##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591574 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.933813319393256 31.0 31.0 31.0 30.0 34.0 2 31.1655455542752 31.0 31.0 34.0 30.0 34.0 3 31.162435425559856 31.0 31.0 34.0 28.0 34.0 4 35.06722401848736 35.0 35.0 37.0 32.0 37.0 5 34.660877219658026 35.0 35.0 37.0 32.0 37.0 6 34.73631072133624 35.0 35.0 37.0 32.0 37.0 7 34.58854253713619 35.0 35.0 37.0 30.0 37.0 8 34.49462795949167 35.0 35.0 37.0 30.0 37.0 9 35.985652567835366 37.0 35.0 39.0 30.0 39.0 10-11 35.77890283455246 37.0 35.0 39.0 30.0 39.0 12-13 35.77311014128152 37.0 35.0 39.0 30.0 39.0 14-15 36.54467212960252 38.0 35.0 40.0 30.5 41.0 16-17 36.493299714622125 38.0 35.0 40.0 30.0 41.0 18-19 36.56014769027391 38.0 35.0 40.0 30.0 41.0 20-21 36.48136216098026 38.0 35.0 40.0 30.0 41.0 22-23 36.3069263508954 38.0 34.0 40.0 30.0 41.0 24-25 36.15495289568691 38.0 34.0 40.0 29.0 41.0 26-27 35.68784674793632 38.0 34.0 40.0 27.5 41.0 28-29 35.47059451838243 38.0 34.0 40.0 27.0 41.0 30-31 35.153979645307096 37.5 34.0 40.0 27.0 41.0 32-33 34.850967658431216 37.0 33.0 40.0 26.0 41.0 34-35 35.03079969891441 37.5 33.0 40.0 27.0 41.0 36-37 34.81875395049178 38.0 33.0 40.0 25.0 41.0 38-39 34.6092098136813 37.0 33.0 40.0 25.0 41.0 40-41 34.404247619023685 37.0 33.0 40.0 24.5 41.0 42-43 34.24477749699354 37.0 33.0 40.0 24.0 41.0 44-45 33.80845659705423 37.0 33.0 40.0 23.5 41.0 46-47 33.31939671042628 37.0 32.0 40.0 21.0 41.0 48-49 32.86434718084111 37.0 31.0 40.0 18.0 41.0 50-51 32.63125057333181 37.0 31.0 40.0 15.5 41.0 52-53 32.53004384339025 37.0 31.0 39.0 15.0 41.0 54-55 32.22516577928516 36.0 31.0 39.0 14.0 40.0 56-57 31.759270068498232 36.0 30.0 39.0 10.5 40.0 58-59 31.336600748692803 35.0 29.0 39.0 8.0 40.0 60-61 31.011729897573094 35.0 29.0 39.0 8.0 40.0 62-63 30.877578108212376 35.0 29.0 38.0 7.5 40.0 64-65 30.44502706126137 34.5 28.0 38.0 6.0 40.0 66-67 29.643823347202204 34.0 27.0 38.0 2.0 40.0 68-69 26.36176828724269 31.0 18.0 36.0 2.0 39.0 70-71 25.12797802678355 30.5 13.0 35.0 2.0 39.0 72-73 26.099759420548466 31.0 19.5 35.0 2.0 38.0 74-75 26.810296285312525 31.0 22.5 35.0 2.0 38.0 76-77 26.421419299385388 30.5 23.0 34.5 2.0 37.0 78-79 27.48351726027191 32.0 26.0 35.0 2.0 37.0 80-81 27.491422956142785 33.0 26.0 35.0 2.0 37.0 82-83 27.181800217897504 32.5 25.5 35.0 2.0 36.5 84-85 26.81218246842434 32.0 25.0 35.0 2.0 36.0 86-87 26.346967216101795 32.0 24.5 34.5 2.0 36.0 88-89 25.847910307657703 32.0 23.0 34.0 2.0 35.0 90-91 25.259984141485095 31.0 20.0 34.0 2.0 35.0 92-93 24.72495718075314 31.0 19.0 34.0 2.0 35.0 94-95 23.92454890567451 31.0 10.0 34.0 2.0 35.0 96-97 23.235188561763387 30.5 2.0 34.0 2.0 35.0 98-99 22.405768440550048 30.0 2.0 34.0 2.0 35.0 100-101 21.033367282953854 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2669944800971855 1101 2 0.25741904976158736 1101 3 0.31591244848671707 1101 4 0.3194595380620413 1101 5 0.48008197384234563 1101 6 0.42245622486063894 1101 7 0.5308963135475295 1101 8 0.712663044917484 1101 9 0.6723957251975179 1101 10-11 0.7285693199235652 1101 12-13 0.6573144828146056 1101 14-15 0.5578506716302982 1101 16-17 0.6640620330947229 1101 18-19 0.5002141772868569 1101 20-21 0.6751413374557274 1101 22-23 