##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5048491 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.000643558639602 31.0 31.0 31.0 30.0 34.0 2 31.26869870620746 31.0 31.0 34.0 30.0 34.0 3 31.263211125859193 31.0 31.0 34.0 28.0 34.0 4 35.19747722636328 35.0 35.0 37.0 33.0 37.0 5 34.82245328356532 35.0 35.0 37.0 32.0 37.0 6 34.76705118420534 35.0 35.0 37.0 32.0 37.0 7 34.639251411956565 35.0 35.0 37.0 30.0 37.0 8 34.657686227429146 35.0 35.0 37.0 30.0 37.0 9 36.0113528973311 37.0 35.0 39.0 30.0 39.0 10-11 35.865698086814454 37.0 35.0 39.0 30.0 39.0 12-13 35.82841288614756 37.0 35.0 39.0 30.0 39.0 14-15 36.70548942248288 38.0 36.0 40.0 31.0 41.0 16-17 36.714846178788875 38.0 36.0 40.0 31.0 41.0 18-19 36.67317749006584 38.0 35.0 40.0 30.0 41.0 20-21 36.56623593069692 38.0 35.0 40.0 30.0 41.0 22-23 36.42962134625971 38.0 35.0 40.0 30.0 41.0 24-25 36.209120012296744 38.0 34.0 40.0 29.5 41.0 26-27 35.846030823864 38.0 34.0 40.0 28.0 41.0 28-29 35.56508558696054 38.0 34.0 40.0 27.0 41.0 30-31 35.37107781315248 38.0 34.0 40.0 27.0 41.0 32-33 35.18100705735635 38.0 33.5 40.0 27.0 41.0 34-35 35.14615743595462 38.0 33.5 40.0 27.0 41.0 36-37 35.16172783114796 38.0 33.5 40.0 26.5 41.0 38-39 35.14965630323992 38.0 34.0 40.0 27.0 41.0 40-41 34.94104119428954 38.0 33.0 40.0 25.5 41.0 42-43 34.64553526984598 38.0 33.0 40.0 25.0 41.0 44-45 34.47684852760953 38.0 33.0 40.0 24.5 41.0 46-47 34.171251568042806 37.5 33.0 40.0 24.0 41.0 48-49 33.87753756518532 37.0 33.0 40.0 23.5 41.0 50-51 33.62034813967183 37.0 32.0 40.0 23.0 41.0 52-53 33.25445415273593 37.0 31.5 40.0 21.5 41.0 54-55 33.03658568471252 37.0 31.0 39.5 20.5 41.0 56-57 32.65178793029442 36.0 31.0 39.0 19.0 40.0 58-59 32.221100621948224 36.0 30.5 39.0 18.0 40.0 60-61 31.941249573387374 35.0 30.0 39.0 17.5 40.0 62-63 31.553576306266564 35.0 30.0 38.5 14.0 40.0 64-65 31.125351813046713 35.0 29.0 38.0 12.0 40.0 66-67 29.483162097347503 33.5 26.5 37.5 7.5 39.5 68-69 25.8844247716793 30.5 17.0 36.0 4.5 39.0 70-71 25.286350119273266 29.5 16.0 35.0 4.0 38.0 72-73 27.068137984201616 31.0 22.5 35.0 4.0 38.0 74-75 27.65251062149066 32.0 25.0 35.0 2.0 38.0 76-77 27.661744568822645 31.5 25.5 34.5 2.0 36.5 78-79 28.497885902936144 33.0 27.0 35.0 2.0 37.0 80-81 28.34895902557814 33.0 26.5 35.0 2.0 37.0 82-83 27.941837570870188 33.0 26.0 35.0 2.0 36.0 84-85 27.611713381285618 33.0 26.0 35.0 2.0 36.0 86-87 27.16491977503773 32.0 25.5 35.0 2.0 35.5 88-89 26.683986165371 32.0 25.0 34.0 2.0 35.0 90-91 26.25436640374322 32.0 24.5 34.0 2.0 35.0 92-93 25.709532412754623 32.0 23.5 34.0 2.0 35.0 94-95 24.36221298601899 31.0 18.0 34.0 2.0 35.0 96-97 23.566777676735484 30.5 7.0 34.0 2.0 35.0 98-99 23.235915642911912 31.0 2.0 34.0 2.0 35.0 100-101 21.96484801101953 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3310770500650264 1101 2 0.25890083164446764 1101 3 0.3044772949099581 1101 4 0.3052262245328805 1101 5 0.43338316673084165 1101 6 0.5277089812216573 1101 7 0.46637640265227986 1101 8 0.5211532023272696 1101 9 0.5826121627702108 1101 10-11 0.7766889378367239 1101 12-13 0.7426922582483755 1101 14-15 0.8257178062073862 1101 16-17 0.7496143008196086 1101 18-19 0.7938137442918958 1101 20-21 0.8209269811728106 1101 22-23 0.802376469542537 1101 24-25 0.754457984799572 1101 26-27 0.8500317266930111 1101 28-29 0.7055935138986484 1101 30-31 0.783860525952818 1101 32-33 0.7518893581775927 1101 34-35 0.8683633759207794 1101 36-37 0.7367604179176865 1101 38-39 0.6746504530107913 1101 40-41 0.8546726820820894 1101 42-43 0.9337969083745108 1101 44-45 0.5964616231324626 1101 46-47 0.6093976179602834 1101 48-49 0.5795785802640339 1101 50-51 0.566695850137755 1101 52-53 0.7012576754114761 1101 54-55 0.5086901665663035 1101 56-57 0.406332988412629 1101 58-59 0.418757581157692 1101 60-61 0.26552067508535515 1101 62-63 0.2675347368565433 1101 64-65 -0.04868098511985863 1101 66-67 -2.9982146464863035 1101 68-69 -7.908288118775513 1101 70-71 -11.53327027833916 1101 72-73 -11.498797078949526 1101 74-75 -9.933115860442104 1101 76-77 -4.8738885327032975 1101 78-79 -1.8094840912201207 1101 80-81 -1.164135483037775 1101 82-83 -0.9028978650360067 