##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1608748 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97414138199395 31.0 31.0 31.0 30.0 34.0 2 31.192190448721615 31.0 31.0 34.0 30.0 34.0 3 31.149898554652438 31.0 31.0 34.0 28.0 34.0 4 35.087190784386365 35.0 35.0 37.0 32.0 37.0 5 34.74365282816203 35.0 35.0 37.0 32.0 37.0 6 34.74331032579372 35.0 35.0 37.0 32.0 37.0 7 34.63007382138159 35.0 35.0 37.0 30.0 37.0 8 34.66909857852193 35.0 35.0 37.0 31.0 37.0 9 35.946694572425265 37.0 35.0 39.0 30.0 39.0 10-11 35.81636682687406 37.0 35.0 39.0 30.0 39.0 12-13 35.778775482549165 37.0 35.0 39.0 30.0 39.0 14-15 36.47636578258373 38.0 35.0 40.0 30.0 41.0 16-17 36.54081994196729 38.0 35.0 40.0 30.5 41.0 18-19 36.641577487586616 38.0 35.0 40.0 30.5 41.0 20-21 36.60467891801575 38.0 35.0 40.0 30.0 41.0 22-23 36.45481455144 38.0 35.0 40.0 30.0 41.0 24-25 36.21901130568616 38.0 34.5 40.0 29.5 41.0 26-27 35.78228349001833 38.0 34.0 40.0 28.0 41.0 28-29 35.50314810026182 38.0 34.0 40.0 27.0 41.0 30-31 35.221394836232896 37.5 34.0 40.0 27.0 41.0 32-33 34.876312511344224 37.0 33.0 40.0 27.0 41.0 34-35 34.69635611046603 37.0 33.0 40.0 25.5 41.0 36-37 34.53442086641289 37.0 33.0 40.0 25.0 41.0 38-39 34.42803596337028 37.0 33.0 40.0 25.0 41.0 40-41 34.182855549781564 37.0 33.0 40.0 24.0 41.0 42-43 33.812296580943695 37.0 33.0 40.0 23.5 41.0 44-45 33.511310658972064 37.0 33.0 40.0 22.0 41.0 46-47 33.01013769714088 37.0 31.5 40.0 19.5 41.0 48-49 32.581845944796825 36.5 31.0 40.0 16.0 41.0 50-51 32.303953136227676 36.0 31.0 40.0 14.0 41.0 52-53 31.945858207749133 36.0 31.0 39.0 9.0 41.0 54-55 31.770792255841187 36.0 30.5 39.0 8.5 40.0 56-57 31.414905566316165 35.0 30.0 39.0 8.0 40.0 58-59 31.04481683893313 35.0 29.0 39.0 7.0 40.0 60-61 30.767023797387782 35.0 28.5 38.5 4.0 40.0 62-63 30.41138264041354 35.0 28.0 38.0 2.0 40.0 64-65 29.99388406388073 34.5 28.0 38.0 2.0 40.0 66-67 28.355060581271896 33.5 24.5 37.5 2.0 39.5 68-69 24.822603042863147 29.5 13.0 35.0 2.0 39.0 70-71 24.233573561552213 28.5 10.5 35.0 2.0 38.0 72-73 26.020537399269493 31.0 20.0 35.0 2.0 38.0 74-75 26.60406415423671 31.5 22.0 35.0 2.0 38.0 76-77 26.583811137605146 31.5 23.5 34.5 2.0 36.5 78-79 27.371571868310014 32.5 26.0 35.0 2.0 37.0 80-81 27.213578198698613 33.0 26.0 35.0 2.0 37.0 82-83 26.799393689999924 32.5 25.0 35.0 2.0 36.0 84-85 26.450358601844414 32.0 24.5 35.0 2.0 36.0 86-87 26.001944058360912 32.0 23.5 35.0 2.0 35.5 88-89 25.462719456372284 32.0 20.5 34.0 2.0 35.0 90-91 24.962808656172378 31.0 19.5 34.0 2.0 35.0 92-93 24.436335585187983 31.0 15.5 34.0 2.0 35.0 94-95 23.130161467178205 30.0 3.5 34.0 2.0 35.0 96-97 22.33094337957219 29.5 2.0 34.0 2.0 35.0 98-99 22.028235932538845 30.0 2.0 34.0 2.0 35.0 100-101 20.861331296138363 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.468562990873437 1101 2 0.3750754122495472 1101 3 0.3778061333923972 1101 4 0.43694168553585655 1101 5 0.5759209375453551 1101 6 0.5458203631309857 1101 7 0.5528704518467222 1101 8 0.5370566348510266 1101 9 0.7723809585499595 1101 10-11 0.8455910751949673 1101 12-13 0.8686136210716242 1101 14-15 0.8969308891944721 1101 16-17 0.8499430638172427 1101 18-19 0.9041043535259021 1101 20-21 1.0048804738926975 1101 22-23 0.9414019772508126 1101 24-25 0.8539045888332097 1101 26-27 1.0927382556318364 1101 28-29 1.09943041321349 1101 30-31 1.1425123379395998 1101 32-33 