##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1908295 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.980766600551803 31.0 31.0 31.0 30.0 34.0 2 31.22148619579258 31.0 31.0 34.0 30.0 34.0 3 31.183409797751395 31.0 31.0 34.0 28.0 34.0 4 35.12405000275115 35.0 35.0 37.0 32.0 37.0 5 34.772178305765095 35.0 35.0 37.0 32.0 37.0 6 34.75042171152783 35.0 35.0 37.0 32.0 37.0 7 34.637137339876695 35.0 35.0 37.0 30.0 37.0 8 34.668880335587524 35.0 35.0 37.0 31.0 37.0 9 35.96352765164715 37.0 35.0 39.0 30.0 39.0 10-11 35.83667540920036 37.0 35.0 39.0 30.0 39.0 12-13 35.81338446099791 37.0 35.0 39.0 30.0 39.0 14-15 36.56620517268033 38.0 35.0 40.0 30.0 41.0 16-17 36.59584367196895 38.0 35.5 40.0 30.5 41.0 18-19 36.66100943512402 38.0 35.0 40.0 30.5 41.0 20-21 36.61162084478553 38.0 35.0 40.0 30.0 41.0 22-23 36.463004409695564 38.0 35.0 40.0 30.0 41.0 24-25 36.239756169774594 38.0 34.5 40.0 29.5 41.0 26-27 35.83098787137209 38.0 34.0 40.0 28.0 41.0 28-29 35.55489062225704 38.0 34.0 40.0 27.0 41.0 30-31 35.3019740134518 37.5 34.0 40.0 27.0 41.0 32-33 35.03100490228188 37.0 33.0 40.0 27.0 41.0 34-35 34.91380787561671 37.0 33.0 40.0 26.5 41.0 36-37 34.818716445832536 37.5 33.0 40.0 25.0 41.0 38-39 34.757289884425624 37.0 33.0 40.0 25.0 41.0 40-41 34.541354193141 37.0 33.0 40.0 24.5 41.0 42-43 34.23041956301306 37.0 33.0 40.0 24.0 41.0 44-45 34.01431251457453 37.0 33.0 40.0 24.0 41.0 46-47 33.613115372623206 37.0 32.5 40.0 22.5 41.0 48-49 33.26428670619585 37.0 32.0 40.0 20.5 41.0 50-51 33.029721033697626 37.0 31.5 40.0 19.5 41.0 52-53 32.665627693831404 36.5 31.0 40.0 18.0 41.0 54-55 32.48629090366008 36.0 31.0 39.0 17.0 41.0 56-57 32.15052913726652 36.0 31.0 39.0 14.5 40.0 58-59 31.780549914976458 35.5 30.0 39.0 12.5 40.0 60-61 31.520268616749505 35.0 30.0 39.0 10.5 40.0 62-63 31.150355159972648 35.0 29.0 39.0 8.0 40.0 64-65 30.72314343432226 35.0 28.5 38.0 7.5 40.0 66-67 29.08399801917418 33.5 25.0 37.5 4.5 39.5 68-69 25.534917295281915 30.0 16.0 36.0 2.0 39.0 70-71 24.954211743991365 29.5 15.0 35.0 2.0 38.0 72-73 26.719786773009417 31.0 21.5 35.0 2.0 38.0 74-75 27.285777618240367 32.0 23.5 35.0 2.0 38.0 76-77 27.268588714009102 31.5 25.0 34.5 2.0 37.0 78-79 28.071987559575433 33.0 26.0 35.0 2.0 37.0 80-81 27.91539568043725 33.0 26.0 35.0 2.0 37.0 82-83 27.50178536337411 33.0 26.0 35.0 2.0 36.5 84-85 27.13973416059886 32.5 26.0 35.0 2.0 36.0 86-87 26.681172460232826 32.0 25.0 35.0 2.0 35.5 88-89 26.14560772836485 32.0 24.0 34.5 2.0 35.0 90-91 25.663132010512 32.0 22.5 34.0 2.0 35.0 92-93 25.135294333423293 31.5 20.0 34.0 2.0 35.0 94-95 23.802539963684858 31.0 7.0 34.0 2.0 35.0 96-97 22.994543034488903 30.0 2.0 34.0 2.0 35.0 98-99 22.659424774471454 30.0 2.0 34.0 2.0 35.0 100-101 21.424724950806873 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3943577545930417 1101 2 0.3404640603641198 1101 3 0.3634444558163956 1101 4 0.26675866643984136 1101 5 0.42852982843228205 1101 6 0.5670499824510102 1101 7 0.5169173800283673 1101 8 0.6219796909863149 1101 9 0.6783631135440373 1101 10-11 0.88358300712774 1101 12-13 0.6143029382726723 1101 14-15 0.8039408096208618 1101 16-17 0.7846537023687503 1101 18-19 0.9312011990784157 1101 20-21 1.0045728730128616 1101 22-23 0.9998591973069395 1101 24-25 0.899518802036134 1101 26-27 0.885960251931337 1101 28-29 0.947124769688692 1101 30-31 0.9484146860449769 1101 32-33 0.9279509736398595 1101 34-35 1.1031858635102978 1101 36-37 1.1501090219423347 1101 38-39 0.9899739487927164 1101 40-41 1.0801448766852175 1101 42-43 