1101 84-85 -1.0671715395220183 1101 86-87 -0.9774766917180138 1101 88-89 -0.8891454122752087 1101 90-91 -0.9402022454101946 1101 92-93 -1.553960078726103 1101 94-95 -5.172404092955524 1101 96-97 -2.1957441556985415 1101 98-99 -1.586299413204408 1101 100-101 -1.3059137689210587 1102 1 0.388964149848551 1102 2 0.20215399617297436 1102 3 0.3500176194666089 1102 4 0.21250008803385612 1102 5 0.36290454795115323 1102 6 0.45110502440895317 1102 7 0.4348775142554402 1102 8 0.5023711552240684 1102 9 0.6020553833397031 1102 10-11 0.5596999830584011 1102 12-13 0.5749768837314022 1102 14-15 0.5649413530869225 1102 16-17 0.6559386933717448 1102 18-19 0.6816842880445506 1102 20-21 0.774344598902708 1102 22-23 0.7261481986305753 1102 24-25 0.6809879283989133 1102 26-27 0.7520947396433542 1102 28-29 0.6849234835740248 1102 30-31 0.46256759323122054 1102 32-33 0.3873766708404318 1102 34-35 0.5474105836500698 1102 36-37 0.8177344981194636 1102 38-39 0.6701152469989182 1102 40-41 0.7290335664641248 1102 42-43 0.8548230675776622 1102 44-45 0.6286039825185981 1102 46-47 0.6474638443316962 1102 48-49 0.5424417339350214 1102 50-51 0.4979703385642722 1102 52-53 0.5829708480775011 1102 54-55 0.5229925035180614 1102 56-57 0.4317847949644573 1102 58-59 0.38752647784941274 1102 60-61 0.17777286850655116 1102 62-63 0.21335728947504506 1102 64-65 0.3226378831354957 1102 66-67 -2.5653303566197643 1102 68-69 -7.642287327502888 1102 70-71 -11.069134372770355 1102 72-73 -9.48218597370802 1102 74-75 -7.781301525144826 1102 76-77 -4.105483435963475 1102 78-79 -1.216691500184428 1102 80-81 -0.64961397256128 1102 82-83 -0.6197614975168477 1102 84-85 -0.5498122938835692 1102 86-87 -0.4408415560664629 1102 88-89 -0.42960063006625404 1102 90-91 -0.47921471132589843 1102 92-93 -0.9251137238628111 1102 94-95 -4.624654190178653 1102 96-97 -1.3087300746425505 1102 98-99 -0.6065729642426163 1102 100-101 -0.36701709968425433 1103 1 0.40709475129813555 1103 2 0.32874060659690585 1103 3 0.42452571420019325 1103 4 0.29771619724797915 1103 5 0.3870952659316629 1103 6 0.4928172024264015 1103 7 0.514360286637455 1103 8 0.4007864035219768 1103 9 0.8039624592750556 1103 10-11 0.7582897188684257 1103 12-13 0.6623425500777103 1103 14-15 0.841957874937286 1103 16-17 0.786835830065435 1103 18-19 0.6917772842713461 1103 20-21 0.8661418817793987 1103 22-23 0.8052716636816868 1103 24-25 0.8663292826636493 1103 26-27 0.8844984660526265 1103 28-29 0.9357670836120704 1103 30-31 0.925208924425931 1103 32-33 0.9561653008202953 1103 34-35 0.9096631686401295 1103 36-37 0.9784683570337265 1103 38-39 0.9070559493989663 1103 40-41 0.9645358095040351 1103 42-43 1.0733892796228375 1103 44-45 0.858526369948386 1103 46-47 0.8368787378324001 1103 48-49 0.7222964420227598 1103 50-51 0.6998768678167266 1103 52-53 0.8734915307313642 1103 54-55 0.8633446301691805 1103 56-57 0.8654765336019281 1103 58-59 0.8284530862178201 1103 60-61 0.667523794314036 1103 62-63 0.6494287387423263 1103 64-65 0.5563686002631805 1103 66-67 -2.660811120488834 1103 68-69 -7.564948303747329 1103 70-71 -11.522803134325866 1103 72-73 -9.154203435852963 1103 74-75 -7.465322981901664 1103 76-77 -3.7859873299857156 1103 78-79 -0.8423167004070358 1103 80-81 -0.21298189998802997 1103 82-83 0.025714851887869372 1103 84-85 0.007788607377847256 1103 86-87 0.09232043318138139 1103 88-89 0.24897695844990153 1103 90-91 0.16182516973777084 1103 92-93 0.2137591745514804 1103 94-95 1.117612023615905 1103 96-97 1.1013439380208112 1103 98-99 0.7248158863784688 1103 100-101 0.5942864984732061 1104 1 0.4076874176627143 1104 2 0.21264625168849705 1104 3 0.3355932486838853 1104 4 0.1990084724325598 1104 5 0.4098488079966387 1104 6 0.5750364845388205 1104 7 0.5257948098267633 1104 8 0.3656391678309774 1104 9 0.5746147113264684 1104 10-11 0.5927777924422486 1104 12-13 0.5364640509230014 1104 14-15 0.5952464160208777 1104 16-17 0.6110375579453446 1104 18-19 0.6886608890398165 1104 20-21 0.8047944512657494 1104 22-23 0.5888604998262892 1104 24-25 0.7312612239680689 1104 26-27 0.8173731947588365 1104 28-29 0.8775444284506051 1104 30-31 0.7093283332700082 1104 32-33 0.5436351828685915 