0.9878675736805889 1101 34-35 1.1863680252693456 1101 36-37 1.0717902794544258 1101 38-39 0.960972098711558 1101 40-41 1.367087107068528 1101 42-43 1.4486321078691518 1101 44-45 1.26997364473457 1101 46-47 1.263528516266554 1101 48-49 0.9994083761949213 1101 50-51 0.9242366536899667 1101 52-53 1.1920066531394582 1101 54-55 0.9918599751096053 1101 56-57 0.8405203326576505 1101 58-59 0.8915456930301637 1101 60-61 0.7629866210984737 1101 62-63 0.7109203477028352 1101 64-65 0.32898186689330444 1101 66-67 -2.3783554707617256 1101 68-69 -7.593270253178304 1101 70-71 -11.040234319188407 1101 72-73 -10.894059715581923 1101 74-75 -9.102051653703828 1101 76-77 -3.984920520705387 1101 78-79 -1.2555959034373494 1101 80-81 -0.6350112397863263 1101 82-83 -0.5653914133776468 1101 84-85 -0.5478414106175755 1101 86-87 -0.4729638546915744 1101 88-89 -0.5472737977690407 1101 90-91 -0.5295988654247736 1101 92-93 -1.0497543171417263 1101 94-95 -4.184820390223848 1101 96-97 -1.490706613878686 1101 98-99 -0.9022269054054384 1101 100-101 -0.8518742604073921 1102 1 0.455501900642151 1102 2 0.2492693732770377 1102 3 0.43842489355735026 1102 4 0.2059275353107779 1102 5 0.40329062816526573 1102 6 0.4312413064387144 1102 7 0.48919200246286465 1102 8 0.40655294044228185 1102 9 0.6931841650409112 1102 10-11 0.6199111273369979 1102 12-13 0.6280798962670033 1102 14-15 0.5759394597354444 1102 16-17 0.5537093505861961 1102 18-19 0.6478141124908561 1102 20-21 0.7102125442844311 1102 22-23 0.831598545379606 1102 24-25 0.828037027142976 1102 26-27 0.7839928926782633 1102 28-29 0.7207111821865411 1102 30-31 0.5766193752857234 1102 32-33 0.4806286036074354 1102 34-35 0.43461736892550107 1102 36-37 0.8623249179627734 1102 38-39 0.9600654000449538 1102 40-41 0.8953759836393402 1102 42-43 1.0032469208339947 1102 44-45 0.7596293002560799 1102 46-47 0.8072321838733245 1102 48-49 0.6391541838176522 1102 50-51 0.5539710095592909 1102 52-53 0.6166611208461319 1102 54-55 0.7806174598659794 1102 56-57 0.40905603106232746 1102 58-59 0.4790724188642237 1102 60-61 0.5536832897346784 1102 62-63 0.37185951330858913 1102 64-65 0.46822513746534256 1102 66-67 -2.7143416921094996 1102 68-69 -7.592900203168252 1102 70-71 -11.025566633234469 1102 72-73 -9.041508045636075 1102 74-75 -7.469295375036374 1102 76-77 -3.699789221479598 1102 78-79 -1.1138411658835174 1102 80-81 -0.44355954730245273 1102 82-83 -0.17331261556809707 1102 84-85 -0.2266629764621122 1102 86-87 -0.2261965384830802 1102 88-89 -0.10595367862207539 1102 90-91 0.02961949149226939 1102 92-93 -0.4273774082623554 1102 94-95 -3.700604622134037 1102 96-97 -0.5770583167178671 1102 98-99 0.2233869512161739 1102 100-101 0.45047060792790106 1103 1 0.40452887666079107 1103 2 0.2854646858146488 1103 3 0.39461474156147247 1103 4 0.22660971130245855 1103 5 0.4179882974334035 1103 6 0.4046330003478076 1103 7 0.5627940929478328 1103 8 0.3902032728881295 1103 9 0.6099102113666746 1103 10-11 0.7426386517348149 1103 12-13 0.6203576317835768 1103 14-15 0.7042281098753094 1103 16-17 0.5529868646327003 1103 18-19 0.5102682537406196 1103 20-21 0.8410420821341518 1103 22-23 0.8753491171094296 1103 24-25 0.7037513885147391 1103 26-27 0.7986048024725321 1103 28-29 0.9506958237179575 1103 30-31 0.8563965691635147 1103 32-33 0.8179859118793402 1103 34-35 0.9284715742090128 1103 36-37 0.6993361792348693 1103 38-39 0.8273497555910936 