1.1834435243418113 1101 44-45 0.9226912460884265 1101 46-47 0.9297991985329261 1101 48-49 0.8027720746104023 1101 50-51 0.8722315901161721 1101 52-53 1.0362026920901144 1101 54-55 0.9566906395740062 1101 56-57 0.836427426034767 1101 58-59 0.8952718050226061 1101 60-61 0.709835312944513 1101 62-63 0.7140913383857175 1101 64-65 0.4135725956399021 1101 66-67 -2.217034701658765 1101 68-69 -7.547167242066241 1101 70-71 -11.245956610759968 1101 72-73 -10.878327148941576 1101 74-75 -9.153592141995166 1101 76-77 -4.057360148463395 1101 78-79 -1.1147857501788216 1101 80-81 -0.4231408430865571 1101 82-83 -0.2192442314185037 1101 84-85 -0.3400169845634551 1101 86-87 -0.026464868681710385 1101 88-89 0.03545585815269803 1101 90-91 -0.13347147086281197 1101 92-93 -0.669654518146082 1101 94-95 -4.010679751239255 1101 96-97 -1.3397041452794625 1101 98-99 -0.5132523054422364 1101 100-101 -0.2456419576467539 1102 1 0.39732511364348966 1102 2 0.353322616249379 1102 3 0.45543258638019424 1102 4 0.27714442311640397 1102 5 0.538816673140488 1102 6 0.5433111100474477 1102 7 0.45163548091857564 1102 8 0.5126819659615904 1102 9 0.7411722134451253 1102 10-11 0.7804672801247747 1102 12-13 0.7995155990046214 1102 14-15 0.7082434802440076 1102 16-17 0.8093816944557943 1102 18-19 0.7912407638657584 1102 20-21 0.944731132162218 1102 22-23 0.7995624614018979 1102 24-25 0.8062942718679764 1102 26-27 0.7979286001014714 1102 28-29 0.8440090426857196 1102 30-31 0.5277915406443867 1102 32-33 0.6811656126111743 1102 34-35 0.7735617289900247 1102 36-37 0.981958675750441 1102 38-39 0.9902212287135868 1102 40-41 0.979501948890956 1102 42-43 1.1740468873487302 1102 44-45 0.9298623637936672 1102 46-47 1.0343986050611207 1102 48-49 0.9180045177360228 1102 50-51 0.9234185337363527 1102 52-53 0.8235419601415472 1102 54-55 0.7962059709291012 1102 56-57 0.7338062588735426 1102 58-59 0.7511076111551453 1102 60-61 0.620319981589418 1102 62-63 0.5548430693451571 1102 64-65 0.759764484858497 1102 66-67 -2.168567837168162 1102 68-69 -7.478423424064264 1102 70-71 -10.822613386229799 1102 72-73 -8.504934468427237 1102 74-75 -6.914472458513462 1102 76-77 -3.349892294853113 1102 78-79 -0.634073584006714 1102 80-81 -0.032191288190414724 1102 82-83 0.1855529990463829 1102 84-85 0.2851066553969801 1102 86-87 0.3545934893796172 1102 88-89 0.4845161944533949 1102 90-91 0.35861557166933267 1102 92-93 -0.04972375652392458 1102 94-95 -3.6385234703292646 1102 96-97 -0.4752135419164567 1102 98-99 0.1294282088999985 1102 100-101 0.3495608118883631 1103 1 0.351791695528469 1103 2 0.22472716619992283 1103 3 0.3943756413469863 1103 4 0.17997613146739866 1103 5 0.32607398556100975 1103 6 0.34125091504956373 1103 7 0.456244778643601 1103 8 0.35138339311998834 1103 9 0.6130783883780069 1103 10-11 0.5325232360383012 1103 12-13 0.4683837634810928 1103 14-15 0.6435677701973788 1103 16-17 0.7246282678626059 1103 18-19 0.6069041805677529 1103 20-21 0.6127196866344562 1103 22-23 0.7586637755180448 1103 24-25 0.837664202629604 1103 26-27 0.5203957471976963 1103 28-29 0.682873674591896 1103 30-31 0.7498157034252273 1103 32-33 0.7091541670834118 1103 34-35 0.9133285793503418 1103 36-37 0.8430262184945505 1103 38-39 0.8777879942922269 1103 40-41 1.02026286453318 1103 42-43 0.8607559448589797 1103 44-45 0.8633313478264526 1103 46-47 0.8208589696223711 1103 48-49 0.8506864451063763 1103 50-51 0.666515184859712 1103 52-53 0.7489580969226708 1103 54-55 0.8233926319436549 1103 56-57 0.8469989959090114 1103 58-59 0.7651255566122579 