##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939488_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1600256 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87341650335946 31.0 31.0 31.0 28.0 34.0 2 31.081152640577507 31.0 31.0 34.0 28.0 34.0 3 31.059930411134218 31.0 31.0 34.0 27.0 34.0 4 34.96498372760358 35.0 35.0 37.0 32.0 37.0 5 34.578296222604386 35.0 35.0 37.0 30.0 37.0 6 34.67560440329547 35.0 35.0 37.0 31.0 37.0 7 34.52564777135658 35.0 35.0 37.0 30.0 37.0 8 34.45447228443449 35.0 35.0 37.0 30.0 37.0 9 35.87766582346825 37.0 35.0 39.0 30.0 39.0 10-11 35.68314413443849 37.0 35.0 39.0 30.0 39.0 12-13 35.66842617681171 37.0 35.0 39.0 30.0 39.0 14-15 36.323225158974566 38.0 34.5 40.0 30.0 41.0 16-17 36.30123680211166 38.0 34.5 40.0 30.0 41.0 18-19 36.44860509818429 38.0 35.0 40.0 30.0 41.0 20-21 36.38760017147257 38.0 34.5 40.0 30.0 41.0 22-23 36.22302962775956 38.0 34.0 40.0 29.5 41.0 24-25 36.03679942459206 38.0 34.0 40.0 29.0 41.0 26-27 35.512420512717966 38.0 34.0 40.0 27.0 41.0 28-29 35.298587226043836 38.0 33.5 40.0 27.0 41.0 30-31 34.939164421192615 37.0 33.0 39.5 27.0 40.5 32-33 34.55015072588385 37.0 33.0 40.0 25.5 41.0 34-35 34.6202773181291 37.0 33.0 40.0 25.5 41.0 36-37 34.3441211906095 37.0 33.0 40.0 24.5 41.0 38-39 34.07763382858742 37.0 33.0 40.0 24.0 41.0 40-41 33.88324399346105 37.0 33.0 40.0 24.0 41.0 42-43 33.649616061430166 37.0 33.0 40.0 23.5 41.0 44-45 33.15885239611662 36.5 32.0 40.0 20.5 41.0 46-47 32.573175166973286 36.0 31.0 39.0 18.0 41.0 48-49 32.07055308650616 36.0 30.5 39.0 13.5 40.5 50-51 31.80061440169573 36.0 30.0 39.0 9.0 40.0 52-53 31.67932693269077 35.5 30.0 39.0 9.0 40.0 54-55 31.3837929681251 35.0 30.0 39.0 8.0 40.0 56-57 30.945405610102384 35.0 28.5 39.0 7.5 40.0 58-59 30.550192594184928 35.0 28.0 38.0 5.5 40.0 60-61 30.18669419142937 35.0 28.0 38.0 2.0 40.0 62-63 30.035895506718923 34.0 28.0 38.0 2.0 40.0 64-65 29.589718145096786 34.0 27.0 38.0 2.0 40.0 66-67 28.750645834166534 33.5 26.0 37.5 2.0 39.5 68-69 25.441625277455607 31.0 15.0 36.0 2.0 39.0 70-71 24.16653272976324 29.0 10.5 35.0 2.0 38.0 72-73 25.153417015777475 30.0 16.0 35.0 2.0 38.0 74-75 25.872149831027038 31.0 20.5 35.0 2.0 37.5 76-77 25.48758854833227 30.5 21.0 34.0 2.0 36.0 78-79 26.55432662024076 32.0 23.5 35.0 2.0 37.0 80-81 26.537918620520717 32.0 24.0 35.0 2.0 36.5 82-83 26.208673174792033 32.0 23.5 35.0 2.0 36.0 84-85 25.829399483582627 32.0 22.5 35.0 2.0 36.0 86-87 25.37450945348744 31.5 20.0 34.0 2.0 35.0 88-89 24.840084961406177 31.0 19.0 34.0 2.0 35.0 90-91 24.202813174892015 31.0 15.0 34.0 2.0 35.0 92-93 23.671421322588387 31.0 7.5 34.0 2.0 35.0 94-95 22.855404072848344 30.0 2.0 34.0 2.0 35.0 96-97 22.182614219224924 29.5 2.0 34.0 2.0 35.0 98-99 21.376654110342344 29.0 2.0 34.0 2.0 35.0 100-101 20.06136049482083 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1386970057687229 1101 2 0.14627399449621592 1101 3 0.16170807194289694 1101 4 0.3197554041934012 1101 5 0.547708560352703 1101 6 0.43615151577143507 1101 7 0.5430543840354076 1101 8 0.6425527872512404 1101 9 0.5565825783355507 1101 10-11 0.6240470573817589 1101 12-13 0.6451418502800195 1101 14-15 0.687766100326975 1101 16-17 0.6604586460202739 1101 18-19 0.5991128358300557 1101 20-21 0.6752156899210391 1101 22-23 0.6938807033424865 1101 24-25 0.6864864462616396 1101 26-27 0.8326229121116384 1101 28-29 0.7937869053984059 