##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150711 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.34227621009967 31.0 27.0 31.0 10.0 33.0 2 27.295701527142786 31.0 26.0 31.0 16.0 34.0 3 27.79647452748779 31.0 27.0 31.0 16.0 34.0 4 31.543771633364067 35.0 32.0 37.0 19.0 37.0 5 31.862072231863603 35.0 32.0 37.0 19.0 37.0 6 32.04181762406026 35.0 32.0 37.0 19.0 37.0 7 31.542921724047133 35.0 32.0 37.0 17.0 37.0 8 31.5350813540498 35.0 32.0 37.0 17.0 37.0 9 32.47829298581486 37.0 31.0 39.0 17.0 39.0 10-11 32.11571367615326 35.0 30.0 39.0 16.5 39.0 12-13 31.915653452517617 35.0 30.0 39.0 17.0 39.0 14-15 32.48189293402079 36.0 30.5 39.0 17.0 41.0 16-17 32.57056332997599 36.0 30.5 39.0 17.0 40.5 18-19 32.96104017429224 37.0 31.0 39.5 17.5 41.0 20-21 32.852724967433176 37.0 31.0 39.0 18.0 41.0 22-23 32.65322917743899 37.0 31.0 39.0 17.0 41.0 24-25 32.4482650291863 36.5 30.5 39.0 16.0 41.0 26-27 32.25502146064477 36.0 30.0 39.0 16.0 41.0 28-29 32.23566733958396 36.0 30.0 39.0 16.0 41.0 30-31 31.821466467253728 36.0 30.0 39.0 13.0 40.0 32-33 31.160422556141377 35.0 28.5 39.0 9.5 40.0 34-35 30.68343180868176 35.0 27.0 39.0 9.0 40.0 36-37 30.278908431395894 35.0 26.5 38.0 9.0 40.0 38-39 29.739685290224912 35.0 25.0 38.0 8.0 40.0 40-41 29.582422954156172 35.0 25.0 38.0 7.5 40.0 42-43 29.084297447404257 34.0 24.0 38.0 2.0 40.0 44-45 28.84433754435301 34.0 24.0 38.0 2.0 40.0 46-47 27.995171680812994 33.0 22.5 38.0 2.0 40.0 48-49 27.302356977555615 33.0 20.0 38.0 2.0 40.0 50-51 26.76682025286975 32.5 19.5 37.5 2.0 39.5 52-53 27.010389663434175 33.0 20.5 37.5 2.0 39.0 54-55 26.91988214243194 33.0 19.5 38.0 2.0 40.0 56-57 26.53472157648619 33.0 18.0 38.0 2.0 40.0 58-59 26.115735401851552 32.5 14.5 37.5 2.0 40.0 60-61 25.49774139640622 31.5 11.0 37.0 2.0 39.0 62-63 24.84125423325231 31.0 8.0 36.0 2.0 39.0 64-65 24.179978291682275 31.0 7.5 36.0 2.0 39.0 66-67 23.817207795875767 30.0 6.5 35.0 2.0 38.0 68-69 23.515524749480974 30.0 2.0 35.0 2.0 38.0 70-71 22.981854262277842 29.5 2.0 35.0 2.0 37.5 72-73 22.296733932325317 29.0 2.0 34.0 2.0 37.0 74-75 21.654975923581162 28.5 2.0 34.0 2.0 36.0 76-77 20.933485036642562 27.5 2.0 34.0 2.0 36.0 78-79 20.185971977325323 26.0 2.0 33.0 2.0 35.0 80-81 19.514930334375876 26.0 2.0 33.0 2.0 35.0 82-83 18.729458569527885 24.5 2.0 32.0 2.0 35.0 84-85 17.97063380814123 23.5 2.0 32.0 2.0 34.0 86-87 17.26188460873321 20.0 2.0 31.5 2.0 34.0 88-89 16.282222903926353 16.5 2.0 31.0 2.0 34.0 90-91 15.43821515567332 7.5 2.0 31.0 2.0 34.0 92-93 14.664675144323814 2.0 2.0 30.0 2.0 34.0 94-95 13.85174861455222 2.0 2.0 29.5 2.0 34.0 96-97 12.808307211802095 2.0 2.0 29.0 2.0 33.5 98-99 11.814904002829556 2.0 2.0 28.0 2.0 33.0 100-101 10.502214717683241 2.0 2.0 24.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.5005856546685763 1101 2 3.5494518489684275 1101 3 2.929301397388553 1101 4 2.7547827822915245 1101 5 2.3429193216284077 1101 6 2.122702303026415 1101 7 2.6761668477926044 1101 8 2.5887814242294667 1101 9 2.700805024064927 1101 10-11 2.70506250643308 1101 12-13 2.77020397270584 1101 14-15 2.6380054013507177 1101 16-17 2.3334746714972354 1101 18-19 2.7304879051180038 1101 20-21 2.6950992792551958 1101 22-23 2.7596515535351713 1101 24-25 2.871916577558558 1101 26-27 2.817918523415642 1101 28-29 2.688632663081897 1101 30-31 2.762300435302233 1101 32-33 2.9928614940786638 1101 34-35 2.9955395001552034 1101 36-37 3.0967564263733784 1101 38-39 3.2603596572455125 1101 40-41 3.1820124256977103 1101 42-43 2.726575279305795 1101 44-45 2.6743044915154712 1101 46-47 2.7481956796058142 1101 48-49 2.791456536766095 1101 50-51 2.9455703643760494 1101 52-53 2.3924776471561415 1101 54-55 2.465499315856963 1101 56-57 2.418075107808253 1101 58-59 2.2939028291741934 1101 60-61 2.2805539610128314 1101 62-63 2.1891139023641024 1101 64-65 2.0746847247336895 1101 66-67 2.2582713382709123 1101 68-69 1.9449707840981496 1101 70-71 1.8771456295551374 1101 72-73 1.778457431333436 1101 74-75 1.8113260442260426 1101 76-77 1.7246158804725802 1101 78-79 1.51805073602495 1101 80-81 1.7162581422522614 1101 82-83 1.5541628417291946 1101 84-85 1.6264579464201638 1101 86-87 1.4273508090454463 1101 88-89 1.3290663097447464 1101 90-91 1.3536996008239743 1101 92-93 1.2166691196088077 1101 94-95 0.6288083233239536 1101 96-97 0.4834682939246999 1101 98-99 0.3287806819692971 1101 100-101 -0.15904797362459 1102 1 3.7417181980536434 1102 2 3.761378362649804 1102 