1103 60-61 0.6396078154173139 1103 62-63 0.46940997622660774 1103 64-65 0.4445313352188158 1103 66-67 -2.5648565188129275 1103 68-69 -7.204165970340934 1103 70-71 -11.284092120156604 1103 72-73 -8.673854914944123 1103 74-75 -6.890841682761025 1103 76-77 -3.3798216434471477 1103 78-79 -0.699947541898478 1103 80-81 -0.1660198878795498 1103 82-83 0.07165728052158116 1103 84-85 -0.04605909280141063 1103 86-87 0.06117678738725019 1103 88-89 -0.05113097242522713 1103 90-91 0.016889557821659906 1103 92-93 0.1655100996298131 1103 94-95 1.0139118836342789 1103 96-97 1.0744374401380874 1103 98-99 0.5970465500049862 1103 100-101 0.4841863594367588 1104 1 0.32475503913929415 1104 2 0.1356863988138315 1104 3 0.23195700482763115 1104 4 0.23729713180998147 1104 5 0.36501197802417806 1104 6 0.4034502984000312 1104 7 0.48881072588580565 1104 8 0.2251655102834036 1104 9 0.41110510985796367 1104 10-11 0.47251981020753675 1104 12-13 0.4035293612885624 1104 14-15 0.4623995619068708 1104 16-17 0.5075162431966262 1104 18-19 0.518140905473544 1104 20-21 0.5964521292517873 1104 22-23 0.46013003108502204 1104 24-25 0.5598344664185717 1104 26-27 0.8031638184549763 1104 28-29 0.7462121466713754 1104 30-31 0.7284881940041856 1104 32-33 0.5254525569429518 1104 34-35 0.6715642765069063 1104 36-37 0.5924010043801218 1104 38-39 0.6262052604792743 1104 40-41 0.6834506000590395 1104 42-43 0.9514102785348939 1104 44-45 0.6915019541985004 1104 46-47 0.6692796616401822 1104 48-49 0.8300184081074065 1104 50-51 0.7092986723554233 1104 52-53 0.6475569321402119 1104 54-55 0.6606485415017218 1104 56-57 0.5423895406160995 1104 58-59 0.6311978416413773 1104 60-61 0.4071138106211478 1104 62-63 0.5328803427904987 1104 64-65 0.345416912471304 1104 66-67 -2.806601979587164 1104 68-69 -7.637632911074061 1104 70-71 -8.733048954688979 1104 72-73 -7.875841896787218 1104 74-75 -6.480528219246121 1104 76-77 -3.3578038291271746 1104 78-79 -0.6438050273386935 1104 80-81 0.056568327262620954 1104 82-83 0.16185255287512845 1104 84-85 0.16636639537947318 1104 86-87 0.20888833243692417 1104 88-89 0.4247940018810432 1104 90-91 0.3424171357593089 1104 92-93 0.405705371983359 1104 94-95 1.2790728976801873 1104 96-97 1.2115528906348345 1104 98-99 0.850936580837466 1104 100-101 0.7724991377855055 1105 1 0.4912280414954253 1105 2 0.479722307113434 1105 3 0.5466594119981032 1105 4 0.41012190406215154 1105 5 0.4788047921693632 1105 6 0.46803419979203653 1105 7 0.6450300070206652 1105 8 0.6305573484163958 1105 9 0.9119510803119226 1105 10-11 0.8370907773405349 1105 12-13 0.8652156390884755 1105 14-15 0.9972315214121465 1105 16-17 1.0366788023621183 1105 18-19 0.9913551037941843 1105 20-21 1.143978190035817 1105 22-23 1.2065549319806266 1105 24-25 1.3134805291602092 1105 26-27 1.2735055625524083 1105 28-29 1.17736832959676 1105 30-31 1.0946602126574874 1105 32-33 1.1632115043040088 1105 34-35 1.2157969952881515 1105 36-37 1.074148667474141 1105 38-39 0.9473992673728873 1105 40-41 0.9769635448052867 1105 42-43 1.1496966640037698 1105 44-45 0.9864411631811691 1105 46-47 0.951510087212263 1105 48-49 0.7642627327643012 1105 50-51 0.9875404521094637 1105 52-53 1.2240892922579505 1105 54-55 1.3022070148396736 1105 56-57 1.4354295109721775 1105 58-59 1.4747611251253794 1105 60-61 1.462494112793408 1105 62-63 1.4613633289962884 1105 64-65 1.3550609756464205 1105 66-67 2.1219219068430526 1105 68-69 -4.859987155958038 1105 70-71 -4.864369088086633 1105 72-73 -1.3017460714067042 1105 74-75 1.331369201592718 1105 76-77 1.5204407472234038 