##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1142795 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.065667070646967 31.0 27.0 31.0 10.0 33.0 2 26.974480112356108 31.0 26.0 31.0 16.0 34.0 3 27.283702676333025 31.0 26.0 31.0 16.0 34.0 4 31.140343631184944 35.0 30.0 37.0 19.0 37.0 5 31.655811409745404 35.0 32.0 37.0 19.0 37.0 6 31.60077179196619 35.0 32.0 37.0 17.0 37.0 7 31.671219247546585 35.0 32.0 37.0 17.0 37.0 8 31.648892408524713 35.0 32.0 37.0 18.0 37.0 9 32.64001680091355 35.0 31.0 39.0 17.0 39.0 10-11 31.634631758101847 35.0 29.5 39.0 14.0 39.0 12-13 31.349667700681223 35.0 29.5 38.0 15.5 39.0 14-15 31.976901806535732 36.0 29.0 39.0 16.5 40.0 16-17 32.02651700436211 36.0 29.0 39.0 17.0 40.0 18-19 32.30208130067072 36.0 30.0 39.0 17.5 40.0 20-21 32.33563850034345 36.0 30.0 39.0 17.5 40.0 22-23 32.447600838295585 36.0 30.0 39.0 17.0 40.0 24-25 32.37550348050175 36.0 30.0 39.0 16.0 41.0 26-27 32.30907336836441 36.0 30.0 39.0 16.0 41.0 28-29 31.829334220048217 36.0 30.0 39.0 14.0 40.5 30-31 31.48185457584256 35.5 29.5 39.0 12.5 40.0 32-33 31.270234381494493 35.0 29.0 39.0 10.0 40.0 34-35 30.72648681522058 35.0 27.5 38.0 9.0 40.0 36-37 30.195306244777058 35.0 26.0 38.0 9.0 40.0 38-39 29.673872391811305 35.0 25.0 38.0 8.0 40.0 40-41 29.45207276895681 34.5 25.0 38.0 7.5 40.0 42-43 29.187660954064377 34.0 24.0 38.0 2.0 40.0 44-45 28.77375644800686 34.0 24.0 38.0 2.0 40.0 46-47 27.967818375124146 33.0 22.5 38.0 2.0 40.0 48-49 27.498458166162784 33.0 21.5 38.0 2.0 40.0 50-51 26.765393180754202 32.5 19.5 37.5 2.0 39.5 52-53 26.737445473597624 33.0 19.0 37.5 2.0 39.0 54-55 26.90346518841962 33.0 19.5 38.0 2.0 40.0 56-57 26.55950542310738 33.0 18.0 38.0 2.0 40.0 58-59 26.216089062342764 33.0 15.0 37.5 2.0 40.0 60-61 25.580396309049306 31.5 11.0 37.0 2.0 39.0 62-63 24.909957603944715 31.0 8.0 36.0 2.0 39.0 64-65 24.267424603712826 31.0 7.5 36.0 2.0 39.0 66-67 23.92919946272079 30.5 6.5 35.0 2.0 38.5 68-69 23.35823747916293 30.0 2.0 35.0 2.0 38.0 70-71 22.710441067733058 29.0 2.0 34.5 2.0 37.5 72-73 22.169210138301267 29.0 2.0 34.0 2.0 37.0 74-75 21.450295109796595 28.0 2.0 34.0 2.0 36.0 76-77 20.659623117006987 26.5 2.0 33.5 2.0 36.0 78-79 20.169973617315442 26.0 2.0 33.0 2.0 35.0 80-81 19.711207609413762 26.0 2.0 33.0 2.0 35.0 82-83 19.041712643124967 25.0 2.0 33.0 2.0 35.0 84-85 18.463482076837927 24.5 2.0 32.0 2.0 35.0 86-87 17.65489567245219 21.0 2.0 32.0 2.0 34.0 88-89 16.782905507986996 19.0 2.0 31.0 2.0 34.0 90-91 15.974619244921442 11.5 2.0 31.0 2.0 34.0 92-93 15.23934170170503 4.5 2.0 31.0 2.0 34.0 94-95 14.271965663132933 2.0 2.0 30.5 2.0 34.0 96-97 13.176514160457476 2.0 2.0 29.0 2.0 34.0 98-99 12.210250744884252 2.0 2.0 29.0 2.0 34.0 100-101 10.987893279197056 2.0 2.0 26.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.9148999646368736 1101 2 3.7678790960288957 1101 3 3.290656849059438 1101 4 3.1324159265961136 1101 5 2.473729192122665 1101 6 2.6471019973488623 1101 7 2.4965910800637197 1101 8 2.245292926352196 1101 9 2.566610583174068 1101 10-11 3.2170068560365905 1101 12-13 3.399252787057339 1101 14-15 2.9591904774298463 1101 16-17 2.8984810464248163 1101 18-19 2.9093979665615564 1101 20-21 2.7449553760147296 1101 22-23 2.931426753044633 1101 24-25 3.0374443681527623 1101 26-27 2.7998821787864046 1101 28-29 2.8864504936168522 1101 30-31 3.1236702327919694 1101 32-33 2.9164825603304187 1101 34-35 3.3499470964549793 1101 36-37 3.2487580633590163 1101 38-39 3.1488236012087008 1101 40-41 3.223918257552132 1101 42-43 2.838574193871114 1101 44-45 3.0899447724387095 1101 46-47 3.222979230139746 1101 48-49 3.1102392727585872 1101 50-51 3.17376255728119 1101 52-53 2.9943117288418932 1101 54-55 2.670314432385169 1101 56-57 3.036185244393682 1101 58-59 2.9738935272478813 1101 60-61 3.013885146133255 1101 62-63 2.9549359307657603 1101 64-65 3.140677264652421 1101 66-67 2.8645540385014385 1101 68-69 2.850678242312817 1101 70-71 2.5898851942953804 1101 72-73 2.4705281621337853 1101 74-75 2.4075953343506526 1101 76-77 2.4990524269344405 1101 78-79 2.1885545516729508 1101 80-81 2.216947824457449 1101 82-83 2.225927453951183 1101 84-85 1.929659939654659 1101 86-87 1.977327373730251 1101 88-89 2.4802482324529542 1101 90-91 1.697181092857658 1101 92-93 1.315112894227676 1101 94-95 1.4476373162868956 1101 96-97 1.5764756084738583 1101 98-99 1.216899256234914 1101 100-101 1.0088389387038443 1102 1 3.5086052506578227 1102 2 2.3765651724716435 