1101 30-31 0.5803551991751377 1101 32-33 0.9533060470875014 1101 34-35 0.821793525930488 1101 36-37 0.7380738842860879 1101 38-39 0.8539448457799921 1101 40-41 0.9125940663984267 1101 42-43 0.319014271077414 1101 44-45 0.742336028432355 1101 46-47 0.7521112754592139 1101 48-49 0.7985657272394349 1101 50-51 0.25225297754372633 1101 52-53 -0.14207698866714935 1101 54-55 0.1825453941010977 1101 56-57 0.3422568169184359 1101 58-59 0.36827019954164086 1101 60-61 0.32579318344254204 1101 62-63 0.17266757609109007 1101 64-65 0.15580372029948464 1101 66-67 -0.2149662209933112 1101 68-69 -6.605564176551024 1101 70-71 -7.303585229942058 1101 72-73 -4.621502164673938 1101 74-75 -2.6468627388741943 1101 76-77 -0.31540654275276125 1101 78-79 -0.11058616792826825 1101 80-81 -0.24847917423423027 1101 82-83 -0.3272405837686705 1101 84-85 -0.28325994524469067 1101 86-87 -0.3364311197519818 1101 88-89 -0.3295031815957472 1101 90-91 -0.5797530423022685 1101 92-93 -0.7148416627843481 1101 94-95 -0.5034316152304434 1101 96-97 -0.6492384999135368 1101 98-99 -0.49481078759271213 1101 100-101 -0.5037689358724471 1102 1 0.34643603762835795 1102 2 0.32777070363111704 1102 3 0.37344577279282376 1102 4 0.3871208509558315 1102 5 0.48309819088471784 1102 6 0.46320598001182844 1102 7 0.4408260707518181 1102 8 0.5714960445027017 1102 9 0.601165763622248 1102 10-11 0.5700072173285164 1102 12-13 0.57573336001726 1102 14-15 0.7768577426012016 1102 16-17 0.696428337350099 1102 18-19 0.4102194350438637 1102 20-21 0.7171410856990335 1102 22-23 0.6866546178081947 1102 24-25 0.7072778330496732 1102 26-27 0.6422208456626706 1102 28-29 0.44542613408955845 1102 30-31 0.5759913484963803 1102 32-33 0.7365643701280717 1102 34-35 0.5963001781590833 1102 36-37 0.7198119799270231 1102 38-39 0.7110206287351701 1102 40-41 0.6257530149007522 1102 42-43 0.7743366179408042 1102 44-45 0.7982567656763777 1102 46-47 0.776192117379253 1102 48-49 0.8293566064390205 1102 50-51 0.8436513273172785 1102 52-53 0.7036527825232604 1102 54-55 0.3557909710317695 1102 56-57 0.4576979394784857 1102 58-59 0.5963776278165724 1102 60-61 0.7620344345916656 1102 62-63 0.5917974566950086 1102 64-65 0.3404051787702933 1102 66-67 0.34594016277739215 1102 68-69 -6.213779213576782 1102 70-71 -7.022874718913286 1102 72-73 -1.1398317474926323 1102 74-75 0.46130435924265 1102 76-77 0.9148426267761387 1102 78-79 0.5009380637802181 1102 80-81 0.3407844841249066 1102 82-83 0.35356524109075593 1102 84-85 -0.14436964638789362 1102 86-87 0.11791411935037743 1102 88-89 -0.010142630241897876 1102 90-91 0.01735567750326794 1102 92-93 0.10913089360026973 1102 94-95 0.007477306482499557 1102 96-97 -0.37564832682587834 1102 98-99 -0.3429219945816371 1102 100-101 -0.05003562284265328 1103 1 0.2640471574780179 1103 2 0.24709549894210525 1103 3 0.27060224893152807 1103 4 0.2971432308398789 1103 5 0.3823555981848088 1103 6 0.3272203590643272 1103 7 0.45771998568618244 1103 8 0.5079355180925091 1103 9 0.6572654088371621 1103 10-11 0.6364560641619903 1103 12-13 0.5526033405256925 1103 14-15 0.7206822099866272 1103 16-17 0.6815976398391186 1103 18-19 0.6193388928490862 1103 20-21 0.6408296364520609 1103 22-23 0.6283956311303385 1103 24-25 0.7032512016315593 1103 26-27 0.7105963126001456 1103 28-29 0.6610972040650935 1103 30-31 0.5976344348783158 1103 32-33 0.7457467454864712 1103 34-35 0.8964481815845886 1103 36-37 