FastQCFastQC Report
Fri 27 May 2016
SRR939487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138570.2744780569084901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105600.2091714137947359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68740.13615949795691426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150300.038.322061
GTATCAA240650.035.1079181
TATCAAC297800.028.5827542
ATCAACG301950.028.1536833
TCAACGC305400.027.7817844
CAACGCA308550.027.4492175
AACGCAG309850.027.0801416
GTACATG211700.026.0203381
TACATGG214700.024.9494862
ACGCAGA343700.024.0586557
CGCAGAG349800.023.5529738
GAGTACT167700.023.16512-13
ACATGGG228450.022.9188583
GCAGAGT351050.021.9958119
GTACTTT181800.021.4250714-15
AGTACTT176400.020.66393312-13
AGAGTAC287800.020.6094210-11
CATGGGA135100.019.7165094
CAGAGTA341750.019.1116810-11
GTGTAGC33450.018.932051
Produced by FastQC (version 0.11.5)