##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5015320 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90029449765917 31.0 31.0 31.0 28.0 34.0 2 31.155509718223364 31.0 31.0 34.0 28.0 34.0 3 31.164802046529434 31.0 31.0 34.0 28.0 34.0 4 35.073863881068405 35.0 35.0 37.0 32.0 37.0 5 34.6476406291124 35.0 35.0 37.0 32.0 37.0 6 34.70077163570819 35.0 35.0 37.0 31.0 37.0 7 34.53976416260578 35.0 35.0 37.0 30.0 37.0 8 34.43605871609389 35.0 35.0 37.0 30.0 37.0 9 35.94644469345924 37.0 35.0 39.0 30.0 39.0 10-11 35.73972209151161 37.0 35.0 39.0 30.0 39.0 12-13 35.72345214263497 37.0 35.0 39.0 30.0 39.0 14-15 36.566567836150035 38.0 35.5 40.0 30.5 41.0 16-17 36.48759191836214 38.0 35.0 40.0 30.0 41.0 18-19 36.48376893199237 38.0 35.0 40.0 30.0 41.0 20-21 36.353125922174456 38.0 34.5 40.0 29.5 41.0 22-23 36.19613623856504 38.0 34.0 40.0 29.0 41.0 24-25 36.03418027164767 38.0 34.0 40.0 29.0 41.0 26-27 35.57373856503673 38.0 34.0 40.0 27.0 41.0 28-29 35.370364702551385 38.0 34.0 40.0 27.0 41.0 30-31 35.08761165788025 37.5 33.0 40.0 27.0 41.0 32-33 34.85852637518643 37.0 33.0 40.0 26.0 41.0 34-35 35.13076403898455 38.0 33.0 40.0 27.0 41.0 36-37 34.97751529314181 38.0 33.0 40.0 26.0 41.0 38-39 34.8002815373695 38.0 33.0 40.0 25.0 41.0 40-41 34.654148289640545 38.0 33.0 40.0 25.0 41.0 42-43 34.502666629447376 37.0 33.0 40.0 25.0 41.0 44-45 34.114677228970436 37.0 33.0 40.0 24.0 41.0 46-47 33.727121100946704 37.0 32.0 40.0 23.0 41.0 48-49 33.3408301962786 37.0 31.5 40.0 22.0 41.0 50-51 33.11014880406435 37.0 31.0 39.5 21.0 41.0 52-53 33.007914749208425 37.0 31.0 39.0 21.0 40.0 54-55 32.67789951588334 36.0 31.0 39.0 19.5 40.0 56-57 32.17045353038291 36.0 30.5 39.0 17.0 40.0 58-59 31.713620566583987 35.0 30.0 39.0 14.0 40.0 60-61 31.340836277645295 35.0 29.0 38.0 13.0 40.0 62-63 31.19817558999226 35.0 29.0 38.0 13.0 40.0 64-65 30.747060406913217 34.0 28.5 38.0 8.5 40.0 66-67 29.898374480591468 34.0 27.0 37.5 7.5 39.5 68-69 26.503205877192283 31.0 19.5 36.0 2.0 39.0 70-71 25.221873978131008 30.0 14.0 35.0 2.0 38.0 72-73 26.195508262683138 31.0 20.0 35.0 2.0 38.0 74-75 26.91742092229409 31.0 23.0 35.0 2.0 37.5 76-77 26.561305260681273 30.5 23.5 34.0 2.0 36.0 78-79 27.658460875876315 32.0 26.0 35.0 2.0 37.0 80-81 27.673009199811776 32.0 26.0 35.0 2.0 36.5 82-83 27.352255788264756 32.0 26.0 35.0 2.0 36.0 84-85 26.995121547578222 32.0 25.5 35.0 2.0 36.0 86-87 26.557229548662896 32.0 25.0 34.0 2.0 35.0 88-89 26.08338590558529 31.5 24.0 34.0 2.0 35.0 90-91 25.49879060558449 31.0 22.0 34.0 2.0 35.0 92-93 24.93750588197762 31.0 19.5 34.0 2.0 35.0 94-95 24.11140555338443 31.0 16.5 34.0 2.0 35.0 96-97 23.444245631385435 30.5 4.5 34.0 2.0 35.0 98-99 22.594621280396865 30.0 2.0 34.0 2.0 35.0 100-101 21.16502665831891 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17905829603578738 1101 2 0.1914927424134838 1101 3 0.2903963699994847 1101 4 0.32352988643646086 1101 5 0.4739925759623702 1101 6 0.3656194392201826 1101 7 0.4997011999509553 1101 8 0.5653796088170395 1101 9 0.6002511513870488 1101 10-11 0.7050792234167176 1101 12-13 0.7021957581352822 1101 14-15 0.7366335369200314 1101 16-17 0.7124621282198191 1101 18-19 0.57614451604001 1101 20-21 0.7376125126991795 1101 22-23 0.7470329087159939 1101 24-25 0.7572065646689197 1101 26-27 0.8394952319010685 1101 28-29 0.7084519731647845 1101 30-31 0.5305935167677021 1101 32-33 0.8020316028323933 1101 34-35 0.6445513366677531 1101 36-37 0.7339485226427982 1101 38-39 0.8545580934355144 1101 40-41 0.8428666855929805 1101 42-43 0.44895822131915963 1101 44-45 0.6047868540001176 1101 46-47 0.6360435183097195 1101 48-49 0.6760498517337084 1101 50-51 0.39384106920318374 1101 52-53 0.12180602643592664 1101 54-55 0.273459904969819 1101 56-57 0.2446067018460667 1101 58-59 0.2970215789447188 1101 60-61 0.1402060140561936 1101 62-63 -0.06074252708662087 1101 64-65 -0.0011240854661132005 1101 66-67 -0.26935541686094666 1101 68-69 -7.168956879872528 1101 70-71 -7.893914128804106 1101 72-73 -4.902587395507258 1101 74-75 -2.968879078980656 1101 76-77 -0.41038990908824147 1101 78-79 -0.05969918532106888 1101 80-81 -0.15511523713923836 1101 82-83 -0.4868054277356535 1101 84-85 -0.455880509593829 1101 86-87 -0.47643226109870795 1101 88-89 -0.5071051647488325 1101 90-91 -0.5293534875390158 1101 92-93 -0.45937726731632367 