0.924383252912861 1103 38-39 1.1634442883107354 1103 40-41 0.9729967385528795 1103 42-43 0.7112475132557492 1103 44-45 0.8132682131756397 1103 46-47 0.8903456233311289 1103 48-49 1.0244313762242427 1103 50-51 0.7417327098845199 1103 52-53 0.6703202770524648 1103 54-55 0.5115178941334548 1103 56-57 0.7178408653470711 1103 58-59 0.7398655230864399 1103 60-61 0.5803245856949779 1103 62-63 0.27986161052290726 1103 64-65 0.4061379245051917 1103 66-67 0.26070343548359176 1103 68-69 -6.605211020239604 1103 70-71 -7.250266022861183 1103 72-73 -0.80185502514761 1103 74-75 0.6502184519466958 1103 76-77 0.9266566851698848 1103 78-79 0.5468582191976132 1103 80-81 0.5517818101288974 1103 82-83 0.5386210344268321 1103 84-85 0.19171403027843326 1103 86-87 0.16485392546497124 1103 88-89 0.35220498555997537 1103 90-91 0.3081258380258127 1103 92-93 0.3255304826125105 1103 94-95 0.4100760589856307 1103 96-97 0.5527567407966956 1103 98-99 0.47305060132853427 1103 100-101 0.5533009391545924 1104 1 0.30349906958286255 1104 2 0.4011480183054772 1104 3 0.399413212136146 1104 4 0.31786862568752383 1104 5 0.5015025066457355 1104 6 0.4820218547262982 1104 7 0.49124096998269806 1104 8 0.6512425634126586 1104 9 0.7047676859059493 1104 10-11 0.5729180086310421 1104 12-13 0.4781443967842094 1104 14-15 0.6092824591832553 1104 16-17 0.7178661766257619 1104 18-19 0.5230167733637074 1104 20-21 0.6131371043334966 1104 22-23 0.5928515066308861 1104 24-25 0.5571715877115082 1104 26-27 0.7950632072449437 1104 28-29 0.6073882191826669 1104 30-31 0.678072831653104 1104 32-33 0.7730783524068343 1104 34-35 0.65717933917945 1104 36-37 0.9580529775728976 1104 38-39 0.9018414850597196 1104 40-41 0.9012333559890493 1104 42-43 1.0985784209306289 1104 44-45 0.9947242231000999 1104 46-47 1.2043402982151292 1104 48-49 1.1992462118020981 1104 50-51 1.0364814064849277 1104 52-53 0.972576067076286 1104 54-55 0.9224108125248769 1104 56-57 0.9790541105031423 1104 58-59 1.0720826255457965 1104 60-61 0.8908818059771946 1104 62-63 0.6504675911212559 1104 64-65 0.7085753073740371 1104 66-67 0.35920949149194925 1104 68-69 -6.165603526015911 1104 70-71 -4.023971800859179 1104 72-73 0.5389667148765369 1104 74-75 1.3406107889396175 1104 76-77 1.6923108926082868 1104 78-79 1.0962131953840846 1104 80-81 0.847315952296352 1104 82-83 0.6622660202640915 1104 84-85 0.65082354116225 1104 86-87 0.5095130764483464 1104 88-89 0.756329840662449 1104 90-91 0.7074081167944932 1104 92-93 0.8768398800497756 1104 94-95 1.017212070067469 1104 96-97 0.5792344845967676 1104 98-99 0.5952497765805518 1104 100-101 0.7651025451285598 1105 1 0.0841837964670944 1105 2 0.16797954292782435 1105 3 0.24647119438233744 1105 4 0.09222021563371641 1105 5 0.3783791614390424 1105 6 0.2363472369025672 1105 7 0.36702909776513337 1105 8 0.32216941879808303 1105 9 0.2203340499934754 1105 10-11 0.3848738979227804 1105 12-13 0.4176394693812071 1105 14-15 0.4326859121547315 1105 16-17 0.3299652063026386 1105 18-19 0.37489153347215876 1105 20-21 0.44394480237695433 1105 22-23 0.36700079312924316 1105 24-25 0.33934608696463897 1105 26-27 0.46228503694822365 1105 28-29 0.2607320934831634 1105 30-31 0.3674178574330469 1105 32-33 0.42116172587488876 1105 34-35 0.32111175149221793 1105 36-37 0.7065904030995611 1105 38-39 0.29290291196522844 1105 40-41 0.31476849567010845 1105 42-43 0.2277158625926674 1105 44-45 0.21425860735197233 