FastQCFastQC Report
Fri 27 May 2016
SRR939494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591574
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112190.7048996779288931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104730.6580278391077009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.4223491964558355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53180.33413463652962416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.19018908325971648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.18855548029811997No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28270.17762290663205102No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26280.16511956088752391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.12547327362723945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG14900.033.4496352
GGTATCA113350.029.6948661
ACCTGGG17700.028.1501963
GTACCTG18650.027.49171
CGGCATA350.00847570227.08884812-13
GTATCAA155850.026.1968061
ATAACGC1301.0101188E-525.5517183
GTACATG121250.024.0404871
TACATGG121400.023.4987142
CATTCCG1452.3672726E-522.890449
ACATGGG123150.022.3105133
GAGTACT100550.022.08798412-13
TATCAAC186600.021.6728652
ATCAACG187650.021.5454923
TCAACGC189200.021.3736884
GTAGGAC25500.021.2143693
CAACGCA191200.021.1536465
TAGGACC9600.020.7653434
AACGCAG195300.020.7581186
CATGGGG44850.020.6363644
Produced by FastQC (version 0.11.5)