1101 94-95 -0.3355354354560873 1101 96-97 -0.5043145987518187 1101 98-99 -0.6171107854747611 1101 100-101 -0.6481693684736705 1102 1 0.3292047846848476 1102 2 0.3358095492183857 1102 3 0.35885213763604895 1102 4 0.32143733856376144 1102 5 0.49898472375885916 1102 6 0.4228092256917364 1102 7 0.4252272975860265 1102 8 0.557977426676814 1102 9 0.5597193641225502 1102 10-11 0.5592563382339506 1102 12-13 0.5580210633414211 1102 14-15 0.7682956810607351 1102 16-17 0.6136057179898202 1102 18-19 0.5376738678341795 1102 20-21 0.6348464774698144 1102 22-23 0.6982493566365733 1102 24-25 0.685918854783381 1102 26-27 0.6264821998177368 1102 28-29 0.6269203558168215 1102 30-31 0.5692768180915024 1102 32-33 0.746042044975809 1102 34-35 0.4031704816271571 1102 36-37 0.5520502974982335 1102 38-39 0.7424312018268111 1102 40-41 0.5594670161578748 1102 42-43 0.5417686922968983 1102 44-45 0.5006450097363384 1102 46-47 0.6843487295983621 1102 48-49 0.7068212213124667 1102 50-51 0.44582677561830053 1102 52-53 0.15124329376612167 1102 54-55 -0.037268007167888584 1102 56-57 0.004519272065358848 1102 58-59 0.2359273164597191 1102 60-61 0.41026826529581584 1102 62-63 0.18683822188486943 1102 64-65 0.07563699643880639 1102 66-67 -0.022089270928390192 1102 68-69 -6.940305347791774 1102 70-71 -7.907789616026896 1102 72-73 -1.6352783070322339 1102 74-75 0.014514926569948017 1102 76-77 0.35610718430921295 1102 78-79 0.20380789196005722 1102 80-81 -0.0054228890471925695 1102 82-83 -0.10514045165269437 1102 84-85 -0.38398676406701426 1102 86-87 -0.27813142644809474 1102 88-89 -0.4473414002222569 1102 90-91 -0.6479299988951048 1102 92-93 -0.4464594914722788 1102 94-95 -0.26174583331822987 1102 96-97 -0.5815298212137563 1102 98-99 -0.4637060185436077 1102 100-101 -0.5377231058650906 1103 1 0.38936973272521413 1103 2 0.2898565884712099 1103 3 0.3204911960493 1103 4 0.28829941755042654 1103 5 0.39007592619600473 1103 6 0.4410326771743698 1103 7 0.511438202595599 1103 8 0.5711933121952555 1103 9 0.6156263278159457 1103 10-11 0.5548104574254467 1103 12-13 0.519562916651914 1103 14-15 0.6742186785855111 1103 16-17 0.602440659716855 1103 18-19 0.6626074777511022 1103 20-21 0.6156159009533653 1103 22-23 0.6428143888545534 1103 24-25 0.6500832416396563 1103 26-27 0.7222086826966105 1103 28-29 0.6338975777551141 1103 30-31 0.5640417721445559 1103 32-33 0.6709405808865796 1103 34-35 0.6114339174767949 1103 36-37 0.789082936366249 1103 38-39 0.7992510917946447 1103 40-41 0.7103724265738549 1103 42-43 0.5245348288450202 1103 44-45 0.5748876617320562 1103 46-47 0.5897090398529983 1103 48-49 0.6430707077288105 1103 50-51 0.5157828830048814 1103 52-53 0.28050804238571203 1103 54-55 0.12105555554944658 1103 56-57 0.5006390848703788 1103 58-59 0.5577578789465711 1103 60-61 0.452146127585241 1103 62-63 0.16063092448736072 1103 64-65 0.20999549286626618 1103 66-67 0.03261966492626556 1103 68-69 -7.019064529665528 1103 70-71 -7.9539906001033 1103 72-73 -1.1828030843122939 1103 74-75 0.2514603814798093 1103 76-77 0.5118748540924543 1103 78-79 0.2187619450119982 1103 80-81 0.033172547323548685 1103 82-83 -0.11664121827025653 1103 84-85 -0.447025776382997 1103 86-87 -0.26376912198567837 1103 88-89 -0.10486828194268938 1103 90-91 -0.2201819505130196 1103 92-93 -0.22075475447721615 1103 94-95 0.14049840201833064 1103 96-97 0.1423159934551954 1103 98-99 0.23008437919562041 1103 100-101 0.29613082593099804 1104 1 0.29524210716040855 1104 2 0.2904357598899914 1104 3 0.23084427069321833 1104 4 0.22394353622491536 1104 5 0.5243640408448584 1104 6 0.37662885563533166 1104 7 0.41030909323089304 1104 8 0.4979990658254678 1104 9 0.5478827161008297 1104 10-11 0.41858552462555565 1104 12-13 0.41193616507407427 1104 14-15 0.5412459025923653 1104 16-17 0.519364192059065 1104 18-19 0.3831322588185415 1104 20-21 0.47910098014857994 1104 22-23 0.4621130738176191 1104 24-25 0.3634704289303343 1104 26-27 0.676279711989757 1104 28-29 0.5561795520336759 1104 30-31 0.5140748274246505 1104 32-33 0.7166409471766357 1104 34-35 0.3612676353375761 1104 36-37 0.7601248682965078 1104 38-39 0.6831668149107415 1104 40-41 0.7193773754789703 1104 42-43 0.5841250506506412 1104 44-45 0.6561460554599989 1104 46-47 0.6364266103100178 1104 48-49 0.7920080106459153 1104 50-51 0.6820126902852266 1104 52-53 0.5941342267268936 1104 