1104 34-35 0.6590757524716295 1104 36-37 0.6806649179129849 1104 38-39 0.680637608929402 1104 40-41 0.7516122903218374 1104 42-43 0.9042873297000114 1104 44-45 0.657951009037788 1104 46-47 0.6397344810218897 1104 48-49 0.5377610359120126 1104 50-51 0.4849571635077652 1104 52-53 0.6113145086587295 1104 54-55 0.5713496243172287 1104 56-57 0.7153698940424249 1104 58-59 0.6959819562383132 1104 60-61 0.624209070906474 1104 62-63 0.55596727235449 1104 64-65 0.4017877839950046 1104 66-67 -2.9190404999398893 1104 68-69 -7.741632142593772 1104 70-71 -9.26004798489317 1104 72-73 -8.530097988491836 1104 74-75 -7.208396054938355 1104 76-77 -3.7700847889715874 1104 78-79 -0.7418177500423901 1104 80-81 0.11702250613805631 1104 82-83 0.12345310780504448 1104 84-85 0.19147903481650275 1104 86-87 0.3379378320080839 1104 88-89 0.3501895316194634 1104 90-91 0.14343090585968454 1104 92-93 0.4402106316424401 1104 94-95 1.391929619284575 1104 96-97 1.506105381227016 1104 98-99 1.2800768251619914 1104 100-101 1.2595696375088963 1105 1 0.4730746053780095 1105 2 0.40896292174165083 1105 3 0.5131333933331703 1105 4 0.33520507335008887 1105 5 0.4894375091051373 1105 6 0.5063058593266732 1105 7 0.5583708146796056 1105 8 0.5453140811300656 1105 9 0.7192672550698731 1105 10-11 0.812441260806466 1105 12-13 0.814885688563642 1105 14-15 0.9113765075704876 1105 16-17 0.9518844436336309 1105 18-19 0.952338484671472 1105 20-21 0.9651915802378781 1105 22-23 1.0872281255097889 1105 24-25 1.056722116590059 1105 26-27 1.1091670026857372 1105 28-29 1.044724695455642 1105 30-31 0.9850733647643111 1105 32-33 1.1056890530475627 1105 34-35 1.3086981841490584 1105 36-37 1.0780629329000035 1105 38-39 0.9573580071562731 1105 40-41 1.0289639766231957 1105 42-43 1.1992975079120214 1105 44-45 1.116097123101298 1105 46-47 1.0290587914971425 1105 48-49 1.0071050097174066 1105 50-51 1.0575348053259361 1105 52-53 1.304442354465678 1105 54-55 1.3202373666063707 1105 56-57 1.3243306698652617 1105 58-59 1.4699879835880694 1105 60-61 1.4794218082738553 1105 62-63 1.6124926821437775 1105 64-65 1.636320238856527 1105 66-67 2.2274196168513676 1105 68-69 -5.196409149016642 1105 70-71 -5.2295398582193044 1105 72-73 -1.384716560761877 1105 74-75 1.3755452658774274 1105 76-77 1.5229954880842982 1105 78-79 1.330095923633607 1105 80-81 1.3430437779989006 1105 82-83 1.457847062367705 1105 84-85 1.3492192051754266 1105 86-87 1.5981907836303257 1105 88-89 1.4791054873712461 1105 90-91 1.542959098490023 1105 92-93 1.5612071012078168 1105 94-95 2.4874036964873376 1105 96-97 2.506029456840203 1105 98-99 2.2912632320704986 1105 100-101 2.1067247635700355 1106 1 0.12317689987299119 1106 2 0.16944386390457922 1106 3 0.1516884870058952 1106 4 0.11233721021048382 1106 5 0.3160516569235057 1106 6 0.27009737366370246 1106 7 0.26613243631366856 1106 8 0.2461285349539466 1106 9 0.44359237089502557 1106 10-11 0.407150491192084 1106 12-13 0.40352729259127074 1106 14-15 0.4514879779382497 1106 16-17 0.3757868898436598 1106 18-19 0.3298992126383524 1106 20-21 0.505758624982505 1106 22-23 0.4860939794395662 1106 24-25 0.4671422927402844 1106 26-27 0.5895596473154825 1106 28-29 0.703683911891126 1106 30-31 0.7287319547899074 1106 32-33 0.7344287138504839 1106 34-35 0.8143134553923801 1106 36-37 0.6996831953654734 1106 38-39 0.7471267236539916 1106 40-41 0.8070000432857327 1106 42-43 0.76498435798689 1106 44-45 0.6783738477576122 1106 46-47 0.6909297818085278 1106 48-49 0.6187813127089044 1106 50-51 0.6663802062099293 1106 52-53 0.8314830837905092 1106 54-55 0.8122703267784388 1106 56-57 0.7238378398450322 1106 58-59 0.8793426327294611 1106 60-61 0.7335891431813053 1106 62-63 0.8341985867388892 1106 64-65 0.8381104705614568 1106 66-67 1.6330714315982426 1106 68-69 -5.274422700354506 1106 70-71 -6.298037611954719 1106 72-73 -2.32180047112357 1106 74-75 0.4756495894831261 1106 76-77 0.8510615719279322 1106 78-79 0.6278111449686143 1106 80-81 0.6261130147684142 1106 82-83 0.761119657003281 1106 84-85 0.7823305459922771 1106 86-87 0.7680332036726334 1106 88-89 0.7758983900882654 1106 90-91 0.7857699006950405 1106 92-93 0.8318914461113742 1106 