3 3.1517746101352273 1102 4 2.967441184342203 1102 5 2.414946728866944 1102 6 2.218625552749785 1102 7 2.8182101855927932 1102 8 2.843110199883789 1102 9 3.0379212865827867 1102 10-11 3.0209053526934326 1102 12-13 3.3753668786849467 1102 14-15 3.34665231838391 1102 16-17 2.8727356519079237 1102 18-19 2.7280931079893875 1102 20-21 2.8265027811985632 1102 22-23 2.851118833329217 1102 24-25 3.2249720898665117 1102 26-27 3.0602034608332787 1102 28-29 2.9227764718484295 1102 30-31 3.1417122695131816 1102 32-33 3.2610690417730446 1102 34-35 3.7711879129374637 1102 36-37 3.426574309193388 1102 38-39 3.242685908475746 1102 40-41 3.244045351638672 1102 42-43 3.261168299328812 1102 44-45 3.131027855783195 1102 46-47 3.202187494688971 1102 48-49 3.148832484120181 1102 50-51 2.873026882005316 1102 52-53 2.61455085823469 1102 54-55 3.009502279553079 1102 56-57 2.984447582689125 1102 58-59 3.0949209394147914 1102 60-61 3.185505085048792 1102 62-63 2.6236682775691413 1102 64-65 2.4942103665434914 1102 66-67 2.418120484470652 1102 68-69 2.0790822035028533 1102 70-71 1.9374506594979195 1102 72-73 2.280987763345248 1102 74-75 2.161058519218116 1102 76-77 2.063355433860817 1102 78-79 2.048491523267156 1102 80-81 1.9882742201646586 1102 82-83 1.8752814701995817 1102 84-85 1.8143558629362389 1102 86-87 1.212331319824294 1102 88-89 0.8413429899602676 1102 90-91 0.8735856959180257 1102 92-93 0.8565101893196108 1102 94-95 0.6601074981597836 1102 96-97 0.5223028607413109 1102 98-99 0.26654410617818947 1102 100-101 0.3194593216273809 1103 1 3.914046284217683 1103 2 3.8947669293440867 1103 3 3.1915426300192387 1103 4 3.191964796604008 1103 5 2.533422288104724 1103 6 2.613820250347132 1103 7 3.1703228616491366 1103 8 2.9135327350950604 1103 9 3.452171493707887 1103 10-11 3.268212726347116 1103 12-13 3.223337052670029 1103 14-15 3.050049170082332 1103 16-17 2.6511117910959925 1103 18-19 2.772417880151778 1103 20-21 3.040255888075116 1103 22-23 3.1390227272811586 1103 24-25 3.351732653246799 1103 26-27 3.5055224334927644 1103 28-29 3.2367781288492594 1103 30-31 3.1843800408470706 1103 32-33 3.360489091483071 1103 34-35 3.514767034727022 1103 36-37 3.5868891393508058 1103 38-39 3.5442600592628217 1103 40-41 3.557218508225244 1103 42-43 3.212701025095182 1103 44-45 3.2238199021792155 1103 46-47 3.4097268484586465 1103 48-49 3.4048391121234935 1103 50-51 3.175429533206639 1103 52-53 3.1083371051278164 1103 54-55 3.336345692974785 1103 56-57 3.3199902504604566 1103 58-59 3.2175390007238214 1103 60-61 3.3346351596138284 1103 62-63 3.081000506235256 1103 64-65 3.105229422053018 1103 66-67 3.3704817106512657 1103 68-69 3.027715177819342 1103 70-71 2.9453214134332946 1103 72-73 2.8725370591198924 1103 74-75 3.0782084777930976 1103 76-77 2.7344407694034807 1103 78-79 2.7581021280724585 1103 80-81 2.7198400859475953 1103 82-83 2.0973195812186383 1103 84-85 2.3238836177001154 1103 86-87 2.0669294971233825 1103 88-89 2.437449038013291 1103 90-91 2.0778110480307053 1103 92-93 1.7736601478945904 1103 94-95 1.4944074153097429 1103 96-97 1.3251197621497628 1103 98-99 1.0722607590365154 1103 100-101 0.9590616414285389 1104 1 3.8364052970517513 1104 2 3.5028755344476217 1104 3 2.74574922552447 1104 4 2.9564453321937556 1104 5 2.538079008754309 1104 6 2.337533082340755 1104 7 2.939496226865355 1104 8 2.8163206824535223 1104 9 3.3405827187851713 1104 10-11 3.2691167881389447 1104 12-13 3.359027566641199 1104 14-15 3.256747987112128 1104 16-17 3.0538532648638324 1104 18-19 2.883952825951532 1104 20-21 3.186538034258966 1104 22-23 3.269920443254634 1104 24-25 3.1493976729429036 1104 26-27 3.1674521373389766 1104 28-29 2.925772513903361 1104 30-31 3.24936817503605 1104 32-33 3.3663156761154873 1104 34-35 3.6461856561264057 1104 36-37 3.652405534498026 1104 38-39 3.748542918264622 1104 40-41 3.5782763942586335 1104 42-43 3.511451654494703 1104 44-45 3.4350771431992797 1104 46-47 3.7076523208677123 1104 48-49 3.3590422304679883 1104 50-51 3.1499653489652673 1104 52-53 3.029361757528765 1104 54-55 3.1283857364927883 1104 56-57 3.4481931668621577 1104 58-59 3.461024506647366 1104 60-61 3.3746606231293867 1104 62-63 3.1808725868411933 1104 64-65 3.0806987217327837 1104 66-67 2.8152302952128068 1104 68-69 2.843733588048078 1104 70-71 2.78594541667508 1104 72-73 2.7654385023422066 1104 74-75 2.4774263172325277 1104 76-77 2.162568859980844 1104 78-79 2.497220788188688 1104 80-81 2.4715554295211675 1104 