54-55 0.416604625207583 1104 56-57 0.5412256048869253 1104 58-59 0.6468951964804113 1104 60-61 0.6984999558627258 1104 62-63 0.395797149634717 1104 64-65 0.4278250952441027 1104 66-67 0.37727469988909235 1104 68-69 -6.6826758625979075 1104 70-71 -4.249832004490063 1104 72-73 0.27917248160013486 1104 74-75 0.9065260528674166 1104 76-77 1.1232380927321373 1104 78-79 0.5747891026400929 1104 80-81 0.32720680680816727 1104 82-83 0.34349833803803875 1104 84-85 0.44535256778214105 1104 86-87 0.4345835114741803 1104 88-89 0.2977044312923631 1104 90-91 0.2731028659832404 1104 92-93 0.3537617030963638 1104 94-95 0.44384680802299314 1104 96-97 0.30885499835046204 1104 98-99 0.38475311902508835 1104 100-101 0.6522116564834661 1105 1 0.13313853675892062 1105 2 0.20588354527071928 1105 3 0.18988087332424897 1105 4 0.20278073475974168 1105 5 0.37627320888150706 1105 6 0.2553248057980042 1105 7 0.23493117558143695 1105 8 0.38776997087800424 1105 9 0.3482389019774317 1105 10-11 0.3713167503627517 1105 12-13 0.3464350365869606 1105 14-15 0.3871654810122749 1105 16-17 0.3174674468363108 1105 18-19 0.4501017899926296 1105 20-21 0.513124198094296 1105 22-23 0.4899666854654612 1105 24-25 0.4695354357873356 1105 26-27 0.5861990843409401 1105 28-29 0.5184107089726169 1105 30-31 0.4933068823276159 1105 32-33 0.5616804621586766 1105 34-35 0.5933448732863056 1105 36-37 0.6482381917481135 1105 38-39 0.5037071388429553 1105 40-41 0.6092889746048726 1105 42-43 0.5105305078199791 1105 44-45 0.6552245174308595 1105 46-47 0.7866619590519903 1105 48-49 0.7645980662649094 1105 50-51 0.5511317921386123 1105 52-53 0.4902480053556033 1105 54-55 0.6111739317928411 1105 56-57 0.7488564079876099 1105 58-59 0.8397062673269708 1105 60-61 0.8881985738328346 1105 62-63 0.6293780371518416 1105 64-65 0.6203108905226529 1105 66-67 0.7338553676430593 1105 68-69 -2.880937875067673 1105 70-71 -3.1523906802767954 1105 72-73 0.93499535770653 1105 74-75 1.3192649474913054 1105 76-77 1.26039456606156 1105 78-79 1.013688584794604 1105 80-81 0.8264488847373634 1105 82-83 0.7298020389931921 1105 84-85 0.6402781906672459 1105 86-87 0.7418619761839302 1105 88-89 0.645389614364209 1105 90-91 0.6763882926857043 1105 92-93 0.5785488686667151 1105 94-95 0.7458718245052296 1105 96-97 0.5911159048311276 1105 98-99 0.6136363243588789 1105 100-101 0.7568275958992636 1106 1 0.06387710446379202 1106 2 0.13585039087674033 1106 3 0.07649730094818352 1106 4 0.20417695204294262 1106 5 0.22545689693021842 1106 6 0.18030702521539865 1106 7 0.31547932365928233 1106 8 0.29184926357723384 1106 9 0.27032225376355257 1106 10-11 0.3449019089407699 1106 12-13 0.2091608658026871 1106 14-15 0.22147869052210467 1106 16-17 0.3422776862375585 1106 18-19 0.2794686628560612 1106 20-21 0.3113243391790732 1106 22-23 0.3474998662205664 1106 24-25 0.33402828453982636 1106 26-27 0.5814681181316885 1106 28-29 0.4903218698535454 1106 30-31 0.42311612132395027 1106 32-33 0.5808568336755755 1106 34-35 0.3637025200176325 1106 36-37 0.4005598494730762 1106 38-39 0.4117102528833101 1106 40-41 0.3037719722028527 1106 42-43 0.30678140805258636 1106 44-45 0.37180565540923993 1106 46-47 0.5098172334924485 1106 48-49 0.5564782910410031 1106 50-51 0.4515690310316103 1106 52-53 0.35759905969538863 1106 54-55 0.3030867191555302 1106 56-57 0.4863989284912975 1106 58-59 0.654009236855611 1106 60-61 0.8180063629669867 1106 62-63 0.3064959694691147 1106 64-65 0.47030818191648294 1106 66-67 0.5656817010417647 1106 68-69 -3.0502568998429176 1106 70-71 -3.2760422095914237 1106 72-73 0.858254251161167 1106 74-75 1.3717250802354108 1106 76-77 1.3617380874446532 1106 78-79 1.0260304377817455 1106 80-81 0.7903692619967728 1106 82-83 0.617180962320262 1106 84-85 0.6585591734312821 1106 86-87 0.6843819262875819 1106 88-89 0.6869794053837133 1106 90-91 0.7520637505258172 1106 92-93 0.6824367993285065 1106 94-95 0.7901577660615509 1106 96-97 0.8139342510708723 1106 98-99 0.6583459222356467 1106 100-101 0.699799375781712 1107 1 0.078989213006178 1107 2 0.05808511514847936 1107 3 0.05749076982286283 1107 4 -0.07303775528612988 1107 5 0.18847754239121883 1107 6 0.15821071161207811 1107 7 0.050639322566212286 1107 8 0.0344885623693898 1107 9 0.24344844931474086 1107 10-11 0.11357935606325498 1107 12-13 0.13163787706136532 1107 14-15 0.053224912580368766 1107 16-17 