##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939486_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5015320 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.911199883556783 31.0 26.0 31.0 10.0 33.0 2 27.02369818874967 31.0 26.0 31.0 16.0 34.0 3 27.35674872191605 31.0 26.0 31.0 16.0 34.0 4 31.209268202228373 35.0 30.0 37.0 19.0 37.0 5 31.669032484467593 35.0 32.0 37.0 19.0 37.0 6 31.485142523308582 35.0 30.0 37.0 17.0 37.0 7 31.46789018447477 35.0 30.0 37.0 17.0 37.0 8 31.39855004266926 35.0 30.0 37.0 17.0 37.0 9 32.48999645087452 35.0 31.0 39.0 17.0 39.0 10-11 31.544816781381847 35.0 29.5 38.5 14.0 39.0 12-13 31.29258202866417 35.0 29.0 38.0 15.5 39.0 14-15 32.14822792962363 36.0 30.0 39.0 17.0 40.0 16-17 32.08426102422179 36.0 29.5 39.0 17.0 40.0 18-19 32.02061144652784 36.0 29.5 39.0 16.5 40.0 20-21 31.8919715990206 36.0 29.0 39.0 16.0 40.0 22-23 32.10217423813435 36.0 29.5 39.0 16.5 40.0 24-25 32.18261925460389 36.0 30.0 39.0 16.0 41.0 26-27 32.31082563026885 36.0 30.0 39.0 16.0 41.0 28-29 31.896142718709875 36.0 30.0 39.0 13.0 41.0 30-31 31.619425480328275 36.0 29.0 39.0 12.0 40.0 32-33 31.513485779571393 36.0 29.5 39.0 10.0 40.0 34-35 31.070550333777305 35.0 28.0 39.0 9.0 40.0 36-37 30.727016621073034 35.0 27.0 39.0 9.0 40.0 38-39 30.36328389813611 35.0 26.0 38.0 9.0 40.0 40-41 30.237897681503874 35.0 26.5 38.0 8.5 40.0 42-43 29.996183294386 35.0 26.0 38.0 8.0 40.0 44-45 29.66479626424635 34.5 25.5 38.0 6.5 40.0 46-47 29.104301918920427 34.0 24.0 38.0 2.0 40.0 48-49 28.788715475782205 34.0 24.0 38.0 2.0 40.0 50-51 28.1268803386424 33.0 23.0 37.5 2.0 39.5 52-53 28.234479155866424 33.5 23.5 38.0 2.0 39.5 54-55 28.37603502867215 34.0 23.5 38.0 2.0 40.0 56-57 27.934089250536356 33.5 23.0 38.0 2.0 40.0 58-59 27.562668184682135 33.0 22.0 38.0 2.0 40.0 60-61 26.965474386479826 32.5 20.5 37.5 2.0 40.0 62-63 26.409426218067843 32.0 18.5 37.0 2.0 39.0 64-65 25.90040934576458 31.5 17.5 36.5 2.0 39.0 66-67 25.534590315274002 31.0 15.0 36.0 2.0 39.0 68-69 24.83844041855754 31.0 11.5 35.5 2.0 38.5 70-71 24.22185224472217 30.0 8.0 35.0 2.0 38.0 72-73 23.743860112614946 30.0 7.0 35.0 2.0 37.5 74-75 23.012930580700733 29.0 4.0 34.0 2.0 37.0 76-77 22.24452826539483 29.0 2.0 34.0 2.0 36.0 78-79 21.75166928530981 28.5 2.0 34.0 2.0 36.0 80-81 21.187940849237936 27.5 2.0 34.0 2.0 35.0 82-83 20.434966163674503 26.0 2.0 33.0 2.0 35.0 84-85 19.740102725249834 26.0 2.0 33.0 2.0 35.0 86-87 18.95503756490114 25.0 2.0 32.0 2.0 34.5 88-89 18.160167546637105 23.5 2.0 32.0 2.0 34.0 90-91 17.3548244179833 20.5 2.0 31.5 2.0 34.0 92-93 16.508713601524928 18.5 2.0 31.0 2.0 34.0 94-95 15.42337119067178 4.5 2.0 31.0 2.0 34.0 96-97 14.30018174313902 2.0 2.0 30.5 2.0 34.0 98-99 13.249708393482369 2.0 2.0 29.5 2.0 34.0 100-101 11.864909616933716 2.0 2.0 27.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.943099285994929 1101 2 3.7408164852291854 1101 3 3.465506560931196 1101 4 3.242682531057042 1101 5 2.469005174586453 1101 6 2.7306505436308868 1101 7 2.5227342010833453 1101 8 2.598639804272196 1101 9 2.666229184151838 1101 10-11 3.550647030484594 1101 12-13 3.6681743333106063 1101 14-15 2.983571812549325 1101 16-17 2.9669772893394253 1101 18-19 3.043168503974968 1101 20-21 2.865361934008824 1101 22-23 2.8367205432584797 1101 24-25 3.177635741650107 1101 26-27 2.957609692265258 1101 28-29 3.1876422961830464 1101 30-31 3.2355637814903666 1101 32-33 3.1006657086735707 1101 34-35 3.3820972759599215 1101 36-37 3.3351482700981485 1101 38-39 3.4460730329881777 1101 40-41 2.9924174470015075 1101 42-43 3.050645013359169 1101 44-45 3.233287275276009 1101 46-47 3.2590926475613173 1101 48-49 3.1207332335729987 1101 50-51 3.1063755896677954 1101 52-53 3.047265723816306 1101 54-55 2.9299980032643766 1101 56-57 3.244425316017491 1101 58-59 3.0784791701337895 1101 60-61 3.0095925767109186 1101 62-63 3.055410507372894 1101 64-65 2.9011835206440857 1101 66-67 2.659637283509337 1101 68-69 2.9329388601214426 1101 70-71 2.717715832694058 1101 72-73 2.7457875395040787 1101 74-75 2.628615119146602 1101 76-77 2.626420518130818 1101 78-79 2.3345310837609503 1101 80-81 2.437341590722891 1101 82-83 2.0485928436125 1101 84-85 1.9599348388498932 1101 86-87 2.0364871256523536 1101 88-89 2.0361758453674845 1101 90-91 1.9393779212905926 1101 92-93 1.638331847056456 1101 94-95 1.7455228050197622 1101 96-97 1.6696151168222944 1101 98-99 1.4928593221340822 1101 100-101 1.3237250610077211 1102 1 3.547857978770388 1102 2 2.525412778989132 1102 3 2.741931928954962 1102 4 2.828271549299899 1102 5 1.7967968260207066 1102 6 2.3856015510299784 