82-83 2.424178374389765 1104 84-85 2.2894797869388803 1104 86-87 1.892115909716587 1104 88-89 1.8573656797714477 1104 90-91 1.7320756185043944 1104 92-93 1.3546860173017947 1104 94-95 1.3691403593348088 1104 96-97 1.1411247986833555 1104 98-99 0.42654131107590487 1104 100-101 0.3645323520331445 1105 1 3.6906081333299454 1105 2 3.393359273364478 1105 3 1.8762230546935825 1105 4 2.6299249504362017 1105 5 2.54016353967679 1105 6 2.620862835003514 1105 7 2.8518839921978874 1105 8 2.9955767933033357 1105 9 3.335180744964717 1105 10-11 3.252257636235285 1105 12-13 3.658771306758066 1105 14-15 3.491955462778087 1105 16-17 3.3145773732284667 1105 18-19 2.8743972742669754 1105 20-21 3.3185012288364746 1105 22-23 3.431789011354148 1105 24-25 3.4283590075072112 1105 26-27 3.2340835779491997 1105 28-29 3.3918345311176736 1105 30-31 3.2620902008052646 1105 32-33 3.7313834282092166 1105 34-35 3.994993292825761 1105 36-37 3.902528922470456 1105 38-39 3.7427683611548694 1105 40-41 3.238823017492731 1105 42-43 3.5424544551597386 1105 44-45 3.469995455522792 1105 46-47 3.5139361073978073 1105 48-49 4.091073554561259 1105 50-51 3.791131834662906 1105 52-53 3.237348394853285 1105 54-55 3.450475764228468 1105 56-57 3.7587669838376314 1105 58-59 3.8462824020259774 1105 60-61 4.096076710598396 1105 62-63 3.8608922514968746 1105 64-65 3.610292480140103 1105 66-67 3.580511532692558 1105 68-69 3.472760632756138 1105 70-71 3.3354457679678937 1105 72-73 3.3057258850268596 1105 74-75 3.54977521777311 1105 76-77 3.607438146149974 1105 78-79 3.388142917313477 1105 80-81 3.1877785769112457 1105 82-83 3.274803443341863 1105 84-85 3.374354260241592 1105 86-87 2.903706637134654 1105 88-89 2.7289202520942197 1105 90-91 2.3781679456112332 1105 92-93 2.0710523915147707 1105 94-95 1.9524644990545106 1105 96-97 2.037133415518701 1105 98-99 1.4938724340709708 1105 100-101 1.54156167288639 1106 1 3.3857197756454838 1106 2 2.3866572867109923 1106 3 2.5324169494180566 1106 4 1.301679559528484 1106 5 1.6182774064108756 1106 6 1.8871731094676463 1106 7 2.4779122430796043 1106 8 2.5607457444044357 1106 9 2.7619849884708714 1106 10-11 2.515277571724596 1106 12-13 2.8254967123760615 1106 14-15 2.4805352060122488 1106 16-17 0.9457366630490043 1106 18-19 2.323888799257844 1106 20-21 1.7872846685383834 1106 22-23 2.6734726612393516 1106 24-25 2.8515907096854107 1106 26-27 2.73744861754691 1106 28-29 2.038030673245501 1106 30-31 1.417093035598132 1106 32-33 1.7312049767212052 1106 34-35 2.3144936031206598 1106 36-37 2.565095674180913 1106 38-39 1.091167277804292 1106 40-41 1.1828071273294611 1106 42-43 2.220996286231628 1106 44-45 2.5066211070782174 1106 46-47 2.481720027226803 1106 48-49 2.455522889661868 1106 50-51 2.460554443540662 1106 52-53 2.0779165154366908 1106 54-55 2.1060315113240797 1106 56-57 2.348543405511794 1106 58-59 2.5717106855972034 1106 60-61 2.7083527044997595 1106 62-63 2.398401860423153 1106 64-65 2.3893570878788317 1106 66-67 2.401842965948507 1106 68-69 2.5068637017386486 1106 70-71 2.1703985724974366 1106 72-73 2.470722960848626 1106 74-75 2.2622113391635352 1106 76-77 2.1514805480112322 1106 78-79 2.080325592898248 1106 80-81 2.1625789283234056 1106 82-83 2.3823263706868865 1106 84-85 2.492409336947002 1106 86-87 1.976780446457802 1106 88-89 1.8648265430359103 1106 90-91 1.9755127534710137 1106 92-93 2.0941872382699547 1106 94-95 1.598946035869787 1106 96-97 1.4830635723516998 1106 98-99 1.7045968258035895 1106 100-101 1.7097485291521757 1107 1 2.7871720444286687 1107 2 3.1800345336405194 1107 3 -0.15336296172331032 1107 4 1.7046293515642112 1107 5 0.5784341338551968 1107 6 1.4997606852463221 1107 7 2.3083420702874697 1107 8 1.6807274621145325 1107 9 -1.3123632053471894 1107 10-11 -0.7914158995123941 1107 12-13 2.0127805932755756 1107 14-15 1.6760377369103523 1107 16-17 -0.21854382956716734 1107 18-19 0.7616767968958484 1107 20-21 1.0150287525801858 1107 22-23 0.8054329533434199 1107 24-25 0.22244044937551166 1107 26-27 1.1089372059426879 1107 28-29 1.2627378084957641 1107 30-31 1.9555828643015865 1107 32-33 0.37317604738264265 1107 34-35 1.767251031429069 1107 36-37 2.7702424997926194 1107 38-39 0.7582281482888362 1107 40-41 1.4128203813449893 1107 42-43 0.942444390572188 1107 44-45 1.434270040697264 1107 46-47 1.663665234507743 1107 48-49 1.1197363386197274 1107 50-51 2.1991986015883462 1107 52-53 2.3207257607571705 1107 54-55 1.9649474798341906 1107 56-57 2.194359429977574 