FastQCFastQC Report
Fri 27 May 2016
SRR939485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045271
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.7306611202134093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118740.5805587621395893No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA87250.4265938352423713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80730.39471541913027663No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA69320.3389281909341109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66540.3253358601378497No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31340.15323152775353488No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29660.14501745734428348No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29450.14399069854312704No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.1385146516036261No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27220.13308749794037072No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26940.1317184862054955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24700.12076639232649367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.11695271677934123No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG23150.11318793450843434No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC23100.11294346812720663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG169450.024.8122141
TACATGG168800.024.5498052
GAGTACT131200.023.82181712-13
ACATGGG173350.023.4431843
TAGCCCT15100.023.1502234
GTGTAGC13750.022.258211
GTACTTT146100.021.83234214-15
CATGGGA126550.021.7956374
AGTACTT139950.021.73346712-13
CATGGGG43200.021.1718524
GTAGCCC12750.020.6726953
AACGCGG3800.020.4626546
ATGGGAT46650.020.4207945
ATGGGAG41600.020.2261855
ACTTTTT172500.020.09757416-17
CAACGCG4100.020.0504045
TACTTTT163300.018.80774714-15
TGTAGCC14650.018.0127812
GTATCAA395750.017.9263381
ACGCGGA3551.1623342E-917.8057447
Produced by FastQC (version 0.11.5)