##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2045271 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.985037190670575 31.0 31.0 31.0 30.0 34.0 2 31.206607828498033 31.0 31.0 34.0 30.0 34.0 3 31.182552336585225 31.0 31.0 34.0 28.0 34.0 4 35.11580812518243 35.0 35.0 37.0 32.0 37.0 5 34.75890432123665 35.0 35.0 37.0 32.0 37.0 6 34.74360072577179 35.0 35.0 37.0 32.0 37.0 7 34.617710318094765 35.0 35.0 37.0 30.0 37.0 8 34.660041627735396 35.0 35.0 37.0 30.0 37.0 9 35.95631923593499 37.0 35.0 39.0 30.0 39.0 10-11 35.81718143952561 37.0 35.0 39.0 30.0 39.0 12-13 35.776801705006335 37.0 35.0 39.0 30.0 39.0 14-15 36.51354050392344 38.0 35.0 40.0 30.0 41.0 16-17 36.56591082550919 38.0 35.0 40.0 30.5 41.0 18-19 36.633189684887725 38.0 35.0 40.0 30.0 41.0 20-21 36.56886226812975 38.0 35.0 40.0 30.0 41.0 22-23 36.429113061300924 38.0 35.0 40.0 30.0 41.0 24-25 36.18864590560371 38.0 34.5 40.0 29.5 41.0 26-27 35.75655475484667 38.0 34.0 40.0 28.0 41.0 28-29 35.47185214086544 38.0 34.0 40.0 27.0 41.0 30-31 35.22409499768001 37.5 34.0 40.0 27.0 41.0 32-33 34.93356479410308 37.0 33.0 40.0 27.0 41.0 34-35 34.81648471033912 37.0 33.0 40.0 26.0 41.0 36-37 34.711083519005555 37.0 33.0 40.0 25.0 41.0 38-39 34.620016369468885 37.0 33.0 40.0 25.0 41.0 40-41 34.35566802638868 37.0 33.0 40.0 24.5 41.0 42-43 34.012939850024765 37.0 33.0 40.0 24.0 41.0 44-45 33.75703855381512 37.0 33.0 40.0 23.0 41.0 46-47 33.306208566004216 37.0 32.0 40.0 21.0 41.0 48-49 32.90479672375935 37.0 31.5 40.0 18.5 41.0 50-51 32.614566969364944 36.5 31.0 40.0 16.5 41.0 52-53 32.20170872221823 36.0 31.0 39.0 14.0 41.0 54-55 32.00550171590953 36.0 30.5 39.0 12.5 40.0 56-57 31.64980606481977 35.5 30.0 39.0 9.0 40.0 58-59 31.252063662957134 35.0 29.5 39.0 8.0 40.0 60-61 30.963585265717846 35.0 29.0 38.5 7.5 40.0 62-63 30.560611283296932 35.0 28.0 38.0 6.5 40.0 64-65 30.103199771570615 34.0 28.0 38.0 2.0 40.0 66-67 28.500316095030925 33.5 25.0 37.0 2.0 39.5 68-69 25.03003733979507 29.5 13.5 35.0 2.0 39.0 70-71 24.443321447377876 29.5 11.0 35.0 2.0 38.0 72-73 26.15759891965417 31.0 21.0 35.0 2.0 38.0 74-75 26.71379171757679 31.5 22.0 35.0 2.0 37.5 76-77 26.681622875403797 31.5 24.0 34.5 2.0 36.5 78-79 27.46961576240997 32.5 26.0 35.0 2.0 37.0 80-81 27.34563170357376 33.0 26.0 35.0 2.0 36.5 82-83 26.95136488025303 32.5 25.0 35.0 2.0 36.0 84-85 26.622421185261025 32.0 25.0 35.0 2.0 36.0 86-87 26.158855965786444 32.0 24.0 34.5 2.0 35.0 88-89 25.621963788661745 32.0 22.0 34.0 2.0 35.0 90-91 25.151667187380056 31.0 20.0 34.0 2.0 35.0 92-93 24.642111241004248 31.0 18.5 34.0 2.0 35.0 94-95 23.359056330432495 30.5 4.5 34.0 2.0 35.0 96-97 22.561200691742073 30.0 2.0 34.0 2.0 35.0 98-99 22.220148576887855 30.0 2.0 34.0 2.0 35.0 100-101 21.006506717202758 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41014462399580154 1101 2 0.30182407783857457 1101 3 0.3642724220175637 1101 4 0.2606028041886077 1101 5 0.41852684651613714 1101 6 0.6087999403638875 1101 7 0.5557407472365767 1101 8 0.5649718308870462 1101 9 0.6273454464177917 1101 10-11 0.7575733627732077 1101 12-13 0.6769946110931997 