0.6501882865227557 1101 24-25 0.7962342144307613 1101 26-27 0.6705272226086763 1101 28-29 0.8283475465830605 1101 30-31 0.5016452096329687 1101 32-33 0.7759873787323954 1101 34-35 0.7926319631330898 1101 36-37 0.9242385787454666 1101 38-39 0.8229760480255024 1101 40-41 0.7048582235906835 1101 42-43 0.39373417959841106 1101 44-45 0.6402112593086073 1101 46-47 0.49993532787527073 1101 48-49 0.5445231211154962 1101 50-51 0.3921453767697187 1101 52-53 0.019361111773321227 1101 54-55 0.01735563287608244 1101 56-57 0.3388880111903845 1101 58-59 0.39681060382210376 1101 60-61 0.3513488691471167 1101 62-63 0.016223592218089777 1101 64-65 -0.02488594691461188 1101 66-67 -0.07768845990365492 1101 68-69 -7.119020670988913 1101 70-71 -8.052878132757048 1101 72-73 -4.764121365548128 1101 74-75 -2.773234963401354 1101 76-77 -0.2129393181211583 1101 78-79 0.32776925014798053 1101 80-81 0.11991707575883837 1101 82-83 -0.17235885123098882 1101 84-85 -0.2910148150491203 1101 86-87 -0.17867894274880314 1101 88-89 -0.2247410772120162 1101 90-91 -0.3019949725949296 1101 92-93 -0.43011421748804324 1101 94-95 -0.38065197899150505 1101 96-97 -0.764367980094665 1101 98-99 -0.5759141154917486 1101 100-101 -0.3921338252331452 1102 1 0.3243047111202877 1102 2 0.2975008079374071 1102 3 0.376677524994367 1102 4 0.20701129323756362 1102 5 0.5290213677817377 1102 6 0.536001829421096 1102 7 0.3968114050566811 1102 8 0.5569714757605695 1102 9 0.6249969853235342 1102 10-11 0.6129422572172913 1102 12-13 0.54720159652598 1102 14-15 0.694011037666904 1102 16-17 0.6080381807094852 1102 18-19 0.48416669753888897 1102 20-21 0.5855321265346305 1102 22-23 0.717440761770284 1102 24-25 0.4662522162309344 1102 26-27 0.5440687017565935 1102 28-29 0.7133107929076914 1102 30-31 0.5010819033023353 1102 32-33 0.7474845284473659 1102 34-35 0.4735350534421201 1102 36-37 0.6545952394115417 1102 38-39 0.6762122466453633 1102 40-41 0.5008783256008869 1102 42-43 0.5611325444348978 1102 44-45 0.6062069838810658 1102 46-47 0.7705642657690603 1102 48-49 0.6035007688802665 1102 50-51 0.6026588031123552 1102 52-53 0.4885862842905695 1102 54-55 0.27593574088687234 1102 56-57 0.4809840957988456 1102 58-59 0.2744527430360222 1102 60-61 0.5540420134615509 1102 62-63 0.6650065955184203 1102 64-65 0.17076474315439683 1102 66-67 0.0704971086531998 1102 68-69 -7.225077526674482 1102 70-71 -7.8679415140951825 1102 72-73 -1.453082886700244 1102 74-75 0.2817800380987947 1102 76-77 0.7193536611767719 1102 78-79 0.19207280550351413 1102 80-81 0.0013283418854470597 1102 82-83 0.12550756041017408 1102 84-85 -0.026835772144831793 1102 86-87 -0.0272924290974359 1102 88-89 -0.014331161220418664 1102 90-91 -0.20333198129580055 1102 92-93 -0.11592242330862845 1102 94-95 0.09746983318971303 1102 96-97 -0.03522944124078009 1102 98-99 -0.21996077015721838 1102 100-101 -0.5202818900396267 1103 1 0.3199895741476837 1103 2 0.18135567332796398 1103 3 0.2411635368828975 1103 4 0.2979781907671466 1103 5 0.3664796942982562 1103 6 0.3822794829812963 1103 7 0.3036256785656164 1103 8 0.26105138705104025 1103 9 0.4685493657565374 1103 10-11 0.5501488159055512 1103 12-13 0.46203590239207415 1103 14-15 0.5716460561177641 1103 16-17 0.6459702306631243 1103 