1105 46-47 0.4936543978486583 1105 48-49 0.5153468770198373 1105 50-51 0.3776997866393863 1105 52-53 0.28627170490054965 1105 54-55 0.26754660642228245 1105 56-57 0.4362354550693084 1105 58-59 0.6716645113806443 1105 60-61 0.8693661314327166 1105 62-63 0.5022433386661085 1105 64-65 0.40974438905084654 1105 66-67 0.48623191478166206 1105 68-69 -2.557116010155564 1105 70-71 -2.3283293926780537 1105 72-73 0.8298653211219076 1105 74-75 1.2193326223100307 1105 76-77 1.119001257096759 1105 78-79 0.5746315124638777 1105 80-81 0.5152672356703114 1105 82-83 0.39857142146055935 1105 84-85 0.38913150201683777 1105 86-87 0.5689606474623545 1105 88-89 0.7590050134454813 1105 90-91 0.5161949928625447 1105 92-93 0.36665350417852594 1105 94-95 0.34351408527873417 1105 96-97 0.6356847864017325 1105 98-99 0.38860047174653545 1105 100-101 0.4661276918749593 1106 1 0.025589294017709818 1106 2 0.19364615210014335 1106 3 0.20103418909075899 1106 4 0.14530218536482664 1106 5 0.22890466815518096 1106 6 0.16186540994208087 1106 7 0.31135925296209876 1106 8 0.3674704520596137 1106 9 0.20019602579297668 1106 10-11 0.3910346584093034 1106 12-13 0.2521726824380437 1106 14-15 0.23394280360749775 1106 16-17 0.34681739715197324 1106 18-19 0.18035804973025904 1106 20-21 0.3014860881435837 1106 22-23 0.1321223307211028 1106 24-25 0.23443658390463895 1106 26-27 0.3512229569035057 1106 28-29 0.32961278558079243 1106 30-31 0.4540191358219303 1106 32-33 0.4543359110813796 1106 34-35 0.3567575116199322 1106 36-37 0.45858088253945795 1106 38-39 0.18141827995212623 1106 40-41 0.2404641877488558 1106 42-43 0.20430601141909221 1106 44-45 0.4148124730493734 1106 46-47 0.4066760488764487 1106 48-49 0.5752189500015703 1106 50-51 0.28488109275145135 1106 52-53 0.12344488416582422 1106 54-55 0.29634623778699876 1106 56-57 0.672963703636249 1106 58-59 0.8291379055572641 1106 60-61 0.780758006600319 1106 62-63 0.3009509119304852 1106 64-65 0.19860406213883408 1106 66-67 0.4142603770009501 1106 68-69 -2.7166708465421827 1106 70-71 -3.2810855646121695 1106 72-73 0.7663671699638144 1106 74-75 1.1281542484096079 1106 76-77 1.117644684296959 1106 78-79 0.9368594267662438 1106 80-81 0.748454073829599 1106 82-83 0.4472583785953752 1106 84-85 0.24944636539642318 1106 86-87 0.5196382099370958 1106 88-89 0.4766225820389671 1106 90-91 0.7422127686874695 1106 92-93 0.6439264403308442 1106 94-95 1.0389656949735375 1106 96-97 1.0148215465659547 1106 98-99 1.075289911629902 1106 100-101 0.9532832136613294 1107 1 0.11868682645621931 1107 2 0.19008810458939607 1107 3 0.18975860309420156 1107 4 0.16927775952971302 1107 5 0.36337165120959725 1107 6 0.15112685051420982 1107 7 0.07365104835299974 1107 8 0.12034883558992249 1107 9 0.41687934444626507 1107 10-11 0.2381421426008714 1107 12-13 0.17300086680182858 1107 14-15 0.07155717646300985 1107 16-17 0.05618561858833715 1107 18-19 0.12247207420867312 1107 20-21 0.37951286059418265 1107 22-23 0.08269846754784993 1107 24-25 0.14561324395432962 1107 26-27 0.5357318438058414 1107 28-29 0.4318151203646039 1107 30-31 0.33023056784895743 1107 32-33 0.27126013577802865 1107 34-35 0.24425685430466615 1107 36-37 0.4023076300120749 1107 38-39 0.5434856021812067 1107 40-41 0.2814509165537231 1107 42-43 0.13571943642599393 1107 44-45 0.5185601560130735 1107 46-47 0.6845734945493405 1107 48-49 0.7196199386037208 1107 50-51 0.9596137297688223 