FastQCFastQC Report
Fri 27 May 2016
SRR939486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015320
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135480.27013231458810205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99510.19841206543151782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.13672108659068613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA227450.036.6944241
GGTATCA142750.036.3251531
TATCAAC283950.028.9408972
ATCAACG289100.028.5581043
TCAACGC291600.028.4226064
CAACGCA292950.027.769235
AACGCAG297950.027.1496016
GTACATG201400.025.446011
TACATGG203450.024.326772
ACGCAGA330050.023.9652587
CGCAGAG333750.023.4815888
ACATGGG215700.022.6085473
GAGTACT161350.022.30878412-13
GCAGAGT334450.021.878549
GTACTTT176900.020.70605514-15
AGAGTAC275400.020.4433510-11
AGTACTT171100.020.01097112-13
CATGGGA133150.019.4115314
GTATAGG13400.019.2166861
GTGTAGC33900.018.468651
Produced by FastQC (version 0.11.5)