FastQCFastQC Report
Fri 27 May 2016
SRR939485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045271
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205701.00573469237084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183800.8986584173930985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130810.6395729465679609No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA117850.576207260553736No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA101730.497391299245919No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99230.485167980184533No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT67580.33042076086738625No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG48530.23727906961962497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.23331871424373593No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA46620.22794045385672607No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45510.2225133001934707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.2108278071707857No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT42110.20588958626998577No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC38020.18589223628555823No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.17572243482648509No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31440.1537204605159903No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28510.1393947305760459No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG26980.13191405931047767No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT22510.11005876482871953No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.10252920028690575No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA20690.1011601885520305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.10111129527578495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG182000.021.9543321
GAGTACT152800.021.92250612-13
TACATGG180800.021.7811952
CATTCCG2658.203642E-1021.4545279
GTACTTT161300.020.87006814-15
AGTACTT158300.020.59214812-13
ACATGGG185700.020.0791973
CGCATAG2400.020.07336866-67
GTGTAGC12650.019.8586371
TATCACG1708.10183E-519.5131872
CATGGGG45250.019.3703164
TACCTGG12800.019.2518482
ACTTTTT181500.018.91280216-17
ACCGTAT1559.79471E-418.3401588
TACTTTT177950.018.33158914-15
GTATAGC8850.018.2095721
CATGGGA137150.018.205324
GCAACGC3050.018.13702668-69
TAGCCCT15150.017.8256664
ATGGGAT51550.017.554495
Produced by FastQC (version 0.11.5)