1105 78-79 1.436233216119014 1105 80-81 1.4363464872419272 1105 82-83 1.4366524214350882 1105 84-85 1.305320887762246 1105 86-87 1.4817501595932718 1105 88-89 1.472362710762333 1105 90-91 1.4730751749256434 1105 92-93 1.4877452794160142 1105 94-95 2.3786397747907415 1105 96-97 2.179869898835854 1105 98-99 1.808130126292646 1105 100-101 1.7646868453454054 1106 1 0.21051705334645376 1106 2 0.24042974969078656 1106 3 0.254056980801483 1106 4 0.02505399626171112 1106 5 0.2810528315417429 1106 6 0.13958199795531812 1106 7 0.3390614138042807 1106 8 0.25836601981906426 1106 9 0.41061552043309746 1106 10-11 0.46272847503371395 1106 12-13 0.4861072309399077 1106 14-15 0.48694431174026676 1106 16-17 0.42709698691922426 1106 18-19 0.36606117039582386 1106 20-21 0.5027381401406217 1106 22-23 0.41996137206138684 1106 24-25 0.3635589726359072 1106 26-27 0.601519160646248 1106 28-29 0.6820554191233583 1106 30-31 0.6697112835854568 1106 32-33 0.6750579388000375 1106 34-35 0.7105011421372751 1106 36-37 0.7168402431592327 1106 38-39 0.7031755179217143 1106 40-41 0.8212098444593678 1106 42-43 0.8257928518713058 1106 44-45 0.8368577678462543 1106 46-47 0.6470462952142313 1106 48-49 0.5967004877744841 1106 50-51 0.6850168501761189 1106 52-53 0.8737767124596232 1106 54-55 0.787291272685394 1106 56-57 0.8352873206907176 1106 58-59 0.8465473767580924 1106 60-61 0.8691289244280256 1106 62-63 0.9300157913314777 1106 64-65 0.9339439736201136 1106 66-67 1.6438116781444982 1106 68-69 -5.23378294756661 1106 70-71 -5.993096530994663 1106 72-73 -2.0632441488985727 1106 74-75 0.6533909214337719 1106 76-77 0.933867257444021 1106 78-79 0.6848062613241339 1106 80-81 0.6977311248128615 1106 82-83 0.7861842406440083 1106 84-85 0.9747659139498701 1106 86-87 0.9625896058227532 1106 88-89 1.0533946374406469 1106 90-91 0.9945171570655127 1106 92-93 0.9580143754422181 1106 94-95 1.83565821543732 1106 96-97 1.8464389704288457 1106 98-99 1.5086322012787328 1106 100-101 1.2441071443858966 1107 1 0.0374259646876034 1107 2 0.01626587535814039 1107 3 0.013476144899446041 1107 4 -0.007987355231001914 1107 5 0.17652902223526468 1107 6 0.2216592753999862 1107 7 0.2330385980545131 1107 8 0.13249511738477793 1107 9 0.1327528037742951 1107 10-11 0.23338893238926772 1107 12-13 0.266470953934288 1107 14-15 0.2021759879895697 1107 16-17 -0.01081929600118059 1107 18-19 0.22793841753632194 1107 20-21 0.18781648827157227 1107 22-23 0.30621430321343013 1107 24-25 0.4161134220083298 1107 26-27 0.36509465041905287 1107 28-29 0.36319753470088756 1107 30-31 0.3556078213346723 1107 32-33 0.35729458694039096 1107 34-35 0.5444639866759999 1107 36-37 0.5375360021279434 1107 38-39 0.24856056792815195 1107 40-41 0.2559186323931968 1107 42-43 0.46266852447314477 1107 44-45 0.34338400239866473 1107 46-47 0.4667227115459909 1107 48-49 0.37472659134353137 1107 50-51 0.6091806788699685 1107 52-53 0.6397605618004647 1107 54-55 0.48184035676028003 1107 56-57 0.5874074712257382 1107 58-59 0.6437207832108633 1107 60-61 0.684714849056455 1107 62-63 0.8391101440774271 1107 64-65 0.86951271067581 1107 66-67 1.5123641705250996 1107 68-69 -5.318123110898753 1107 70-71 -5.495332261795102 1107 72-73 -1.716528111697471 1107 74-75 0.7002287685967268 1107 76-77 0.694773790090828 1107 78-79 0.5727760101007888 1107 80-81 0.7009460361047211 1107 82-83 0.8382485292856963 1107 84-85 0.8751321881532697 1107 86-87 0.8399431988286956 1107 88-89 0.5140968908108441 1107 90-91 0.6172444588356605 1107 92-93 0.6258483305281608 1107 94-95 