1103 40-41 0.9138873917557362 1103 42-43 0.8608275166848003 1103 44-45 0.7008112403339837 1103 46-47 0.824954003009303 1103 48-49 0.8329203427079861 1103 50-51 0.6864417129193754 1103 52-53 0.6205151816926247 1103 54-55 0.7064797488074106 1103 56-57 0.800280519875681 1103 58-59 0.6968154220947973 1103 60-61 0.6899957166134456 1103 62-63 0.6225961420525188 1103 64-65 0.49436402327825846 1103 66-67 -2.3305304327632257 1103 68-69 -7.109677464449984 1103 70-71 -11.231233498903237 1103 72-73 -8.941189622214168 1103 74-75 -7.063291068406233 1103 76-77 -3.4860095095832833 1103 78-79 -0.5953682743027535 1103 80-81 -0.17029315249833132 1103 82-83 0.0815901181430192 1103 84-85 0.002866688492172642 1103 86-87 0.040817539310690876 1103 88-89 0.21640023538013153 1103 90-91 0.4282731580257355 1103 92-93 0.4252480954617148 1103 94-95 1.1709496524792335 1103 96-97 1.1555647075042188 1103 98-99 0.9850544524077769 1103 100-101 1.056283354975367 1104 1 0.4056445995867968 1104 2 0.21202573711628858 1104 3 0.24465149196140246 1104 4 0.049087264734659186 1104 5 0.32327915402998286 1104 6 0.40957568893981033 1104 7 0.443395526453628 1104 8 0.26012186955129124 1104 9 0.6830927479609485 1104 10-11 0.5888436324948927 1104 12-13 0.5426200272370991 1104 14-15 0.7263825360166152 1104 16-17 0.6372170398336223 1104 18-19 0.5686692630555257 1104 20-21 0.7221218112861081 1104 22-23 0.6480208711844071 1104 24-25 0.6213151972973705 1104 26-27 0.9075742419929114 1104 28-29 0.9256166782488364 1104 30-31 0.8828535543816898 1104 32-33 0.6156107071099584 1104 34-35 0.6922253513334482 1104 36-37 0.8460255206580953 1104 38-39 0.7487666516475073 1104 40-41 0.7090189245596719 1104 42-43 0.9412847709255061 1104 44-45 0.7647320155992787 1104 46-47 0.6400724690043731 1104 48-49 0.5461618360712919 1104 50-51 0.5945756212883779 1104 52-53 0.803473324668488 1104 54-55 0.580755549749945 1104 56-57 0.8229997131923916 1104 58-59 0.8398663538255242 1104 60-61 0.6327047255056009 1104 62-63 0.6597032370414944 1104 64-65 0.5268226730733687 1104 66-67 -2.357980339325934 1104 68-69 -7.127185246887617 1104 70-71 -8.38555095029903 1104 72-73 -7.913830090409462 1104 74-75 -6.644879057991215 1104 76-77 -3.159862020380217 1104 78-79 -0.3434472852606163 1104 80-81 0.2880287713971157 1104 82-83 0.4626404229551575 1104 84-85 0.37198001903920286 1104 86-87 0.5648644308994157 1104 88-89 0.616702765633157 1104 90-91 0.40073347720175434 1104 92-93 0.49259080134802247 1104 94-95 1.4932515606176437 1104 96-97 1.726408909828379 1104 98-99 1.2730905158394492 1104 100-101 1.1629261699118842 1105 1 0.44963785026321546 1105 2 0.4104376391172977 1105 3 0.5012967510819095 1105 4 0.4580605945000684 1105 5 0.3562062643085824 1105 6 0.5600064414745205 1105 7 0.5800990616720156 1105 8 0.6041942835494467 1105 9 0.7203738409351104 1105 10-11 0.7722572441263296 1105 12-13 0.7249140167711872 1105 14-15 0.824783845742175 1105 16-17 0.8058144739013571 1105 18-19 0.76375481890841 1105 20-21 0.8090667388869761 1105 22-23 0.8730231011371288 1105 24-25 1.0587761731246772 1105 26-27 0.8613445345626971 1105 28-29 1.0611262596123723 1105 30-31 0.9450199906199117 1105 32-33 1.0177761770353229 1105 34-35 1.1967809589212308 1105 36-37 1.23690650064799 1105 38-39 1.0959554245788539 1105 40-41 1.1617457850835677 1105 42-43 1.272193030670401 1105 44-45 1.0824733336500074 1105 46-47 1.3641102444035411 1105 48-49 