18-19 0.6451046917582275 1103 20-21 0.5914383998254351 1103 22-23 0.48159193153632174 1103 24-25 0.47202578878914636 1103 26-27 0.7362231799518639 1103 28-29 0.4954345499744619 1103 30-31 0.4685653157107552 1103 32-33 0.7561646106127498 1103 34-35 0.8416632459854299 1103 36-37 0.8568317012100906 1103 38-39 1.1074749032049596 1103 40-41 0.9841922082788912 1103 42-43 0.8373820218121466 1103 44-45 1.160210910368498 1103 46-47 0.9921708535424116 1103 48-49 0.9737190888526399 1103 50-51 1.0232315976443545 1103 52-53 0.6782287194399004 1103 54-55 0.6424220799827012 1103 56-57 0.7464691806000658 1103 58-59 0.8363212784198595 1103 60-61 0.7070270528173133 1103 62-63 0.48517256232506156 1103 64-65 0.5416920122460311 1103 66-67 0.24670223126504354 1103 68-69 -6.711396626490881 1103 70-71 -7.849237982074317 1103 72-73 -1.0412238039713344 1103 74-75 0.5590458576621558 1103 76-77 0.7409749732779858 1103 78-79 0.5969062024604881 1103 80-81 0.32387201496901596 1103 82-83 0.10776674493690663 1103 84-85 0.0631202366534076 1103 86-87 0.18780673288834748 1103 88-89 0.18888492283549851 1103 90-91 0.04619993050365778 1103 92-93 -0.006524121150874862 1103 94-95 0.1218084200178069 1103 96-97 0.20555876475438595 1103 98-99 0.03655410868519482 1103 100-101 0.2941790359569936 1104 1 0.18448574221040914 1104 2 0.16190065051906544 1104 3 0.27984163361302095 1104 4 0.1312852011478327 1104 5 0.38636886876560084 1104 6 0.38723362792562455 1104 7 0.43677992138883326 1104 8 0.4821347972875287 1104 9 0.5315141045679255 1104 10-11 0.4895735047576366 1104 12-13 0.3533605890486058 1104 14-15 0.5664943119683628 1104 16-17 0.5458894203788986 1104 18-19 0.5242729549178691 1104 20-21 0.5154652237404633 1104 22-23 0.4402152599993272 1104 24-25 0.6878814960420314 1104 26-27 0.7083386742083846 1104 28-29 0.6284859208100926 1104 30-31 0.430885130378293 1104 32-33 0.6924864961766062 1104 34-35 0.31281092908162833 1104 36-37 0.7283378604268833 1104 38-39 0.674398000285656 1104 40-41 0.5136606947468891 1104 42-43 0.3464768970519714 1104 44-45 0.6751207973403339 1104 46-47 0.6540943720264423 1104 48-49 0.540623948730719 1104 50-51 0.4758504599403679 1104 52-53 0.3819432303747874 1104 54-55 0.44985545753386447 1104 56-57 0.6264386412533867 1104 58-59 0.6634531365818717 1104 60-61 0.7478279245201875 1104 62-63 0.5347035652153913 1104 64-65 0.48657741533070364 1104 66-67 0.3013392967681163 1104 68-69 -6.450146397197898 1104 70-71 -4.0403539501439845 1104 72-73 0.2636349409266714 1104 74-75 0.9531771258595185 1104 76-77 1.0724469315427712 1104 78-79 0.594174340332323 1104 80-81 0.32598398926836936 1104 82-83 0.07419311727754874 1104 84-85 0.35152707712710907 1104 86-87 0.1412120966798156 1104 88-89 0.21716037754384843 1104 90-91 0.2717762438124218 1104 92-93 0.2998233460735058 1104 94-95 0.36600190717633296 1104 96-97 0.34975678465453797 1104 98-99 0.27397468832369753 1104 100-101 0.5350545916604901 1105 1 0.0811223650111117 1105 2 0.15217718846744432 1105 3 0.15908065032432006 1105 4 0.18816217487624698 1105 5 0.29062204352875654 1105 6 0.2543399141006475 1105 7 0.12897907478532744 1105 8 0.4093144173718031 1105 9 0.5811536741631471 1105 10-11 0.34310485884331143 1105 12-13 0.37818037384622016 1105 14-15 