1101 14-15 0.9071721028776167 1101 16-17 0.8337748583193161 1101 18-19 0.9674635628100887 1101 20-21 0.8263461980277853 1101 22-23 0.9917379748802304 1101 24-25 0.8853291746716607 1101 26-27 0.8906528155750806 1101 28-29 0.861861769375615 1101 30-31 0.9417096869333719 1101 32-33 0.9543741922655045 1101 34-35 0.9563584076046183 1101 36-37 1.0339986254139006 1101 38-39 0.9685911111605492 1101 40-41 1.2447346021542742 1101 42-43 1.251573365871856 1101 44-45 1.02632262887645 1101 46-47 0.9827338907312111 1101 48-49 1.0906065533455163 1101 50-51 0.9082802180439913 1101 52-53 1.0086396929457493 1101 54-55 1.0397040080288171 1101 56-57 0.860528233047166 1101 58-59 0.8087119251620116 1101 60-61 0.6705608936355958 1101 62-63 0.6964511311897255 1101 64-65 0.475313465671082 1101 66-67 -2.297345523904543 1101 68-69 -7.774970938965964 1101 70-71 -11.112184028298667 1101 72-73 -11.083884079797691 1101 74-75 -9.462519442015502 1101 76-77 -4.34558196802573 1101 78-79 -1.394778393014569 1101 80-81 -0.6682272385790355 1101 82-83 -0.44955302394429353 1101 84-85 -0.49536284020905796 1101 86-87 -0.4855246861107112 1101 88-89 -0.45068222361276256 1101 90-91 -0.7241684590822892 1101 92-93 -1.249939121645415 1101 94-95 -4.820810904335946 1101 96-97 -1.9605645704987644 1101 98-99 -1.2159121068404346 1101 100-101 -0.9460868817277266 1102 1 0.39219317081938243 1102 2 0.2727665041419378 1102 3 0.3979742225858942 1102 4 0.2735157454571535 1102 5 0.39317244721810596 1102 6 0.43283035858249264 1102 7 0.35963774654455705 1102 8 0.4843405409880148 1102 9 0.6488163295099412 1102 10-11 0.6663412496818779 1102 12-13 0.6487441095175441 1102 14-15 0.6298294776878635 1102 16-17 0.7459143714219607 1102 18-19 0.7486339853926225 1102 20-21 0.8596629645842526 1102 22-23 0.8396737842555382 1102 24-25 0.7721667109473742 1102 26-27 0.8836576588584748 1102 28-29 0.6653259908795874 1102 30-31 0.3569196894385911 1102 32-33 0.560981507762861 1102 34-35 0.7790773070432166 1102 36-37 0.908964439157387 1102 38-39 0.8046005425266713 1102 40-41 0.776655633709403 1102 42-43 0.8562403056032366 1102 44-45 0.5653533384491496 1102 46-47 0.6111467212001287 1102 48-49 0.6673839941166335 1102 50-51 0.5466541686907291 1102 52-53 0.6847463387540174 1102 54-55 0.5266045576034593 1102 56-57 0.3849306275099984 1102 58-59 0.3888975708898883 1102 60-61 0.2766295230506195 1102 62-63 0.25948644117985253 1102 64-65 0.31729033938630735 1102 66-67 -2.830871980345403 1102 68-69 -7.837122297633069 1102 70-71 -11.151110637264463 1102 72-73 -9.407992759555302 1102 74-75 -7.839811622539347 1102 76-77 -4.351699234435241 1102 78-79 -1.6669140738538033 1102 80-81 -1.0426535155712955 1102 82-83 -0.7080675816538893 1102 84-85 -0.6378486053283119 1102 86-87 -0.6683048294569289 1102 88-89 -0.6860858085867143 1102 90-91 -0.7455154635155488 1102 92-93 -1.1001806690802063 1102 94-95 -4.420630838996068 1102 96-97 -1.4366456749732386 1102 98-99 -0.7023245851324305 1102 100-101 -0.445560636678092 1103 1 0.3751241825207714 1103 2 0.1949551677210799 1103 3 0.3881989594369948 1103 4 0.25314012392639285 1103 5 