FastQCFastQC Report
Fri 27 May 2016
SRR939492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896392
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197161.0396584672367317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180480.9517019687912626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130590.6886234491603002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122750.6472817856223819No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA71660.37787546034786057No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA63040.33242072314162896No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46460.2449915418331231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.24050934616893552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45010.23734544334715607No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT43740.23064851570772288No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37030.1952655358174892No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29580.15598040911372754No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28440.14996899375234657No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27180.1433247978266097No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT26240.1383680167391552No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25100.13235660137777422No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.12977274740665432No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.1126349404553489No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.10762542765419808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3750.030.3468749
TAGGACG1351.3530538E-524.6067034
GTACGCG601.5965232E-423.9954964-65
AGGCTTA10100.023.0042826
GTACATG147150.022.9631711
ATGGGCG2903.6379788E-1222.8909635
TACCTGG8750.022.7787782
CTACACG7550.022.6279264
GAGTACT133300.022.01303712-13
TAGGACA12800.021.8741534
GTATAGG10650.021.8352721
TACGCGG550.002711805521.81408164-65
TACATGG152800.021.7712752
ACATGGG148550.021.33433
GGACAAT8750.021.134436
CGGAGGT7450.021.00359
GTACTTT141400.020.7185114-15
TTAGGCA6650.020.6950044
CATGGGT12550.020.4192244
AGTACTT140550.020.33789312-13
Produced by FastQC (version 0.11.5)