0.2925888013666196 1105 16-17 0.38511874242419 1105 18-19 0.47666590564784883 1105 20-21 0.5537255591183126 1105 22-23 0.5507392518980367 1105 24-25 0.40653940871325034 1105 26-27 0.5214427910819026 1105 28-29 0.35133845630121385 1105 30-31 0.5279565733477369 1105 32-33 0.46330123206895024 1105 34-35 0.5326734759951748 1105 36-37 0.5182875633477622 1105 38-39 0.40106601076741555 1105 40-41 0.4757627095096595 1105 42-43 0.29834192042476104 1105 44-45 0.44684260841912504 1105 46-47 0.614303047312724 1105 48-49 0.6173725881179379 1105 50-51 0.34805844412174736 1105 52-53 0.2511108329126728 1105 54-55 0.3911115406968193 1105 56-57 0.44995252574288713 1105 58-59 0.6221404212115083 1105 60-61 0.7555476622842683 1105 62-63 0.4834154224638354 1105 64-65 0.5145911606393518 1105 66-67 0.4515466282393028 1105 68-69 -3.17398999631866 1105 70-71 -3.059668892407931 1105 72-73 0.7347621138276814 1105 74-75 1.1536647387639825 1105 76-77 1.1395584969913628 1105 78-79 0.5360375399560553 1105 80-81 0.41727702734633 1105 82-83 0.4532617574326636 1105 84-85 0.3062885019550521 1105 86-87 0.32317425868632554 1105 88-89 0.5929246723768813 1105 90-91 0.28803640641404726 1105 92-93 0.24910872440544907 1105 94-95 0.3397399208256573 1105 96-97 0.12372948601083067 1105 98-99 0.1784966261050549 1105 100-101 0.3262665208990292 1106 1 0.15308784131955377 1106 2 0.18102074321590322 1106 3 0.1240800470225416 1106 4 0.16248747115135131 1106 5 0.3794580620605714 1106 6 0.1667202732910198 1106 7 0.11290630647950195 1106 8 0.2138008255556798 1106 9 0.16645110868131496 1106 10-11 0.4402647567336473 1106 12-13 0.03249064487364706 1106 14-15 0.32462395734540905 1106 16-17 0.4267022444460906 1106 18-19 0.3335984497680826 1106 20-21 0.43242162511884885 1106 22-23 0.2600757008300647 1106 24-25 0.33426941667413956 1106 26-27 0.4868146170910208 1106 28-29 0.49743977864434186 1106 30-31 0.5444236785709791 1106 32-33 0.6822354034187796 1106 34-35 0.5280189522092442 1106 36-37 0.5880511566805708 1106 38-39 0.7594727682866136 1106 40-41 0.7114873760590328 1106 42-43 0.7397223894902112 1106 44-45 0.8344099069011648 1106 46-47 0.8003695736358907 1106 48-49 0.8005147860103605 1106 50-51 0.8231334861000974 1106 52-53 0.6479450489540355 1106 54-55 0.571558597426737 1106 56-57 0.6823137006107487 1106 58-59 0.7336157603243976 1106 60-61 0.832025094416192 1106 62-63 0.5070166554913342 1106 64-65 0.49258749768753773 1106 66-67 0.8156945268279863 1106 68-69 -3.1776109866368465 1106 70-71 -3.1865700513540176 1106 72-73 1.1071418798274806 1106 74-75 1.5033585041287978 1106 76-77 1.4552492977514575 1106 78-79 0.8941342205829201 1106 80-81 0.7937103746331218 1106 82-83 0.7352375943537872 1106 84-85 0.7322143342587282 1106 86-87 0.7513983674343336 1106 88-89 0.6533165286791593 1106 90-91 0.7392540648886161 1106 92-93 0.6080982242581605 1106 94-95 0.774872003470584 1106 96-97 0.9612970541249517 1106 98-99 0.8824444779125713 1106 100-101 0.8777094569766781 1107 1 0.10561857973342725 1107 2 0.21641034122650282 1107 3 0.22469566257454687 1107 4 0.0011342748787939172 1107 5 0.46761238746764633 1107 6 0.3866720351277948 1107 7 0.12902994077886376 1107 8 0.16044194689527558 1107 9 0.25301291100369383 1107 10-11 0.1750955418888296 1107 