0.03653104744430635 1107 18-19 0.13394600203734797 1107 20-21 0.17659282581622193 1107 22-23 0.21907915517504506 1107 24-25 0.15951511298164434 1107 26-27 0.4141732747147415 1107 28-29 0.29545385870417107 1107 30-31 0.3040307179791455 1107 32-33 0.4082525474537917 1107 34-35 0.384771547727091 1107 36-37 0.40685654428643403 1107 38-39 0.44771127080974793 1107 40-41 0.2116793477288894 1107 42-43 0.07905449243800433 1107 44-45 0.3691595248960411 1107 46-47 0.5499903493037905 1107 48-49 0.5695640769621946 1107 50-51 0.4895802487175658 1107 52-53 0.0842222766161953 1107 54-55 0.08919052032512553 1107 56-57 0.5459723558291145 1107 58-59 0.5569952454909064 1107 60-61 0.43870630694687307 1107 62-63 0.21139144413708877 1107 64-65 0.06695220142787761 1107 66-67 0.33933837973226133 1107 68-69 -2.7501641592297013 1107 70-71 -2.7975858028034253 1107 72-73 1.021171771792968 1107 74-75 1.283430448601809 1107 76-77 0.815007202600146 1107 78-79 0.6702730390278511 1107 80-81 0.5650878634068413 1107 82-83 0.46638083115121987 1107 84-85 0.41340921715429957 1107 86-87 0.41208414523058323 1107 88-89 0.4514758733839308 1107 90-91 0.49782511167792265 1107 92-93 0.43261063247055276 1107 94-95 0.6282214172065466 1107 96-97 0.5772496511364551 1107 98-99 0.82300631738239 1107 100-101 1.1007163293806634 1108 1 -0.02966895431059413 1108 2 -0.015864988257369816 1108 3 0.03637677905521386 1108 4 0.01960830002072811 1108 5 -0.016572502548875434 1108 6 -0.17626977217022244 1108 7 -6.731899777463468E-4 1108 8 0.10187217625107792 1108 9 0.06030456247651017 1108 10-11 0.17153497444768107 1108 12-13 0.04132015495694219 1108 14-15 0.05287350722071693 1108 16-17 0.17350618640954707 1108 18-19 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2312 22-23 0.26724813972105466 2312 24-25 0.49366951302009454 2312 26-27 0.4487365477963223 2312 28-29 0.3878391553534115 2312 30-31 0.1525176954732146 2312 32-33 0.6038894290431429 2312 34-35 0.48224044969455804 2312 36-37 0.7352509813760051 2312 38-39 0.6952748541070406 2312 40-41 0.4352922247392854 2312 42-43 0.043751027197458825 2312 44-45 0.7100378285841131 2312 46-47 0.7924141389163637 2312 48-49 0.914838049864187 2312 50-51 1.0673498414611444 2312 52-53 0.8285243568091403 2312 54-55 0.8550998237379304 2312 56-57 0.9434056048362081 2312 58-59 1.0580480564384622 2312 60-61 1.016875859493485 2312 62-63 1.0614063432296028 2312 64-65 1.1555186990051318 2312 66-67 1.568621825136482 2312 68-69 4.194494013643464 2312 70-71 5.088427706872245 2312 72-73 3.2490696889784765 2312 74-75 2.5378010820972428 2312 76-77 2.528237640441219 2312 78-79 1.7970171722370942 2312 80-81 1.6610763373640118 2312 82-83 1.6477104768499231 2312 84-85 1.5259393539550565 2312 86-87 1.6022978445310656 2312 88-89 1.6330530799570084 2312 90-91 1.7260384876549928 2312 92-93 1.9465567510603385 2312 94-95 2.143670203214505 2312 96-97 2.1724219082397376 2312 98-99 2.520862377293348 2312 100-101 2.4979573320327866 2313 1 -0.3741491085066784 2313 2 -0.2583497115505722 2313 3 -0.2158746551197055 2313 4 -0.0727785185155696 2313 5 -0.07245369851911221 2313 6 -0.08409583628642281 2313 7 -2.800440044699098 2313 8 -3.1956069404235024 2313 9 -1.0715489737582544 2313 10-11 -0.20456688402288137 2313 12-13 -0.05564182697805364 2313 14-15 -0.5125251818374039 2313 16-17 -0.2967633502955067 2313 18-19 0.03349947379443563 2313 20-21 -2.6854948815116373 2313 22-23 -0.6178282194266771 2313 24-25 -0.07020046522026746 2313 26-27 -1.5290590055213755 2313 28-29 -1.7977182236823737 2313 30-31 -1.1791720788734565 2313 32-33 -1.0408969623041031 2313 34-35 -1.374931573588789 2313 36-37 -2.262400987747789 2313 38-39 -1.8964084947420616 2313 40-41 -0.934844617692292 2313 42-43 -0.1922653741043021 2313 44-45 -0.2054999138555118 2313 46-47 -0.14080061079453543 2313 48-49 -2.0830290419123934 2313 50-51 -0.7652048856477833 2313 52-53 -0.4288091493738335 2313 54-55 -0.517790948752797 2313 56-57 -0.5812623406673083 2313 58-59 -0.4098702514386865 2313 60-61 -0.03656270545367235 2313 62-63 0.07366160207037709 2313 64-65 0.026953557705247988 2313 66-67 0.7044152597935209 2313 68-69 1.5127533394609678 2313 70-71 4.008108204328508 2313 72-73 3.2109227592672234 2313 74-75 1.8453174268784203 2313 76-77 1.8183156638930313 2313 78-79 1.1260143906730136 2313 80-81 1.0607454179761397 2313 82-83 1.0189800011094654 