94-95 1.8055628423722005 1106 96-97 1.8576099097142986 1106 98-99 1.6253003644063675 1106 100-101 1.4518606925261892 1107 1 0.09568818466505746 1107 2 -0.017175156894914778 1107 3 -0.03281942330207954 1107 4 -0.04011808013243012 1107 5 0.06321051020434965 1107 6 0.11801065303396996 1107 7 0.0762100297977426 1107 8 0.035128747120516834 1107 9 0.13670730011928356 1107 10-11 0.16974227182194568 1107 12-13 0.17017387114641025 1107 14-15 0.14373747865640496 1107 16-17 0.0736571450728789 1107 18-19 0.24640243934935313 1107 20-21 0.22757993691542566 1107 22-23 0.2357464205898978 1107 24-25 0.4029750159951462 1107 26-27 0.37816924993731504 1107 28-29 0.25574229274950966 1107 30-31 0.3162602580494962 1107 32-33 0.22924709634459361 1107 34-35 0.3078018137143701 1107 36-37 0.34247903164298066 1107 38-39 0.17085184553027943 1107 40-41 0.34944583791327233 1107 42-43 0.4263816129391458 1107 44-45 0.3159051848132819 1107 46-47 0.21424496945645188 1107 48-49 0.2929494883074071 1107 50-51 0.19508735234648356 1107 52-53 0.41363967599706086 1107 54-55 0.2994111804209396 1107 56-57 0.5320524369051327 1107 58-59 0.3825241355220186 1107 60-61 0.2684383323146591 1107 62-63 0.39616271014176974 1107 64-65 0.41424969086656915 1107 66-67 1.163923743749173 1107 68-69 -5.3905544895188235 1107 70-71 -6.033317294761712 1107 72-73 -2.4202788199100134 1107 74-75 0.203814157453305 1107 76-77 0.14616001296833048 1107 78-79 0.22604094818478515 1107 80-81 0.2449875016452907 1107 82-83 0.28598154957689204 1107 84-85 0.33580010672781313 1107 86-87 0.4570250911558382 1107 88-89 0.41134248207242763 1107 90-91 0.43970232444717894 1107 92-93 0.5158330920725511 1107 94-95 1.243112083471761 1107 96-97 1.3351195561237077 1107 98-99 0.943236518731922 1107 100-101 0.9656867701079932 1108 1 0.00731402699078032 1108 2 -0.027840095707141188 1108 3 0.0367762537196441 1108 4 0.033464871668414276 1108 5 0.10628286563414235 1108 6 0.1249219381595239 1108 7 0.11279000002846118 1108 8 0.0496144484884411 1108 9 0.14482734064455371 1108 10-11 0.19356601979391996 1108 12-13 0.16805284626930472 1108 14-15 0.12552876025940662 1108 16-17 0.016231747655076845 1108 18-19 0.17792456929467448 1108 20-21 0.2470004611152703 1108 22-23 0.1854320765465971 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1.71493275006236 2312 100-101 1.6200037476404994 2313 1 -0.25852108001251395 2313 2 -0.2179304084106164 2313 3 -0.19720947906697361 2313 4 -0.08015864640141501 2313 5 -0.15091092454241561 2313 6 -0.06224579843128453 2313 7 -3.560537831966169 2313 8 -1.2007562229448752 2313 9 -0.7305750344089361 2313 10-11 -0.04462093126600308 2313 12-13 -1.3789462846868048 2313 14-15 -0.4153975004445627 2313 16-17 -0.1167589447987254 2313 18-19 0.19659523116148137 2313 20-21 0.11789318509111979 2313 22-23 -1.1319985315125152 2313 24-25 -2.7067435550015233 2313 26-27 -1.9780810927385701 2313 28-29 -0.33389737653434537 2313 30-31 -0.11006473285550555 2313 32-33 -2.3732099010581607 2313 34-35 -2.6037539999823878 2313 36-37 -1.036350609614594 2313 38-39 -0.5725577543775415 2313 40-41 -0.4321973762681566 2313 42-43 -0.4668007698034131 2313 44-45 -0.1782444311069753 2313 46-47 -0.1887373085963162 2313 48-49 -0.19918420991308494 2313 50-51 -0.12667000353724944 2313 52-53 0.22681237079535066 2313 54-55 0.29591318181205395 2313 56-57 0.5130526825253838 2313 58-59 0.11913055870856226 2313 60-61 0.5481431175985634 2313 62-63 0.3151035017306576 2313 64-65 0.08840384793387202 2313 66-67 1.4661516676975381 2313 68-69 4.651632364954583 2313 70-71 4.594713322617103 2313 72-73 2.6811446383230297 2313 74-75 1.842969314785897 2313 76-77 1.1678604631409826 2313 78-79 0.6282067253761596 2313 80-81 0.6254068517015057 2313 82-83 0.4846218813060048 2313 84-85 0.5979504615576836 2313 86-87 0.6546190986775393 2313 88-89 0.6372446939085386 2313 90-91 0.7702913549012216 2313 92-93 0.9119225342293902 2313 94-95 1.6891468081258942 2313 96-97 1.7066636108639912 2313 98-99 1.4921575639939881 2313 100-101 1.1817264727312917 2314 1 -0.21781844868347378 2314 2 -0.19178290678070198 2314 3 -0.14536449752006675 2314 4 -0.1967102465854822 2314 5 -1.3925407273667645 2314 6 -2.7149748177328306 2314 7 -0.7808321963219385 2314 8 -0.1745308424808627 2314 9 0.02569386726085554 2314 10-11 0.13175657748103475 2314 12-13 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0.11522200485309142 2314 78-79 0.4920381744146596 2314 80-81 0.7739633181828225 2314 82-83 0.5569435948564099 2314 84-85 0.4679190713719912 2314 86-87 0.3104524686170471 2314 88-89 0.43510825096283057 2314 90-91 0.932770302676051 2314 92-93 0.9518884031276222 2314 94-95 0.7543617611486404 2314 96-97 1.1228807013383104 2314 98-99 0.6194929575981902 2314 100-101 0.6179013510035887 2315 1 -0.36519525657832475 2315 2 -0.25866063698303776 2315 3 -0.32806142798992965 2315 4 -0.09434733006088436 2315 5 -1.1769048154851376 2315 6 -0.36052615997643045 2315 7 -0.1775699515005158 2315 8 -0.10689576502684162 2315 9 0.13946274392545632 2315 10-11 -1.7259380119404852 2315 12-13 -1.2509261987619453 2315 14-15 -0.01651173901755243 2315 16-17 -1.620041314449999 2315 18-19 -3.003607462537822 2315 20-21 -2.059376163452562 2315 22-23 -1.586032667383698 2315 24-25 -0.9485613721063046 2315 26-27 -0.2654661145552524 2315 28-29 -0.5162118072320467 2315 30-31 -2.895689380439279 2315 32-33 -2.417984525829766 2315 34-35 -1.421393115657473 2315 36-37 -4.655081871889344 2315 38-39 -2.0147367375219787 2315 40-41 -1.5270559012788212 2315 42-43 -1.486832322104675 2315 44-45 -1.3477880426139777 2315 46-47 -1.1438973369773038 2315 48-49 -4.490331164933938 2315 50-51 -3.7444390521900317 2315 52-53 -2.2080705245987566 2315 54-55 -3.834806514569536 2315 56-57 -5.9173843201519105 2315 58-59 -6.705062571404802 2315 60-61 -5.335022331078942 2315 62-63 -6.28017248238632 2315 64-65 -5.721108875964038 2315 66-67 -3.7929785744929667 2315 68-69 -1.7423821880626882 2315 70-71 0.6887793923951904 2315 72-73 -0.9328307701867367 2315 74-75 -2.57137044604589 2315 76-77 -4.736476740558924 2315 78-79 -3.813302327874595 2315 80-81 -3.5612978520680763 2315 82-83 -6.829535020589983 2315 84-85 -4.702161419976964 2315 86-87 -5.192344851250066 2315 88-89 -3.602171003015279 2315 90-91 -3.369590150313524 2315 92-93 -4.364128176670107 2315 94-95 -2.2896376644405585 2315 96-97 -1.2765472064835244 2315 98-99 -2.613779932868834 2315 100-101 -1.8728103973879229 2316 1 -0.3830405593327235 2316 2 -0.396625114388403 2316 3 -1.0034019147644102 2316 4 -0.3677945015102466 2316 5 -0.3605313039042102 2316 6 -2.341249659386527 2316 7 -0.4737429425025397 2316 8 -0.47022907208359044 2316 9 -0.0570074192943224 2316 10-11 -0.06600360465936461 2316 12-13 -0.7450767927201909 2316 14-15 -1.043594932507709 2316 16-17 -1.4778878721102586 2316 18-19 -0.3111301453991473 2316 20-21 -4.061806428787918 2316 22-23 -4.029592082040189 2316 24-25 -2.700072056602501 2316 26-27 -2.1625066229322627 2316 28-29 -1.4836479557348738 2316 30-31 -0.37378052371026627 2316 32-33 -2.0149109142146884 2316 34-35 -2.555623770379917 2316 36-37 -4.696593257890662 2316 38-39 -2.15621654384924 2316 40-41 -1.805184006941893 2316 42-43 -3.164230225440459 2316 44-45 -0.8758978143891198 2316 46-47 -0.8840255236996484 2316 48-49 -0.5270422971512474 2316 50-51 -0.22549240233082202 2316 52-53 -2.876134627674304 2316 54-55 -1.0824838040620612 2316 56-57 0.005526239697744018 2316 58-59 0.083583014913728 2316 60-61 -1.2443247666553319 2316 62-63 -0.9484724376922422 2316 64-65 0.09106942004102336 2316 66-67 1.252249634038943 2316 68-69 3.734203346869993 2316 70-71 4.019537899378911 2316 72-73 2.199919836145394 2316 74-75 1.1809038916628154 2316 76-77 0.0731968796079876 2316 78-79 0.2558343852801208 2316 80-81 -0.2083744111194008 2316 82-83 -0.11173922132364211 2316 84-85 0.35023217360123127 2316 86-87 -0.1577758974776593 2316 88-89 0.2894565692729927 2316 90-91 0.3698136786303863 2316 92-93 0.5610316984157997 2316 94-95 1.2364892207150895 2316 96-97 1.189412755490281 2316 98-99 0.20487693406600016 2316 100-101 -0.12465363989903722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 52.0 4 142.0 5 300.0 6 802.0 7 2013.0 8 4418.0 9 7389.0 10 11701.0 11 17456.0 12 23328.0 13 29605.0 14 36243.0 15 45064.0 16 53761.0 17 61340.0 18 57124.0 19 49460.0 20 48880.0 21 49726.0 22 52962.0 23 60669.0 24 73922.0 25 92615.0 26 116354.0 27 145997.0 28 178323.0 29 214624.0 30 252800.0 31 295974.0 32 343638.0 33 399363.0 34 464497.0 35 538270.0 36 582423.0 37 505453.0 38 218231.0 39 13565.