1107 58-59 2.124866314556968 1107 60-61 2.6498825763174416 1107 62-63 2.5876522478581414 1107 64-65 2.691675315760225 1107 66-67 2.0956984146639748 1107 68-69 1.6403437575396005 1107 70-71 1.0833626886686254 1107 72-73 1.7763343861194443 1107 74-75 1.3563609419298643 1107 76-77 1.491463129553079 1107 78-79 2.0981086009409076 1107 80-81 2.856139771732625 1107 82-83 2.694557712565711 1107 84-85 2.801989875720153 1107 86-87 2.730695409313036 1107 88-89 2.9154770599167286 1107 90-91 2.665086110836919 1107 92-93 2.4854366226286704 1107 94-95 2.4789062996092266 1107 96-97 2.700850383832668 1107 98-99 2.276016694449593 1107 100-101 1.7527486555036855 1108 1 2.752751270289796 1108 2 2.869827026882078 1108 3 2.605721108355496 1108 4 2.706409592746187 1108 5 2.152862895680471 1108 6 1.8784988231153292 1108 7 1.9236067569566906 1108 8 2.212202857027421 1108 9 2.3477870221351935 1108 10-11 -1.0651310263056928 1108 12-13 1.5070013803639846 1108 14-15 1.2521736583566465 1108 16-17 1.3945546939929798 1108 18-19 -0.18230875090494436 1108 20-21 -0.43301987358262295 1108 22-23 0.648789358653751 1108 24-25 -0.800323495259434 1108 26-27 0.2775341233111561 1108 28-29 -0.21872896407505493 1108 30-31 -0.011930138126128753 1108 32-33 1.3078033089845 1108 34-35 -1.0501893061308145 1108 36-37 0.5731155667372541 1108 38-39 1.1220880983754107 1108 40-41 1.7709475958284706 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2312 64-65 -2.004034065054608 2312 66-67 1.0691379948668676 2312 68-69 -0.4245164009223892 2312 70-71 -1.0244361014561782 2312 72-73 -1.2374421528900754 2312 74-75 -1.3381857960524854 2312 76-77 -1.680199268034432 2312 78-79 0.056586940230193505 2312 80-81 0.40362776251452814 2312 82-83 -1.0968452184361084 2312 84-85 -1.7435192865801135 2312 86-87 -0.46962042468855714 2312 88-89 -2.572995128784356 2312 90-91 -1.8377724853237627 2312 92-93 -1.3526510894303758 2312 94-95 -1.0631551595594342 2312 96-97 -0.8072920087720927 2312 98-99 -0.12667035998960152 2312 100-101 -0.40457145276742956 2313 1 -1.7128515550596966 2313 2 -0.5716020908774588 2313 3 -2.5456289373969554 2313 4 -2.106208949461422 2313 5 -2.1603006503746798 2313 6 -3.0337222688432313 2313 7 0.3877921078106574 2313 8 -3.4323896055074385 2313 9 -3.6567835783135045 2313 10-11 -5.244054086316503 2313 12-13 -3.815933140210678 2313 14-15 -3.4567908033668964 2313 16-17 -1.9462139936780076 2313 18-19 -4.312993164259396 2313 20-21 -6.410343765343619 2313 22-23 -5.297532794359178 2313 24-25 -1.8799943098389065 2313 26-27 -5.682029145906807 2313 28-29 -6.264065310412612 2313 30-31 -5.7235809114277565 2313 32-33 -5.278587542382734 2313 34-35 -0.3054330327804209 2313 36-37 -0.2510324389667069 2313 38-39 -3.159253880700774 2313 40-41 -6.6645139620464455 2313 42-43 -3.223855304050435 2313 44-45 -2.0138700836248447 2313 46-47 -3.650518293993361 2313 48-49 -3.1904836558107945 2313 50-51 -2.8505337802136133 2313 52-53 -4.65888900119479 2313 54-55 -4.063222049450413 2313 56-57 -3.3747788111053616 2313 58-59 -1.9106839547131926 2313 60-61 -0.1920787299614375 2313 62-63 -0.32181421490894735 2313 64-65 0.29547766532528996 2313 66-67 -0.26214416730884693 2313 68-69 -2.960801289635775 2313 70-71 -1.3070542670917753 2313 72-73 -2.8084809950830874 2313 74-75 -3.4114873511191455 2313 76-77 -2.1374396358349657 2313 78-79 -1.5829679133202639 2313 80-81 -1.8017526051606403 2313 82-83 -1.783392105482232 2313 84-85 -2.2256604648204643 2313 86-87 -1.7116852598740557 2313 88-89 -2.2250779528505937 2313 90-91 -3.857731054486935 2313 92-93 -4.293554168850971 2313 94-95 -3.581988640188481 2313 96-97 -2.9779983387270264 2313 98-99 -2.109988336961326 2313 100-101 -1.6882018846886044 2314 1 -2.2602191358891623 2314 2 -0.5309331892171798 2314 3 -2.3481155778486595 2314 4 -1.578994121203035 2314 5 -2.733473585106143 2314 6 -2.538414915210108 2314 7 -1.3144096969886974 2314 8 0.6032673649839211 2314 9 -1.993406385331049 2314 10-11 -2.5927960621852932 2314 12-13 -1.7001587905434086 2314 14-15 -4.137563766665949 2314 16-17 -2.055888323179925 2314 18-19 -1.5130755061293968 2314 20-21 -1.6617227816103437 2314 22-23 -2.495906332370872 2314 24-25 -2.954946417118599 2314 26-27 -2.3314428801946363 2314 28-29 -2.0393455248375645 2314 30-31 -2.1738218454198233 2314 32-33 -9.318546455470052 2314 34-35 -9.287493217965604 2314 36-37 -5.331139315353077 2314 38-39 -3.570947476519823 2314 40-41 -2.7093430186438745 2314 42-43 -1.6645523531866822 