FastQCFastQC Report
Fri 27 May 2016
SRR939492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896392
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148780.7845424363739143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111260.5866930465853052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84740.4468485418626529No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81710.4308708326126666No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52630.2775270091837553No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43140.2274846128859434No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.15708777510135036No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.15149821344953995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.14453762724162514No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25830.13620601647760588No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.11474420900320188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG4500.025.8784289
GTACATG136300.025.7471891
TACATGG141750.024.23772
GAGTACT113100.023.70691112-13
ACATGGG141700.023.697733
GTACTTT125600.021.81226714-15
CATGGGA105100.021.5398964
TACCTGG9100.021.512752
AGTACTT121600.021.35762212-13
ATGGGAT40550.020.8332375
ACTTTTT151650.020.28745716-17
GTATCAA291500.019.7361981
CATGGGT16200.019.7111764
CATATAG7050.019.1068923
CATGGGG29550.019.0316854
ATGGGCG3658.54925E-1118.6223095
GGACAAT8800.018.1937946
TCGACCG650.00778268318.18008818-19
CAACGCG4052.0008883E-1117.9818615
TACTTTT150350.017.5905714-15
Produced by FastQC (version 0.11.5)