1.0689519529208198 1105 50-51 0.9386656890789382 1105 52-53 1.1126163166703975 1105 54-55 1.1916901229369898 1105 56-57 1.2964595591769914 1105 58-59 1.2904338767940366 1105 60-61 1.2783290007709631 1105 62-63 1.3319670850479177 1105 64-65 1.3386992154573356 1105 66-67 1.9807782831579388 1105 68-69 -5.010475096142812 1105 70-71 -4.810172973312145 1105 72-73 -1.1067535212660573 1105 74-75 1.1907585598246193 1105 76-77 1.219927732243697 1105 78-79 1.0943667664148222 1105 80-81 1.1078132836321224 1105 82-83 1.2789424923512875 1105 84-85 1.1251595599662672 1105 86-87 1.3698541359510017 1105 88-89 1.2881907759206719 1105 90-91 1.2451264469041305 1105 92-93 1.430155841635834 1105 94-95 2.1649133213883154 1105 96-97 2.220699301705409 1105 98-99 1.9310557211437605 1105 100-101 1.6891699260442543 1106 1 0.14320268375342238 1106 2 0.1874319842086578 1106 3 0.24873448732561698 1106 4 0.11605440921029953 1106 5 0.12223815440763275 1106 6 0.20210096136754885 1106 7 0.4097682831322871 1106 8 0.30277439351015545 1106 9 0.4206207296148321 1106 10-11 0.45178005051480596 1106 12-13 0.488891146886381 1106 14-15 0.3821821955845621 1106 16-17 0.41480872935083113 1106 18-19 0.3493656162729408 1106 20-21 0.5499368067159978 1106 22-23 0.6519027270316755 1106 24-25 0.6280786845913653 1106 26-27 0.5892921650289935 1106 28-29 0.7412209998750185 1106 30-31 0.5224224863992717 1106 32-33 0.724065955619885 1106 34-35 0.7597753899193975 1106 36-37 0.69809903646739 1106 38-39 0.43625467905722104 1106 40-41 0.4546609016927121 1106 42-43 0.6515658736626477 1106 44-45 0.7938493551939345 1106 46-47 0.645330830661436 1106 48-49 0.5978054332668421 1106 50-51 0.47518495087805235 1106 52-53 0.6959006271002295 1106 54-55 0.5383903679581294 1106 56-57 0.6103760876457578 1106 58-59 0.8082436641933768 1106 60-61 0.7952801870318496 1106 62-63 0.7537694844622571 1106 64-65 0.6646525008322648 1106 66-67 1.4364564426607238 1106 68-69 -4.987710727296353 1106 70-71 -5.678447030951872 1106 72-73 -1.8195750181360637 1106 74-75 0.4930533919701965 1106 76-77 0.6276101347954999 1106 78-79 0.48712893156504933 1106 80-81 0.6146027223245447 1106 82-83 0.7442781638436777 1106 84-85 0.6228858310134342 1106 86-87 0.71413731838717 1106 88-89 0.6168286488682462 1106 90-91 0.6831675663818153 1106 92-93 0.8877122786698841 1106 94-95 1.625037624796203 1106 96-97 1.5918862535826577 1106 98-99 1.3560147286587387 1106 100-101 1.279696553417896 1107 1 -0.02352906312108871 1107 2 -0.054868964776261464 1107 3 -0.07286224699013033 1107 4 -0.12992349348402144 1107 5 0.07971877314717801 1107 6 0.06996067153745145 1107 7 0.13389749590523792 1107 8 -0.10938402513755818 1107 9 0.04113538452273957 1107 10-11 -0.014522760044975769 1107 12-13 0.17985005458765357 1107 14-15 -0.08840794924309137 1107 16-17 -0.1956031987119644 1107 18-19 -0.04641662441520822 1107 20-21 0.07674345357456502 1107 22-23 -0.10657182410997024 1107 24-25 0.1456926350411436 1107 26-27 0.10480762992796855 1107 28-29 -0.03779845208408261 1107 30-31 0.11458511263068516 1107 32-33 0.06806635640159442 1107 34-35 0.19679319942260065 1107 36-37 0.17975703045504332 1107 38-39 0.11013151484190331 1107 40-41 0.06241109607496753 1107 42-43 0.11163612283088042 1107 44-45 0.01698394886714283 1107 46-47 -0.2070897316877378 1107 48-49 -0.2707718759334483 1107 50-51 -0.012266274081284223 1107 52-53 0.06903870108191512 1107 54-55 