2314 44-45 -1.6709172453509957 2314 46-47 -3.0296479908408394 2314 48-49 -2.177626140854823 2314 50-51 -1.8115894365959484 2314 52-53 -1.107894807136848 2314 54-55 0.08288750724784677 2314 56-57 -0.8745582525250022 2314 58-59 -3.447140187488621 2314 60-61 -4.2400901317897635 2314 62-63 -3.4448575452174772 2314 64-65 -3.994891906833079 2314 66-67 -3.427727996373843 2314 68-69 -4.771468552860888 2314 70-71 -6.638772271393716 2314 72-73 -6.432051565426573 2314 74-75 -6.759295628997748 2314 76-77 -7.688435930450815 2314 78-79 -6.7728541471039545 2314 80-81 -5.993646972304557 2314 82-83 -5.022439796548982 2314 84-85 -4.846012980448615 2314 86-87 -4.934931504076516 2314 88-89 -5.377718144085385 2314 90-91 -5.702490372561817 2314 92-93 -5.820800505477978 2314 94-95 -5.400496235805877 2314 96-97 -5.575336091634198 2314 98-99 -5.58597790979187 2314 100-101 -5.198300316832181 2315 1 -25.0296156876133 2315 2 -13.058397582020778 2315 3 -8.135480377591644 2315 4 -5.4637392486393175 2315 5 -1.578045495579481 2315 6 -1.728859871303488 2315 7 -7.080000562270342 2315 8 -1.0923995337095853 2315 9 -4.513359801480117 2315 10-11 -3.0020626342564007 2315 12-13 -3.1271078160306622 2315 14-15 -3.25651656705147 2315 16-17 -5.3963520199029915 2315 18-19 -6.431157536542763 2315 20-21 -5.1397875438720675 2315 22-23 -7.333076363426773 2315 24-25 -24.358841790860303 2315 26-27 -10.359098025836097 2315 28-29 -6.517179375769242 2315 30-31 -4.692373060883522 2315 32-33 -6.0485595739108255 2315 34-35 -6.646518839083615 2315 36-37 -5.4024072528129174 2315 38-39 -3.8282476210903944 2315 40-41 -4.004872709155766 2315 42-43 -3.6188252167548995 2315 44-45 -8.364129790863956 2315 46-47 -4.8684223536594295 2315 48-49 -4.647551446059364 2315 50-51 -6.250146943556754 2315 52-53 -9.514221777580197 2315 54-55 -5.449008375168088 2315 56-57 -12.31258165153978 2315 58-59 -7.691419610277222 2315 60-61 -4.662950353464641 2315 62-63 -4.9267668137974745 2315 64-65 -4.758582606126286 2315 66-67 -7.85601229172617 2315 68-69 -5.2960355104535175 2315 70-71 -4.93345662564516 2315 72-73 -5.445885496675231 2315 74-75 -4.892623161659987 2315 76-77 -5.718555468895879 2315 78-79 -5.051942281608131 2315 80-81 -4.375846051336467 2315 82-83 -4.24562195546952 2315 84-85 -4.581559269803936 2315 86-87 -4.124654276618816 2315 88-89 -5.15801839574527 2315 90-91 -5.255449384127461 2315 92-93 -5.078405185280934 2315 94-95 -5.481222836791099 2315 96-97 -5.624769856894425 2315 98-99 -5.599064893085938 2315 100-101 -4.973587450051285 2316 1 -2.845113842594852 2316 2 -1.1549695912191318 2316 3 -4.785502915084102 2316 4 -0.8093192261782249 2316 5 -5.0536492329833145 2316 6 -0.9660536097005021 2316 7 -4.235284649272703 2316 8 -4.324335542510667 2316 9 -3.1398547248144446 2316 10-11 -2.2370660339798363 2316 12-13 -3.9503443527438584 2316 14-15 -3.7957509034942127 2316 16-17 -2.0268823003610734 2316 18-19 -6.269347508504595 2316 20-21 -3.1244108621549387 2316 22-23 -6.043011959677145 2316 24-25 -2.759965609551905 2316 26-27 -3.2552238690821085 2316 28-29 -5.945134270225019 2316 30-31 -9.47817367665365 2316 32-33 -6.415162066022706 2316 34-35 -3.536128673070152 2316 36-37 -5.31728046986807 2316 38-39 -5.94555183139958 2316 40-41 -3.632308859293662 2316 42-43 -3.728216093272362 2316 44-45 -1.6113431636187983 2316 46-47 -3.6317893786340285 2316 48-49 -3.5796135120216412 2316 50-51 -1.4644555773263228 2316 52-53 0.07512983022159503 2316 54-55 0.30897715499777334 2316 56-57 -1.831312981710223 2316 58-59 -4.309265484388138 2316 60-61 -6.894649527599238 2316 62-63 -3.080521407244028 2316 64-65 -4.261581238597085 2316 66-67 -3.9409473149894474 2316 68-69 -0.8597172963396993 2316 70-71 0.05306060598678286 2316 72-73 0.38759986923432876 2316 74-75 0.797781978416257 2316 76-77 0.003137822571197546 2316 78-79 -0.1572038311180357 2316 80-81 0.6550387171385452 2316 82-83 0.03126985692686546 2316 84-85 -0.3557450551400514 2316 86-87 -1.261635627798789 2316 88-89 -2.6225535504135884 2316 90-91 -2.360523757852567 2316 92-93 -2.3712535423359338 2316 94-95 -3.9152590342959304 2316 96-97 -5.160155248015418 2316 98-99 -3.0930882556798114 2316 100-101 -2.349748894895548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14533.0 3 4874.0 4 4721.0 5 5356.0 6 6485.0 7 8733.0 8 11326.0 9 13567.0 10 15998.0 11 18846.0 12 21340.0 13 23546.0 14 24891.0 15 27084.0 16 29220.0 17 29610.0 18 26729.0 19 25570.0 20 26946.0 21 28633.0 22 30819.0 23 33134.0 24 