FastQCFastQC Report
Fri 27 May 2016
SRR939487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048491
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198180.3925529430477344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171650.3400025869116138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113260.22434426445446767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171550.036.4157331
GTATCAA263950.031.8025021
ATCAACG327900.025.373463
TCAACGC329350.025.2761384
TATCAAC330500.025.2497842
CAACGCA335350.024.7673875
AACGCAG343200.024.2284976
GTACATG236000.023.5988181
TACATGG239150.022.6290052
ACGCAGA387750.021.3959227
CGCAGAG389650.021.2186328
ACATGGG244950.021.0256163
GAGTACT194050.021.01037812-13
GTACTTT200650.020.24844214-15
ACCGTCG4450.020.2297368
GCAGAGT405250.019.840639
AGTACTT200300.019.58601212-13
AGAGTAC333450.019.58010910-11
CATGGGA150350.018.2776854
ACTTTTT239950.017.3862216-17
Produced by FastQC (version 0.11.5)