0.10606988242545157 1107 56-57 0.06953973986586703 1107 58-59 -0.09944326036570672 1107 60-61 -0.1205010298525444 1107 62-63 -0.056979456072536294 1107 64-65 -0.18773247809392046 1107 66-67 0.6122121768200941 1107 68-69 -5.774619710830905 1107 70-71 -5.889215136593439 1107 72-73 -2.253195859284485 1107 74-75 -0.05278227067273278 1107 76-77 -0.23525915636710693 1107 78-79 -0.31207256393755145 1107 80-81 -0.1798928654870693 1107 82-83 -0.12242227586130738 1107 84-85 -0.22176708859081629 1107 86-87 -0.24813332850385095 1107 88-89 -0.3402767832534117 1107 90-91 -0.23265672517252156 1107 92-93 -0.0792823797589115 1107 94-95 0.6671236062072943 1107 96-97 0.7885194687103549 1107 98-99 0.5010512001066338 1107 100-101 0.3299170926066388 1108 1 -0.04122886534883108 1108 2 -0.07496382771455856 1108 3 -0.0025538737364563247 1108 4 -0.1357003990911494 1108 5 -0.016961151834216537 1108 6 0.1542521090436182 1108 7 0.0687230846763427 1108 8 0.08707774001100432 1108 9 0.015520218212216719 1108 10-11 0.06665605720086631 1108 12-13 0.09754848273120587 1108 14-15 0.08212804265556173 1108 16-17 0.014462319539866542 1108 18-19 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-0.20485897001210418 2316 88-89 0.4187811877610841 2316 90-91 0.36759266312034455 2316 92-93 0.6505548120348053 2316 94-95 1.2988955178705126 2316 96-97 1.5685382120443165 2316 98-99 0.5234785010665455 2316 100-101 0.3379124185367459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 25.0 4 60.0 5 200.0 6 522.0 7 1086.0 8 2232.0 9 3499.0 10 5708.0 11 8578.0 12 10894.0 13 13689.0 14 16584.0 15 21503.0 16 25782.0 17 28891.0 18 24248.0 19 18180.0 20 17128.0 21 17283.0 22 18407.0 23 21120.0 24 24798.0 25 30706.0 26 37874.0 27 46796.0 28 56021.0 29 67699.0 30 78928.0 31 91941.0 32 106048.0 33 122896.0 34 144459.0 35 165955.0 36 164603.0 37 143378.0 38 66531.0 39 4490.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.310606559884405 21.57559584725519 15.90884503805688 19.204952554803523 2 15.083913501388299 23.92955418970656 39.19295815453712 21.793574154368013 3 19.893668865478 28.616228271923262 31.788198027285812 19.701904835312927 4 11.704008334431496 19.374258740337204 38.69008694960305 30.23164597562825 5 11.200898090811103 41.061671637412 33.829206102144155 13.908224169632746 6 26.574578492094474 39.36346774013084 18.115018635609804 15.946935132164889 7 24.841615964712933 37.25437420901223 20.9414401758386 16.96256965043624 8 25.381911896704768 34.22991046453515 20.011773130409484 20.376404508350593 9 26.265580438950042 18.273029710060246 22.07157367095406 33.38981618003565 10-11 23.791358248774824 28.64640701962023 26.543809223072852 21.0184255085321 12-13 26.824959533749226 27.02909343166239 27.46182124235741 18.684125792230976 14-15 23.10787643558842 27.612932541330277 29.113322907005944 20.16586811607536 16-17 18.63586578829918 31.32904468211808 29.72571114572753 20.30937838385521 18-19 18.082877849532146 29.330794683256162 34.19615733911678 18.390170128094912 20-21 20.035835331523437 27.370702984775424 33.70839737124688 18.885064312454254 22-23 19.123691218264142 27.74353099428873 33.604455141513775 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19.212490880269804 29.047301259883923 32.08542756561928 19.654780294227 50-51 19.127624729484705 29.68269909241263 32.060402534225865 19.129273643876804 52-53 19.09756825858948 30.33798032958214 