1102 3 2.645402661748122 1102 4 2.741498293413212 1102 5 1.832935626081639 1102 6 2.063113432535829 1102 7 2.162463958515591 1102 8 2.1511518658153967 1102 9 2.2300767401934465 1102 10-11 1.9248166018818864 1102 12-13 2.9204800411127927 1102 14-15 3.101070762961772 1102 16-17 2.549978437211319 1102 18-19 2.7662588976193447 1102 20-21 2.6484220836386427 1102 22-23 2.5880132512069878 1102 24-25 2.716726278402767 1102 26-27 2.48376598384052 1102 28-29 2.575352044800251 1102 30-31 2.795381330588757 1102 32-33 2.2674016714518395 1102 34-35 2.5580111346611396 1102 36-37 2.827950572818917 1102 38-39 2.79298092631387 1102 40-41 2.7407982714041843 1102 42-43 2.5753558813436257 1102 44-45 2.537385584261635 1102 46-47 2.776500527306087 1102 48-49 2.5566806648897256 1102 50-51 3.0027342815645177 1102 52-53 2.9664696132453656 1102 54-55 3.1878468401715843 1102 56-57 3.2496922982898973 1102 58-59 2.9589192571430267 1102 60-61 3.1345617231986935 1102 62-63 3.3023489225697205 1102 64-65 3.1095970872128618 1102 66-67 2.632166682410265 1102 68-69 2.8097712227569396 1102 70-71 2.5710976254969786 1102 72-73 2.536867315695215 1102 74-75 2.764169371462849 1102 76-77 2.5404031902824364 1102 78-79 2.5315996556031806 1102 80-81 2.4140562812360784 1102 82-83 2.322244851388408 1102 84-85 2.2577380182766404 1102 86-87 2.1102791719249936 1102 88-89 1.596243221951621 1102 90-91 1.3072940319919475 1102 92-93 1.1462787051631178 1102 94-95 1.2160233422334112 1102 96-97 1.118116525925922 1102 98-99 0.8704701078100427 1102 100-101 0.9272860745097304 1103 1 3.5643100466636994 1103 2 3.635132385702139 1103 3 3.104709393903697 1103 4 2.9065306214096474 1103 5 2.4706737945748323 1103 6 2.3093253361325736 1103 7 2.116805378981663 1103 8 2.134669121372344 1103 9 2.5325665600412606 1103 10-11 3.2131881443851853 1103 12-13 2.3563016484382153 1103 14-15 3.0311514773963033 1103 16-17 2.8308547712905856 1103 18-19 2.5145611673277912 1103 20-21 2.762505890707544 1103 22-23 2.904144786456307 1103 24-25 2.9316020712018016 1103 26-27 2.7441927230839624 1103 28-29 2.164765378705784 1103 30-31 2.7345513306510476 1103 32-33 2.8830141452616935 1103 34-35 2.605381114927251 1103 36-37 2.9865491415410865 1103 38-39 3.272869458579226 1103 40-41 3.146673653743921 1103 42-43 3.029095110247713 1103 44-45 3.1185949233529335 1103 46-47 2.957517926489185 1103 48-49 2.7738700499829214 1103 50-51 2.779208387673439 1103 52-53 2.785745695926959 1103 54-55 3.015379868994412 1103 56-57 3.1861199041354453 1103 58-59 3.109073992687488 1103 60-61 3.136057511664717 1103 62-63 3.1307786630805303 1103 64-65 3.005980338535089 1103 66-67 2.798404431791983 1103 68-69 3.0511711312515075 1103 70-71 2.945070698535897 1103 72-73 2.6114975389283224 1103 74-75 2.635373401577965 1103 76-77 2.459619884416881 1103 78-79 2.1147553885706785 1103 80-81 2.0474396356922604 1103 82-83 2.2344823529717566 1103 84-85 1.8667744910122437 1103 86-87 1.7057891762649255 1103 88-89 1.4448006962909936 1103 90-91 1.652215861297634 1103 92-93 1.5127769889171656 1103 94-95 1.7019947014489833 1103 96-97 1.6351184010258546 1103 98-99 1.3092320711760301 1103 100-101 1.111490589838965 1104 1 3.6194883051230278 1104 2 3.454488914155707 1104 3 2.91899395469844 1104 4 2.646843841705394 1104 5 1.677691497947169 1104 6 2.2585805541525446 1104 7 2.286766845597935 1104 8 2.2667797995096883 1104 9 2.324368903256392 1104 10-11 3.2690707108618824 1104 12-13 3.249737741984969 1104 14-15 3.1403232912337167 1104 16-17 3.195709733371263 1104 18-19 2.8664441056357077 1104 20-21 2.5071243464273962 1104 22-23 2.1995830010693993 1104 24-25 2.593981370245693 1104 26-27 2.495956376854828 1104 28-29 2.415976035333909 1104 30-31 2.1430503199894027 1104 32-33 2.2490578815682234 1104 34-35 2.9599449019602524 1104 36-37 2.9911254473437516 1104 38-39 3.2316497979362673 1104 40-41 3.2075362478636897 1104 42-43 3.0345212674875306 1104 44-45 3.165125392138453 1104 46-47 3.0900716808648134 1104 48-49 2.8574380961117036 1104 50-51 2.995131041354057 1104 52-53 3.192592139816071 1104 54-55 2.8233083552682103 1104 56-57 3.1243993162893062 1104 58-59 3.039130031993036 1104 60-61 2.9104438375042037 1104 62-63 3.122100679084202 1104 64-65 2.858592011728028 1104 66-67 2.837796827029557 1104 68-69 3.08198189291517 1104 70-71 3.0055061617337664 1104 72-73 2.684363563665954 1104 74-75 2.984550725031081 1104 76-77 3.1339900613827503 1104 78-79 2.9584159718164216 1104 80-81 3.015839071974437 