1107 52-53 0.5949045841671357 1107 54-55 0.5714965986530807 1107 56-57 0.8844200513297302 1107 58-59 1.1565773668216757 1107 60-61 0.9109287771052692 1107 62-63 0.7387838421104576 1107 64-65 0.5229216045685341 1107 66-67 0.8358305059662072 1107 68-69 -2.181635614444165 1107 70-71 -2.4221353956821474 1107 72-73 1.5340762290827499 1107 74-75 1.6843006390915072 1107 76-77 1.143099382129133 1107 78-79 1.0131945983912587 1107 80-81 0.8882446531872716 1107 82-83 0.5872270903889252 1107 84-85 0.48837076756819187 1107 86-87 0.48033864507980084 1107 88-89 0.6703072613348482 1107 90-91 0.7536354289737979 1107 92-93 0.6275316830773683 1107 94-95 0.9677022035777938 1107 96-97 0.5739850142983478 1107 98-99 0.7594827942202507 1107 100-101 1.0177456283746729 1108 1 -0.02967079087132518 1108 2 -0.03140153920026023 1108 3 0.06888924963018184 1108 4 -0.03717339514377471 1108 5 -0.0590277968199544 1108 6 -0.2405103912569686 1108 7 0.014628766604552368 1108 8 0.30621676854174495 1108 9 0.3320686784289748 1108 10-11 0.24097403512897841 1108 12-13 -0.04643799705899454 1108 14-15 0.020260620866871193 1108 16-17 0.10085886696150936 1108 18-19 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94-95 0.8945904876109374 2316 96-97 1.2582007810197169 2316 98-99 1.1027362065406265 2316 100-101 1.2053967311245444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 32.0 4 75.0 5 247.0 6 598.0 7 1262.0 8 2302.0 9 4013.0 10 6353.0 11 9061.0 12 11619.0 13 14387.0 14 17739.0 15 22596.0 16 26898.0 17 29671.0 18 25168.0 19 19537.0 20 19046.0 21 19506.0 22 20564.0 23 22852.0 24 26669.0 25 32183.0 26 39701.0 27 49187.0 28 59016.0 29 69695.0 30 81659.0 31 94420.0 32 108369.0 33 124906.0 34 143829.0 35 159162.0 36 152577.0 37 125821.0 38 56002.0 39 3531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14165708285414 21.6225186259313 15.975923796189809 19.259900495024752 2 15.100205316991309 24.01608785192176 39.169606835149516 21.71409999593741 3 19.778509192643387 28.76443449042554 31.782281110353587 19.674775206577486 4 11.653815768980328 19.473253932362184 38.66221811060216 30.21071218805533 5 11.124782534794434 41.15991441369381 33.746600543912976 13.968702507598785 6 26.499822528395455 39.416630839065746 18.18327817549192 15.900268457046874 7 24.841900295952648 37.255976543753 20.9230273156295 16.979095844664855 8 25.367816149416093 34.271829007358825 19.981615441529353 20.37873940169573 9 26.241988781794912 18.25920352743561 22.12127309630459 33.37753459446488 10-11 23.694968111268395 28.725794405957146 26.620888134766997 20.958349348007463 12-13 26.719131189009758 27.047484902415615 27.544967805151177 18.688416103423453 14-15 22.98850933850584 27.625986092225247 29.154304061350185 20.231200507918736 16-17 18.630574591756677 31.328384702174457 29.75752707171203 20.283513634356837 18-19 18.073974506547085 29.362463708886217 34.18919746490245 18.374364319664252 20-21 20.02173402255639 27.442078017517197 33.699326857702765 18.836861102223644 22-23 19.124169467005988 27.798644276450123 33.5487055948983 19.528480661645585 24-25 19.257209509713032 28.079412796748898 34.245503127586595 18.41787456595147 26-27 19.5511301268313 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19.092675712394023 52-53 19.03241942038953 30.37677806608935 31.339075249320036 19.251727264201083 54-55 18.434064937874258 29.598286462377573 31.744022186961157 20.223626412787013 