1102 7 2.3492082270239365 1102 8 2.4261292102336895 1102 9 2.611876488915165 1102 10-11 1.9260163414987979 1102 12-13 3.0416004170434157 1102 14-15 3.0486714711715557 1102 16-17 2.850734813295084 1102 18-19 2.911696872312824 1102 20-21 2.776241741230791 1102 22-23 2.648307821144101 1102 24-25 2.833477523611151 1102 26-27 2.558832061519631 1102 28-29 2.731790216250186 1102 30-31 2.8077541086054474 1102 32-33 2.528355410186858 1102 34-35 2.704189514893457 1102 36-37 2.7068494435520343 1102 38-39 2.6985345057011294 1102 40-41 2.507233653949548 1102 42-43 2.6669745072653477 1102 44-45 2.357942679394167 1102 46-47 2.4737852788475863 1102 48-49 2.3802694129224804 1102 50-51 2.5101595794110168 1102 52-53 2.6086601721797003 1102 54-55 2.4148702826689465 1102 56-57 2.5558303198019985 1102 58-59 2.5704549904959677 1102 60-61 2.68567811166481 1102 62-63 2.6094480846720423 1102 64-65 2.565346063831207 1102 66-67 2.4079440394937457 1102 68-69 2.3730546093246367 1102 70-71 2.44893052995943 1102 72-73 2.2973113818833255 1102 74-75 2.3692931376108675 1102 76-77 2.135568721392186 1102 78-79 2.0277390564507307 1102 80-81 2.034012256435279 1102 82-83 2.0821570231473423 1102 84-85 2.0448719954768926 1102 86-87 1.6826817676176198 1102 88-89 1.6673457464554673 1102 90-91 1.712513602218234 1102 92-93 1.3931829641289504 1102 94-95 1.4458971362952173 1102 96-97 1.3594556527287462 1102 98-99 1.120624713740206 1102 100-101 0.650377241860701 1103 1 3.71834270153542 1103 2 3.68783148245344 1103 3 3.2634353010701247 1103 4 2.9742094783301525 1103 5 2.393458782749402 1103 6 2.4935843948303926 1103 7 2.447936543772272 1103 8 2.3878223828278635 1103 9 2.7877525874831477 1103 10-11 3.398778369377613 1103 12-13 2.609690587302655 1103 14-15 3.202439922604082 1103 16-17 2.6297876147060606 1103 18-19 2.610408654172545 1103 20-21 2.747492580501657 1103 22-23 2.9773781595581568 1103 24-25 2.9495098518236773 1103 26-27 2.6767323310884237 1103 28-29 2.3133267583540267 1103 30-31 2.8516652611372884 1103 32-33 2.867363717715744 1103 34-35 2.6664699265620797 1103 36-37 2.9266797855737003 1103 38-39 3.1340494422171723 1103 40-41 3.082793084347685 1103 42-43 2.8148755785257045 1103 44-45 2.88222040539603 1103 46-47 2.760512272804835 1103 48-49 2.548780972312791 1103 50-51 2.7905156306791916 1103 52-53 2.822428222766437 1103 54-55 2.838887458910822 1103 56-57 2.9518288767571725 1103 58-59 2.9582878177614944 1103 60-61 2.915923429284991 1103 62-63 2.9297875008401597 1103 64-65 3.03240155090894 1103 66-67 2.6984224489376736 1103 68-69 2.8614290794566166 1103 70-71 2.6651497525190315 1103 72-73 2.5003818605934036 1103 74-75 2.594419153504404 1103 76-77 2.644955026495957 1103 78-79 2.3255073475449386 1103 80-81 2.2726998873364828 1103 82-83 2.340368850102216 1103 84-85 2.328536026582526 1103 86-87 2.26021586456217 1103 88-89 2.132400010953557 1103 90-91 1.9041704982689005 1103 92-93 1.9018367235613525 1103 94-95 2.055521944640825 1103 96-97 1.9591485046462918 1103 98-99 1.9446950020485367 1103 100-101 1.8532923928292444 1104 1 3.742726770365497 1104 2 3.511428057845965 1104 3 2.8048066325542393 1104 4 2.8100462965482365 1104 5 1.724346293552717 1104 6 2.2193161535791504 1104 7 2.402118304901279 1104 8 2.2899537443768594 1104 9 2.3536766166602945 1104 10-11 3.2518000443885633 1104 12-13 3.237347330009886 1104 14-15 2.9672781897564526 1104 16-17 3.068030964593845 1104 18-19 2.7691918712089603 1104 20-21 2.6030482478059582 1104 22-23 2.32617707282391 1104 24-25 2.443553799924878 1104 26-27 2.4748195836180216 1104 28-29 2.4516370569176296 1104 30-31 2.0176397783994524 1104 32-33 2.163681741693896 1104 34-35 2.7824846398494287 1104 36-37 3.058864799932298 1104 38-39 3.111439965878521 1104 40-41 2.9976785541718414 1104 42-43 2.9435376576827075 1104 44-45 2.9465778288542843 1104 46-47 2.9496404381160986 1104 48-49 2.7574626097359314 1104 50-51 2.6653799397601396 1104 52-53 2.9395057337105506 1104 54-55 2.774379711034772 1104 56-57 2.8660546490136696 1104 58-59 2.849239018647083 1104 60-61 3.0281151955030303 1104 62-63 3.1047014598904994 1104 64-65 3.1847827044376444 1104 66-67 3.0872243861245927 1104 68-69 3.1772989328400207 1104 70-71 3.1848210972118487 1104 72-73 2.92431304526648 1104 74-75 3.0044799269443168 1104 76-77 3.027720653832205 1104 78-79 2.9275598256516844 1104 80-81 2.9562008102694044 1104 82-83 3.0758784679818376 1104 84-85 2.808988253656878 1104 86-87 2.901956704461302 1104 88-89 2.954038681206015 1104 90-91 2.9597578128190563 1104 92-93 2.823749357048026 1104 94-95 2.812110740308908 1104 96-97 2.814769209722792 1104 