1104 82-83 3.0425621501201903 1104 84-85 2.7603873400874477 1104 86-87 2.42283750142996 1104 88-89 2.445164724471457 1104 90-91 2.506910942744277 1104 92-93 2.3627447929485488 1104 94-95 2.313817169688969 1104 96-97 1.930046704776668 1104 98-99 1.5429880345547264 1104 100-101 1.4935108546397817 1105 1 3.4402081834747342 1105 2 3.2573406870015305 1105 3 2.9239335555635755 1105 4 2.359011234942681 1105 5 -0.17306131557446847 1105 6 1.7571503501325907 1105 7 1.9592933009991818 1105 8 2.1511511776192016 1105 9 2.9016640096649198 1105 10-11 2.4741437524605843 1105 12-13 3.1598979922976618 1105 14-15 2.9716324136416468 1105 16-17 2.7719740134861617 1105 18-19 2.832731768237089 1105 20-21 2.785276091073918 1105 22-23 2.5201598590191168 1105 24-25 2.10932637998053 1105 26-27 2.314769428950683 1105 28-29 2.4684139260258426 1105 30-31 2.264997812612382 1105 32-33 2.372727690635987 1105 34-35 2.4763483975947587 1105 36-37 2.6339883245094633 1105 38-39 3.090719720071103 1105 40-41 2.989583949215504 1105 42-43 3.108354200768513 1105 44-45 2.572534860898614 1105 46-47 2.669781943118359 1105 48-49 2.560462302989368 1105 50-51 2.5201012566807144 1105 52-53 2.634120744571092 1105 54-55 2.523623270002414 1105 56-57 2.8157316053038564 1105 58-59 2.7989545891357537 1105 60-61 2.71357490490864 1105 62-63 2.5393134543141436 1105 64-65 2.8750825339278947 1105 66-67 2.562715298654176 1105 68-69 2.828124727120791 1105 70-71 3.1110326184842627 1105 72-73 2.485573300386985 1105 74-75 2.2274152212956864 1105 76-77 2.301431534330984 1105 78-79 2.439624642630683 1105 80-81 2.593867495255463 1105 82-83 2.547226166861673 1105 84-85 2.3470273455923873 1105 86-87 2.2677807857450745 1105 88-89 2.2940893054958913 1105 90-91 2.115084963493029 1105 92-93 1.9437260478311433 1105 94-95 2.059016541478597 1105 96-97 2.0495948952312713 1105 98-99 2.1209270700845018 1105 100-101 1.9653398831939395 1106 1 3.1972353302786907 1106 2 2.8226160008270966 1106 3 2.4473228043354176 1106 4 1.6138986160499762 1106 5 1.8996489600155009 1106 6 2.3607265943554907 1106 7 2.1483537572939433 1106 8 0.6764013557646287 1106 9 1.969398336571551 1106 10-11 1.3712511286916111 1106 12-13 1.9631506786229629 1106 14-15 2.0419385678421698 1106 16-17 1.8833879830512466 1106 18-19 2.1021920515725085 1106 20-21 1.8340953472140384 1106 22-23 2.6822944251592276 1106 24-25 2.7907659836288374 1106 26-27 2.504265314431123 1106 28-29 1.8575172180456114 1106 30-31 2.0171800099234254 1106 32-33 2.2193283121581153 1106 34-35 2.434890689790972 1106 36-37 1.9509400238619428 1106 38-39 2.7494466234657686 1106 40-41 2.0271163959301504 1106 42-43 2.3904335115174504 1106 44-45 2.4981438219167593 1106 46-47 2.625489399363282 1106 48-49 2.2623330657212115 1106 50-51 2.146753227191418 1106 52-53 2.2357124689245467 1106 54-55 2.1957178157214656 1106 56-57 2.273361954549717 1106 58-59 2.3918229123865267 1106 60-61 2.373058505426439 1106 62-63 2.483323255343553 1106 64-65 2.1429816032611555 1106 66-67 1.6346806636805553 1106 68-69 1.928662733050441 1106 70-71 2.24304713683156 1106 72-73 2.440103447712243 1106 74-75 2.6200043041127685 1106 76-77 2.5982127259454195 1106 78-79 1.7707410924920026 1106 80-81 1.9199221229281456 1106 82-83 2.1823173140104686 1106 84-85 2.2751987046567876 1106 86-87 1.860312350151549 1106 88-89 1.9562231981183125 1106 90-91 1.552449839006755 1106 92-93 1.3588648396212353 1106 94-95 1.3583665295573333 1106 96-97 1.5491766407466532 1106 98-99 1.1642837610112764 1106 100-101 1.1160502423001013 1107 1 3.1290886800419493 1107 2 3.2976196809742753 1107 3 -0.4074534047052367 1107 4 0.8805451484528923 1107 5 1.5525927667597408 1107 6 2.0372323274373585 1107 7 2.1030122707356433 1107 8 1.8919439476801934 1107 9 2.3510216512196394 1107 10-11 1.7805543334259681 1107 12-13 2.225512344827738 1107 14-15 2.2792994219956917 1107 16-17 1.2924268391854774 1107 18-19 0.13801007745171745 1107 20-21 -1.3438990313179495 1107 22-23 0.5269811565181541 1107 24-25 1.374346445468305 1107 26-27 1.5052833157069117 1107 28-29 2.588612492045524 1107 30-31 3.1220213558413725 1107 32-33 1.7980091677214922 1107 34-35 1.1571784904313525 1107 36-37 1.3789017475668608 1107 38-39 2.4288570811655 1107 40-41 2.5335488785332494 1107 42-43 1.629419850396463 1107 44-45 1.2580133546520997 1107 46-47 2.155688376109591 1107 48-49 1.5040223258423033 1107 50-51 1.8515660303716182 1107 52-53 1.5847949892275572 1107 54-55 2.0650884863652266 1107 56-57 