35602.0 25 39011.0 26 41693.0 27 44615.0 28 48271.0 29 51945.0 30 55021.0 31 58272.0 32 61992.0 33 65714.0 34 68330.0 35 63091.0 36 51760.0 37 28970.0 38 4438.0 39 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.361797209976444 18.06178359966578 13.609589590545973 16.9668295998118 2 14.990029275743561 23.30991935855158 41.082994882386984 20.61705648331788 3 21.48837397345033 32.20184357070537 29.737421250984365 16.572361204859938 4 11.422615461385124 17.582894622603533 38.3902802223678 32.60420969364354 5 11.086657327641031 41.6679020261943 32.17928823993284 15.066152406231826 6 24.20406550895432 36.935884964299326 20.986915263321844 17.873134263424515 7 25.28087600749003 38.150819973876935 20.911280702561687 15.657023316071347 8 24.86187360854266 31.449676046371533 20.827030946800402 22.861419398285403 9 28.838003470620695 19.04820751318018 21.819830576620827 30.293958439578304 10-11 23.412726126707824 27.512073634345125 26.925423483136445 22.149776755810606 12-13 29.98115260866822 25.538302876089137 27.005613035279936 17.474931479962706 14-15 25.978031553341786 25.66745855490821 30.149420606387217 18.20508928536278 16-17 18.52166421553697 29.66383263860486 31.588332042501722 20.226171103356453 18-19 17.85193479222976 29.096101868319852 35.27922967762094 17.772733661829452 20-21 20.891770309605715 26.79889555855768 33.542098995835715 18.76723513600089 22-23 19.761538925700854 26.74361761575655 32.54646484168329 20.948378616859305 24-25 19.777917101563954 27.009952447313356 34.458448785804954 18.753681665317732 26-27 21.37859257968995 25.112436857690295 35.50744643790281 18.001524124716948 28-29 18.805722227133934 25.800716780940196 37.02035495856233 18.373206033363545 30-31 18.192450342544785 28.862674679552768 35.38852947400413 17.556345503898314 32-33 18.639175275691304 28.267694828313815 33.81331340420889 19.279816491785986 34-35 19.746538019953576 26.412247554607955 34.08242044679098 19.758793978647493 36-37 21.004346736679267 26.288409791596308 35.09369995533429 17.61354351639014 38-39 21.93314478499433 24.92624436893022 35.48065229998619 17.659958546089264 40-41 19.207034563339256 26.575481755103336 36.82345934863896 17.39402433291845 42-43 18.39437797168086 28.70687252904192 34.315316021387396 18.583433477889823 44-45 18.33273049918585 29.629450689690145 32.54755969690283 19.490259114221185 46-47 18.988193339240656 27.99447875867865 31.029071425616735 21.988256476463967 48-49 20.439288650857453 28.046036702244614 31.227113552623585 20.287561094274352 50-51 21.807545966738793 28.945282420618746 31.22754798763767 18.01962362500479 52-53 19.34995249324428 29.30005841958681 32.75513480286983 18.59485428429907 54-55 17.76221319937647 29.076568341664967 33.30964073685573 19.85157772210284 56-57 19.456009739521786 30.896203206274258 30.377528387344544 19.270258666859412 58-59 19.11946339579697 29.141348815805912 31.011775113486806 20.727412674910305 60-61 19.29979823219187 27.920442104392528 32.191128746144756 20.588630917270844 62-63 22.480086704447523 27.21435846543574 31.1594543539387 19.14610047617804 64-65 21.151474924648813 28.02698497328705 32.34427603016224 18.477264071901896 66-67 18.709163485354356 28.853900499288287 32.947933851919885 19.489002163437473 68-69 18.256762316045634 31.0270536174881 31.649053917866183 19.067130148600086 70-71 18.73948324960567 30.678022108631037 29.78343711674404 20.799057525019254 72-73 18.80662378779621 29.665756547174816 29.141773439062295 22.38584622596668 74-75 19.254472550630986 30.02853008026834 30.18187218471779 20.535125184382885 76-77 20.785215275957185 28.352339584942833 30.90448737992442 19.957957759175564 78-79 19.06861052335463 28.47904783014435 32.10594683758949 20.346394808911526 80-81 18.406356630672178 31.366244444589615 30.660857030248 19.566541894490207 82-83 18.689003860021728 30.732123552056283 29.30040481879851 21.278467769123484 84-85 19.31472855930495 28.92062498176636 29.50127123865541 22.263375220273286 86-87 20.34390952728223 28.903551641940506 30.4954012604356 20.25713757034167 88-89 21.262972833296836 27.406164787038072 30.77804563616027 20.55281674350482 90-91 18.977283712780014 28.793632158661286 32.03790779885509 20.191176329703616 92-93 18.288723345799138 30.475254965530723 30.581799926241455 20.654221762428683 94-95 18.039733449802807 30.975334570313436 29.423593689920935 21.561338289962826 96-97 18.482574247334753 29.533206645989594 28.304124539785647 23.680094566890002 