98-99 2.6883855000300194 1104 100-101 2.3956226962069866 1105 1 3.431151446703918 1105 2 3.171617275886888 1105 3 2.755436805058544 1105 4 2.6733182635720283 1105 5 -0.2515174506768716 1105 6 1.7066540782453785 1105 7 1.9799880238196224 1105 8 2.0278272846962295 1105 9 2.430119505300368 1105 10-11 2.4841550838448576 1105 12-13 3.13403663380409 1105 14-15 2.9860683641910093 1105 16-17 2.800342214446303 1105 18-19 2.8214335859094177 1105 20-21 2.776859097585117 1105 22-23 2.4891280356409027 1105 24-25 2.165443939210377 1105 26-27 2.4612885308220385 1105 28-29 2.2707233247195937 1105 30-31 2.4873745431713523 1105 32-33 2.481888598174205 1105 34-35 2.790429599936658 1105 36-37 2.6823455484834575 1105 38-39 3.0438086997753224 1105 40-41 2.86709991189084 1105 42-43 2.8616185905219105 1105 44-45 2.6882813129155885 1105 46-47 2.8143263235171077 1105 48-49 2.7827352758282977 1105 50-51 2.8735748885983625 1105 52-53 2.8940148890961517 1105 54-55 2.6819929396034645 1105 56-57 2.971590887437788 1105 58-59 3.030143979882034 1105 60-61 2.936605944256346 1105 62-63 2.9152376363635817 1105 64-65 2.8369261452245134 1105 66-67 2.63778435831836 1105 68-69 2.86279143382961 1105 70-71 2.7910525567327156 1105 72-73 2.790139710168088 1105 74-75 2.7170627701579733 1105 76-77 2.731198259183209 1105 78-79 2.731970446277206 1105 80-81 2.773940229846847 1105 82-83 2.775796562185242 1105 84-85 2.712231912315776 1105 86-87 2.623177087788253 1105 88-89 2.6246898975005735 1105 90-91 2.5385286911348253 1105 92-93 2.5114744105052544 1105 94-95 2.5529157712550816 1105 96-97 2.597648615827511 1105 98-99 2.491549763539748 1105 100-101 2.267195841800408 1106 1 3.0879594459362814 1106 2 2.606642954264224 1106 3 2.1263834517118383 1106 4 1.3048497609053342 1106 5 1.6941943710248744 1106 6 2.178825752999643 1106 7 2.032598513862496 1106 8 0.4720363242442538 1106 9 1.9082586809983582 1106 10-11 0.9035249526507805 1106 12-13 1.5541047430572696 1106 14-15 1.7240262043075951 1106 16-17 1.8492227692637542 1106 18-19 2.1004294456302404 1106 20-21 2.0455008653730857 1106 22-23 2.476269471542224 1106 24-25 2.668814704031135 1106 26-27 2.4777836128895174 1106 28-29 2.0730981133033204 1106 30-31 2.1095549566404124 1106 32-33 2.236073427659864 1106 34-35 2.4829524827160867 1106 36-37 1.7259677167707999 1106 38-39 2.790828376250442 1106 40-41 2.08783035773137 1106 42-43 2.1887832545334867 1106 44-45 2.356342582246203 1106 46-47 2.709031664071283 1106 48-49 2.6638906986819215 1106 50-51 2.610833466773876 1106 52-53 2.603990257255699 1106 54-55 2.5602083385106376 1106 56-57 2.86321484248257 1106 58-59 2.797014773963646 1106 60-61 2.841158847618548 1106 62-63 3.022903776071246 1106 64-65 2.9204977502272236 1106 66-67 2.540297701004622 1106 68-69 2.7429170591156264 1106 70-71 2.756752508017346 1106 72-73 2.8257461176626606 1106 74-75 2.8436029851171796 1106 76-77 2.9601010179809712 1106 78-79 2.7770417847688833 1106 80-81 2.6999918873820157 1106 82-83 2.859512725037977 1106 84-85 2.7887307930221716 1106 86-87 2.900271782768886 1106 88-89 2.943827201821904 1106 90-91 2.734382368670712 1106 92-93 2.791972327448711 1106 94-95 2.738482808401079 1106 96-97 2.5292103098359444 1106 98-99 2.595615005756361 1106 100-101 2.2654464512697476 1107 1 2.998093757572068 1107 2 3.349445667823826 1107 3 0.017807933867384662 1107 4 1.0575797166357788 1107 5 1.5042675387572935 1107 6 1.8321700854038596 1107 7 1.9304385969362272 1107 8 1.8235472276710425 1107 9 2.1846181251695356 1107 10-11 1.63056886695367 1107 12-13 2.236401089452851 1107 14-15 2.5163453003342617 1107 16-17 1.034249720403281 1107 18-19 0.08033837878868866 1107 20-21 -1.3938897792681146 1107 22-23 0.4454681106633984 1107 24-25 0.9828705260238166 1107 26-27 1.1457749502672243 1107 28-29 2.0018538861076074 1107 30-31 2.510410561384358 1107 32-33 1.2266076682457516 1107 34-35 0.7983106356919869 1107 36-37 0.8883932851699932 1107 38-39 2.059862165942743 1107 40-41 2.266908075390006 1107 42-43 1.3904087600808417 1107 44-45 1.0183564028178829 1107 46-47 1.861525652156761 1107 48-49 1.3307897463400096 1107 50-51 1.75406155744448 1107 52-53 1.2630174201785849 1107 54-55 1.7139342225353396 1107 56-57 2.281046283429177 1107 58-59 2.2720917674598446 1107 60-61 2.261291075579418 1107 62-63 2.1072008261341004 1107 64-65 1.358523895223673 1107 66-67 1.959432224423761 1107 68-69 2.2338707332152055 1107 70-71 0.8592283237248388 1107 72-73 1.7693195518321971 1107 74-75 2.214652815690492 1107 76-77 2.329781122642938 1107 78-79 2.2960991438987968 1107 80-81 2.2055126353188363 1107 82-83 2.0197308618910164 1107 