FastQCFastQC Report
Fri 27 May 2016
SRR939488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600256
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161021.0062140057590785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121070.756566449368101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91150.569596364581667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82980.5185420332746761No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56510.3531309990401536No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55170.34475733882578785No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42560.26595744680851063No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37720.23571228603423452No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.19065699488081908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.1905945048792193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28900.18059610462326028No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26020.162598984162534No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22330.13954017357222845No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.13822788353863383No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19990.12491751319788832No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT19270.12041823308270676No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18570.11604393297072468No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18390.11491911294192929No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17540.10960746280595104No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.10748280275155976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG1102.318031E-630.8743485
GTACATG123300.026.5206661
TACATGG124350.026.0895542
CGTCACT3000.025.1151941
GAGTACT124050.024.86270112-13
ACATGGG128000.024.119613
AGTACTT127150.022.91420212-13
GTACTTT138450.022.6039514-15
ATGGGAG29500.022.5315465
CATGGGG31900.022.1179034
CATGGGA92350.021.9910324
CATGGGT12300.021.3172174
ACTTTTT164750.021.14738816-17
GTACAGG7150.021.0756871
GCGTACA2156.3105836E-721.0266741
GTCAACG4850.019.8304943
GTCTAAG4400.019.4071941
AGTACAT92200.019.1302492
GTGTAGC6850.019.0655481
TACTTTT154700.019.03329514-15
Produced by FastQC (version 0.11.5)