2.5100085491523068 1107 58-59 2.3373698651901087 1107 60-61 2.148515275618699 1107 62-63 1.703436140834647 1107 64-65 1.4693218506637962 1107 66-67 1.9489838926036782 1107 68-69 2.1034147809745924 1107 70-71 0.7026951005180067 1107 72-73 1.4790528814533523 1107 74-75 1.9746360716960965 1107 76-77 2.022944213422896 1107 78-79 1.8094144288063134 1107 80-81 1.9360930006277002 1107 82-83 1.8252721992071557 1107 84-85 1.8443588639708786 1107 86-87 1.9007417904996444 1107 88-89 1.6164333086056608 1107 90-91 1.5606778187683616 1107 92-93 2.048882068929993 1107 94-95 1.9434843599222447 1107 96-97 1.992986149417634 1107 98-99 1.4280829307147833 1107 100-101 1.5393131264435382 1108 1 2.46797896961451 1108 2 2.4731226118194485 1108 3 1.8883880679667442 1108 4 1.7447498915094322 1108 5 1.9279179132678372 1108 6 0.0762027634462683 1108 7 0.40209171409711786 1108 8 -0.45128231192730084 1108 9 1.5111710392575262 1108 10-11 2.7126744697227423 1108 12-13 1.772611187316425 1108 14-15 2.050341277840065 1108 16-17 -0.024291652776984307 1108 18-19 0.47918860585928513 1108 20-21 1.1986257249118424 1108 22-23 -0.4355331321634317 1108 24-25 1.4679015241081501 1108 26-27 1.4963852164688163 1108 28-29 2.072431520982274 1108 30-31 0.9451693274806026 1108 32-33 1.1112454234275724 1108 34-35 1.4491349448841966 1108 36-37 0.27913173299291927 1108 38-39 1.5119049824972457 1108 40-41 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40-41 -0.15905863969007328 2312 42-43 -3.078674039969961 2312 44-45 -2.9879966728805307 2312 46-47 -0.8784396192455866 2312 48-49 -0.007042177143951278 2312 50-51 -0.910908359095874 2312 52-53 -1.3299366826664887 2312 54-55 -1.0337411886393113 2312 56-57 -0.6208567752115179 2312 58-59 -0.6971412946857036 2312 60-61 -0.20560821880826552 2312 62-63 -0.5389555754495916 2312 64-65 -1.874720568493089 2312 66-67 0.8227554445622118 2312 68-69 0.16597977462895486 2312 70-71 -2.269128503253068 2312 72-73 -2.691928847957204 2312 74-75 -0.6791839865482601 2312 76-77 -0.30114395122741655 2312 78-79 -0.26556994761852337 2312 80-81 -1.7320359099719873 2312 82-83 0.1910604598159189 2312 84-85 1.3986389706211284 2312 86-87 1.8095373404369397 2312 88-89 1.9139538660514752 2312 90-91 2.1590946298188953 2312 92-93 2.32462624888681 2312 94-95 1.9383501226664404 2312 96-97 1.4915798185023643 2312 98-99 0.76171046970261 2312 100-101 1.5889141082817098 2313 1 -7.987696020043106 2313 2 -4.575990280285513 2313 3 -2.1212484643774623 2313 4 0.36740123508464606 2313 5 0.6000537736491403 2313 6 -3.105886328316984 2313 7 -1.0130902067117624 2313 8 -2.286460913823756 2313 9 0.3007403051935711 2313 10-11 -1.581647619041803 2313 12-13 -2.271138856507246 2313 14-15 -4.049469170189742 2313 16-17 -3.971553666889374 2313 18-19 -1.0943182925703034 2313 20-21 -3.324607830695129 2313 22-23 -1.943951950864335 2313 24-25 -2.9002348130746505 2313 26-27 -2.5389296478180547 2313 28-29 -3.165201440025175 2313 30-31 -5.309053521127911 2313 32-33 -6.355984990898907 2313 34-35 -5.301711728373551 2313 36-37 -2.3429084164983784 2313 38-39 -1.715613476031546 2313 40-41 -3.435596935023316 2313 42-43 -3.0302416927215496 2313 44-45 -3.32243752734119 2313 46-47 -1.9866137166063247 2313 48-49 -2.9296831142155746 2313 50-51 -3.460503078862505 2313 52-53 -1.1024164520426218 2313 54-55 -2.6479766031775043 2313 56-57 -3.092718965623117 2313 58-59 -1.8850492817159754 2313 60-61 -2.1900371013566513 2313 62-63 -4.31504456634617 2313 64-65 -5.613108529042059 2313 66-67 -4.513618086732176 2313 68-69 -3.3360268323403197 2313 70-71 -2.0939131280743233 2313 72-73 -1.9854047758281013 2313 74-75 -3.136848252154909 2313 76-77 -3.1682132748000633 2313 78-79 -3.5456573664265427 2313 80-81 -3.987808422652197 2313 82-83 -1.8741678602910774 2313 84-85 -1.7750062238723672 2313 86-87 -3.115877169139157 2313 88-89 -3.9196427539581222 2313 90-91 -3.4696005652946553 2313 92-93 -1.8017985756125885 2313 94-95 -1.0112538502913484 2313 96-97 -0.9376828738710028 2313 98-99 -1.0075740869121326 2313 100-101 -0.5132568620727849 2314 1 -7.392615049879005 2314 2 -3.360414678764446 2314 3 -25.06457219156286 2314 4 -13.248853049314175 2314 5 -6.39919592367194 2314 6 -5.281238316903291 2314 7 -4.618416787320985 2314 8 -3.5974812686259767 2314 9 -3.5351224576255227 2314 10-11 -14.35827625900452 2314 12-13 -13.102592170059875 2314 14-15 -4.843410191851891 2314 16-17 -3.1067047789857334 2314 18-19 -3.1769382699475415 2314 20-21 -3.977226216521231 2314 