FastQCFastQC Report
Fri 27 May 2016
SRR939486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015320
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195840.3904835583771325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168330.3356316247019133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112230.2237743553751306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158850.037.8693161
GTATCAA250950.033.279491
ATCAACG314350.026.2538763
TATCAAC315100.026.2423322
TCAACGC315550.026.1167354
CAACGCA322200.025.5491375
AACGCAG327100.025.2243926
GTACATG229750.023.3724981
TACATGG233850.022.3893172
ACGCAGA371800.022.0897377
CGCAGAG373350.021.9345238
ACATGGG238650.020.9199943
GAGTACT188550.020.88064412-13
AGTACTT195600.020.37040112-13
GCAGAGT392050.020.2835339
AGAGTAC325450.019.87846610-11
GTACTTT202100.019.42203714-15
CAGAGTA384050.017.37617510-11
CATGGGA145850.017.1530574
ACCGTCG4700.017.1515398
Produced by FastQC (version 0.11.5)