1.3571747426165963 1107 96-97 1.3864665955955395 1107 98-99 1.1231196455879804 1107 100-101 0.958299739216482 1108 1 0.0437024270341837 1108 2 -0.08047694823273588 1108 3 -0.016190552343605447 1108 4 -0.16533497658237906 1108 5 -0.044198320082564635 1108 6 0.0862791558540863 1108 7 0.08380504865323957 1108 8 0.044592283233413355 1108 9 0.17077779600990795 1108 10-11 0.13961787717344265 1108 12-13 0.2522231352953952 1108 14-15 0.37882561103862855 1108 16-17 -0.054482720795157036 1108 18-19 0.22217717501143142 1108 20-21 0.12530842704865108 1108 22-23 0.14587000227104596 1108 24-25 0.3475597346128865 1108 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2316 100-101 0.0655729140586061 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 22.0 4 59.0 5 195.0 6 515.0 7 1117.0 8 2076.0 9 3469.0 10 5536.0 11 8207.0 12 10314.0 13 13002.0 14 16035.0 15 20605.0 16 24829.0 17 28479.0 18 24864.0 19 20067.0 20 19138.0 21 19690.0 22 20837.0 23 24329.0 24 29000.0 25 35556.0 26 44192.0 27 55203.0 28 66830.0 29 79667.0 30 94048.0 31 110275.0 32 127561.0 33 148174.0 34 173490.0 35 200227.0 36 207290.0 37 184349.0 38 83850.0 39 5190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1601849410672 22.785846483818705 16.22301741593028 19.830951159183815 2 15.000214893030442 23.540063922814618 39.75112242298706 21.708598761167885 3 18.48844125253171 29.075798029130716 31.71747554754375 20.718285170793823 4 11.71150162841699 19.973012558330865 39.22150401274436 29.093981800507784 5 11.241198285588219 40.77177875724535 34.70656759732389 13.280455359842541 6 26.47829607057609 40.22103500769011 18.509978803067657 14.790690118666138 7 25.222882206367466 36.38399723313219 21.84599341296812 16.54712714753222 8 25.066564655883916 35.32692796449186 20.276215155413603 19.33029222421062 9 25.98466170062805 18.259388616539894 22.300535294595434 33.45541438823662 10-11 23.152237992553562 29.080330871275144 27.42303993879353 20.344391197377764 12-13 25.455183815919447 27.381327310504926 27.993758826596515 19.16973004697911 14-15 22.765217117898437 28.035838274480625 28.241440657759938 20.957503949861 16-17 18.82270267664281 31.611089742313258 29.261022718816783 20.30518486222715 18-19 18.51735496826448 29.68890179177811 33.03588272531379 18.757860514643614 20-21 19.83386731813861 28.468909926877096 32.78459713230771 18.91262562267659 22-23 19.456320956665504 28.4143960970395 32.53917764286968 19.59010530342531 24-25 19.329846800416078 29.05588496537485 32.979465963071746 18.634802271137325 26-27 19.434392513275668 28.46360173234127 33.53711147368225 18.56489428070081 28-29 18.42601662832707 28.580061626385188 34.13110098854843 18.862820756739303 30-31 18.740586747607974 29.129856504563957 33.592799340115945 18.53675740771213 32-33 18.707002947920078 29.175997379626594 32.93895840157222 19.1780412708811 34-35 19.207405431439334 28.759669893764155 32.92836954624805 19.104555128548466 36-37 19.60722239518486 28.407946093010068 33.11911611924648 18.865715392558595 38-39 19.203598664926787 28.929095546914446 33.2160969436266 18.651208844532167 40-41 18.654061770879952 29.01876148687892 33.81710921264085 18.510067529600274 42-43 18.534893151150587 29.363276645590837 32.82696608230328 19.2748641209553 44-45 18.757060190922513 29.39045849047053 32.30533543889963 19.54714587970733 46-47 19.22011956397155 29.528387722359582 31.462614161613583 19.788878552055284 48-49 19.06738939533986 29.67422653588586 31.463011476718698 19.79537259205558 50-51 19.443833178280098 29.636719054289788 