98-99 19.46266888249503 29.303429814798278 28.570333310826555 22.663567991880136 100-101 20.604954644963307 29.472296882960773 29.229493914235764 20.69325455784015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6921.0 1 5061.5 2 5006.5 3 6118.0 4 4746.5 5 3982.5 6 4589.0 7 6849.5 8 9204.0 9 9882.5 10 8647.0 11 6924.0 12 6151.5 13 5891.5 14 5795.5 15 5769.0 16 5635.5 17 5578.5 18 5709.5 19 5817.0 20 6047.5 21 6440.0 22 7064.0 23 7737.5 24 8413.5 25 9298.5 26 10504.5 27 11836.0 28 12891.5 29 14008.0 30 15530.5 31 17012.0 32 18404.5 33 20129.5 34 22183.0 35 23779.0 36 25052.5 37 26537.5 38 27283.5 39 27139.0 40 27084.5 41 27947.5 42 29265.5 43 30847.5 44 33289.5 45 35880.0 46 40057.0 47 50578.0 48 73146.5 49 72795.5 50 49525.5 51 36789.0 52 29444.5 53 25437.5 54 22560.5 55 20195.0 56 17835.0 57 15439.0 58 13714.0 59 12316.5 60 10597.0 61 8852.0 62 7311.5 63 5626.0 64 4086.5 65 2996.5 66 2177.0 67 1569.0 68 1154.0 69 894.0 70 698.0 71 541.0 72 443.5 73 374.5 74 296.0 75 234.5 76 169.5 77 120.5 78 87.0 79 65.5 80 47.5 81 36.5 82 27.0 83 16.0 84 9.0 85 7.5 86 6.5 87 3.5 88 1.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5854354394804604 2 3.734039215754434 3 1.1226971846102105 4 0.9070913548232353 5 0.9291646642814746 6 0.12453170257345242 7 1.7967152482247932 8 0.8924916855752661 9 0.142259872374558 10-11 1.0761607388823085 12-13 0.8202754644737036 14-15 0.6658926524557426 16-17 0.13739331595856824 18-19 0.15099360308539678 20-21 0.16468079300536798 22-23 0.6267429441449678 24-25 1.378669361811958 26-27 0.2182129135812554 28-29 0.2312917839492279 30-31 0.2080452867835625 32-33 0.22964063087951708 34-35 0.7308090389333204 36-37 0.2866488631811115 38-39 0.27561220845199186 40-41 0.2612732475834506 42-43 0.206046522541281 44-45 0.1712419538876399 46-47 0.21017440521555802 48-49 0.5982822793907419 50-51 0.2352458610372196 52-53 0.25892687216859833 54-55 0.2934272810462401 56-57 0.20856670354241855 58-59 0.19374977731159257 60-61 0.18366905330704236 62-63 0.17215443321563798 64-65 0.1671575226099342 66-67 0.18041019856419205 68-69 0.18757967899846267 70-71 0.19514022200187536 72-73 0.26587909562001233 74-75 0.22807638060294896 76-77 0.22420920630809993 78-79 0.21834326777096943 80-81 0.2275549638440929 82-83 0.24245879286806157 84-85 0.21043511359498607 86-87 0.1997460700384371 88-89 0.20000677841786513 90-91 0.2176045940292567 92-93 0.20591616835156698 94-95 0.21590998956297455 96-97 0.23815710460749917 98-99 0.2527567738554685 100-101 0.30281278270564893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150711.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.46956547198455 #Duplication Level Percentage of deduplicated Percentage of total 1 88.4911176555255 57.04983903381989 2 7.080023234244548 9.128920428866014 3 1.9184546847924595 3.7104581971878883 4 0.8098419692921232 2.0884063944495774 5 0.42744096387604924 1.377846660300757 6 0.25656311200917226 0.9924307408422863 7 0.1598665888203332 0.7214570660314713 8 0.11548209821564707 0.5956064553404647 9 0.09878883469427867 0.5731985921601472 >10 0.5726007526331652 6.875095537608196 >50 0.038285609544542264 1.727103199182807 >100 0.024224228303805204 2.8671058545333152 >500 0.003296787551219812 1.4970382027241507 >1k 0.0030101103728528717 4.515510786290919 >5k 5.733543567338804E-4 2.755936439496441 >10k+ 4.3001576755041023E-4 3.52404641116577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 14472 1.2576572223607838 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11937 1.0373586417441043 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11726 1.019022152391 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9557 0.8305299940645392 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6936 0.6027577732375896 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6832 0.5937198827507515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6498 0.5646943498410982 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4478 0.38915070769289595 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4076 0.35421578484954086 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3945 0.34283151894785047 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3416 0.29685994137537575 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3104 0.26974626991486134 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2963 0.25749297608174426 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2537 