56-57 19.528507774705588 30.115908791872663 30.521731765547145 19.8338516678746 58-59 19.748008276593996 29.424785467645997 30.80353027172692 20.023675984033087 60-61 19.416411440028224 29.44345124538412 31.779411686583252 19.360725628004403 62-63 19.992463573940714 29.292888263422462 31.3674988814094 19.347149281227424 64-65 19.511986301369863 29.98406409359064 31.2743100689931 19.229639536046395 66-67 19.220909272982176 29.671414980973367 31.057198828359706 20.050476917684758 68-69 19.516698319812814 29.764669297539363 30.99064380421926 19.727988578428565 70-71 19.541666640395604 29.75450007534541 31.010057193153468 19.693776091105523 72-73 19.686230263172337 29.235639008949484 30.863693080276295 20.214437647601883 74-75 19.00792291167004 30.542474359327056 30.554822647322894 19.894780081680015 76-77 18.739245918145002 29.800233928674295 31.099521122456903 20.3609990307238 78-79 18.829103540601196 29.65424592775551 31.26486291896635 20.251787612676942 80-81 18.782474364995224 30.423798958195658 30.807156210346864 19.986570466462254 82-83 18.744012583183302 30.169425539187777 30.56661819449817 20.519943683130748 84-85 18.669067535528736 29.876507104497165 30.358680559461188 21.095744800512918 86-87 18.78795548139955 29.930135043087557 30.572355223936558 20.709554251576336 88-89 19.17730406042552 29.343709205618367 30.393200816621892 21.085785917334217 90-91 18.62619224309651 29.432363394914656 30.866382845760263 21.075061516228565 92-93 18.743559346501378 30.15056990214269 30.625108190480237 20.480762560875696 94-95 18.50201778452075 29.82596960284072 30.42061098252629 21.251401630112237 96-97 18.07273653595803 29.644975315336996 30.42641407408681 21.855874074618164 98-99 18.572391047968207 29.69902608623915 29.819085659787042 21.909497206005604 100-101 18.54845715449954 29.167789018307406 30.61873188743193 21.66502193976112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10341.0 1 6958.0 2 3600.5 3 3702.5 4 3325.0 5 3317.0 6 5545.0 7 10211.0 8 14025.0 9 14886.0 10 12667.5 11 9503.0 12 7953.5 13 6989.0 14 6374.5 15 5877.5 16 5510.5 17 5535.5 18 5682.0 19 5875.5 20 6260.0 21 6784.0 22 7448.5 23 8342.5 24 9387.5 25 11060.5 26 13889.0 27 16897.0 28 19288.0 29 21685.5 30 24834.5 31 28303.0 32 32128.5 33 37058.0 34 42506.0 35 46356.0 36 50126.0 37 53773.0 38 53763.0 39 52279.0 40 50917.5 41 49956.0 42 49905.5 43 49652.0 44 49394.0 45 49819.0 46 51876.0 47 61726.0 48 83300.5 49 79410.0 50 53475.0 51 40006.5 52 33106.5 53 29352.5 54 26290.5 55 23391.5 56 20534.5 57 18230.0 58 16800.5 59 15602.5 60 14334.5 61 12774.0 62 10214.5 63 7039.0 64 4672.0 65 3421.5 66 2596.0 67 1942.0 68 1293.5 69 857.0 70 694.5 71 582.5 72 497.5 73 456.0 74 380.0 75 291.0 76 215.5 77 127.5 78 82.0 79 56.0 80 37.5 81 29.0 82 17.5 83 9.5 84 5.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020996640537513996 2 0.018184590465525518 3 5.624100143976964E-4 4 0.0959221524556071 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01006089025755879 12-13 0.0 14-15 0.0 16-17 9.685950247960327E-4 18-19 1.2498000319948808E-4 20-21 0.0 22-23 2.1871500559910416E-4 24-25 0.0037806450967845144 26-27 0.0037181550951847706 28-29 0.0012498000319948808 30-31 1.8747000479923213E-4 32-33 7.186350183970565E-4 34-35 0.002874540073588226 36-37 0.08523636218205087 38-39 0.09698448248280275 40-41 0.07723764197728364 42-43 0.0801746720524716 44-45 