12-13 0.16901093074407214 1107 14-15 0.10443019272109666 1107 16-17 0.11925914952168881 1107 18-19 0.24364137128363694 1107 20-21 0.3323270834025891 1107 22-23 0.342367769069547 1107 24-25 0.23449154381332704 1107 26-27 0.45077297072363365 1107 28-29 0.25362488271083805 1107 30-31 0.2797326442888419 1107 32-33 0.2699076649215968 1107 34-35 0.4688292361450621 1107 36-37 0.4952897760184882 1107 38-39 0.6305564182888048 1107 40-41 0.49402254148233027 1107 42-43 0.2926322126498917 1107 44-45 0.5975606666945978 1107 46-47 0.6272442038358648 1107 48-49 0.7148710409439687 1107 50-51 0.7043630720793956 1107 52-53 0.3839346585440495 1107 54-55 0.39506425227816777 1107 56-57 0.7516874800058844 1107 58-59 0.9100864523923633 1107 60-61 0.9230610358017373 1107 62-63 0.5884250870026051 1107 64-65 0.42466432289339195 1107 66-67 0.4355535121037626 1107 68-69 -2.6185524216092304 1107 70-71 -2.6367647832834145 1107 72-73 1.3241135300217195 1107 74-75 1.2657426569884187 1107 76-77 0.7554651409378863 1107 78-79 0.716362785596413 1107 80-81 0.6205110448272748 1107 82-83 0.6884438576895384 1107 84-85 0.4924951280984793 1107 86-87 0.4297734855232207 1107 88-89 0.4835553683084477 1107 90-91 0.5134342208718614 1107 92-93 0.4121045618716437 1107 94-95 0.596649218281506 1107 96-97 0.45064204736793556 1107 98-99 0.5906817867992089 1107 100-101 0.6339782294029774 1108 1 -0.029250316591507897 1108 2 -0.10524733861121405 1108 3 9.033941371008325E-4 1108 4 0.015374497729034431 1108 5 -0.2286472423096768 1108 6 -0.19292352867290674 1108 7 0.041299996433636466 1108 8 0.17894441177151066 1108 9 0.2380632777009879 1108 10-11 0.19438327067731365 1108 12-13 0.02348281728463064 1108 14-15 0.18482763595381613 1108 16-17 0.0554642226010813 1108 18-19 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100-101 0.763789669468828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 24.0 4 50.0 5 146.0 6 408.0 7 920.0 8 1858.0 9 3193.0 10 5086.0 11 7652.0 12 10117.0 13 12355.0 14 14850.0 15 18890.0 16 22688.0 17 25179.0 18 21621.0 19 17085.0 20 16680.0 21 16775.0 22 17475.0 23 19912.0 24 23801.0 25 29228.0 26 36890.0 27 46110.0 28 56124.0 29 67034.0 30 78588.0 31 91796.0 32 106246.0 33 123954.0 34 143728.0 35 163649.0 36 169735.0 37 147993.0 38 69153.0 39 4575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20080629196819 21.154009307236205 15.667516111699403 20.977668289096204 2 15.168924502583781 22.550561720081973 40.96123041112367 21.319283366210577 3 18.94214614322282 28.25896823372259 31.117920633943257 21.680964989111327 4 12.368877682901443 18.964568437060343 37.86945220289266 30.797101677145548 5 11.774381838356243 39.660549870756874 34.20224255988097 14.362825731005909 6 28.005232555947764 37.845805473072566 19.10831667267749 15.040645298302183 7 25.92483918435461 35.05184176167743 21.93690020068184 17.086418853286116 8 25.285911933720957 36.027102729750546 20.4509498144604 18.236035522068093 9 26.45475485274326 17.40226970282249 21.801562478401884 34.34141296603237 10-11 23.719892081052947 27.986976079558644 28.170307585337124 20.12282425405128 12-13 26.027127862103804 25.987198835869396 28.585758500704333 19.399914801322467 14-15 22.115873971301365 27.955250588411218 27.839390439904143 