84-85 2.2288471372316003 1107 86-87 2.4004627293721548 1107 88-89 2.422359987251209 1107 90-91 2.354718181918784 1107 92-93 2.407532460257368 1107 94-95 2.316259732213725 1107 96-97 2.2447592386220823 1107 98-99 2.0362964699308144 1107 100-101 1.732514754816778 1108 1 2.355546919522208 1108 2 2.349947600684196 1108 3 1.7721138930111664 1108 4 1.628433229380441 1108 5 1.89750872308149 1108 6 -0.23373966790556366 1108 7 0.45323343449742026 1108 8 -0.6602465889210123 1108 9 1.203307270609642 1108 10-11 2.6267453811730697 1108 12-13 1.5272130653335445 1108 14-15 1.7598516748466828 1108 16-17 -0.061178693953113594 1108 18-19 0.25168841412992293 1108 20-21 1.055176862965105 1108 22-23 -0.8315791942398754 1108 24-25 0.9591236565595125 1108 26-27 1.1896312466798733 1108 28-29 1.7810700959560357 1108 30-31 0.49586018048433544 1108 32-33 0.7644151152120777 1108 34-35 1.0047245906820805 1108 36-37 -0.1979423955197248 1108 38-39 1.3408753687316448 1108 40-41 0.1354948621296188 1108 42-43 1.2962459984029628 1108 44-45 2.0765811381949 1108 46-47 1.9016609087516123 1108 48-49 1.8007529216412799 1108 50-51 2.052347638231936 1108 52-53 1.1645657155091804 1108 54-55 1.5134376126764444 1108 56-57 2.370259875408024 1108 58-59 2.3514704597467286 1108 60-61 1.4664474060243684 1108 62-63 1.4772634804793263 1108 64-65 1.2171669661592084 1108 66-67 1.5077836646883434 1108 68-69 2.0939218689342916 1108 70-71 2.364192677067418 1108 72-73 2.283771849885376 1108 74-75 2.385032232254627 1108 76-77 2.4411754872423614 1108 78-79 2.4383181374152088 1108 80-81 2.5033589140172303 1108 82-83 2.5285336228707997 1108 84-85 2.500740904084136 1108 86-87 2.287584119984473 1108 88-89 2.379400049381978 1108 90-91 2.3938138825043147 1108 92-93 2.442516273097148 1108 94-95 2.486879381942776 1108 96-97 2.469524023040522 1108 98-99 2.3213211215360054 1108 100-101 2.0179696096559088 1109 1 1.4548435294101694 1109 2 1.1728976689274901 1109 3 0.7578020617936936 1109 4 1.935333047092037 1109 5 1.8703829143931365 1109 6 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50-51 -0.6345379715519499 2312 52-53 -0.7846956346596876 2312 54-55 -0.7699452215231553 2312 56-57 0.2683077341026916 2312 58-59 0.008012955192782556 2312 60-61 0.5553899863290503 2312 62-63 -0.07173190074936997 2312 64-65 -1.624415202004922 2312 66-67 1.5135068422249027 2312 68-69 0.6802172660085013 2312 70-71 -2.2969740243022336 2312 72-73 -2.991142538497371 2312 74-75 -0.5204226191576531 2312 76-77 0.10951307005482036 2312 78-79 -0.08677441479969161 2312 80-81 -1.5329134350345903 2312 82-83 0.46184037240557885 2312 84-85 1.734458065563036 2312 86-87 2.0337927857789815 2312 88-89 2.4337251548332297 2312 90-91 2.835213041513409 2312 92-93 3.0647472038051013 2312 94-95 2.3209658355985727 2312 96-97 1.7583295592802646 2312 98-99 0.9741359267474774 2312 100-101 1.819531047695362 2313 1 -7.7676050695609895 2313 2 -4.3186998002274954 2313 3 -1.7853903768369825 2313 4 0.25312298918702325 2313 5 0.6031066487889412 2313 6 -2.907298504255465 2313 7 -1.149994326801341 2313 8 -2.5522715569792354 2313 9 0.48660492546081713 2313 10-11 -1.6970479450433338 2313 12-13 -2.467893260295611 2313 14-15 -3.6407053597555006 2313 16-17 -3.68000848976963 2313 18-19 -1.085845661791133 2313 20-21 -3.0080839314173957 2313 22-23 -1.668444603498017 2313 24-25 -2.9161798294251895 2313 26-27 -2.2062347398129596 2313 28-29 -2.9331825977537385 2313 30-31 -4.628215946952572 2313 32-33 -5.8743605468723565 2313 34-35 -4.7550718574796775 2313 36-37 -1.9894895658502385 2313 38-39 -1.3453481718120592 2313 40-41 -3.3955761239786 2313 42-43 -2.6743858890919334 2313 44-45 -3.014579603842485 2313 46-47 -1.5835542534463336 2313 48-49 -2.5963409549805903 2313 50-51 -3.244774318387286 2313 52-53 -0.5339938244041313 2313 54-55 -1.94510617992432 2313 56-57 -2.3624001008396753 2313 58-59 -1.3902209567171546 2313 60-61 -1.6968327128997345 2313 62-63 -3.8415414248272945 2313 64-65 -5.291679483784911 2313 66-67 -4.0692808561412015 2313 68-69 -2.784539960092925 2313 70-71 -1.7855031646539494 2313 72-73 -1.6193601017119619 2313 74-75 -2.5032318297890335 2313 76-77 -2.5175130205340466 2313 78-79 -2.944693026416253 2313 80-81 -3.7366665268356023 2313 82-83 -1.527461894335861 2313 84-85 -1.2128035357949614 2313 86-87 -2.920123370245376 2313 88-89 -3.7095463753589364 2313 90-91 -3.4488478886118124 2313 92-93 -1.6886128742527955 2313 94-95 -0.8299123313935954 2313 96-97 -0.7374256824947363 2313 98-99 -0.6529826164807453 2313 100-101 -0.3569677732007843 2314 1 -7.882055579592819 2314 2 -3.9056155782679234 2314 3 -25.120509823141962 