0.4182548004338926 1103 6 0.42005107353720916 1103 7 0.5843076980308695 1103 8 0.6076784190489875 1103 9 0.6553383123894747 1103 10-11 0.7653072442540463 1103 12-13 0.6262820953681754 1103 14-15 0.7519869214473829 1103 16-17 0.6344451308638668 1103 18-19 0.5598984005920471 1103 20-21 0.7685372504152426 1103 22-23 0.7987871788919847 1103 24-25 0.8686999354628924 1103 26-27 0.9365189824204379 1103 28-29 1.0396620486875179 1103 30-31 0.962547614212582 1103 32-33 0.9980164668749012 1103 34-35 0.9808759271188592 1103 36-37 1.0206905450538528 1103 38-39 1.0308087683030678 1103 40-41 1.2226781703343406 1103 42-43 1.2053553133331647 1103 44-45 0.9615847435869611 1103 46-47 1.1100723675941992 1103 48-49 0.9973184033180189 1103 50-51 0.9069717355288631 1103 52-53 1.0936448001462722 1103 54-55 1.0834314026468697 1103 56-57 1.2257175712063635 1103 58-59 1.085248457486916 1103 60-61 0.8441450923204812 1103 62-63 0.9100884126551883 1103 64-65 0.6974678171833482 1103 66-67 -2.6968589247086854 1103 68-69 -7.510047690600565 1103 70-71 -11.15879346390443 1103 72-73 -8.928559284655996 1103 74-75 -7.1280408960595665 1103 76-77 -3.4537143716711505 1103 78-79 -0.6424436124298722 1103 80-81 0.005286413994461725 1103 82-83 0.33662657040288835 1103 84-85 0.3235157216303115 1103 86-87 0.43755745237426424 1103 88-89 0.45689856858205147 1103 90-91 0.388505720111592 1103 92-93 0.3877816728981145 1103 94-95 1.3670259322243439 1103 96-97 1.5351204998319083 1103 98-99 1.2305142468211585 1103 100-101 1.0303422684324168 1104 1 0.3396660674448242 1104 2 0.14513633159651107 1104 3 0.2858425994373235 1104 4 0.029699895709100588 1104 5 0.35776246096066444 1104 6 0.5178967809370505 1104 7 0.4398331962315325 1104 8 0.3750185070897132 1104 9 0.5391889054757399 1104 10-11 0.6307709336030882 1104 12-13 0.6014544469736407 1104 14-15 0.7813182546003716 1104 16-17 0.6497928261478734 1104 18-19 0.6428048511736577 1104 20-21 0.7181948015059163 1104 22-23 0.5592340224598473 1104 24-25 0.6616460207656658 1104 26-27 0.8769009021017169 1104 28-29 1.0027210130203699 1104 30-31 0.9434901582124482 1104 32-33 0.8133330118092772 1104 34-35 0.8927702372615727 1104 36-37 0.8902478412035038 1104 38-39 0.9876819289978869 1104 40-41 0.9572480905730529 1104 42-43 0.9958837626195773 1104 44-45 0.9805159586965217 1104 46-47 0.9399793674056625 1104 48-49 0.8502592422461177 1104 50-51 0.9091522600023794 1104 52-53 1.0792531336845457 1104 54-55 0.8511541071530182 1104 56-57 1.036892759133142 1104 58-59 1.2308925319526445 1104 60-61 0.9316226517725603 1104 62-63 0.9894070397445205 1104 64-65 0.7856481039305088 1104 66-67 -2.352501236720421 1104 68-69 -6.779834925515189 1104 70-71 -8.31518912252939 1104 72-73 -7.581358922328246 1104 74-75 -6.283791619485449 1104 76-77 -3.1178849116378693 1104 78-79 -0.35797759500206894 1104 80-81 0.40106308238259203 1104 82-83 0.5113857700028532 1104 84-85 0.5466833842884213 1104 86-87 0.5836878411798097 1104 88-89 0.6287294303810675 1104 90-91 0.5268390944322299 1104 92-93 0.4701354097104513 1104 94-95 1.4268458750060695 1104 96-97 1.4552118605283653 1104 98-99 