22.08948500038327 16-17 19.604627652188448 30.43539589698931 29.555851700498188 20.40412475032405 18-19 19.643478159793638 27.90748012263351 33.187481680841856 19.261560036730994 20-21 21.22361259985398 27.115201680851786 32.4042740079946 19.256911711299633 22-23 20.365858753979165 27.992288120444552 32.26508030904066 19.376772816535617 24-25 20.162770715094698 27.674446003069423 33.07088741613664 19.091895865699236 26-27 19.731381818147543 27.952759492446038 32.933955088718896 19.38190360068752 28-29 19.433811053162835 27.947222634991537 32.73964714349847 19.879319168347152 30-31 19.70549852944041 27.78886395894371 32.917695786113214 19.587941725502663 32-33 19.47373083800694 28.197958986203492 32.80170675097797 19.5266034248116 34-35 19.862320384085415 28.180401012616237 32.618765319464835 19.338513283833514 36-37 19.876637654474123 27.792340994979543 32.635273631793865 19.695747718752465 38-39 20.15330357409594 28.043044498917443 32.432180857336476 19.371471069650138 40-41 20.47376688184376 27.55826193251916 32.75954871918698 19.208422466450106 42-43 19.635064128201066 28.45071095650249 32.32289599384249 19.591328921453957 44-45 19.6688736308134 28.78852571483189 31.558403012320557 19.984197642034147 46-47 20.389625456636544 28.19644009780566 30.959132463663224 20.454801981894573 48-49 20.3075798046258 28.532102938661353 30.73525010311975 20.425067153593094 50-51 20.18491743039659 29.182515811418046 30.54583307003239 20.086733688152965 52-53 20.133265562658483 29.00072517136771 30.646835885892543 20.21917338008127 54-55 19.93894291983343 28.712322963226516 30.78237359477724 20.566360522162817 56-57 20.410610485669736 29.17028827704361 29.670651852159107 20.748449385127547 58-59 20.40635356763651 28.944372503653685 29.742386330321036 20.906887598388764 60-61 20.1764545086075 28.427053844389494 30.907974072247065 20.488517574755946 62-63 20.579993163836697 28.37042328919987 30.64351042012245 20.40607312684099 64-65 20.487803192222998 28.72658245267008 30.195955101647133 20.589659253459793 66-67 20.17455128037728 29.242169057184597 29.98765700506651 20.595622657371614 68-69 20.572312818697263 28.4768117633994 30.664225449968818 20.286649967934512 70-71 20.326054958715527 28.43560100855983 30.618226135033826 20.620117897690815 72-73 20.390683668045618 28.952943557616102 29.695445554792627 20.96092721954566 74-75 20.02256213860659 29.267318280172855 30.063499627212366 20.64661995400819 76-77 19.689624565644355 28.83246462204764 30.655530795185577 20.822380017122423 78-79 19.952957603715884 28.706104734371646 30.167774701735432 21.173162960177034 80-81 19.90446865401272 29.27516753643295 29.95637188234263 20.8639919272117 82-83 19.74944566652278 29.347692910877505 29.926719282008555 20.976142140591158 84-85 19.74583603244844 28.904239898816954 29.931338281484145 21.418585787250457 86-87 19.62335957304367 29.31023920474734 29.540612907776065 21.52578831443293 88-89 19.9877165121806 28.631584899792184 29.885411708897045 21.495286879130166 90-91 19.76643359455506 28.87123184726823 30.259359747055605 21.102974811121108 92-93 19.441769633366395 29.38544832259761 29.922648128943035 21.25013391509296 94-95 19.275245584879656 29.23543137613675 29.76684291307713 21.72248012590646 