22-23 -4.213543661156557 2314 24-25 -5.424376654385632 2314 26-27 -4.372685026408284 2314 28-29 -3.41930821037003 2314 30-31 -4.028552580407506 2314 32-33 -4.087091005256976 2314 34-35 -3.3214455414458683 2314 36-37 -4.288379316691067 2314 38-39 -1.629764923490459 2314 40-41 -2.660237272886885 2314 42-43 -4.629175069254124 2314 44-45 -5.970595306899991 2314 46-47 -5.257740136923612 2314 48-49 -3.7239694456333616 2314 50-51 -3.3776358426478943 2314 52-53 -1.5551530673817666 2314 54-55 -3.4219223426519534 2314 56-57 -3.3062454450551577 2314 58-59 -1.0843029579148968 2314 60-61 -3.831044269020879 2314 62-63 -4.76451423877084 2314 64-65 -4.143723151986542 2314 66-67 -5.117323274620475 2314 68-69 -4.377880364162817 2314 70-71 -4.851336577306686 2314 72-73 -4.35785758349483 2314 74-75 -3.8692147181724508 2314 76-77 -3.392216992208791 2314 78-79 -4.122716862018516 2314 80-81 -6.306488117301743 2314 82-83 -5.715028377886242 2314 84-85 -4.281119485472217 2314 86-87 -3.753303346744895 2314 88-89 -2.938955942801348 2314 90-91 -4.734666302040729 2314 92-93 -4.4512978054155194 2314 94-95 -4.9988312252551115 2314 96-97 -4.503156729801521 2314 98-99 -5.307838682288677 2314 100-101 -4.284215601109555 2315 1 -24.74369986250228 2315 2 -24.672051759632296 2315 3 -13.055444402516207 2315 4 -28.93327119527078 2315 5 -15.275543687833714 2315 6 -12.024990092251258 2315 7 -8.994874573046989 2315 8 -8.046569289878143 2315 9 -5.209704675740273 2315 10-11 -29.45196462192365 2315 12-13 -29.175077377160466 2315 14-15 -23.485409023622918 2315 16-17 -13.582246985258163 2315 18-19 -8.67844856596556 2315 20-21 -6.4307241021068045 2315 22-23 -3.1664044218656535 2315 24-25 -4.924721722018891 2315 26-27 -4.809928726173439 2315 28-29 -17.285667016696067 2315 30-31 -16.47754058205583 2315 32-33 -9.250425018723615 2315 34-35 -6.9799686119676885 2315 36-37 -4.7955007124846425 2315 38-39 -4.690454818709924 2315 40-41 -8.815665736865828 2315 42-43 -8.052686077411725 2315 44-45 -4.310953017011169 2315 46-47 -3.6798268659224966 2315 48-49 -4.683621537443354 2315 50-51 -3.1362785366159116 2315 52-53 -6.7375348142041425 2315 54-55 -6.328521035995848 2315 56-57 -7.111270229912993 2315 58-59 -7.3540928367311444 2315 60-61 -4.720743650099571 2315 62-63 -3.4510758048377888 2315 64-65 -4.203517871751615 2315 66-67 -6.4239793792169735 2315 68-69 -6.57497099392625 2315 70-71 -6.846636656122133 2315 72-73 -6.267227820061198 2315 74-75 -6.762780857135688 2315 76-77 -6.656575806536399 2315 78-79 -6.306197824287121 2315 80-81 -7.839557598920139 2315 82-83 -7.049484931370724 2315 84-85 -6.231862831167973 2315 86-87 -6.306931857933087 2315 88-89 -5.946034330165272 2315 90-91 -5.729018195011754 2315 92-93 -5.403709478103368 2315 94-95 -5.260528517924476 2315 96-97 -5.312184299205124 2315 98-99 -5.178382308839481 2315 100-101 -4.825324658484801 2316 1 -1.2184581868846642 2316 2 1.3913209578757595 2316 3 1.8913891403383225 2316 4 1.977577354675514 2316 5 -1.4726403123274316 2316 6 -2.545706545801824 2316 7 -2.46215440694062 2316 8 -3.6644777962934114 2316 9 -3.665529837020756 2316 10-11 -3.044338413545564 2316 12-13 -3.962402833658853 2316 14-15 -2.3095909650020126 2316 16-17 -2.5594024572473195 2316 18-19 -4.26338907574479 2316 20-21 -1.943041007090212 2316 22-23 -2.387213253565303 2316 24-25 -2.3935626159082055 2316 26-27 -2.6693380071271946 2316 28-29 -1.7871757871734069 2316 30-31 0.6224647558325032 2316 32-33 -1.5706313226607982 2316 34-35 -7.520318145610549 2316 36-37 -3.523372441938676 2316 38-39 -1.6349336991913361 2316 40-41 -1.3716825174437552 2316 42-43 -1.7238618072317813 2316 44-45 -0.9044488545476383 2316 46-47 0.2067092367218244 2316 48-49 0.40945053712149715 2316 50-51 0.6677147485384403 2316 52-53 0.6946260719288944 2316 54-55 -1.3343714705435303 2316 56-57 -0.4918415146882431 2316 58-59 -3.7899406785902094 2316 60-61 -3.6621674065349943 2316 62-63 -4.724554343258603 2316 64-65 -5.327702754416116 2316 66-67 -3.130946194981391 2316 68-69 -2.710568063970239 2316 70-71 -3.265632566646051 2316 72-73 -2.8320804398750035 2316 74-75 -3.1688988397189206 2316 76-77 -2.9529081325702613 2316 78-79 -2.951442310466348 2316 80-81 -3.045433960354007 2316 82-83 -3.9818212052632216 2316 84-85 -3.169658457585509 2316 86-87 -2.323997304801491 2316 88-89 -3.4717847392218157 2316 90-91 -4.077088820721201 2316 92-93 -4.168037350813249 2316 94-95 -5.153648742420808 2316 96-97 -4.842104797349936 2316 98-99 -4.633603961929029 2316 100-101 -4.469108306245175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14622.0 3 5544.0 4 4397.0 5 4780.0 6 6179.0 7 8269.0 8 10553.0 9 12885.0 10 15632.0 11 18847.0 12 21734.0 13 24026.0 14 25597.0 15 27893.0 16 29247.0 17 29206.0 18 25618.0 19 24925.0 20 26212.0 21 28215.0 22 30533.0 23 33206.0 24 36047.0 25 38849.0 26 42485.0 27 45368.0 28 49353.0 29 52252.0 30 55099.0 31 58661.0 32 62547.0 33 65476.0 34 65869.0 35 59709.0 36 48479.0 37 28917.0 38 5518.0 39 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.36230405121419 17.80328127609668 13.54393602631188 17.290478646377252 2 14.966001686877425 23.216864458140083 40.996516544167235 20.82061731081526 3 21.395961641340342 32.24452982345997 29.62689173714857 16.73261679805112 4 11.497037520034068 17.58978001841279 38.41584630798836 32.49733615356478 5 11.249428021928894 41.51421223060052 32.160683323135615 15.075676424334972 6 24.38187613261033 36.93056359653541 20.888290571864754 17.79926969898951 7 25.26084366897905 38.10936514610048 21.031637586714318 15.598153598206151 8 24.772861853054845 31.549427293017846 20.842224394619464 22.835486459307848 9 28.809182611620514 19.045562683727564 21.835243457634483 30.31001124701744 10-11 23.443138328783462 27.532360146589024 26.930670813808128 22.093830710819393 12-13 29.93961724285377 25.523803434344245 27.087254615957406 17.44932470684458 14-15 25.986847025184208 25.684592543714945 30.112834048168924 18.215726382931926 16-17 18.706242333444422 29.713719154772782 31.389161258565412 20.19087725321738 18-19 17.887179415308356 29.224596623554884 35.143164093551015 17.74505986758575 20-21 20.868978569050416 26.925193193964986 33.341510855660886 18.864317381323705 22-23 19.795295117665873 26.749214611512024 32.50478681686537 20.950703453956738 24-25 19.810004079667003 27.048367497805696 34.42520296452881 18.716425457998493 26-27 21.37893984534427 25.169481981735252 35.45627472228442 17.995303450636058 28-29 18.790382029790447 25.843266782706664 36.94534604052011 18.42100514698278 30-31 18.210262938259657 28.869072667466213 35.390935678666864 17.529728715607266 32-33 18.549615276231098 28.293116694211108 33.90455730142473 19.252710728133067 34-35 19.835157973608624 26.457890135988098 33.96179538468617 19.74515650571711 36-37 21.076747753325158 26.254998635192408 35.03975905778766 17.62849455369477 38-39 21.819176116631528 24.99024084881756 35.50062687160298 17.689956162947933 40-41 19.144662541103038 26.68742590939239 36.707367399168874 17.460544150335704 42-43 18.35228126496336 28.826206630074836 34.346961153637054 18.47455095132475 44-45 18.31448520356462 29.63435957175834 32.518248287107845 19.532906937569194 46-47 18.917700359729565 28.02762162233215 30.978061316145766 22.076616701792513 48-49 20.3180695141094 28.098638272287698 31.266712650649396 20.316579562953503 50-51 21.79264050461705 28.973712456462447 31.23111290902947 18.002534129891032 52-53 19.42724607491417 29.329605510024443 32.71791810010766 18.525230314953728 54-55 17.786179401702096 29.137357784250682 33.19187920948755 19.884583604559673 56-57 19.485677400016566 30.923160994113374 30.398654135295512 19.192507470574547 58-59 19.082909200527187 29.160094360786292 31.04091304225905 20.71608339642747 60-61 19.320919160985465 27.926149268821337 32.25785114119767 20.49508042899553 62-63 22.460919360971754 27.224148777158437 31.28777298621467 19.02715887565514 64-65 21.0408042578356 28.115163063714217 32.396740915960315 18.44729176248987 66-67 18.730991838892706 28.88259280625835 32.904011168246015 19.482404186602924 68-69 18.35939826965733 31.055410912582303 31.566401637132635 19.01878918062773 70-71 18.727008358366668 30.657143119998352 29.840232048831545 20.77561647280344 72-73 18.909600442711106 29.71588760641125 29.09914161190833 22.27537033896931 74-75 19.340875837883623 30.053998770424815 30.06355142152651 20.541573970165057 76-77 20.916363298483613 28.407183361193827 30.75254886251176 19.923904477810805 78-79 19.12822731255213 28.581277631607204 32.03335757091245 20.257137484928215 80-81 18.35675361998695 31.517083200201263 30.59219335466076 19.533969825151026 82-83 18.710794567808385 30.761820740481678 29.34232825867729 21.18505643303264 84-85 19.24168393929966 29.053693422084727 29.66437776891627 22.040244869699347 86-87 20.271603813022715 28.98867433746671 30.67560473499106 20.06411711451951 88-89 21.37941006995642 27.450830034156613 30.896463180311418 