2314 4 -13.544616980772606 2314 5 -7.045681725715724 2314 6 -5.519559152245428 2314 7 -4.520038385033214 2314 8 -3.638938961935718 2314 9 -3.7338533900309336 2314 10-11 -14.622401233730951 2314 12-13 -13.37051811416729 2314 14-15 -5.303769943916809 2314 16-17 -3.5776102563438172 2314 18-19 -3.571956622688532 2314 20-21 -4.753067914742807 2314 22-23 -4.644017137349799 2314 24-25 -5.437563627254015 2314 26-27 -4.144104300453847 2314 28-29 -3.5924727894719624 2314 30-31 -4.16999985013241 2314 32-33 -4.294284358816029 2314 34-35 -3.488177092771984 2314 36-37 -4.146145068295301 2314 38-39 -1.3998100192132377 2314 40-41 -2.6900160806297144 2314 42-43 -4.569416843187316 2314 44-45 -5.6317762067141075 2314 46-47 -4.713450786749121 2314 48-49 -3.4852494605108824 2314 50-51 -3.1248254279470054 2314 52-53 -1.1696156835306155 2314 54-55 -3.6158198578805774 2314 56-57 -3.384000891896399 2314 58-59 -1.3758032076612565 2314 60-61 -4.338908083150304 2314 62-63 -5.135045926643389 2314 64-65 -4.556557458035453 2314 66-67 -5.703571203997061 2314 68-69 -4.842629684444244 2314 70-71 -5.523380676889428 2314 72-73 -4.67030425709445 2314 74-75 -3.9784257489213175 2314 76-77 -3.677270584234517 2314 78-79 -4.837643084766029 2314 80-81 -7.221045553250686 2314 82-83 -6.764679515617864 2314 84-85 -5.369608117944505 2314 86-87 -4.716908184101136 2314 88-89 -3.7889615807677686 2314 90-91 -5.5774708212035655 2314 92-93 -5.635470351770373 2314 94-95 -5.928512510543124 2314 96-97 -5.162290831625471 2314 98-99 -5.979149511717676 2314 100-101 -5.1545920235941605 2315 1 -24.5695697938298 2315 2 -24.71298423871823 2315 3 -13.422017360830404 2315 4 -28.95830813383151 2315 5 -15.67124451599395 2315 6 -12.382301337129366 2315 7 -9.507044143870612 2315 8 -8.589616637534338 2315 9 -5.977653385314067 2315 10-11 -29.364384404074368 2315 12-13 -29.10397274232285 2315 14-15 -23.820899547524398 2315 16-17 -14.152542311122225 2315 18-19 -9.14395193428902 2315 20-21 -6.98357990363677 2315 22-23 -3.8698656097654265 2315 24-25 -5.580063216606074 2315 26-27 -5.57403565757231 2315 28-29 -17.626018440692064 2315 30-31 -16.56452751045316 2315 32-33 -9.64669200770409 2315 34-35 -7.499269106690271 2315 36-37 -5.470565228724883 2315 38-39 -5.569698423751696 2315 40-41 -8.982993257954718 2315 42-43 -8.46080986490475 2315 44-45 -5.639215142248148 2315 46-47 -4.375953502324222 2315 48-49 -5.202948272556512 2315 50-51 -3.5762238543212312 2315 52-53 -7.408018216799849 2315 54-55 -7.345618810258198 2315 56-57 -8.102512376480398 2315 58-59 -7.823333361583842 2315 60-61 -5.230052430395656 2315 62-63 -3.878811948017031 2315 64-65 -4.934267461731029 2315 66-67 -7.269834457328244 2315 68-69 -6.93471147263136 2315 70-71 -7.690235221699819 2315 72-73 -7.042333189358889 2315 74-75 -7.666004378521858 2315 76-77 -7.491134884751723 2315 78-79 -7.36750955379528 2315 80-81 -8.376865226804014 2315 82-83 -7.464138442148297 2315 84-85 -6.88437214524706 2315 86-87 -7.283391332782289 2315 88-89 -6.639515106142696 2315 90-91 -6.089958258908974 2315 92-93 -5.995315637495626 2315 94-95 -6.123439400663916 2315 96-97 -6.124733168186813 2315 98-99 -5.958057713902541 2315 100-101 -5.8182000340809825 2316 1 -1.0287559243378652 2316 2 1.5887253687944103 2316 3 1.7261070803013325 2316 4 2.1873696894287846 2316 5 -1.3817633228425876 2316 6 -2.501890373086905 2316 7 -2.924554280636791 2316 8 -3.66612685097045 2316 9 -3.179225239946554 2316 10-11 -2.7935922056635825 2316 12-13 -3.651528725949145 2316 14-15 -2.0393750324507245 2316 16-17 -2.3001864376718366 2316 18-19 -4.047766065470729 2316 20-21 -1.6570724126982874 2316 22-23 -2.13400617996313 2316 24-25 -2.2337492803991985 2316 26-27 -2.5789064533347172 2316 28-29 -1.5889121179745693 2316 30-31 0.9736176788605242 2316 32-33 -1.3562133079205587 2316 34-35 -6.906097482749587 2316 36-37 -2.780106526923955 2316 38-39 -0.8429485260241059 2316 40-41 -0.7417047278757387 2316 42-43 -1.022729608534572 2316 44-45 -0.37354194338383095 2316 46-47 0.701574485226427 2316 48-49 1.0811915727418224 2316 50-51 1.202899060341835 2316 52-53 0.9418292463328548 2316 54-55 -0.7627218373785638 2316 56-57 0.47862270043208355 2316 58-59 -3.0493750249275955 2316 60-61 -3.065014871478329 2316 62-63 -4.630846863746655 2316 64-65 -4.825043304256045 2316 66-67 -2.3072949247376364 2316 68-69 -1.9605370026631483 2316 70-71 -2.777139711092918 2316 72-73 -2.761515561170228 2316 74-75 -2.523535747469566 2316 76-77 -2.152334767007826 2316 78-79 -2.8044307961738006 2316 80-81 -2.936109635261378 2316 82-83 -3.827277358717442 2316 84-85 -2.999969257177572 2316 