2313 84-85 0.7869545262081807 2313 86-87 0.8790067936021089 2313 88-89 0.5344217704362144 2313 90-91 0.9761944280932617 2313 92-93 1.0736312207610759 2313 94-95 1.192154058212445 2313 96-97 1.4498622586736012 2313 98-99 1.5550205353424218 2313 100-101 1.6305103838779722 2314 1 -0.24255478789503115 2314 2 -0.18636919630856852 2314 3 -0.2160891349125258 2314 4 -0.06662446160033397 2314 5 -0.1600341658890656 2314 6 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2314 68-69 -1.4011166990880177 2314 70-71 1.4521301462612506 2314 72-73 1.0518594114113107 2314 74-75 0.7499058066943114 2314 76-77 0.7379344108283519 2314 78-79 -1.675000331303238 2314 80-81 -0.9020405100168389 2314 82-83 0.23368783103856572 2314 84-85 0.3007347513439207 2314 86-87 -0.04808211086161762 2314 88-89 -0.07522561594928234 2314 90-91 0.4020748018872631 2314 92-93 -0.08925597581589884 2314 94-95 0.44251308591014293 2314 96-97 0.5179718300222298 2314 98-99 0.24742043575962214 2314 100-101 0.43476794486190684 2315 1 -0.3500523414099135 2315 2 -0.3014523342053437 2315 3 -0.33923884255508696 2315 4 -0.08497364544209063 2315 5 -0.21582639018585326 2315 6 -0.034252197666688744 2315 7 -1.0237916257181183 2315 8 -2.722238402958528 2315 9 -0.6629051538753856 2315 10-11 -1.2850488316040583 2315 12-13 -0.5550837941664852 2315 14-15 -0.3872234208454657 2315 16-17 -0.20167438071603527 2315 18-19 -0.012079580420397917 2315 20-21 -0.705316380367627 2315 22-23 -0.28852223324539494 2315 24-25 -5.166687181413014 2315 26-27 -0.9417477919610349 2315 28-29 -0.8236623254451203 2315 30-31 -1.0419119947348108 2315 32-33 -0.11100153946338054 2315 34-35 -1.9238456674908804 2315 36-37 -3.358606838899668 2315 38-39 -0.8246216341568839 2315 40-41 0.01131879389771484 2315 42-43 -1.382484340212926 2315 44-45 -4.800345220581562 2315 46-47 -3.0863412235989607 2315 48-49 -2.240970584450224 2315 50-51 -0.7956453269090744 2315 52-53 0.021159084700798303 2315 54-55 -0.915249863411745 2315 56-57 -0.9235919482742432 2315 58-59 -0.22719669979200674 2315 60-61 -1.1751717067409366 2315 62-63 -0.19811590569618787 2315 64-65 0.24785984072888212 2315 66-67 0.5664159511065492 2315 68-69 3.325752808787957 2315 70-71 3.8813945706014046 2315 72-73 -2.240915299075393 2315 74-75 -1.6488858909549364 2315 76-77 0.07761779165416627 2315 78-79 0.0860202542885169 2315 80-81 -0.11873942938677828 2315 82-83 -0.8433914371571873 2315 84-85 -0.31244558788192833 2315 86-87 0.07529198562200179 2315 88-89 0.058698989815706426 2315 90-91 0.19276702600742723 2315 92-93 -1.0439570402174425 2315 94-95 0.19208495750788046 2315 96-97 0.15931934540217796 2315 98-99 -2.1209139637172427 2315 100-101 -3.9070391429683404 2316 1 -0.46795084383821717 2316 2 -0.4309418015495048 2316 3 -0.7975389238340149 2316 4 -0.3339668438180112 2316 5 -0.19103318175594097 2316 6 -0.3929975805582018 2316 7 -0.6081196423527402 2316 8 -0.9370301010897535 2316 9 -4.8444200659574115 2316 10-11 -1.4349516686885835 2316 12-13 -0.5895647216680757 2316 14-15 -0.3581892211052278 2316 16-17 -0.40951939239086954 2316 18-19 -0.4977178554150967 2316 20-21 -3.824120182008322 2316 22-23 -1.0273693979483554 2316 24-25 -0.41535962006798854 2316 26-27 -0.46176777454365237 2316 28-29 0.04882322799245031 2316 30-31 0.015993852387104823 2316 32-33 -1.0784809477562902 2316 34-35 -0.5360761223503872 2316 36-37 -0.3431232248704177 2316 38-39 -5.185893329850508 2316 40-41 -4.742905559927102 2316 42-43 -3.4120464569938918 2316 44-45 -2.159587445540822 2316 46-47 -2.3442668817036356 2316 48-49 -2.7706211961541953 2316 50-51 -1.1544498072491791 2316 52-53 -0.8126250649069888 2316 54-55 -0.060024504957802094 2316 56-57 -3.69405066547516 2316 58-59 -0.8202623388249002 2316 60-61 -0.4388536598376369 2316 62-63 -0.01986370077560551 2316 64-65 0.3501462217397311 2316 66-67 0.3847358741682143 2316 68-69 3.096928697864932 2316 70-71 3.8920910437954745 2316 72-73 2.661047258717179 2316 74-75 1.327566182031763 2316 76-77 -1.2829489421527747 2316 78-79 0.23249890435209508 2316 80-81 0.20704714967889615 2316 82-83 0.4144887634870358 2316 84-85 0.6561612644301107 2316 86-87 0.7512659550246461 2316 88-89 0.4968028411653549 2316 90-91 0.45089475393630707 2316 92-93 0.35694363349897174 2316 94-95 0.7226613559064177 2316 96-97 0.9303869395733706 2316 98-99 0.9922074353671384 2316 100-101 1.2657681216893408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 57.0 4 151.0 5 321.0 6 817.0 7 2187.0 8 4889.0 9 8179.0 10 12801.0 11 18812.0 12 24896.0 13 31927.0 14 39099.0 15 48263.0 16 57565.0 17 64518.0 18 60369.0 19 54676.0 20 54357.0 21 55864.0 22 59640.0 23 66158.0 24 78985.0 25 98228.0 26 122889.0 27 153015.0 28 186674.0 29 223962.0 30 261241.0 31 303416.0 32 351352.0 33 405766.0 34 465850.0 35 522796.0 36 539409.0 37 442211.0 38 183291.0 39 10674.