20.273296715575547 90-91 18.91294840253925 28.857232505409886 32.14637233153982 20.08344676051104 92-93 18.237527309821544 30.620126845521717 30.731250323160747 20.411095521495994 94-95 18.07959418104015 30.9255004778983 29.466025376838157 21.528879964223393 96-97 18.524200181332567 29.564003907243308 28.296605012424962 23.615190898999163 98-99 19.44029449875794 29.33498795212422 28.646625608149144 22.578091940968697 100-101 20.51231440664956 29.56817334473759 29.3417988025528 20.57771344606005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6481.0 1 4814.5 2 4928.5 3 6170.5 4 4822.5 5 4047.5 6 4630.0 7 6705.0 8 9006.5 9 9852.5 10 8732.5 11 6914.5 12 6058.5 13 5860.0 14 5842.5 15 5693.0 16 5590.0 17 5624.5 18 5703.5 19 5950.0 20 6235.0 21 6467.5 22 7004.5 23 7626.5 24 8351.0 25 9258.0 26 10379.0 27 11634.0 28 12826.0 29 13974.0 30 15256.5 31 16817.5 32 18409.0 33 20328.5 34 22116.0 35 23404.5 36 24883.0 37 26370.5 38 27015.5 39 27039.5 40 27145.5 41 27782.5 42 28919.0 43 30603.5 44 32955.5 45 35676.5 46 40314.5 47 50780.5 48 70847.5 49 70466.5 50 49622.0 51 37184.0 52 29767.0 53 25465.0 54 22487.0 55 20037.5 56 17674.5 57 15301.5 58 13524.5 59 12052.0 60 10491.5 61 8942.5 62 7348.5 63 5662.5 64 4095.0 65 2915.5 66 2130.5 67 1602.5 68 1197.5 69 898.5 70 674.0 71 537.0 72 460.5 73 397.0 74 306.5 75 250.0 76 204.5 77 149.5 78 106.0 79 74.5 80 52.0 81 35.0 82 24.5 83 18.5 84 11.5 85 6.5 86 3.5 87 2.5 88 2.0 89 2.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.646240139307575 2 2.6847334823831046 3 1.0404315734668073 4 0.9601897103154985 5 0.176497097029651 6 0.09398011016849041 7 0.09756780524941043 8 0.10491820492739291 9 0.10141801460454414 10-11 1.8989845072825835 12-13 1.4638233453944058 14-15 0.5399043572994281 16-17 0.09332382448295626 18-19 0.10067422416093876 20-21 0.12731942299362528 22-23 0.14350780323680098 24-25 0.15492717416509524 26-27 0.1526520504552435 28-29 0.5856255933916407 30-31 0.1878727155789096 32-33 0.16726534505313728 34-35 0.14897685061625227 36-37 0.14075140335755756 38-39 0.47261319834265986 40-41 0.12740692775169649 42-43 0.1282382229533731 44-45 0.13331349892150385 46-47 0.14525789839822542 48-49 0.15908365017347817 50-51 0.17234062102126804 52-53 0.19102288686947352 54-55 0.18747894416758912 56-57 0.15912740255251379 58-59 0.14377031751101466 60-61 0.12115033754960425 62-63 0.1257443373483433 64-65 0.11988151855757156 66-67 0.11765014722675546 68-69 0.11261862363766029 70-71 0.12981330859865506 72-73 0.14390157464812148 74-75 0.1531770790036708 76-77 0.17430947807787048 78-79 0.17540328755376075 80-81 0.17592831610218806 82-83 0.16884043069841922 84-85 0.15575846936677182 86-87 0.15383336468920497 88-89 0.15116446956803278 90-91 0.1599586977541904 92-93 0.15068319339864106 94-95 0.20869884799986 96-97 0.20554867670929608 98-99 0.22335589497678937 100-101 0.25113865566440174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1142795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.10465600362747 #Duplication Level Percentage of deduplicated Percentage of total 1 88.73516328541446 57.77072281122623 2 6.961513850830299 9.064539290455892 3 1.8582913783149035 3.6295026281909566 4 0.7545024039800676 1.9648647786012905 5 0.4039532992183126 1.314962029356932 6 0.2567153491960255 1.0028018700155006 7 0.17108650462086758 0.7796969621143224 8 0.12127522370170664 0.6316465376690064 9 0.09861240295785377 0.5778113915035948 >10 0.5674346726069912 6.8584502412125605 >50 0.040368428057642264 1.8023402087371305 >100 0.02423919522046269 2.9863851953035465 >500 0.002994252572664958 1.3760892162062937 >1k 0.00285166911682377 4.176816937614826 >5k 5.70333823364754E-4 2.6927682126087538 >10k+ 4.2775036752356547E-4 3.370601689183117 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13783 1.206078080495627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11430 1.0001793847540459 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11097 0.9710403003163297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8833 0.7729295280430873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6951 0.6082455733530511 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6818 0.5966074405295788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6406 0.5605554802042361 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4022 0.3519441369624473 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3928 0.34371868970375263 