86-87 -2.444225492856571 2316 88-89 -3.663991597348147 2316 90-91 -3.8977209961553925 2316 92-93 -4.228488072135608 2316 94-95 -5.696399284688818 2316 96-97 -5.500324238922579 2316 98-99 -5.540418684748496 2316 100-101 -5.2754344362870516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63219.0 3 23930.0 4 21873.0 5 22758.0 6 27125.0 7 33473.0 8 41091.0 9 49483.0 10 58392.0 11 67501.0 12 74966.0 13 82424.0 14 89134.0 15 96094.0 16 101970.0 17 104260.0 18 101546.0 19 104460.0 20 112757.0 21 121397.0 22 132539.0 23 145091.0 24 158043.0 25 172276.0 26 186843.0 27 201570.0 28 217914.0 29 234659.0 30 251582.0 31 267936.0 32 284754.0 33 298592.0 34 305445.0 35 299616.0 36 264512.0 37 163598.0 38 32251.0 39 246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.640258677541574 19.148411099028497 14.50083709951496 22.71049312391497 2 15.644659612210452 20.33267784647677 40.3116017486114 23.711060792701378 3 19.34293364791809 24.97320711264536 29.892018620452358 25.791840618984192 4 12.719373040121203 16.98179420454717 37.51404569317421 32.78478706215742 5 13.762859087727822 37.54430173435897 33.98492302923196 14.707916148681255 6 31.482520011399735 36.02355306024535 17.932205920037426 14.561721008317493 7 28.48529034032385 33.057661722233746 20.753625699049866 17.703422238392545 8 26.517015761407496 34.303710328774656 19.89028669606433 19.288987213753522 9 26.943272604328477 16.85665478671268 20.662817207644657 35.53725540131419 10-11 25.10147605413059 26.67264016986481 27.217348061290664 21.00853571471394 12-13 26.34057233135233 24.212881760199604 27.590625571925152 21.85592033652291 14-15 23.292951602567346 25.40234964958139 26.453590446312734 24.85110830153853 16-17 22.262076642824862 28.6965898126952 26.868654937253233 22.172678607226704 18-19 22.00338173352962 27.02532787487267 29.680948689618177 21.290341701979543 20-21 23.1623825492771 25.995553711808178 29.598839876529258 21.243223862385463 22-23 22.634964507733045 26.17262051362481 29.432530050598555 21.759884928043586 24-25 22.30961387752062 26.253650734979527 29.749669735226046 21.6870656522738 26-27 22.171847123595626 26.52462106935366 29.59650254883533 21.707029258215385 28-29 21.914961189601904 26.740581397342805 29.683378591991268 21.661078821064024 30-31 22.098795345336466 26.441398828024496 29.66076590692271 21.79903991971632 32-33 22.007502824443534 26.474826853672756 29.54918146557913 21.968488856304575 34-35 22.6130014589658 26.553856485009632 29.26902249091678 21.564119565107784 36-37 22.44613749400646 26.376712545521215 29.266640131863397 21.91050982860893 38-39 22.15287158508035 26.660739781079794 29.435049025499026 21.751339608340825 40-41 22.411502271616794 26.462231968932524 29.33071276811841 21.795552991332272 42-43 22.232737637970327 26.557860894924545 29.137402955611524 22.0719985114936 44-45 22.29963532489146 26.717113456563414 28.924450002615377 22.058801215929748 46-47 22.35561499061302 26.87518188675026 28.49909674353456 22.270106379102156 48-49 22.481156243643984 26.89887620928394 28.40958187125248 22.210385675819595 50-51 22.35851196104734 27.18392433958867 28.226775517818066 22.230788181545922 52-53 22.477295318273054 27.322295813676984 27.974171476879018 22.226237391170944 54-55 22.208726324637716 27.09166991606952 28.134987918613646 22.564615840679114 56-57 22.26240744769877 27.457248432927646 28.002819367283898 22.27752475208969 58-59 22.379422794798494 27.094771090122862 28.06563828560561 22.460167829473036 60-61 22.39434681117307 27.137779709249806 28.292803528871254 22.175069950705875 62-63 22.2252145169192 27.09243582060233 28.29126907854048 22.391080583937992 64-65 22.10941660924134 27.11374446940195 28.168011603775618 22.608827317581092 66-67 22.064152166718102 27.33356914921436 27.862198621115873 22.740080062951662 68-69 22.238241984261002 27.10121563113144 27.99894885698177 22.66159352762579 70-71 22.053826344875034 27.137868120104685 28.026698758254398 22.781606776765887 72-73 22.263059852547258 27.028194004911633 27.99855001176543 22.71019613077568 74-75 22.081235354082985 27.340556073082066 27.728900643896164 22.849307928938785 76-77 22.22801877002959 27.173661321182152 27.764346630585152 22.833973278203104 78-79 22.144338940443607 27.287288644825235 27.66015836552098 22.908214049210173 80-81 22.016147193215563 27.38846188222805 27.659206241970217 22.936184682586166 82-83 22.20955124123077 27.333130087247692 27.524809187175762 22.932509484345772 84-85 22.101597777439412 27.24444921484003 27.492395517935993 23.16155748978456 86-87 22.00482630908567 27.145617287408214 27.803126766945024 