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.267504981327825 20.419669810825443 15.351365637684259 22.961459570162475 2 15.783117205767153 21.221670527997922 39.088024484287416 23.907187781947513 3 18.998964244959534 25.065311595088513 30.544750896852147 25.390973263099802 4 13.103757142480793 17.730921972526048 36.934244311815156 32.231076573178 5 13.452646991593124 38.07040368142053 34.08059366709148 14.39635565989487 6 31.531045613431814 36.88129779769836 17.242102640175055 14.34555394869477 7 28.113767064257416 33.33009804315785 20.558677830662667 17.997457061922066 8 26.361401852553566 35.23880700193385 19.389338319113573 19.010452826399018 9 26.223301180491358 16.74650900635457 20.47094864584289 36.559241167311185 10-11 25.001163714068227 27.027521689154245 27.059620389538182 20.91169420723935 12-13 25.838384182521075 24.411126017655572 27.695493564314567 22.05499623550879 14-15 22.821888758442867 25.67573161960673 26.355617946035757 25.146761675914647 16-17 21.91473879881933 28.95252813498414 26.925301890699853 22.207431175496676 18-19 21.706497378342586 27.296771658744895 29.572885899047595 21.423845063864924 20-21 22.801756287349654 26.287798303275007 29.5689176794529 21.34152772992244 22-23 22.294711429613322 26.43453261578559 29.369746326179445 21.90100962842164 24-25 22.060076961610907 26.5982845170963 29.567231079544364 21.774407441748437 26-27 22.077667918267483 26.751570971629413 29.428283986349918 21.742477123753186 28-29 21.769963838766763 26.941639305938097 29.562979535050047 21.725417320245093 30-31 22.024027656228892 26.642405530928837 29.444605335772717 21.88896147706955 32-33 21.938984925706173 26.655743667275217 29.36285078794564 22.042420619072974 34-35 22.420431084382887 26.74077369832968 29.190192420227046 21.648602797060388 36-37 22.27386015525087 26.564782044037315 29.228355589923567 21.93300221078825 38-39 22.058378853825435 26.831630986889028 29.300543906182675 21.80944625310286 40-41 22.298009469274437 26.610654173173693 29.253989737487917 21.837346620063954 42-43 22.147183982406947 26.692333445923737 29.03850998378163 22.121972587887683 44-45 22.186060925456903 26.91581182193851 28.799692125039112 22.098435127565477 46-47 22.271018469289743 27.021040502449225 28.44120526983606 22.266735758424968 48-49 22.3922617048911 27.053795561815836 28.313968065226952 22.23997466806611 50-51 22.268247012315562 27.252397027642104 28.195921196588586 22.283434763453748 52-53 22.572837473952635 27.2907112001294 27.90752233650818 22.228928989409784 54-55 22.259057851803167 27.116769166001355 28.134798847863607 22.48937413433187 56-57 22.37247846982486 27.41258635947338 27.925878575003793 22.289056595697968 58-59 22.45433786222813 27.12556482413424 27.992108088050806 22.427989225586824 60-61 22.49732222962443 27.193706551231834 28.135774596214812 22.173196622928927 62-63 22.30363192154666 27.11074712354523 28.194288014306306 22.3913329406018 64-65 22.218133913386772 27.118742943746778 28.073634326845205 22.589488816021248 66-67 21.73752339945258 27.201427957032013 28.487106373352095 22.573942270163304 68-69 21.703470176390535 26.885157997264614 28.94415221367057 22.467219612674278 70-71 21.246907964870704 26.89320561962431 29.286194306029984 22.573692109475 72-73 22.200505032248735 26.964338701303653 28.152538885377858 22.682617381069754 74-75 21.95096672479922 27.23559077009501 28.00818113871041 22.805261366395364 76-77 22.138574624328715 27.253829616440072 27.768067207579435 22.83952855165178 78-79 22.07664629189124 27.257802582847475 27.753130304005175 22.912420821256116 80-81 22.02470277829499 27.33830584618554 27.684463653974905 22.952527721544563 82-83 22.159126360728383 27.370495460920896 27.552252742453142 22.91812543589758 84-85 22.09300759375425 27.261740191276957 27.43679249898633 23.20845971598246 86-87 22.00038843416957 27.19299661709691 27.699268363146135 23.107346585587386 88-89 22.04025466786096 27.186806347601582 27.45760190537388 