29.940616425284013 20.897261409749795 84-85 19.766282769689973 28.913136660440465 29.959607905712147 21.360972664157412 86-87 19.648196695973702 29.32411727694569 29.60107453911114 21.426611487969474 88-89 20.009611566920064 28.66606031195667 29.854334075163578 21.469994045959684 90-91 19.718240671877037 28.89622569781991 30.299140097764095 21.08639353253896 92-93 19.40350763019388 29.39850781479282 29.994981411825222 21.203003143188077 94-95 19.11956209199718 29.28740236858386 29.943624706545258 21.649410832873702 96-97 19.399342860744554 28.895814139594737 29.7977656763586 21.9070773233021 98-99 19.699417096094656 28.635858415808123 29.770217953740683 21.894506534356534 100-101 19.435574636810713 28.63032056779841 30.07941680697564 21.85468798841524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4074.0 1 2723.5 2 1985.5 3 2502.0 4 2067.0 5 1703.5 6 2132.5 7 3375.0 8 4530.5 9 5076.5 10 4716.0 11 3951.5 12 3654.0 13 3625.0 14 3805.5 15 4013.0 16 4206.0 17 4471.5 18 4820.5 19 5143.5 20 5640.0 21 6301.5 22 6998.0 23 8031.0 24 9242.0 25 10796.5 26 13032.0 27 15449.0 28 17930.5 29 20946.5 30 24601.5 31 28982.5 32 34643.0 33 41537.0 34 49132.0 35 56498.0 36 62995.0 37 66183.0 38 64316.5 39 60030.0 40 56363.5 41 54602.5 42 53237.5 43 51965.5 44 51601.5 45 51184.5 46 50584.5 47 51890.0 48 57115.0 49 55909.0 50 48320.0 51 43563.0 52 39798.5 53 37333.5 54 34827.5 55 30951.5 56 27103.0 57 23968.0 58 20962.5 59 17963.0 60 15226.0 61 12354.5 62 9715.5 63 7403.5 64 5376.0 65 3949.0 66 2945.5 67 2269.5 68 1613.5 69 1047.0 70 748.0 71 593.0 72 481.5 73 392.5 74 294.5 75 227.0 76 165.0 77 90.5 78 53.5 79 28.0 80 12.5 81 11.0 82 8.5 83 3.0 84 2.5 85 2.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024028759421432525 2 0.018960192980974105 3 0.0 4 0.0 5 6.25748943266472E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0673681312320683E-4 18-19 0.0013453602280229149 20-21 3.12874471633236E-5 22-23 0.0 24-25 0.0 26-27 4.3802426028653047E-4 28-29 0.017865132330257775 30-31 0.004849554310315159 32-33 0.008916922441547227 34-35 0.010387432458223435 36-37 0.09783584727971291 38-39 0.10058914263008538 40-41 0.11720277707381022 42-43 0.10011983092263553 44-45 0.09505126448217711 46-47 0.09633404981587337 48-49 0.09930635729638912 50-51 0.09761683514956963 52-53 0.07402609998842365 54-55 0.06288776879828044 56-57 0.03604313913214879 58-59 0.015737585923151772 60-61 0.00929237180750711 62-63 0.0020336840656160343 64-65 0.0047244045216618645 66-67 0.40898950931896616 68-69 0.7827493531320298 70-71 0.8640028534151812 72-73 0.2752043852485944 74-75 0.14370324482114533 76-77 0.014642525272435446 78-79 0.008572760522750668 80-81 0.0016895221468194745 82-83 0.0 84-85 0.0 86-87 2.815870244699124E-4 88-89 6.570363904297956E-4 90-91 0.0034103317408022728 92-93 0.0016269472524928273 94-95 0.21923114227340848 96-97 0.22514446978727665 98-99 0.013954201434842326 100-101 0.00772799944934093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1598085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.82469827347605 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19363792137504 53.281777782524756 2 11.76149818802357 15.24871142565326 3 2.9558361039834247 5.748335507597174 4 1.0764344669977994 2.7911815813720944 5 0.519113243407649 1.6825679686833184 6 0.29764362551400303 1.1576794930181247 7 0.20121911777212018 0.9130778017502913 8 0.1506779640893518 0.7814122846843111 9 0.10659742135616537 0.6219131108529612 >10 0.6532509975879696 7.899809321970371 >50 0.05447735582592351 