23.046429636561093 88-89 22.145911811288084 27.09280730668302 27.52061065013468 23.24067023189421 90-91 21.99238767861941 27.2678328348683 27.618484087843942 23.121295398668348 92-93 22.203173803184427 26.989936896939653 27.66628501049721 23.14060428937871 94-95 21.945443417207088 26.954753085266645 27.60730392632341 23.492499571202853 96-97 22.055006254865464 26.85137874256776 27.524005287591454 23.569609714975325 98-99 21.884948992717955 26.826568247248815 27.3505137695433 23.93796899048993 100-101 21.893874078916216 26.86304195074235 27.525785548939602 23.717298421401832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4550.0 1 2792.5 2 984.5 3 974.5 4 974.0 5 1008.0 6 1336.0 7 2256.0 8 3566.5 9 4377.5 10 4318.5 11 4126.0 12 4209.5 13 4458.5 14 4922.0 15 5413.0 16 5997.5 17 6658.0 18 7360.0 19 8247.5 20 9401.5 21 10765.5 22 12452.5 23 14885.0 24 17931.5 25 22157.5 26 28237.5 27 35493.5 28 42612.5 29 50347.0 30 59815.0 31 70048.0 32 80649.0 33 90938.5 34 101847.0 35 112528.5 36 122815.0 37 132915.5 38 140396.5 39 144955.5 40 149986.0 41 157475.0 42 164660.0 43 170559.5 44 176010.5 45 180489.5 46 184289.5 47 189650.0 48 196930.0 49 198883.0 50 192880.5 51 185402.5 52 178458.0 53 171847.5 54 163783.5 55 152192.0 56 138863.5 57 124265.5 58 109175.5 59 94752.5 60 80334.5 61 65385.5 62 51647.0 63 40168.5 64 30466.0 65 23221.0 66 17623.0 67 13117.5 68 9466.5 69 6806.5 70 5014.0 71 3775.0 72 2882.5 73 2124.0 74 1536.0 75 1135.5 76 823.5 77 555.0 78 361.0 79 249.5 80 189.5 81 134.0 82 91.5 83 68.5 84 46.0 85 26.0 86 18.0 87 14.0 88 11.0 89 7.0 90 4.5 91 4.5 92 3.5 93 2.0 94 2.0 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.580924048714738 2 2.6710359458618793 3 1.0231849612786421 4 0.9669373041002368 5 0.1743458044551494 6 0.09323433001284066 7 0.09508864838135951 8 0.10310408907108619 9 0.09807948445961574 10-11 1.888234449646284 12-13 1.4581422521394447 14-15 0.5426273896780265 16-17 0.09138998109791599 18-19 0.09714235582176212 20-21 0.12358134675354714 22-23 0.13908384709250854 24-25 0.15144596954930095 26-27 0.14843519456385634 28-29 0.5851969565252068 30-31 0.18088576601293635 32-33 0.16228276560618266 34-35 0.14596277007249786 36-37 0.1356543550561081 38-39 0.45492610640995984 40-41 0.1240698499796623 42-43 0.12505682588548686 44-45 0.12916424076629207 46-47 0.1406191429460134 48-49 0.1547159503281944 50-51 0.16791550688689855 52-53 0.1855115924806393 54-55 0.18487354745061133 56-57 0.15579265131636666 58-59 0.1388246412990597 60-61 0.11762958295781724 62-63 0.12278379046601214 64-65 0.11795857492642543 66-67 0.1148879832194157 68-69 0.11037182074124881 70-71 0.12607371015209398 72-73 0.13964213649378304 74-75 0.15004027659252053 76-77 0.16965019181228716 78-79 0.17170389925268975 80-81 0.17082658733640127 82-83 0.16456577047925158 84-85 0.15118676375585205 86-87 0.15072816889051946 88-89 0.14777721062663998 90-91 0.15513466737915027 92-93 0.14599267843328043 94-95 0.200176658717689 96-97 0.19971806385235638 98-99 0.21708485201343083 100-101 0.24282598119362273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5015320.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.16213506340412 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37873043999063 39.5413211349531 2 12.187537460922316 12.958310251756991 3 5.0686841098117466 8.083862077186271 4 2.6788937771297054 5.696628512011289 5 1.5531505202810243 4.128439886648811 6 1.0357350448885436 3.303713180775941 7 0.6994251281649637 2.6028053191171177 8 0.47996229662666534 2.0412656350886733 9 0.3413484406886791 1.6332130716816555 >10 1.4789044980981576 13.025942792994199 >50 0.06194898920120831 2.2571501733061514 >100 0.03350099848307201 3.2041205002024933 >500 0.0017030317851719169 0.5896049864395135 >1k 3.5644794606585817E-4 0.2944757560568455 >5k 7.921065468086412E-5 0.35389307219970423 >10k+ 3.960532734043206E-5 0.2852536495812466 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13548 0.27013231458810205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9951 0.19841206543151782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6857 0.13672108659068613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 2 0.0 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 3 0.0 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 4 0.0 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 5 3.987781437674964E-5 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 6 9.96945359418741E-5 0.0 0.0 1.993890718837482E-5 1.993890718837482E-5 7 9.96945359418741E-5 0.0 0.0 3.987781437674964E-5 1.993890718837482E-5 8 9.96945359418741E-5 