23.315337079163577 90-91 21.91490992747886 27.27354302549303 27.609119943046412 23.202427103981698 92-93 22.044073687373587 27.094149859886553 27.637122903473156 23.224653549266712 94-95 21.54244016060526 27.034974143918657 27.885780256954916 23.536805438521164 96-97 21.409183077828757 26.913266939297962 28.042707317918065 23.63484266495522 98-99 21.614909047145716 26.820901946042913 27.558172533322633 24.006016473488735 100-101 21.55224959762288 26.777783830630185 27.688844868144113 23.981121703602824 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1719.0 1 1178.5 2 698.5 3 838.5 4 893.0 5 928.5 6 1370.5 7 2633.0 8 4188.0 9 5272.0 10 5383.0 11 5015.0 12 5007.0 13 5360.5 14 5787.5 15 6145.0 16 6647.0 17 7163.0 18 7744.0 19 8469.0 20 9525.5 21 10870.0 22 12654.5 23 15002.5 24 17792.0 25 22068.0 26 28719.5 27 36533.5 28 43508.5 29 51157.5 30 60867.0 31 71299.5 32 81908.0 33 92386.5 34 103279.5 35 113008.0 36 123263.5 37 133934.5 38 140594.5 39 144401.0 40 148766.5 41 155804.0 42 162963.5 43 169063.5 44 174424.5 45 179226.5 46 184192.0 47 189963.5 48 198977.0 49 201324.5 50 193609.0 51 186214.5 52 179909.0 53 174776.5 54 168084.5 55 156799.5 56 143289.0 57 127704.5 58 112425.5 59 97838.0 60 82633.0 61 66467.0 62 51426.5 63 39068.0 64 29056.5 65 22036.0 66 16535.0 67 12164.5 68 8486.5 69 5730.0 70 4121.5 71 3119.0 72 2405.5 73 1805.0 74 1331.0 75 964.5 76 613.5 77 322.0 78 194.0 79 117.5 80 62.0 81 44.0 82 32.0 83 17.5 84 11.0 85 8.0 86 4.5 87 2.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022620620696362538 2 0.019986170124894745 3 0.0 4 0.0 5 9.903949516796208E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.268303340542748E-4 18-19 0.0012379936895995259 20-21 9.903949516796207E-6 22-23 0.0 24-25 0.0 26-27 4.555816777726255E-4 28-29 0.01898587122369833 30-31 0.004456777282558293 32-33 0.008457972887343962 34-35 0.00944836783902358 36-37 0.09985161902833935 38-39 0.10216914321526968 40-41 0.1161931357310531 42-43 0.10174327338604743 44-45 0.09676158677909895 46-47 0.0981778515600008 48-49 0.10011902566529286 50-51 0.09810852391338323 52-53 0.07596329279382691 54-55 0.06447471135434331 56-57 0.03670403690924674 58-59 0.015677952085088393 60-61 0.008873938767049401 62-63 0.0017629030139897249 64-65 0.004367641736907127 66-67 0.40266487550438335 68-69 0.778886205798921 70-71 0.8615346645165852 72-73 0.26869415039068106 74-75 0.14140859120081622 76-77 0.014400342597421687 78-79 0.008299509695075221 80-81 0.0014063608313850614 82-83 0.0 84-85 0.0 86-87 3.268303340542748E-4 88-89 6.536606681085496E-4 90-91 0.0033673428357107104 92-93 0.0016242477207545779 94-95 0.2138856937647309 96-97 0.21868910928037705 98-99 0.013538698989460415 100-101 0.007249691046294823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5048491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.07241794543451 #Duplication Level Percentage of deduplicated Percentage of total 1 63.81662457307715 24.296532026130794 2 15.049170920138554 11.459166500075886 3 6.859926764201046 7.8352199652520325 4 4.013545603543495 6.112215426446765 5 2.4740620146035748 4.7096761521455495 6 1.682779525921406 3.8440491252539957 7 1.191861195475772 3.1763926296969167 8 0.8808861605715699 2.6829972854103956 9 0.6709309886887873 2.2989568512512792 >10 3.1157290110409774 20.175098171487857 >50 0.15292684904151232 4.013812608649299 >100 0.08462472220901832 6.137111664213925 >500 0.00535629280771223 1.420271377073551 >1k 0.0014178408115411005 0.872666825813 >5k 0.0 0.0 >10k+ 1.5753786794830434E-4 0.9658333910989657 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19818 0.3925529430477344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17165 0.3400025869116138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11326 0.22434426445446767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9807899033592413E-5 2 1.9807899033592413E-5 0.0 0.0 0.0 1.9807899033592413E-5 3 1.9807899033592413E-5 0.0 0.0 0.0 1.9807899033592413E-5 4 1.9807899033592413E-5 0.0 0.0 0.0 1.9807899033592413E-5 5 1.9807899033592413E-5 0.0 0.0 0.0 1.9807899033592413E-5 6 9.903949516796207E-5 0.0 0.0 0.0 1.9807899033592413E-5 7 