96-97 19.64915434436979 28.81662266351432 29.505190025421367 22.029032966694523 98-99 19.714913844811004 28.597188476278063 29.671155221986957 22.01674245692398 100-101 19.407072450070945 28.57889299579353 30.063191312074338 21.950843242061183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4134.0 1 2786.0 2 2022.0 3 2502.0 4 2009.0 5 1667.0 6 2121.0 7 3338.0 8 4521.0 9 5040.0 10 4616.0 11 3802.5 12 3573.0 13 3580.0 14 3687.0 15 3889.5 16 4107.5 17 4399.5 18 4785.5 19 5238.5 20 5724.5 21 6337.0 22 7070.0 23 8040.5 24 9143.5 25 10660.5 26 12944.5 27 15425.0 28 17891.5 29 20690.0 30 24239.5 31 28696.5 32 34312.0 33 41032.5 34 48610.0 35 56319.0 36 62830.5 37 65837.0 38 64293.5 39 60021.0 40 56161.5 41 54463.5 42 52949.5 43 51736.0 44 51475.0 45 51052.0 46 50381.0 47 51541.5 48 56755.5 49 55597.5 50 48471.0 51 43797.5 52 39762.5 53 36826.5 54 34235.0 55 31011.5 56 27305.5 57 23872.0 58 21033.0 59 18274.0 60 15336.0 61 12361.5 62 9630.0 63 7349.5 64 5393.0 65 3975.5 66 2971.0 67 2202.5 68 1539.0 69 1047.0 70 775.0 71 588.5 72 492.0 73 415.5 74 312.0 75 238.5 76 161.0 77 87.5 78 46.5 79 27.5 80 22.0 81 12.0 82 7.5 83 5.0 84 2.5 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021299669383892925 2 0.01891209582463649 3 7.539705976599266E-4 4 0.09745069974754551 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012220606770404645 12-13 0.0 14-15 0.0 16-17 6.911397145215993E-4 18-19 1.5707720784581804E-4 20-21 0.0 22-23 2.1990809098414526E-4 24-25 0.004492408144390396 26-27 0.0036756066635921423 28-29 0.0012880331043357079 30-31 1.8849264941498165E-4 32-33 7.539705976599266E-4 34-35 0.0033300368063313426 36-37 0.08673803417246072 38-39 0.09999535051464777 40-41 0.07891558922173898 42-43 0.08306242750886858 44-45 0.08199430249551702 46-47 0.08026645320921301 48-49 0.07476875093460938 50-51 0.0746745046099019 52-53 0.05752167351313857 54-55 0.04822270280866613 56-57 0.02708011063261903 58-59 0.023467334852165216 60-61 0.014388272238676931 62-63 0.002387573559256434 64-65 0.0070684743530618115 66-67 0.15164233645435274 68-69 0.7046169389547705 70-71 0.8595578967738854 72-73 0.3509104823275575 74-75 0.0537832359664081 76-77 0.18937228177891824 78-79 0.015299320044182677 80-81 0.003424283131038833 82-83 0.001445110312181526 84-85 1.8849264941498165E-4 86-87 0.001853511052580653 88-89 4.0840074039912694E-4 90-91 0.004900808884789523 92-93 0.005057886092635341 94-95 0.0745802582851944 96-97 0.03703880561004389 98-99 0.014482518563384424 100-101 0.013571470757878679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1591574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.60727177019525 #Duplication Level Percentage of deduplicated Percentage of total 1 82.88752327790418 54.38024266051844 2 11.28429864435149 14.806640957920278 3 2.8153091294260477 5.541142535140995 4 1.0404410047008155 2.7304198302504563 5 0.501790229843834 1.6460543990496581 6 0.3025846821073123 1.1911053283507544 7 0.1968553934956757 0.9040601710349665 8 0.1486443000271278 0.7801717591176173 9 0.09939144741568909 0.5868721532010767 >10 0.642225761481111 7.883537273412074 >50 0.052255046690286445 2.3498123035565923 >100 0.02568762927568978 3.032622665011792 >500 0.0018346971721393214 