31.29630666442715 19.268144747401227 54-55 18.402352509415948 29.546852694469237 31.7914402744387 20.259354521676116 56-57 19.543153994775242 30.17903592595794 30.466111523257382 19.811698556009436 58-59 19.724084764045223 29.40132362221124 30.892745567979407 19.98184604576413 60-61 19.375647304416155 29.457495179019688 31.763577384970976 19.40328013159318 62-63 19.957580971609588 29.281965945427153 31.442669663681254 19.31778341928201 64-65 19.496038616110976 29.934386179758864 31.31457646294575 19.254998741184405 66-67 19.18145028505969 29.553846081814395 31.250470157301756 20.014233475824163 68-69 19.315017283125833 29.736659122109334 31.28040528154527 19.667918313219563 70-71 19.298072284773177 29.831002649032378 31.203035817451998 19.667889248742444 72-73 19.570236126084765 29.312092925692546 30.9067442377275 20.21092671049519 74-75 18.79292274831344 30.58936943033422 30.730594474325024 19.887113347027316 76-77 19.21001652469192 29.723369942965565 30.773632858398685 20.29298067394383 78-79 18.8150978326651 29.582860998944106 31.29995645250479 20.302084715886007 80-81 18.77482566863801 30.42422599859018 30.80648690447922 19.99446142829259 82-83 18.718748989897733 30.15136615554456 30.628818186564956 20.50106666799275 84-85 18.64502706452471 29.79882492472407 30.460799329665058 21.09534868108616 86-87 18.77388705774852 29.975058158779294 30.62550408160411 20.62555070186807 88-89 19.18407345396431 29.32318581892424 30.44527829466062 21.04746243245083 90-91 18.61057755067788 29.34670446139405 30.981948044682316 21.060769943245745 92-93 18.735287151903414 30.11571983318342 30.719898452986953 20.429094561926213 94-95 18.350721181401795 29.767391586050845 30.652857313736337 21.229029918811023 96-97 17.858775026767802 29.603743037800783 30.66912813125679 21.868353804174625 98-99 18.532883223137155 29.687005554882045 29.94205763772968 21.838053584251117 100-101 18.549940989187206 29.220904965083065 30.653141063040085 21.576012982689644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10794.0 1 7012.0 2 3427.0 3 3728.0 4 3408.5 5 3394.5 6 5559.0 7 10184.0 8 14085.0 9 15231.5 10 13154.5 11 9801.5 12 8071.5 13 7182.5 14 6532.5 15 5937.5 16 5548.0 17 5514.5 18 5576.0 19 5781.5 20 6354.5 21 6951.0 22 7634.0 23 8458.5 24 9410.5 25 11051.5 26 13880.5 27 17054.0 28 19264.0 29 21565.5 30 24647.5 31 28125.0 32 32363.5 33 37437.0 34 42920.0 35 46500.5 36 49886.0 37 53680.0 38 53969.0 39 52469.5 40 51173.5 41 50358.0 42 50129.0 43 49942.0 44 49898.5 45 49995.5 46 52165.5 47 62673.0 48 83965.0 49 79742.5 50 53684.5 51 39895.0 52 32998.5 53 29479.0 54 26449.0 55 23426.5 56 20742.5 57 18487.0 58 16882.0 59 15671.0 60 14559.0 61 12891.0 62 10247.0 63 7031.0 64 4686.5 65 3501.0 66 2557.0 67 1871.0 68 1285.5 69 851.5 70 666.0 71 562.5 72 490.0 73 447.5 74 363.0 75 268.5 76 202.0 77 126.5 78 81.5 79 56.5 80 35.0 81 21.5 82 15.0 83 13.5 84 8.0 85 2.