1.2750192841605923 1104 100-101 1.116654968757853 1105 1 0.4484993589406514 1105 2 0.3671705506895542 1105 3 0.47929781936313276 1105 4 0.38449088749699456 1105 5 0.41364182550875483 1105 6 0.5620377374619139 1105 7 0.5694067326162084 1105 8 0.6117631907384222 1105 9 0.7943022452639994 1105 10-11 0.8863066329468339 1105 12-13 0.9032634209188402 1105 14-15 1.1730451871941767 1105 16-17 1.0949418672721478 1105 18-19 1.0572707655621727 1105 20-21 1.1228073862268602 1105 22-23 1.1106080855986136 1105 24-25 1.1422401930007098 1105 26-27 1.172814573402384 1105 28-29 1.213849390402622 1105 30-31 1.1104733953195236 1105 32-33 1.0161937008928845 1105 34-35 1.2110443605666532 1105 36-37 1.1019253856209872 1105 38-39 0.9405888385611405 1105 40-41 1.1800873827621885 1105 42-43 1.4955790461383884 1105 44-45 1.1869218781055366 1105 46-47 1.046007653542297 1105 48-49 0.9473567156593816 1105 50-51 0.7623046162507094 1105 52-53 1.1185569775549453 1105 54-55 1.08835073544871 1105 56-57 1.121488987382623 1105 58-59 0.9871780191204884 1105 60-61 1.014345556012497 1105 62-63 1.159716557577255 1105 64-65 1.2465590259095443 1105 66-67 2.0518416953314897 1105 68-69 -4.597568149672561 1105 70-71 -5.112946831587408 1105 72-73 -1.3982037968356558 1105 74-75 1.1532245098681422 1105 76-77 1.1582555587750498 1105 78-79 1.1208734475486608 1105 80-81 1.257052021343668 1105 82-83 1.2517450146964642 1105 84-85 1.1275692236881945 1105 86-87 1.301692311815863 1105 88-89 1.4137612667286952 1105 90-91 1.3791234221035573 1105 92-93 1.4731118492193964 1105 94-95 2.3911789055155737 1105 96-97 2.486750464590191 1105 98-99 2.086651405003238 1105 100-101 1.765841328934929 1106 1 0.23705917934897514 1106 2 0.2110854036970018 1106 3 0.2774846019626054 1106 4 0.11358538101413984 1106 5 0.2933592113839083 1106 6 0.29717768419271806 1106 7 0.39297660552635705 1106 8 0.3660133550246272 1106 9 0.5074034246366281 1106 10-11 0.47119272087029884 1106 12-13 0.38980241639416846 1106 14-15 0.5443878135689815 1106 16-17 0.4443719929667864 1106 18-19 0.5002707781190807 1106 20-21 0.6155411225904075 1106 22-23 0.6678013098914874 1106 24-25 0.7178774211611056 1106 26-27 0.7451060833983831 1106 28-29 0.9287769021677406 1106 30-31 0.7087486343542082 1106 32-33 0.9377939531471924 1106 34-35 0.959531602051328 1106 36-37 0.8032760902727603 1106 38-39 0.7312763571349024 1106 40-41 0.7885790610207337 1106 42-43 0.9282328647636362 1106 44-45 0.9019463570985238 1106 46-47 0.8104854187476676 1106 48-49 0.7570505085011021 1106 50-51 0.5265312433119647 1106 52-53 0.6178789045658277 1106 54-55 0.6928834999076479 1106 56-57 0.652086682213497 1106 58-59 0.6350851314922501 1106 60-61 0.5050018218394321 1106 62-63 0.6027291270413713 1106 64-65 0.5309506507314801 1106 66-67 1.2658588313241879 1106 68-69 -5.548711316552463 1106 70-71 -6.139766990167196 1106 72-73 -2.3167771368474064 1106 74-75 0.4556966858545941 1106 76-77 0.6092515307982609 1106 78-79 0.48634711501664896 1106 80-81 0.65035807363342 1106 82-83 0.7427528739251628 1106 84-85 0.5987846067366469 1106 86-87 0.5864697127314003 1106 88-89 0.5594172046808303 