0.07942479203327467 46-47 0.07833121700527916 48-49 0.07317579187330027 50-51 0.07239466685330348 52-53 0.0566159414493681 54-55 0.04736742121260598 56-57 0.02627704567269237 58-59 0.020153025515917453 60-61 0.012966675331946889 62-63 0.0017497200447928333 64-65 0.005999040153575428 66-67 0.15597504399296114 68-69 0.7300706886898096 70-71 0.8890452527595585 72-73 0.3628481942889138 74-75 0.052741561350183964 76-77 0.199218125099984 78-79 0.013560330347144458 80-81 0.0032182350823868186 82-83 0.001562250039993601 84-85 1.2498000319948808E-4 86-87 0.001624740041593345 88-89 3.124500079987202E-4 90-91 0.004280565109582467 92-93 0.004311810110382339 94-95 0.07655025195968646 96-97 0.03615046592545192 98-99 0.013560330347144458 100-101 0.0120605703087506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1600256.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.16718109608228 #Duplication Level Percentage of deduplicated Percentage of total 1 71.4868195161976 27.999372059738604 2 13.276014850604156 10.399681557757816 3 5.460380430144722 6.416031274829461 4 2.9016907829653618 4.546041935249485 5 1.6939043306572599 3.317272883914547 6 1.090350889871141 2.5623582457114464 7 0.7563995659326478 2.0738227145917434 8 0.5309446988993257 1.6636485739035816 9 0.3999015574923612 1.4096715050617805 >10 2.1210035461289674 14.808521832740507 >50 0.17118314482382554 4.645203234626489 >100 0.09721582386880648 7.010896233068677 >500 0.007340101248527951 2.0238093932362533 >1k 0.005382740915587164 4.6779269963869226 >5k 8.155668053919945E-4 2.121492471784717 >10k+ 6.524534443135956E-4 4.324249087397848 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21521 1.3448473244280916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19281 1.2048697208446648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14277 0.8921697528395457 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12607 0.7878114501679732 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8366 0.5227913533834586 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7523 0.47011228203487443 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6498 0.4060600303951368 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5584 0.34894416893297076 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5236 0.3271976483762598 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4652 0.2907034874420093 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4568 0.28545432730763076 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4312 0.2694568868980963 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3838 0.23983662613981765 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3410 0.21309090545512718 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3230 0.20184270516717326 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2907 0.18165843465045592 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2771 0.17315979443289076 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2548 0.15922452407614782 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2527 0.15791223404255317 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2504 0.15647496400575908 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2437 0.15228813389857623 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2409 0.1505384138537834 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2338 