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02275061103417067 2 0.019021002667913185 3 0.0 4 0.0 5 1.2432027887524958E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7296083662574875E-4 18-19 0.001181042649314871 20-21 3.1080069718812396E-5 22-23 0.0 24-25 0.0 26-27 7.14841603532685E-4 28-29 0.018399401273536935 30-31 0.004879570945853545 32-33 0.00907538035789322 34-35 0.009665901682550654 36-37 0.10169398811995414 38-39 0.10501955557986709 40-41 0.1191299072322079 42-43 0.10368311258195816 44-45 0.09933190282132441 46-47 0.10045078533120165 48-49 0.10290611083898785 50-51 0.1013210272833284 52-53 0.07819745541253198 54-55 0.06856263379970014 56-57 0.039471688542891736 58-59 0.017591319460847816 60-61 0.009572661473394218 62-63 0.0022377650197544924 64-65 0.005780892967699105 66-67 0.4283144407949536 68-69 0.8153234689336055 70-71 0.9063569931400071 72-73 0.28230027325597296 74-75 0.15207478113414905 76-77 0.015291394301655699 78-79 0.009914542240301154 80-81 0.0018648041831287436 82-83 0.0 84-85 0.0 86-87 3.418807669069363E-4 88-89 7.770017429703099E-4 90-91 0.0036052880873822376 92-93 0.002175604880316868 94-95 0.22402514253319974 96-97 0.23257216170587316 98-99 0.01572651527771907 100-101 0.008982140148736781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1608748.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.01984248391592 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2377483722228 26.704281295368325 2 13.73414875438262 10.443403445845945 3 5.820355791978791 6.638670312341439 4 2.92886486846223 4.454199238224364 5 1.8314378892146634 3.481549003350848 6 1.1746721659704422 2.679651043226193 7 0.798953441044987 2.1263258786359107 8 0.5667274911108282 1.7237511954670837 9 0.4222834297624665 1.4449634534823075 >10 2.1964922585621194 15.072605913082764 >50 0.17174787304170688 4.559690700483101 >100 0.10241304855070456 7.288481855011122 >500 0.006660995621469428 1.761290467505026 >1k 0.00582837116878575 4.694875204981045 >5k 9.991493432204143E-4 2.5016668551179446 >10k+ 6.660995621469429E-4 4.4245941378765865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22020 1.3687662704164978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20159 1.253086250923078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14755 0.9171728574021537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12951 0.8050359658566786 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8966 0.5573278101977439 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7782 0.4837302051035961 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6720 0.4177161370208386 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5898 0.366620502403111 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5144 0.3197517572671419 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5003 0.3109871776064368 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4663 0.2898527301976444 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4443 0.2761774995213669 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4104 0.2551052122520121 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3479 0.21625512510349662 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3307 0.2055635811202252 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3060 0.19021002667913184 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2858 0.17765367851273164 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2668 0.16584325201958294 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2664 0.16559461146183244 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2557 0.15894347654200658 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2472 0.15365986468980847 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2439 0.15160858008836686 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.1476924913037965 