FastQCFastQC Report
Fri 27 May 2016
SRR939494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591574
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.5140822858377933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64280.4038769168131674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43260.27180640045640353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34530.21695503947664388No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.15670022254698807No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20010.12572459715979276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.11617430292276702No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16920.10630985427004964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13700.033.659382
GTACCTG16650.030.0909181
GGTATCA104000.029.1479431
GTATCAA145950.028.2597271
GAACCGT1456.46176E-726.8135346
ACCTGGG17850.025.6338463
GTACATG106200.025.4943371
TACATGG106550.024.4478072
TATCAAC171700.023.9998112
ATCAACG177750.023.1407073
TCAACGC182200.022.9560244
GAGTACT87800.022.86776412-13
ACATGGG109650.022.7974933
CAACGCA183300.022.7148025
CATGGGG37950.021.9923554
AACGCAG187150.021.8392756
GTACTTT101650.020.32749614-15
ACGCAGA198550.020.1943657
CATGGGA63900.020.1515484
AGTACTT91100.020.08386812-13
Produced by FastQC (version 0.11.5)