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.09940308967057 20.512188988299712 15.419854452876072 22.968553469153647 2 15.81305599162742 21.349440338987378 39.03649522660847 23.801008442776737 3 18.930464138016152 25.15559896054012 30.642033761908817 25.271903139534906 4 13.074106495280452 17.821735345293167 37.02550555460421 32.078652604822175 5 13.446639496582472 38.12015983028002 34.075971224169145 14.357229448968361 6 31.440306899659443 36.976045396903885 17.267831364698562 14.315816338738108 7 28.018730609412763 33.38664332485265 20.651045197514815 17.943580868219776 8 26.386930445116164 35.22371453865357 19.434572469952066 18.954782546278203 9 26.21019197179841 16.787243884737165 20.46349983650096 36.53906430696347 10-11 24.95395273620903 27.068741812171833 27.07912132857012 20.898184123049017 12-13 25.787118269621878 24.415311485608097 27.737023759201808 22.06054648556822 14-15 22.74572709218953 25.702826539483027 26.382404313184406 25.16904205514304 16-17 21.900099784768372 28.955597722127784 26.9290965864517 22.21520590665214 18-19 21.688708330737118 27.31414044488753 29.616170477911396 21.380980746463955 20-21 22.763432841772808 26.333244937511463 29.566139349034557 21.33718287168117 22-23 22.254433571743178 26.44211836828529 29.45490141891443 21.848546641057098 24-25 21.977797491540063 26.581525944899703 29.654928736344516 21.785747827215722 26-27 22.05135981571027 26.763674799928577 29.47518083469745 21.709784549663706 28-29 21.85217187491744 26.97585082020117 29.483156767336354 21.688820537545038 30-31 22.035735559847964 26.698705215278213 29.430848027914514 21.83471119695931 32-33 21.96756757187871 26.70211028716391 29.340522550146165 21.989799590811216 34-35 22.358857329718308 26.771986861893858 29.233572257839075 21.63558355054876 36-37 22.253142807538147 26.570226369948784 29.286765534933334 21.889865287579735 38-39 22.089828235209584 26.841581609232108 29.291135930894907 21.777454224663394 40-41 22.275162486591856 26.62659946140944 29.26698225959602 21.831255792402683 42-43 22.087223140438148 26.720808424682374 29.085219140420186 22.10674929445929 44-45 22.187587022436087 26.911111224189348 28.862948075436623 22.038353677937938 46-47 22.297784098102994 27.018402542790156 28.449385499363395 22.234427859743455 48-49 22.413198447318962 27.070463372947845 28.331969328293948 22.18436885143925 50-51 22.239791459834038 27.256941170789652 28.252810654001653 22.250456715374657 52-53 22.48841084309371 27.329891366367388 27.949100279225064 22.23259751131384 54-55 22.233711471146737 27.16946820592236 28.13186541360598 22.464954909324923 56-57 22.3185531218541 27.434711134445926 27.979180160222416 22.267555583477556 58-59 22.457011664252526 27.112840729215716 28.01071913835263 22.419428468179127 60-61 22.455658780625814 27.169834460444747 28.22564828640013 22.14885847252931 62-63 22.252344988028934 27.146300235234065 28.220929600760087 22.380425175976914 64-65 22.167318292191112 27.13926009305043 28.095865970691058 22.5975556440674 66-67 21.98159901974332 27.303257723954545 28.13004975569467 22.585093500607467 68-69 22.047939395935256 26.843887023202427 28.630344281401026 22.477829299461284 70-71 21.475932236795135 26.834050135600823 29.09093651548625 22.599081112117798 72-73 22.13197406847928 26.98554525515379 28.25650951742516 22.625971158941766 74-75 22.1721684379276 27.240705094380914 27.77793848009434 22.809187987597152 76-77 21.515721711218024 27.289525325122995 28.310696900606324 22.884056063052654 78-79 22.074815050275138 27.26712726782523 27.7730194555664 22.885038226333236 80-81 21.98207736830325 27.38402057359001 27.7016669931762 22.932235064930534 82-83 22.1546916049749 27.325344907617875 27.576767766191796 22.943195721215428 84-85 22.04155231694274 27.28139164112491 27.453673906090863 23.22338213584149 86-87 21.95180091320031 27.236564620950286 