0.14610162374020158 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 0.14466435370340747 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2238 0.13985262358022715 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2118 0.13235382338825788 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1915 0.11966835306350984 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1772 0.11073228283474644 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1729 0.10804521276595745 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1682 0.10510818269076948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.249000159974404E-5 0.0 14-15 0.0 0.0 0.0 9.373500239961605E-5 0.0 16-17 0.0 0.0 0.0 3.4369500879859226E-4 0.0 18-19 0.0 0.0 0.0 4.999200127979524E-4 0.0 20-21 0.0 0.0 0.0 7.186350183970565E-4 0.0 22-23 0.0 0.0 0.0 0.001593495040793473 0.0 24-25 0.0 0.0 0.0 0.0026245800671892494 0.0 26-27 0.0 0.0 0.0 0.004905465125579907 0.0 28-29 0.0 0.0 0.0 0.008404905215165574 0.0 30-31 0.0 0.0 0.0 0.017590935450327947 0.0 32-33 0.0 0.0 0.0 0.03236982082866741 0.0 34-35 0.0 0.0 0.0 0.055334896416573354 0.0 36-37 0.0 0.0 0.0 0.09498480243161095 0.0 38-39 0.0 0.0 0.0 0.14247720364741642 0.0 40-41 0.0 0.0 0.0 0.2019989301711726 0.0 42-43 0.0 0.0 0.0 0.25998965165573507 0.0 44-45 0.0 0.0 0.0 0.3287911434170533 0.0 46-47 0.0 0.0 0.0 0.4137775455927052 0.0 48-49 0.0 0.0 0.0 0.5061690129579267 0.0 50-51 0.0 0.0 0.0 0.604965705487122 0.0 52-53 0.0 0.0 0.0 0.6996068129099344 0.0 54-55 0.0 0.0 0.0 0.7997470404735243 0.0 56-57 0.0 0.0 0.0 0.9204777235642297 0.0 58-59 0.0 0.0 0.0 1.0458326667733162 0.0 60-61 0.0 0.0 0.0 1.182654525275956 0.0 62-63 0.0 6.249000159974404E-5 0.0 1.3067284234522476 0.0 64-65 6.249000159974404E-5 6.249000159974404E-5 0.0 1.4340205567109263 0.0 66-67 6.249000159974404E-5 6.249000159974404E-5 0.0 1.5716860302351623 0.0 68-69 6.249000159974404E-5 6.249000159974404E-5 0.0 1.7255364141737322 0.0 70-71 6.249000159974404E-5 6.249000159974404E-5 0.0 1.9207551791713326 0.0 72-73 6.249000159974404E-5 6.249000159974404E-5 0.0 2.1415635998240283 0.0 74-75 6.249000159974404E-5 6.249000159974404E-5 0.0 2.3684647956326987 0.0 76-77 6.249000159974404E-5 6.249000159974404E-5 0.0 2.6120508218685012 0.0 78-79 6.249000159974404E-5 6.249000159974404E-5 0.0 2.9046290093585023 0.0 80-81 6.249000159974404E-5 6.249000159974404E-5 0.0 3.2337950927851544 0.0 82-83 6.249000159974404E-5 6.249000159974404E-5 0.0 3.5572745860662294 0.0 84-85 6.249000159974404E-5 6.249000159974404E-5 0.0 3.905218914973604 0.0 86-87 6.249000159974404E-5 6.249000159974404E-5 0.0 4.259443489041754 0.0 88-89 6.249000159974404E-5 6.249000159974404E-5 0.0 4.629603013517837 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 210 0.0 33.897278 1 CATTCCG 310 0.0 32.116596 9 TATAACG 100 4.993011E-5 28.467478 2 CGTCGAT 35 0.007957505 27.44145 64-65 TAGCCCG 110 9.601609E-5 25.860117 5 GTACATG 13880 0.0 24.206758 1 TACATGG 13730 0.0 23.878403 2 CGCATAG 340 0.0 23.308378 66-67 ACATGGG 13720 0.0 22.785637 3 GAGTACT 14615 0.0 22.58982 12-13 AGCCCGT 130 2.980087E-4 21.881636 6 AACGCAT 405 0.0 21.343351 64-65 GTACTTT 15630 0.0 21.10769 14-15 TGCGTCG 80 4.669352E-5 20.962746 62-63 CATGGGT 1090 0.0 20.894218 4 TAACGCA 160 5.0337174E-5 20.758186 4 CATGGGA 9995 0.0 20.697365 4 AGTACTT 14770 0.0 20.683924 12-13 CGTACAC 140 4.8955437E-4 20.330736 3 CCTTTAG 540 0.0 20.21282 1 >>END_MODULE