2.42013478868485 >100 0.0265939634208439 3.184227738124286 >500 0.0015585190431985127 0.7047510680126564 >1k 0.0010714818421989774 1.3863914030360975 >5k 1.9481488039981408E-4 0.771178639336849 >10k+ 1.9481488039981408E-4 1.4068500846986816 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11433 0.7154187668365575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10847 0.6787498787611422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7012 0.43877515901845027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5201 0.32545202539289214 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3163 0.19792439075518511 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 0.19304354899770665 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2990 0.18709893403667516 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2720 0.17020371256848038 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2130 0.13328452491575854 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1620 0.10137132880916847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.38623414899708E-5 0.0 18-19 0.0 0.0 0.0 1.5643723581661802E-4 0.0 20-21 0.0 0.0 0.0 4.0673681312320683E-4 0.0 22-23 0.0 0.0 0.0 0.0010011983092263555 0.0 24-25 0.0 0.0 0.0 0.0015330849110028566 0.0 26-27 0.0 0.0 0.0 0.0024717083259025645 0.0 28-29 0.0 0.0 0.0 0.004317667708538657 0.0 30-31 0.0 0.0 0.0 0.008322460945444077 0.0 32-33 0.0 0.0 0.0 0.018616031062177546 0.0 34-35 0.0 0.0 0.0 0.03507322827008576 0.0 36-37 0.0 0.0 0.0 0.06226201985501397 0.0 38-39 0.0 0.0 0.0 0.09899348282475588 0.0 40-41 0.0 0.0 0.0 0.13922913987679003 0.0 42-43 0.0 0.0 0.0 0.18090401949833707 0.0 44-45 0.0 0.0 0.0 0.23252830731782104 0.0 46-47 0.0 0.0 0.0 0.2983570961494539 0.0 48-49 0.0 0.0 0.0 0.3678778037463589 0.0 50-51 0.0 0.0 0.0 0.44109043010853616 0.0 52-53 0.0 0.0 0.0 0.5174005137398825 0.0 54-55 0.0 0.0 0.0 0.6056936896347817 0.0 56-57 0.0 0.0 0.0 0.706564419289337 0.0 58-59 0.0 0.0 0.0 0.8105326062130612 3.12874471633236E-5 60-61 0.0 0.0 0.0 0.9171289386985048 6.25748943266472E-5 62-63 0.0 0.0 0.0 1.0221296113786187 6.25748943266472E-5 64-65 0.0 0.0 0.0 1.127537020871856 6.25748943266472E-5 66-67 0.0 0.0 0.0 1.2427061138800501 6.25748943266472E-5 68-69 0.0 0.0 0.0 1.3876921440348917 6.25748943266472E-5 70-71 0.0 0.0 0.0 1.5700979609970684 6.25748943266472E-5 72-73 0.0 0.0 0.0 1.766238967263944 6.25748943266472E-5 74-75 0.0 0.0 0.0 1.9808082799100175 6.25748943266472E-5 76-77 0.0 0.0 0.0 2.2199069511321365 6.25748943266472E-5 78-79 0.0 0.0 0.0 2.49877196769884 6.25748943266472E-5 80-81 0.0 0.0 0.0 2.806233710972821 6.25748943266472E-5 82-83 0.0 0.0 0.0 3.1180131219553404 6.25748943266472E-5 84-85 0.0 0.0 0.0 3.427039237587487 6.25748943266472E-5 86-87 0.0 0.0 0.0 3.7573095298435315 6.25748943266472E-5 88-89 0.0 0.0 0.0 4.113172953879174 6.25748943266472E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10520 0.0 31.846617 1 TACCTGG 1520 0.0 30.235685 2 GTACCTG 1775 0.0 27.497835 1 GTATCAA 14840 0.0 26.822855 1 GTACATG 12160 0.0 25.447155 1 TACATGG 12190 0.0 25.34169 2 ACCTGGG 1730 0.0 24.921453 3 CGTTACG 60 1.727455E-4 23.718018 34-35 ACATGGG 12205 0.0 23.679369 3 CATGGGT 1620 0.0 23.396618 4 ATAACGC 105 0.0022296275 22.561024 3 TATCAAC 18040 0.0 22.035206 2 GAGTACT 10370 0.0 21.884409 12-13 ATCAACG 18205 0.0 21.652636 3 TAGGACC 1035 0.0 21.514713 4 TCAACGC 18425 0.0 21.36838 4 CGTACAC 135 3.8632652E-4 21.056953 3 CAACGCA 18750 0.0 20.997995 5 AACGCAG 19245 0.0 20.531763 6 CATGGGG 4415 0.0 19.959991 4 >>END_MODULE