1106 90-91 0.5863856396254654 1106 92-93 0.667101415824721 1106 94-95 1.5842573987425155 1106 96-97 1.642246134550021 1106 98-99 1.3599608616100554 1106 100-101 1.1374420695134084 1107 1 0.007874779589460701 1107 2 -0.06221621545855527 1107 3 -0.11467175931502283 1107 4 -0.14148261403176576 1107 5 -0.01060292960152509 1107 6 0.051924859358727815 1107 7 0.05900156368365117 1107 8 -0.0320227446883834 1107 9 0.15263541835016525 1107 10-11 0.0030172073367751295 1107 12-13 0.17744760260067238 1107 14-15 0.02790740668150704 1107 16-17 -0.10146987820407816 1107 18-19 0.03666810802318565 1107 20-21 0.008767811586935181 1107 22-23 0.004029618971685522 1107 24-25 0.19858501926073302 1107 26-27 0.28017568718428265 1107 28-29 0.2565565419131062 1107 30-31 0.20472564999713683 1107 32-33 0.1374790113557367 1107 34-35 0.4117731646741092 1107 36-37 0.25493386590096634 1107 38-39 0.338373581749039 1107 40-41 0.36265761909471195 1107 42-43 0.6056746175477272 1107 44-45 0.4170797536127182 1107 46-47 0.28247478043882523 1107 48-49 0.41270486865055744 1107 50-51 0.33214481777754656 1107 52-53 0.32312979066188063 1107 54-55 0.4069496624336928 1107 56-57 0.572378359149635 1107 58-59 0.556575938471294 1107 60-61 0.39271795760471306 1107 62-63 0.5819287376437927 1107 64-65 0.42748897225879645 1107 66-67 1.1884299690849218 1107 68-69 -5.376761657739404 1107 70-71 -6.008250948657576 1107 72-73 -2.210537700648903 1107 74-75 0.33056043279566794 1107 76-77 0.24277952854602347 1107 78-79 0.38045654852360755 1107 80-81 0.24087795446290983 1107 82-83 0.46224440896218155 1107 84-85 0.2738877053142552 1107 86-87 0.38142425035786687 1107 88-89 0.21608228666984886 1107 90-91 0.38870748456148974 1107 92-93 0.44009644626187594 1107 94-95 1.2434893491438217 1107 96-97 1.3664324060166457 1107 98-99 1.009567390619452 1107 100-101 0.9500326255908895 1108 1 -0.037869480636832265 1108 2 -0.018594140146493743 1108 3 -0.047349186798332 1108 4 -0.1797837598354377 1108 5 -0.0675950361962947 1108 6 0.11273091677387725 1108 7 0.001447720334866176 1108 8 0.005615666469324765 1108 9 0.2582792133305887 1108 10-11 0.10948926697674466 1108 12-13 -0.011937682846415498 1108 14-15 0.154854996355553 1108 16-17 -0.13820914180038102 1108 18-19 -0.006495277065589278 1108 20-21 0.18061162746347748 1108 22-23 0.10186994396774196 1108 24-25 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0.3640100161129034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 29.0 4 93.0 5 221.0 6 540.0 7 1235.0 8 2508.0 9 4117.0 10 6854.0 11 9871.0 12 13096.0 13 15853.0 14 19818.0 15 25210.0 16 30208.0 17 33831.0 18 29459.0 19 23122.0 20 21843.0 21 21991.0 22 23200.0 23 26282.0 24 31731.0 25 38705.0 26 48154.0 27 60042.0 28 72859.0 29 86545.0 30 101838.0 31 118435.0 32 137484.0 33 158759.0 34 185261.0 35 210969.0 36 217508.0 37 183634.0 38 78961.0 39 4998.