FastQCFastQC Report
Fri 27 May 2016
SRR939493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908295
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153600.8049069981318402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.6372704429870644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.43855902782326633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81760.4284452875472608No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56900.2981719283444122No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA45220.23696545869480345No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.1618198444160887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.15521709169703846No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27820.14578458781268094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.14164476666343517No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.11114633743734591No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19150.10035136076969231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG134650.026.518711
CATTCCG4350.025.9648679
TACATGG136950.025.504382
AGGCTTA9500.024.5354586
ACATGGG138550.024.2774433
GAGTACT120350.023.62972312-13
CATGGGA100750.023.1917764
GTATAGG10050.022.9546411
GGACAAT8850.022.4966136
GTACTTT131900.022.06906114-15
ATGGGAT43000.021.8032955
AGTACTT129200.021.7329712-13
CATGGGG29350.021.2077474
GACAATG10450.020.9216617
GTATCAA282650.020.9191271
ACTTTTT153800.020.75541116-17
CTCCCGC4800.020.2436477
TAGGACA13950.019.2208734
TACCTGG9450.019.1267282
GATATAG12900.018.66081
Produced by FastQC (version 0.11.5)