1.584631922687393E-4 0.0 0.0 0.0 1.9807899033592413E-5 8 1.584631922687393E-4 0.0 0.0 0.0 3.9615798067184826E-5 9 1.584631922687393E-4 0.0 0.0 0.0 3.9615798067184826E-5 10-11 1.584631922687393E-4 0.0 0.0 0.0 3.9615798067184826E-5 12-13 1.584631922687393E-4 0.0 0.0 2.971184855038862E-5 3.9615798067184826E-5 14-15 1.7827109130233172E-4 0.0 0.0 3.9615798067184826E-5 1.0894344468475827E-4 16-17 1.9807899033592413E-4 0.0 0.0 3.9615798067184826E-5 1.1884739420155448E-4 18-19 2.1788688936951654E-4 0.0 0.0 7.923159613436965E-5 1.1884739420155448E-4 20-21 2.3769478840310896E-4 0.0 0.0 1.485592427519431E-4 1.1884739420155448E-4 22-23 2.3769478840310896E-4 0.0 0.0 3.169263845374786E-4 1.485592427519431E-4 24-25 3.5654218260466343E-4 0.0 0.0 5.150053748734027E-4 2.1788688936951654E-4 26-27 3.5654218260466343E-4 0.0 0.0 0.0010201068002300092 2.3769478840310896E-4 28-29 4.258698292222369E-4 0.0 0.0 0.0022581004898295353 2.3769478840310896E-4 30-31 4.555816777726255E-4 0.0 0.0 0.006100832902346464 2.3769478840310896E-4 32-33 4.555816777726255E-4 0.0 0.0 0.014301303102253722 3.169263845374786E-4 34-35 4.852935263230141E-4 0.0 0.0 0.027216053272155975 4.159658797054407E-4 36-37 5.150053748734027E-4 0.0 0.0 0.04917310935089317 4.258698292222369E-4 38-39 5.645251224573838E-4 0.0 0.0 0.08328231148673931 4.357737787390331E-4 40-41 5.942369710077724E-4 0.0 0.0 0.12165021191480782 4.456777282558293E-4 42-43 6.536606681085496E-4 0.0 0.0 0.16151360871991255 4.753895768062179E-4 44-45 7.527001632765117E-4 0.0 0.0 0.20976565076574366 5.051014253566065E-4 46-47 8.913554565116586E-4 0.0 0.0 0.2705759007988724 5.348132739069952E-4 48-49 9.309712545788434E-4 0.0 0.0 0.3350704200522493 5.348132739069952E-4 50-51 9.309712545788434E-4 0.0 0.0 0.4065472237149675 5.447172234237913E-4 52-53 9.408752040956396E-4 0.0 0.0 0.4777863325892826 5.843330214909761E-4 54-55 9.903949516796207E-4 0.0 0.0 0.5584837132521381 6.338527690749572E-4 56-57 0.0010102028507132132 0.0 0.0 0.6489860039366219 6.338527690749572E-4 58-59 0.0010102028507132132 0.0 0.0 0.7384780917703924 6.338527690749572E-4 60-61 0.0010102028507132132 0.0 0.0 0.8306343420241811 6.338527690749572E-4 62-63 0.0010696265478139903 1.9807899033592413E-5 0.0 0.9238404109267502 6.536606681085496E-4 64-65 0.0010696265478139903 1.9807899033592413E-5 0.0 1.0260491699400869 6.536606681085496E-4 66-67 0.0010696265478139903 1.9807899033592413E-5 0.0 1.1327048022864654 6.536606681085496E-4 68-69 0.0010894344468475828 1.9807899033592413E-5 0.0 1.2596932429908265 6.536606681085496E-4 70-71 0.0011785699924987485 1.9807899033592413E-5 0.0 1.426406425207057 6.73468567142142E-4 72-73 0.0012379936895995259 1.9807899033592413E-5 0.0 1.611273546887575 7.725080623101041E-4 74-75 0.0012677055381499144 1.9807899033592413E-5 0.0 1.8127892077058272 7.725080623101041E-4 76-77 0.0012677055381499144 1.9807899033592413E-5 0.0 2.0364798114921867 7.725080623101041E-4 78-79 0.0012776094876667107 1.9807899033592413E-5 0.0 2.2920116129750454 7.923159613436965E-4 80-81 0.0014954963770362271 1.9807899033592413E-5 0.0 2.5726202146344326 8.022199108604928E-4 82-83 0.001584631922687393 1.9807899033592413E-5 0.0 2.8645094147934502 8.517396584444738E-4 84-85 0.0015945358722041893 1.9807899033592413E-5 0.0 3.156190632012615 8.517396584444738E-4 86-87 0.0016143437712377818 1.9807899033592413E-5 0.0 3.460410249320044 8.616436079612699E-4 88-89 0.0016242477207545779 3.9615798067184826E-5 0.0 3.798461758176849 8.715475574780662E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17155 0.0 36.415733 1 GTATCAA 26395 0.0 31.802502 1 ATCAACG 32790 0.0 25.37346 3 TCAACGC 32935 0.0 25.276138 4 TATCAAC 33050 0.0 25.249784 2 CAACGCA 33535 0.0 24.767387 5 AACGCAG 34320 0.0 24.228497 6 GTACATG 23600 0.0 23.598818 1 TACATGG 23915 0.0 22.629005 2 ACGCAGA 38775 0.0 21.395922 7 CGCAGAG 38965 0.0 21.218632 8 ACATGGG 24495 0.0 21.025616 3 GAGTACT 19405 0.0 21.010378 12-13 GTACTTT 20065 0.0 20.248442 14-15 ACCGTCG 445 0.0 20.229736 8 GCAGAGT 40525 0.0 19.84063 9 AGTACTT 20030 0.0 19.586012 12-13 AGAGTAC 33345 0.0 19.580109 10-11 CATGGGA 15035 0.0 18.277685 4 ACTTTTT 23995 0.0 17.38622 16-17 >>END_MODULE