1.993890718837482E-5 0.0 3.987781437674964E-5 3.987781437674964E-5 9 1.1963344313024892E-4 1.993890718837482E-5 0.0 5.981672156512446E-5 5.981672156512446E-5 10-11 1.1963344313024892E-4 1.993890718837482E-5 0.0 5.981672156512446E-5 5.981672156512446E-5 12-13 1.2960289672443633E-4 1.993890718837482E-5 0.0 6.978617515931187E-5 5.981672156512446E-5 14-15 1.5951125750699856E-4 1.993890718837482E-5 0.0 1.2960289672443633E-4 9.96945359418741E-5 16-17 1.5951125750699856E-4 1.993890718837482E-5 0.0 1.3957235031862374E-4 1.1963344313024892E-4 18-19 2.5920579344887267E-4 1.993890718837482E-5 0.0 1.4954180391281115E-4 1.6948071110118597E-4 20-21 3.1902251501399713E-4 1.993890718837482E-5 0.0 1.7945016469537339E-4 2.0935852547793562E-4 22-23 3.1902251501399713E-4 1.993890718837482E-5 0.0 2.891141542314349E-4 2.3926688626049785E-4 24-25 4.3865595814424605E-4 1.993890718837482E-5 0.0 3.88808690173309E-4 2.990836078256223E-4 26-27 4.3865595814424605E-4 1.993890718837482E-5 0.0 7.77617380346618E-4 2.990836078256223E-4 28-29 4.984726797093705E-4 1.993890718837482E-5 0.0 0.0018941961828956078 3.1902251501399713E-4 30-31 5.58289401274495E-4 1.993890718837482E-5 0.0 0.0052738409513251394 3.1902251501399713E-4 32-33 5.682588548686824E-4 1.993890718837482E-5 0.0 0.012152763931314453 3.1902251501399713E-4 34-35 6.579839372163691E-4 1.993890718837482E-5 0.0 0.023308582503210162 3.1902251501399713E-4 36-37 7.576784731582432E-4 1.993890718837482E-5 0.0 0.04193152181715225 3.1902251501399713E-4 38-39 7.77617380346618E-4 1.993890718837482E-5 0.0 0.07204924112519241 3.1902251501399713E-4 40-41 7.875868339408054E-4 5.981672156512446E-5 0.0 0.10706196214797858 3.3896142220237195E-4 42-43 8.27464648317555E-4 5.981672156512446E-5 0.0 0.1416659355734031 3.5890032939074677E-4 44-45 8.773119162884921E-4 5.981672156512446E-5 0.0 0.185282295047973 3.987781437674964E-4 46-47 9.670369986361787E-4 5.981672156512446E-5 0.0 0.2388182608487594 4.585948653326209E-4 48-49 9.96945359418741E-4 5.981672156512446E-5 0.0 0.296461641530351 5.58289401274495E-4 50-51 9.96945359418741E-4 5.981672156512446E-5 0.0 0.3604455946978458 5.58289401274495E-4 52-53 9.96945359418741E-4 5.981672156512446E-5 0.0 0.4256956684718024 5.981672156512446E-4 54-55 9.96945359418741E-4 5.981672156512446E-5 0.0 0.49830319899826925 6.480144836221817E-4 56-57 0.0010567620809838654 5.981672156512446E-5 0.0 0.5849676590925404 6.579839372163691E-4 58-59 0.0010767009881722403 5.981672156512446E-5 0.0 0.6807441997718989 6.579839372163691E-4 60-61 0.0010767009881722403 5.981672156512446E-5 0.0 0.7859817519121413 6.779228444047439E-4 62-63 0.0010767009881722403 5.981672156512446E-5 0.0 0.895875038880869 6.978617515931187E-4 64-65 0.0010767009881722403 5.981672156512446E-5 0.0 1.0113412504087476 6.978617515931187E-4 66-67 0.001096639895360615 5.981672156512446E-5 0.0 1.1436558385108029 7.078312051873061E-4 68-69 0.001096639895360615 5.981672156512446E-5 0.0 1.2958594865332622 7.178006587814935E-4 70-71 0.0011365177097373649 5.981672156512446E-5 0.0 1.4652105947377236 7.576784731582432E-4 72-73 0.001166426070519927 5.981672156512446E-5 0.0 1.6381108284217158 7.77617380346618E-4 74-75 0.0011963344313024892 5.981672156512446E-5 0.0 1.8184781828477545 7.77617380346618E-4 76-77 0.001216273338490864 5.981672156512446E-5 0.0 2.016710798114577 7.875868339408054E-4 78-79 0.001216273338490864 5.981672156512446E-5 0.0 2.24168148792101 7.975562875349928E-4 80-81 0.0013558456888094878 7.975562875349928E-5 0.0 2.488664731263409 7.975562875349928E-4 82-83 0.0014156624103746122 9.96945359418741E-5 0.0 2.7452585278706048 8.374341019117425E-4 84-85 0.0014854485855339242 9.96945359418741E-5 0.0 3.0022610720751617 8.474035555059299E-4 86-87 0.001535295853504861 1.0966398953606151E-4 0.0 3.270269893047702 8.573730091001173E-4 88-89 0.001535295853504861 1.1963344313024892E-4 1.993890718837482E-5 3.5663526953414735 8.573730091001173E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22745 0.0 36.694424 1 GGTATCA 14275 0.0 36.325153 1 TATCAAC 28395 0.0 28.940897 2 ATCAACG 28910 0.0 28.558104 3 TCAACGC 29160 0.0 28.422606 4 CAACGCA 29295 0.0 27.76923 5 AACGCAG 29795 0.0 27.149601 6 GTACATG 20140 0.0 25.44601 1 TACATGG 20345 0.0 24.32677 2 ACGCAGA 33005 0.0 23.965258 7 CGCAGAG 33375 0.0 23.481588 8 ACATGGG 21570 0.0 22.608547 3 GAGTACT 16135 0.0 22.308784 12-13 GCAGAGT 33445 0.0 21.87854 9 GTACTTT 17690 0.0 20.706055 14-15 AGAGTAC 27540 0.0 20.44335 10-11 AGTACTT 17110 0.0 20.010971 12-13 CATGGGA 13315 0.0 19.411531 4 GTATAGG 1340 0.0 19.216686 1 GTGTAGC 3390 0.0 18.46865 1 >>END_MODULE