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68045947668212 20.507922441791486 15.69953652285035 20.11208155867605 2 15.420141417233655 22.610619447388125 39.36605291971963 22.603186215658592 3 20.276677271618286 27.7536815414681 30.73005973291559 21.239581453998028 4 12.443681057424664 18.135640704825914 37.614770854326885 31.80590738342254 5 12.17174855728024 40.494233276894136 32.989205820848014 14.344812344977603 6 28.742059120771767 37.64513357887537 17.669296635995916 15.94351066435695 7 26.096688409506612 36.19554572474748 20.085064522011997 17.622701343733908 8 26.05424904572548 34.19204594403382 19.333281506460512 20.42042350378018 9 26.893844385413963 17.61644300437448 20.680877986340196 34.80883462387136 10-11 24.49597143850375 27.963604823028344 26.01630786335894 21.524115875108972 12-13 27.268733581026673 25.637556098922833 27.03712613145153 20.05658418859897 14-15 23.872484379820573 26.300255565154934 27.89803893958307 21.929221115441425 16-17 20.080014081304867 29.857549021764907 28.666603105221082 21.395833791709144 18-19 19.76774915285231 28.094280124317596 32.36156735218339 19.776403370646705 20-21 21.766974099513973 26.508034022883525 31.775748000081162 19.949243877521337 22-23 20.723537369864435 26.729367404123956 31.53474527336475 21.01234995264686 24-25 20.81061629485775 26.79664455223782 32.38240800363375 20.010331149270684 26-27 20.999597606762556 26.39809647952366 32.737721261275446 19.864584652438342 28-29 20.020181925855507 26.866950397061174 33.209219624049894 19.903648053033425 30-31 20.188316127007038 27.543959498879804 32.44680903080319 19.820915343309974 32-33 19.918316952716246 27.339372157840693 32.090337880788226 20.651973008654835 34-35 20.617091528316163 26.843090298533916 32.093086936803125 20.44673123634679 36-37 21.07733764272958 26.559117735296116 32.44522184108253 19.91832278089177 38-39 21.089674239545193 26.482398373754947 32.60188686124867 19.82604052545119 40-41 20.504420180625218 27.013433208049403 32.72164719858617 19.760499412739215 42-43 19.952516963121607 27.68414726299592 31.866148930922016 20.497186842960453 44-45 20.298911321294433 27.95032271783301 31.060511704195342 20.690254256677214 46-47 20.613569621814285 27.76440222451533 30.160794569038696 21.46123358463169 48-49 20.766611093859318 27.770987177143645 30.22333612543255 21.23906560356449 50-51 21.30715985857299 28.17102889886559 30.05837816039667 20.463433082164748 52-53 20.792653285502805 28.410243261935758 30.050664381317908 20.74643907124353 54-55 20.199210506424144 28.216310574257868 30.362796686935244 21.22168223238274 56-57 20.948422890139597 28.932136514437783 28.977110153869027 21.14233044155359 58-59 20.777087534270724 28.174918414097693 29.559170837346304 21.488823214285276 60-61 20.87831433200665 27.76722533362444 30.07679663816977 21.277663696199138 62-63 21.715457215513688 27.642759599244883 29.773146316885214 20.868636868356216 64-65 21.272334552868884 28.124502640968817 30.022161890593846 20.581000915568453 66-67 20.405783112655122 28.126492824378307 30.347152703690593 21.12057135927597 68-69 20.567726113893166 28.485784423163835 30.1541045509855 20.792384911957505 70-71 20.359622289804552 28.23156070707088 30.00209882842953 21.406718174695037 72-73 20.71621127700721 28.037909194335242 29.06783398339971 22.17804554525783 74-75 20.407459772436546 