31.3586099396512 19.56083782777891 52-53 19.174573472988197 30.147850110866603 31.242487397707986 19.43508901843721 54-55 18.859484907692615 29.898196012790784 31.4467293620654 19.795589717451197 56-57 19.39935341517257 30.442984523168594 30.4106133434265 19.747048718232342 58-59 19.233806056060583 29.657605558101867 31.01516019873275 20.093428187104802 60-61 19.344090983294908 29.92423736217203 31.13573730937166 19.595934345161403 62-63 19.88472244930471 29.574236059580855 31.239440664394447 19.30160082671999 64-65 19.58960757741073 29.661675570354856 31.215864391431076 19.532852460803333 66-67 19.12346750601 29.78581326996337 31.105658384702593 19.98506083932404 68-69 19.300234765776967 29.856586607548685 31.116699640575153 19.726478986099195 70-71 19.265735171511032 29.837124922879116 31.235504313747054 19.661635591862805 72-73 19.590038507457667 29.55163614656375 30.461091072659706 20.397234273318873 74-75 18.976129418744485 30.341203602510515 30.66332302022903 20.01934395851597 76-77 19.333855164781774 29.94344671331354 30.832377064538253 19.89032105736643 78-79 19.0351398737456 29.652463679783075 31.028757714025474 20.283638732445848 80-81 18.95928391421937 30.576995938190908 30.401653231236992 20.062066916352723 82-83 19.075850431930075 30.443065668568014 30.21553271375757 20.265551185744343 84-85 18.866082026101836 29.911858491480615 30.19999528374806 21.022064198669494 86-87 18.992746905735977 30.011858790780643 30.613235207036247 20.38215909644713 88-89 19.612012779016965 29.38676072671554 30.500195332509385 20.501031161758114 90-91 18.95375874304526 29.627021047242184 31.03083431722889 20.38838589248367 92-93 19.023342426242497 30.175654462813966 30.62622116914147 20.17478194180206 94-95 18.556109514823767 29.838146791795054 30.593108135148324 21.01263555823286 96-97 18.712139853447898 29.559584750661056 30.253242641866485 21.47503275402456 98-99 18.947661642104645 29.451915512725975 30.020329900991964 21.580092944177416 100-101 19.00378822717414 29.404210416537286 30.4291568502137 21.16284450607487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10402.0 1 6785.0 2 3222.0 3 3407.0 4 3148.0 5 3123.0 6 5111.0 7 9369.5 8 12754.0 9 13765.0 10 12172.5 11 9243.0 12 7674.0 13 6828.5 14 6171.5 15 5555.5 16 5117.5 17 4976.5 18 4948.0 19 5034.0 20 5533.0 21 6217.5 22 6893.5 23 7907.0 24 8982.0 25 10831.0 26 14144.0 27 17513.5 28 19707.0 29 22876.5 30 27369.0 31 32532.0 32 40485.0 33 50124.5 34 59334.0 35 67282.0 36 76955.0 37 84960.0 38 84889.0 39 81325.0 40 76966.5 41 71989.5 42 67191.5 43 65575.5 44 65378.0 45 63726.0 46 61794.0 47 63883.5 48 78028.0 49 73496.0 50 52437.0 51 42307.0 52 35599.0 53 31669.5 54 28878.0 55 26270.5 56 23676.0 57 21348.0 58 19465.5 59 17267.5 60 14732.5 61 12204.5 62 9570.5 63 6997.0 64 4928.0 65 3508.0 66 2534.5 67 1915.0 68 1373.5 69 917.0 70 657.0 71 536.5 72 466.0 73 424.5 74 353.0 75 260.0 76 188.0 77 117.5 78 68.0 79 42.5 80 30.5 81 26.0 82 17.0 83 8.0 84 4.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023266842914748505 2 0.01933663296293288 3 0.0 4 0.0 5 1.0480559871508335E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.7162519421787517E-4 18-19 0.0012052643852234585 20-21 5.240279935754168E-5 22-23 0.0 24-25 0.0 26-27 5.502293932541876E-4 28-29 0.01813136857770942 30-31 0.004244626747960876 32-33 0.008908475890782086 34-35 0.009537309483072586 36-37 0.10346932733146605 38-39 0.10650868969420346 40-41 0.12136488331206652 42-43 0.10501520991251354 44-45 0.10058717336680126 46-47 0.1022640629462426 48-49 