0.22047238620296494 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2456 0.21343325995840834 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2453 0.2131725515789803 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2246 0.1951836733984467 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2035 0.17684718404534241 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 0.17597815611391565 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1909 0.16589743210936542 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1762 0.15312272151739229 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1757 0.15268820755167892 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1453 0.1262697584363059 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1452 0.12618285564316323 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1353 0.11757947912203845 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1232 0.10706424115177486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 4.34513965713372E-5 0.0 8.69027931426744E-5 0.0 18-19 0.0 1.303541897140116E-4 0.0 1.303541897140116E-4 0.0 20-21 0.0 1.738055862853488E-4 0.0 2.607083794280232E-4 0.0 22-23 0.0 1.738055862853488E-4 0.0 0.0011297363108547672 0.0 24-25 0.0 1.738055862853488E-4 0.0 0.0026070837942802324 0.0 26-27 0.0 1.738055862853488E-4 0.0 0.003476111725706976 0.0 28-29 4.34513965713372E-5 1.738055862853488E-4 0.0 0.005822487140559184 0.0 30-31 8.69027931426744E-5 1.738055862853488E-4 0.0 0.012340196626259764 0.0 32-33 8.69027931426744E-5 1.738055862853488E-4 0.0 0.025419066994232263 0.0 34-35 8.69027931426744E-5 1.738055862853488E-4 0.0 0.046188834555331446 0.0 36-37 8.69027931426744E-5 1.738055862853488E-4 0.0 0.07842977081126365 0.0 38-39 8.69027931426744E-5 1.738055862853488E-4 0.0 0.11649319420775503 0.0 40-41 8.69027931426744E-5 1.738055862853488E-4 0.0 0.15894520865795148 0.0 42-43 8.69027931426744E-5 1.738055862853488E-4 0.0 0.20083235495272056 0.0 44-45 8.69027931426744E-5 1.738055862853488E-4 0.0 0.24936756492290418 0.0 46-47 8.69027931426744E-5 1.738055862853488E-4 0.0 0.3143274027970533 0.0 48-49 8.69027931426744E-5 1.738055862853488E-4 0.0 0.3888899993134679 0.0 50-51 8.69027931426744E-5 1.738055862853488E-4 0.0 0.4702310136950112 0.0 52-53 8.69027931426744E-5 1.738055862853488E-4 0.0 0.5464882146777079 0.0 54-55 8.69027931426744E-5 1.738055862853488E-4 0.0 0.6280030346455365 0.0 56-57 8.69027931426744E-5 1.738055862853488E-4 0.0 0.7265942534659007 0.0 58-59 8.69027931426744E-5 1.738055862853488E-4 0.0 0.8576871169216249 0.0 60-61 8.69027931426744E-5 1.738055862853488E-4 0.0 1.025452959083558 0.0 62-63 8.69027931426744E-5 1.738055862853488E-4 0.0 1.17727213870381 0.0 64-65 8.69027931426744E-5 1.738055862853488E-4 0.0 1.318098114991514 0.0 66-67 8.69027931426744E-5 2.607083794280232E-4 0.0 1.472741635388903 0.0 68-69 8.69027931426744E-5 2.607083794280232E-4 0.0 1.6503274931759582 0.0 70-71 8.69027931426744E-5 2.607083794280232E-4 0.0 1.8564174671138105 0.0 72-73 8.69027931426744E-5 2.607083794280232E-4 0.0 2.07606427678192 0.0 74-75 8.69027931426744E-5 2.607083794280232E-4 0.0 2.2958414406397436 0.0 76-77 8.69027931426744E-5 2.607083794280232E-4 0.0 2.5115341732198617 8.69027931426744E-5 78-79 8.69027931426744E-5 2.607083794280232E-4 0.0 2.759554744849054 8.69027931426744E-5 80-81 8.69027931426744E-5 2.607083794280232E-4 0.0 3.0344717309559046 8.69027931426744E-5 82-83 8.69027931426744E-5 2.607083794280232E-4 0.0 3.316340940514169 8.69027931426744E-5 84-85 8.69027931426744E-5 2.607083794280232E-4 0.0 3.6656032661545774 8.69027931426744E-5 86-87 8.69027931426744E-5 2.607083794280232E-4 0.0 4.03350624092409 8.69027931426744E-5 88-89 8.69027931426744E-5 2.607083794280232E-4 0.0 4.364388625814822 8.69027931426744E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 165 0.0 47.4198 2 ATAACGC 135 0.0 46.142967 3 TACCTGG 1105 0.0 36.73157 2 ACCTGGG 1475 0.0 26.963856 3 TCACGCA 180 6.148184E-9 26.602077 4 GTACCTG 1580 0.0 26.072767 1 GGAACGC 305 0.0 25.772236 8 TATCACG 200 1.5081241E-8 24.450836 2 TACATGG 13840 0.0 24.415503 2 GTACATG 13845 0.0 24.275236 1 CATGGGG 3205 0.0 23.456312 4 ATGGGAG 2560 0.0 23.42714 5 ACATGGG 14170 0.0 23.0289 3 CATGGGT 1295 0.0 22.925034 4 ATAGCCC 275 4.5474735E-11 22.652002 3 GAGTACT 10280 0.0 22.605581 12-13 TAACGCA 345 0.0 22.206953 4 ATCACGC 220 5.140282E-8 21.780771 3 ATGGGGT 830 0.0 21.560616 5 ATGGGAT 3345 0.0 21.399475 5 >>END_MODULE