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3800 0.33251808067063643 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3150 0.27563998792434335 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3075 0.2690771310690019 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2670 0.2336377040501577 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.2300500089692377 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2314 0.20248601017680334 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2302 0.20143595307994872 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2166 0.1895353059822628 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.17413446856172804 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.1730844114648734 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1666 0.14578292694665274 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1612 0.14105767001080682 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1510 0.13213218468754237 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1450 0.12688189920326917 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1340 0.11725637581543495 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1198 0.10483070016932171 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1184 0.10360563355632463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 8.750475807122012E-5 0.0 0.0 0.0 9 0.0 8.750475807122012E-5 0.0 0.0 0.0 10-11 0.0 1.7500951614244023E-4 0.0 0.0 0.0 12-13 0.0 1.7500951614244023E-4 0.0 0.0 0.0 14-15 0.0 1.7500951614244023E-4 0.0 0.0 0.0 16-17 0.0 1.7500951614244023E-4 0.0 8.750475807122012E-5 0.0 18-19 0.0 1.7500951614244023E-4 0.0 3.0626665324927046E-4 0.0 20-21 0.0 1.7500951614244023E-4 0.0 4.812761693917107E-4 0.0 22-23 0.0 1.7500951614244023E-4 0.0 8.750475807122012E-4 0.0 24-25 0.0 1.7500951614244023E-4 0.0 0.001531333266246352 0.0 26-27 0.0 1.7500951614244023E-4 0.0 0.002668895121172214 0.0 28-29 0.0 1.7500951614244023E-4 0.0 0.005425295000415648 0.0 30-31 0.0 1.7500951614244023E-4 0.0 0.012731942299362527 0.0 32-33 0.0 1.7500951614244023E-4 0.0 0.025945160768116766 0.0 34-35 0.0 1.7500951614244023E-4 0.0 0.049308931173132536 0.0 36-37 0.0 1.7500951614244023E-4 0.0 0.08172944403851959 0.0 38-39 0.0 1.7500951614244023E-4 0.0 0.12303168984813549 0.0 40-41 0.0 1.7500951614244023E-4 0.0 0.16774662122252898 0.0 42-43 0.0 1.7500951614244023E-4 0.0 0.21149900025813903 0.0 44-45 0.0 1.7500951614244023E-4 0.0 0.2675020454237199 0.0 46-47 0.0 1.7500951614244023E-4 0.0 0.3378996232920165 0.0 48-49 0.0 1.7500951614244023E-4 0.0 0.41389750567687117 0.0 50-51 0.0 1.7500951614244023E-4 0.0 0.49199550225543515 0.0 52-53 0.0 1.7500951614244023E-4 0.0 0.5671183370595776 0.0 54-55 0.0 1.7500951614244023E-4 0.0 0.6533980285178007 0.0 56-57 0.0 1.7500951614244023E-4 0.0 0.7459780625571515 0.0 58-59 0.0 1.7500951614244023E-4 0.0 0.8762726473251983 0.0 60-61 0.0 1.7500951614244023E-4 0.0 1.035181287982534 0.0 62-63 0.0 1.7500951614244023E-4 0.0 1.181270481582436 0.0 64-65 0.0 1.7500951614244023E-4 0.0 1.3158527994959726 0.0 66-67 0.0 1.7500951614244023E-4 0.0 1.46986117370132 0.0 68-69 0.0 1.7500951614244023E-4 0.0 1.6453082136341162 0.0 70-71 0.0 1.7500951614244023E-4 0.0 1.8436815001815723 0.0 72-73 0.0 1.7500951614244023E-4 0.0 2.052117833907219 0.0 74-75 0.0 1.7500951614244023E-4 0.0 2.2601603962215444 0.0 76-77 0.0 1.7500951614244023E-4 0.0 2.478353510472132 0.0 78-79 0.0 1.7500951614244023E-4 0.0 2.72743580432186 0.0 80-81 0.0 1.7500951614244023E-4 0.0 2.9923127070034434 0.0 82-83 0.0 1.7500951614244023E-4 0.0 3.272327932831348 0.0 84-85 0.0 1.7500951614244023E-4 0.0 3.6130277083816433 0.0 86-87 0.0 1.7500951614244023E-4 0.0 3.988948149055605 0.0 88-89 0.0 1.7500951614244023E-4 0.0 4.313940820532116 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1055 0.0 51.578945 2 ACCTGGG 1425 0.0 37.169994 3 GTACCTG 1490 0.0 36.30031 1 ATAACGC 160 1.8189894E-12 36.11403 3 TATAACG 150 6.8030204E-10 32.68215 2 ATCACGC 135 3.7500104E-7 28.53454 3 ATGGGCG 155 1.1507527E-6 25.095823 5 ATGGGAG 2645 0.0 25.000942 5 TATCACG 160 1.4128073E-6 24.511616 2 GTACATG 13155 0.0 23.86237 1 TCACGCA 160 1.7946822E-6 23.847088 4 TACATGG 13215 0.0 23.445086 2 GCTTATA 2290 0.0 22.956766 1 GAGTACT 10270 0.0 22.40256 12-13 ATGGGAT 3485 0.0 22.3234 5 ACATGGG 13465 0.0 22.314793 3 TGGGATA 960 0.0 21.75558 6 CATGGGG 2935 0.0 21.612694 4 ATACTCT 2330 0.0 21.07699 5 AGTACTT 10370 0.0 20.886898 12-13 >>END_MODULE