0.10485800151444091 50-51 0.10273568814046045 52-53 0.07855179623695499 54-55 0.06796643076673156 56-57 0.03846365472843559 58-59 0.016009055203728983 60-61 0.008620260494315605 62-63 0.0016244867800837923 64-65 0.005083071537681543 66-67 0.4150563723114089 68-69 0.792932958478642 70-71 0.8795285844169795 72-73 0.27705360020332287 74-75 0.14623001160722007 76-77 0.014227360025572566 78-79 0.008567857694958065 80-81 0.0016768895794413337 82-83 0.0 84-85 0.0 86-87 3.144167961452501E-4 88-89 5.502293932541876E-4 90-91 0.0033537791588826673 92-93 0.0016506881797625629 94-95 0.22134942448625605 96-97 0.22693032261783425 98-99 0.014043950227821171 100-101 0.007677010105879856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1908295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.916029326943175 #Duplication Level Percentage of deduplicated Percentage of total 1 72.1878372794999 25.205126434996206 2 13.566074931596447 9.473469403262603 3 5.1878498684448475 5.434173544511957 4 2.5769349198720666 3.599053409435081 5 1.5023183639173756 2.6227496026472186 6 0.9189808317470776 1.9252297003307755 7 0.6540164929142586 1.5984961332829142 8 0.49518130220509315 1.3831811895957553 9 0.3541490636741307 1.112893118401987 >10 2.0464298354179373 13.73958289992194 >50 0.26447444075047777 6.526834775085081 >100 0.22309147803281157 15.127999024920848 >500 0.015311616167449074 3.7051362636143135 >1k 0.006430878790328611 4.310433977694904 >5k 3.0623232334898147E-4 0.7483622506359396 >10k+ 6.124646466979629E-4 3.487278271662502 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20471 1.0727377056482357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18916 0.9912513526472584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13339 0.6990009406302485 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12503 0.6551922003673436 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7591 0.3977896499230989 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6407 0.33574473548376954 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4752 0.24901810254703807 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4744 0.24859888015217774 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4643 0.24330619741706602 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4614 0.24178651623569733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3976 0.20835353024558573 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3053 0.15998574643857474 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2827 0.14814271378377034 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2799 0.14667543540175915 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2732 0.14316444784480387 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2574 0.13488480554631227 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2429 0.12728639963946875 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2209 0.11575778378080959 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.11072711504248557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.620139967877084E-5 0.0 0.0 0.0 12-13 0.0 5.240279935754168E-5 0.0 0.0 0.0 14-15 0.0 5.240279935754168E-5 0.0 5.240279935754168E-5 0.0 16-17 0.0 5.240279935754168E-5 0.0 1.0480559871508335E-4 0.0 18-19 0.0 5.240279935754168E-5 0.0 1.5720839807262504E-4 0.0 20-21 0.0 5.240279935754168E-5 0.0 3.668195955027917E-4 0.0 22-23 0.0 5.240279935754168E-5 0.0 5.240279935754169E-4 0.0 24-25 0.0 5.240279935754168E-5 0.0 0.0015720839807262504 0.0 26-27 0.0 