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2316 0.143962882937539 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.14240887945159839 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2253 0.14004679415296867 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2000 0.12432027887524957 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1858 0.11549353907510686 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1827 0.11356657475254048 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1794 0.11151529015109886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.1080069718812396E-5 0.0 16-17 0.0 0.0 0.0 1.2432027887524958E-4 0.0 18-19 0.0 0.0 0.0 2.4864055775049917E-4 0.0 20-21 0.0 0.0 0.0 6.216013943762479E-4 0.0 22-23 0.0 0.0 0.0 0.0016161636253782443 0.0 24-25 0.0 0.0 0.0 0.0032323272507564886 0.0 26-27 0.0 0.0 0.0 0.005439012200792169 0.0 28-29 0.0 0.0 0.0 0.009665901682550654 0.0 30-31 0.0 0.0 0.0 0.01970476420172706 0.0 32-33 0.0 0.0 0.0 0.035679920037196625 0.0 34-35 0.0 0.0 0.0 0.05933185309321286 0.0 36-37 0.0 0.0 0.0 0.09641037626775605 0.0 38-39 0.0 0.0 0.0 0.147443850746046 0.0 40-41 0.0 0.0 0.0 0.20749054544279155 0.0 42-43 0.0 0.0 0.0 0.26418059260990534 0.0 44-45 0.0 0.0 0.0 0.3355715127540174 0.0 46-47 0.0 0.0 0.0 0.42290650866388024 0.0 48-49 0.0 0.0 0.0 0.5148724349618461 0.0 50-51 0.0 0.0 0.0 0.6147637790381091 0.0 52-53 0.0 0.0 0.0 0.70607702387198 0.0 54-55 0.0 0.0 0.0 0.8073048109461519 0.0 56-57 0.0 0.0 0.0 0.9285170828495203 0.0 58-59 0.0 0.0 0.0 1.0448187037373162 0.0 60-61 0.0 6.216013943762479E-5 0.0 1.1753239164866094 3.1080069718812396E-5 62-63 0.0 1.2432027887524958E-4 0.0 1.2944849037885362 6.216013943762479E-5 64-65 0.0 1.2432027887524958E-4 0.0 1.4082068788896707 6.216013943762479E-5 66-67 0.0 1.2432027887524958E-4 0.0 1.5292948305141638 6.216013943762479E-5 68-69 0.0 1.2432027887524958E-4 0.0 1.6794737273954654 6.216013943762479E-5 70-71 0.0 1.2432027887524958E-4 0.0 1.8783861735958647 6.216013943762479E-5 72-73 0.0 1.2432027887524958E-4 0.0 2.0992411490177454 6.216013943762479E-5 74-75 0.0 1.2432027887524958E-4 0.0 2.3365996414603156 6.216013943762479E-5 76-77 0.0 1.2432027887524958E-4 0.0 2.580422788404399 6.216013943762479E-5 78-79 0.0 1.2432027887524958E-4 0.0 2.878542817147247 6.216013943762479E-5 80-81 0.0 1.2432027887524958E-4 0.0 3.203640346406025 6.216013943762479E-5 82-83 0.0 1.2432027887524958E-4 0.0 3.5264379505056107 6.216013943762479E-5 84-85 0.0 1.2432027887524958E-4 0.0 3.863501306606131 6.216013943762479E-5 86-87 0.0 1.2432027887524958E-4 0.0 4.21368045212799 6.216013943762479E-5 88-89 0.0 1.2432027887524958E-4 0.0 4.5651649605780396 6.216013943762479E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 265 0.0 30.393827 9 CGGGAGT 645 0.0 24.974771 4 GTCGGGA 650 0.0 24.787281 2 CGCGCCG 80 1.6439317E-6 24.091404 66-67 GTACATG 14410 0.0 22.829367 1 TACATGG 14230 0.0 22.61144 2 CGCGATC 105 3.5319317E-8 22.569662 22-23 GGTACCG 105 0.0022295068 22.561245 5 GAGTACT 15130 0.0 22.327826 12-13 GTACCGA 150 3.0947194E-5 22.110018 6 AGTACTT 15250 0.0 21.686098 12-13 TCGGGAG 750 0.0 21.478304 3 CGTCACT 265 8.1490725E-10 21.46514 1 ACATGGG 14475 0.0 21.24264 3 GCAACGC 250 0.0 21.168653 68-69 TACCGAC 135 3.8630227E-4 21.057161 7 GTACTTT 16230 0.0 20.887524 14-15 CGCATAG 255 0.0 20.78474 66-67 CGATCGT 115 9.239557E-8 20.608044 24-25 CCTTTAG 545 0.0 20.004591 1 >>END_MODULE