27.687382376783308 23.12425208906609 88-89 22.021272703400324 27.16464347450367 27.492430413691064 23.32165340840494 90-91 21.933734062290352 27.250615913647362 27.61476541743198 23.200884606630304 92-93 22.007544205184036 27.085431369118208 27.640932921178656 23.266091504519096 94-95 21.745113994490335 26.99076814036673 27.66191671332548 23.60220115181746 96-97 21.76286357572779 26.82878910214268 27.761025352166612 23.647321969962917 98-99 21.609984102585784 26.80957721492937 27.54765435368197 24.032784328802876 100-101 21.522774200888133 26.721624017022833 27.73832968088769 24.017272101201343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1580.0 1 1159.0 2 757.0 3 808.5 4 814.0 5 881.0 6 1398.0 7 2714.5 8 4244.0 9 5216.0 10 5198.0 11 4906.5 12 4996.5 13 5241.0 14 5600.5 15 6140.0 16 6690.0 17 7136.5 18 7754.0 19 8587.0 20 9631.0 21 10952.5 22 12659.0 23 14974.0 24 17984.5 25 22356.0 26 28916.0 27 36459.0 28 43405.5 29 51035.5 30 60660.0 31 71083.0 32 81663.0 33 92089.0 34 102715.5 35 112472.0 36 122683.0 37 132797.0 38 139750.0 39 143835.0 40 147510.0 41 154917.5 42 162095.0 43 167568.0 44 173589.0 45 178696.0 46 182113.5 47 186755.0 48 196375.5 49 199340.0 50 192564.5 51 185501.5 52 178851.5 53 173411.5 54 166536.0 55 155354.0 56 141727.5 57 126464.0 58 111747.5 59 97374.0 60 82112.0 61 66090.5 62 51323.5 63 38924.5 64 29021.5 65 22029.5 66 16470.0 67 12158.0 68 8430.0 69 5728.5 70 4079.0 71 3053.5 72 2493.0 73 1926.5 74 1377.0 75 979.0 76 614.0 77 347.5 78 191.0 79 118.5 80 84.5 81 54.0 82 29.0 83 15.0 84 8.0 85 4.5 86 4.5 87 3.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019918968281186444 2 0.017067704553248846 3 4.585948653326208E-4 4 0.09734174489364587 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012671175518212197 12-13 0.0 14-15 0.0 16-17 5.084421333035579E-4 18-19 1.4954180391281115E-4 20-21 0.0 22-23 1.3957235031862374E-4 24-25 0.004207109416747087 26-27 0.0034594003971830313 28-29 0.0011265482561431772 30-31 1.4954180391281115E-4 32-33 6.181061228396194E-4 34-35 0.0027415997384015378 36-37 0.08523882823030235 38-39 0.09929575779810661 40-41 0.07789134093138624 42-43 0.08159997766842395 44-45 0.08056315449462846 46-47 0.07924718662019571 48-49 0.07438209326623227 50-51 0.07416276528716015 52-53 0.05734429707376598 54-55 0.048272094303055436 56-57 0.026967371972276943 58-59 0.022152125886284423 60-61 0.013109831476356443 62-63 0.002043737986808419 64-65 0.005931824888541509 66-67 0.15257251780544412 68-69 0.7043219575221521 70-71 0.8574727036360591 72-73 0.35129363629838173 74-75 0.05186109759696291 76-77 0.1913935701012099 78-79 0.01423637973249962 80-81 0.00304068334622716 82-83 0.0012760900600559885 84-85 7.975562875349928E-5 86-87 0.0020038601724316693 88-89 3.88808690173309E-4 90-91 0.004177201055964525 92-93 0.004237017777529649 94-95 0.07501016884266606 96-97 0.035571010424060676 98-99 0.01331919000183438 100-101 0.012830686775719196 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5015320.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.14948682136679 #Duplication Level Percentage of deduplicated Percentage of total 1 64.77862584030376 25.36049958641222 2 14.952860601461145 11.707936381172757 3 6.6449036461772675 7.804337031758073 4 3.847745273736207 6.025490115444479 5 2.3130007925994236 4.5276397023841035 6 1.5915887637184216 3.7385929998137883 7 1.1423195715567416 3.130485750871501 8 0.8700265501579194 2.7248874367717346 9 0.6315754365019337 2.225326880522829 >10 2.999035767370968 19.85816196430152 >50 0.14120147439749026 3.800196239743789 >100 0.08067969872698241 5.957249808020271 >500 0.004994789736408193 1.3589662105079452 >1k 0.0012873156743801416 0.8198504058366765 >5k 0.0 0.0 >10k+ 1.5447788092497384E-4 0.9603794864382311 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19584 0.3904835583771325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16833 0.3356316247019133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11223 0.2237743553751306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.993890718837482E-5 2 1.993890718837482E-5 0.0 0.0 0.0 1.993890718837482E-5 3 1.993890718837482E-5 0.0 0.0 0.0 1.993890718837482E-5 4 1.993890718837482E-5 0.0 0.0 0.0 3.987781437674964E-5 5 1.993890718837482E-5 0.0 0.0 0.0 3.987781437674964E-5 6 5.981672156512446E-5 0.0 0.0 0.0 