5.240279935754168E-5 0.0 0.003406181958240209 0.0 28-29 0.0 5.240279935754168E-5 0.0 0.0062359331235474595 0.0 30-31 0.0 5.240279935754168E-5 0.0 0.013781936231033461 0.0 32-33 5.240279935754168E-5 5.240279935754168E-5 0.0 0.02554636468680157 0.0 34-35 5.240279935754168E-5 5.240279935754168E-5 0.0 0.04380874026290484 0.0 36-37 5.240279935754168E-5 5.240279935754168E-5 0.0 0.07525041987742985 0.0 38-39 5.240279935754168E-5 5.240279935754168E-5 0.0 0.11494554039076768 0.0 40-41 5.240279935754168E-5 5.240279935754168E-5 0.0 0.15985473944018092 0.0 42-43 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.20484254268863042 5.240279935754168E-5 44-45 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.2574549532436023 5.240279935754168E-5 46-47 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.3213601670601244 5.240279935754168E-5 48-49 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.39632237154108774 5.240279935754168E-5 50-51 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.47455975098189745 5.240279935754168E-5 52-53 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.5488931218705704 5.240279935754168E-5 54-55 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.6339690666275393 5.240279935754168E-5 56-57 5.240279935754168E-5 1.0480559871508335E-4 0.0 0.7306784328418824 5.240279935754168E-5 58-59 7.860419903631252E-5 1.0480559871508335E-4 0.0 0.8259991248732508 5.240279935754168E-5 60-61 1.0480559871508335E-4 1.0480559871508335E-4 0.0 0.9317479739767698 5.240279935754168E-5 62-63 1.0480559871508335E-4 1.310069983938542E-4 0.0 1.0312608899567415 5.240279935754168E-5 64-65 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.1309834171341433 5.240279935754168E-5 66-67 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.2388021768122854 5.240279935754168E-5 68-69 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.3668484170424384 5.240279935754168E-5 70-71 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.5399610647200772 5.240279935754168E-5 72-73 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.728191920012367 5.240279935754168E-5 74-75 1.0480559871508335E-4 1.5720839807262504E-4 0.0 1.933768101892003 5.240279935754168E-5 76-77 1.0480559871508335E-4 1.5720839807262504E-4 0.0 2.153781254994642 5.240279935754168E-5 78-79 1.0480559871508335E-4 1.5720839807262504E-4 0.0 2.417314932963719 5.240279935754168E-5 80-81 1.0480559871508335E-4 1.5720839807262504E-4 0.0 2.700709271889304 5.240279935754168E-5 82-83 1.0480559871508335E-4 1.5720839807262504E-4 0.0 2.9843918262113562 5.240279935754168E-5 84-85 1.0480559871508335E-4 1.8340979775139586E-4 0.0 3.2747819388511736 5.240279935754168E-5 86-87 1.0480559871508335E-4 2.096111974301667E-4 0.0 3.5781417443319823 5.240279935754168E-5 88-89 1.0480559871508335E-4 2.096111974301667E-4 0.0 3.8964101462300116 5.240279935754168E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 470 0.0 36.28564 9 TACGCGG 45 2.1742613E-5 32.15572 64-65 CTAACGC 105 7.0353184E-5 27.070238 3 TACCTGG 930 0.0 27.00384 2 TAGGCAT 750 0.0 25.265558 5 GTACATG 14995 0.0 24.275108 1 TACATGG 15230 0.0 23.24092 2 TTAGGCA 820 0.0 23.108742 4 ACATGGG 15050 0.0 22.443117 3 CATGGGG 3010 0.0 22.1913 4 GAGTACT 13900 0.0 22.187468 12-13 GTATAGG 1030 0.0 22.087696 1 GTATCGA 110 4.8961738E-8 21.884361 62-63 CCTTTAG 795 0.0 21.462574 1 GGACAAT 895 0.0 21.172255 6 GTACTTT 14805 0.0 20.86319 14-15 AGTACTT 14550 0.0 20.82184 12-13 CATGGGA 11540 0.0 20.566582 4 CTTTAGG 925 0.0 20.49042 2 CTTACTA 1205 0.0 20.04995 9 >>END_MODULE