FastQCFastQC Report
Fri 27 May 2016
SRR939493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908295
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204711.0727377056482357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189160.9912513526472584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133390.6990009406302485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125030.6551922003673436No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA75910.3977896499230989No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA64070.33574473548376954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47520.24901810254703807No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.24859888015217774No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT46430.24330619741706602No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46140.24178651623569733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39760.20835353024558573No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30530.15998574643857474No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28270.14814271378377034No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27990.14667543540175915No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27320.14316444784480387No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25740.13488480554631227No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.12728639963946875No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.11575778378080959No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.11072711504248557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG4700.036.285649
TACGCGG452.1742613E-532.1557264-65
CTAACGC1057.0353184E-527.0702383
TACCTGG9300.027.003842
TAGGCAT7500.025.2655585
GTACATG149950.024.2751081
TACATGG152300.023.240922
TTAGGCA8200.023.1087424
ACATGGG150500.022.4431173
CATGGGG30100.022.19134
GAGTACT139000.022.18746812-13
GTATAGG10300.022.0876961
GTATCGA1104.8961738E-821.88436162-63
CCTTTAG7950.021.4625741
GGACAAT8950.021.1722556
GTACTTT148050.020.8631914-15
AGTACTT145500.020.8218412-13
CATGGGA115400.020.5665824
CTTTAGG9250.020.490422
CTTACTA12050.020.049959
Produced by FastQC (version 0.11.5)