3.987781437674964E-5 7 7.975562875349928E-5 0.0 0.0 0.0 3.987781437674964E-5 8 7.975562875349928E-5 0.0 0.0 0.0 3.987781437674964E-5 9 7.975562875349928E-5 0.0 0.0 0.0 3.987781437674964E-5 10-11 7.975562875349928E-5 0.0 0.0 0.0 5.981672156512446E-5 12-13 7.975562875349928E-5 0.0 0.0 9.96945359418741E-6 5.981672156512446E-5 14-15 1.3957235031862374E-4 0.0 0.0 3.987781437674964E-5 6.978617515931187E-5 16-17 1.6948071110118597E-4 0.0 0.0 3.987781437674964E-5 7.975562875349928E-5 18-19 2.1932797907212303E-4 0.0 0.0 3.987781437674964E-5 7.975562875349928E-5 20-21 2.5920579344887267E-4 0.0 0.0 5.981672156512446E-5 9.96945359418741E-5 22-23 2.5920579344887267E-4 0.0 0.0 1.5951125750699856E-4 1.4954180391281115E-4 24-25 4.3865595814424605E-4 0.0 0.0 2.691752470430601E-4 2.1932797907212303E-4 26-27 4.3865595814424605E-4 0.0 0.0 7.178006587814935E-4 2.1932797907212303E-4 28-29 7.078312051873061E-4 0.0 0.0 0.0019141350900839828 2.1932797907212303E-4 30-31 7.77617380346618E-4 0.0 0.0 0.005732435816657761 2.3926688626049785E-4 32-33 7.77617380346618E-4 0.0 0.0 0.013757845959978626 2.5920579344887267E-4 34-35 7.875868339408054E-4 0.0 0.0 0.026738074539610635 2.891141542314349E-4 36-37 8.374341019117425E-4 0.0 0.0 0.04827209430305544 3.090530614198097E-4 38-39 8.872813698826796E-4 0.0 0.0 0.08293588445004506 3.1902251501399713E-4 40-41 9.271591842594292E-4 0.0 0.0 0.1228934544555482 3.2899196860818454E-4 42-43 0.0010866704417664278 0.0 0.0 0.16228276560618266 3.3896142220237195E-4 44-45 0.0011763955241141143 0.0 0.0 0.21197052231961272 3.3896142220237195E-4 46-47 0.0012063038848966765 0.0 0.0 0.27145825191612893 3.5890032939074677E-4 48-49 0.0012561511528676136 0.0 0.0 0.3356017163411308 3.5890032939074677E-4 50-51 0.0012561511528676136 1.993890718837482E-5 0.0 0.4060359059840648 3.5890032939074677E-4 52-53 0.0012561511528676136 1.993890718837482E-5 0.0 0.4779356053053444 3.788392365791216E-4 54-55 0.0012561511528676136 1.993890718837482E-5 0.0 0.5585685459751322 3.987781437674964E-4 56-57 0.0012760900600559885 1.993890718837482E-5 0.0 0.6524110126572182 3.987781437674964E-4 58-59 0.0012760900600559885 1.993890718837482E-5 0.0 0.7460142124530438 3.987781437674964E-4 60-61 0.0012760900600559885 1.993890718837482E-5 0.0 0.8426979734094733 4.1871705095587123E-4 62-63 0.0012860595136501758 5.981672156512446E-5 0.0 0.9391624063868307 4.3865595814424605E-4 64-65 0.0013159678744327382 7.975562875349928E-5 0.0 1.0450878508250718 4.3865595814424605E-4 66-67 0.0014156624103746122 7.975562875349928E-5 0.0 1.1594873308183726 4.4862541173843346E-4 68-69 0.001435601317562987 7.975562875349928E-5 0.0 1.2900472950878508 4.585948653326209E-4 70-71 0.0014555402247513618 7.975562875349928E-5 0.0 1.454164440155364 4.785337725209957E-4 72-73 0.0014854485855339242 7.975562875349928E-5 0.0 1.6352296563329958 5.981672156512446E-4 74-75 0.0015552347606932358 7.975562875349928E-5 0.0 1.829992901749041 5.981672156512446E-4 76-77 0.0015751736678816107 7.975562875349928E-5 0.0 2.055372339152836 5.981672156512446E-4 78-79 0.001605082028664173 7.975562875349928E-5 0.0 2.3126041807900592 5.981672156512446E-4 80-81 0.0018543183685188582 7.975562875349928E-5 0.0 2.5974314699759935 5.981672156512446E-4 82-83 0.001973951811649107 7.975562875349928E-5 1.993890718837482E-5 2.893494333362577 6.181061228396194E-4 84-85 0.0020337685332142315 7.975562875349928E-5 1.993890718837482E-5 3.191551087467998 6.181061228396194E-4 86-87 0.0020736463475909813 7.975562875349928E-5 1.993890718837482E-5 3.502797428678529 6.181061228396194E-4 88-89 0.0020736463475909813 7.975562875349928E-5 1.993890718837482E-5 3.8507512980228578 6.181061228396194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15885 0.0 37.869316 1 GTATCAA 25095 0.0 33.27949 1 ATCAACG 31435 0.0 26.253876 3 TATCAAC 31510 0.0 26.242332 2 TCAACGC 31555 0.0 26.116735 4 CAACGCA 32220 0.0 25.549137 5 AACGCAG 32710 0.0 25.224392 6 GTACATG 22975 0.0 23.372498 1 TACATGG 23385 0.0 22.389317 2 ACGCAGA 37180 0.0 22.089737 7 CGCAGAG 37335 0.0 21.934523 8 ACATGGG 23865 0.0 20.919994 3 GAGTACT 18855 0.0 20.880644 12-13 AGTACTT 19560 0.0 20.370401 12-13 GCAGAGT 39205 0.0 20.283533 9 AGAGTAC 32545 0.0 19.878466 10-11 GTACTTT 20210 0.0 19.422037 14-15 CAGAGTA 38405 0.0 17.376175 10-11 CATGGGA 14585 0.0 17.153057 4 ACCGTCG 470 0.0 17.151539 8 >>END_MODULE