0.8862361050546684 >1k 7.725040724797142E-4 1.144080193093306 >5k 1.9312601811992855E-4 0.7627623636327263 >10k+ 1.9312601811992855E-4 1.3742393016545764 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11219 0.7048996779288931 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10473 0.6580278391077009 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6722 0.4223491964558355 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5318 0.33413463652962416 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3027 0.19018908325971648 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3001 0.18855548029811997 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2827 0.17762290663205102 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2628 0.16511956088752391 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.12547327362723945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.141544156916361E-5 0.0 12-13 0.0 0.0 0.0 6.283088313832722E-5 0.0 14-15 0.0 0.0 0.0 9.424632470749083E-5 0.0 16-17 0.0 0.0 0.0 2.1990809098414526E-4 0.0 18-19 0.0 0.0 0.0 3.141544156916361E-4 0.0 20-21 0.0 0.0 0.0 4.398161819682905E-4 0.0 22-23 0.0 0.0 0.0 7.853860392290901E-4 0.0 24-25 0.0 0.0 0.0 0.0012566176627665441 0.0 26-27 0.0 0.0 0.0 0.0019791728188573074 0.0 28-29 0.0 0.0 0.0 0.0034871140141771607 0.0 30-31 0.0 0.0 0.0 0.00835650745739752 0.0 32-33 0.0 0.0 0.0 0.018817849499929002 0.0 34-35 0.0 0.0 0.0 0.03509104823275575 0.0 36-37 0.0 0.0 0.0 0.06298796034617303 0.0 38-39 0.0 0.0 0.0 0.10301123290528746 0.0 40-41 0.0 0.0 0.0 0.14413404591932263 0.0 42-43 0.0 0.0 0.0 0.18425156480314456 0.0 44-45 0.0 0.0 0.0 0.23888301769192008 0.0 46-47 0.0 0.0 0.0 0.30149399273926314 0.0 48-49 0.0 0.0 0.0 0.369822578152194 0.0 50-51 0.0 0.0 0.0 0.43906221137063056 0.0 52-53 0.0 0.0 0.0 0.5144592711366233 0.0 54-55 0.0 0.0 0.0 0.6018884450236055 0.0 56-57 0.0 0.0 0.0 0.7042085382143715 0.0 58-59 0.0 0.0 0.0 0.8116179329393418 0.0 60-61 0.0 0.0 0.0 0.9208180078337544 0.0 62-63 0.0 0.0 0.0 1.0339764283658819 0.0 64-65 0.0 0.0 0.0 1.1475432496384084 0.0 66-67 0.0 0.0 0.0 1.2769120380202241 0.0 68-69 0.0 0.0 0.0 1.4271406796039643 0.0 70-71 0.0 0.0 0.0 1.6073396524446868 0.0 72-73 0.0 0.0 0.0 1.8083042321626266 0.0 74-75 0.0 0.0 0.0 2.0226517899890295 0.0 76-77 0.0 0.0 0.0 2.2651475834614034 0.0 78-79 0.0 0.0 0.0 2.542451686192411 0.0 80-81 0.0 0.0 0.0 2.845359373802286 0.0 82-83 0.0 0.0 0.0 3.157157631376235 0.0 84-85 0.0 0.0 0.0 3.482181789850802 0.0 86-87 0.0 0.0 0.0 3.8106930623395456 0.0 88-89 0.0 0.0 0.0 4.169143250643702 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1490 0.0 33.449635 2 GGTATCA 11335 0.0 29.694866 1 ACCTGGG 1770 0.0 28.150196 3 GTACCTG 1865 0.0 27.4917 1 CGGCATA 35 0.008475702 27.088848 12-13 GTATCAA 15585 0.0 26.196806 1 ATAACGC 130 1.0101188E-5 25.551718 3 GTACATG 12125 0.0 24.040487 1 TACATGG 12140 0.0 23.498714 2 CATTCCG 145 2.3672726E-5 22.89044 9 ACATGGG 12315 0.0 22.310513 3 GAGTACT 10055 0.0 22.087984 12-13 TATCAAC 18660 0.0 21.672865 2 ATCAACG 18765 0.0 21.545492 3 TCAACGC 18920 0.0 21.373688 4 GTAGGAC 2550 0.0 21.214369 3 CAACGCA 19120 0.0 21.153646 5 TAGGACC 960 0.0 20.765343 4 AACGCAG 19530 0.0 20.758118 6 CATGGGG 4485 0.0 20.636364 4 >>END_MODULE