28.70274141128514 29.267892008348483 21.621906807929832 76-77 20.88093128950128 28.11978975519933 29.371116499453898 21.628162455845487 78-79 20.433344490117445 28.128396085557945 29.848513038645912 21.589746385678698 80-81 20.25943133882657 28.925958657714183 29.332340785994816 21.482269217464435 82-83 20.372899239269515 28.72944465550042 28.844661660973042 22.05299444425702 84-85 20.413578445105806 28.19423440707857 28.784889630762866 22.607297517052753 86-87 20.635809252334962 28.292118268148297 29.047642912235194 22.02442956728154 88-89 21.048729095837125 27.64332982195632 29.09353912333245 22.21440195887411 90-91 20.166224408819343 27.99861332973464 29.58123144729553 22.25393081415049 92-93 20.103303481847572 28.607358759575913 29.165313929541625 22.12402382903489 94-95 19.667351609304866 28.658148616464054 29.085725303783533 22.588774470447547 96-97 19.298631186849477 28.19139617863297 28.954341615011835 23.555631019505714 98-99 19.961417621242315 28.120832773016787 28.5327937994352 23.3849558063057 100-101 20.351672671227785 27.876272790927832 28.963425963956986 22.808628573887397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7809.0 1 5115.5 2 2591.0 3 2849.5 4 2622.0 5 2603.0 6 4146.5 7 7486.0 8 10437.5 9 11500.0 10 10230.0 11 8028.0 12 6898.5 13 6302.0 14 5984.0 15 5760.0 16 5535.5 17 5614.0 18 5865.0 19 6061.0 20 6429.0 21 6940.0 22 7799.5 23 9072.0 24 10552.5 25 12341.0 26 15211.0 27 18595.5 28 21059.5 29 23618.5 30 26789.5 31 29962.0 32 34133.0 33 38269.0 34 42495.5 35 47262.0 36 53079.5 37 57553.5 38 58046.5 39 57875.0 40 58263.5 41 58556.5 42 58846.5 43 60371.5 44 63034.0 45 65522.5 46 69613.0 47 80949.0 48 107129.0 49 105571.5 50 78418.5 51 66488.5 52 59300.5 53 54712.5 54 51399.0 55 47352.5 56 42761.5 57 38532.5 58 35174.5 59 32136.5 60 28209.0 61 23318.0 62 17872.5 63 12324.0 64 8203.0 65 5960.0 66 4596.0 67 3556.0 68 2665.0 69 1993.0 70 1458.5 71 1102.0 72 899.0 73 738.0 74 548.0 75 362.5 76 257.5 77 167.5 78 106.5 79 74.0 80 45.0 81 27.5 82 18.0 83 8.0 84 4.5 85 2.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024935570885227434 2 0.019557310498217597 3 0.0 4 0.0 5 9.778655249108798E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.933596574732639E-4 18-19 0.0012467785442613717 20-21 2.4446638122771996E-5 22-23 0.0 24-25 0.0 26-27 3.9114620996435193E-4 28-29 0.018188298763342364 30-31 0.004547074690835591 32-33 0.008360750237988022 34-35 0.009460848953512761 36-37 0.09700426007115927 38-39 0.09957115707405033 40-41 0.11301680804157493 42-43 0.09851995163477113 44-45 0.09399732358205833 46-47 0.09531744204068801 48-49 0.09805546551043846 50-51 0.09561080169816127 52-53 0.07468447946506844 54-55 0.06368349230982105 56-57 0.036181024421702554 58-59 0.016648160561607728 60-61 0.009509742229758306 62-63 0.0015645848398574077 64-65 0.004571521328958363 66-67 0.41285482461737344 68-69 0.7917777155203395 70-71 0.877609861969392 72-73 0.27416904654688795 74-75 0.1458730896785805 76-77 0.015719188312942393 78-79 0.008678556533584058 80-81 0.0013934583729980038 82-83 0.0 84-85 0.0 86-87 1.7112646685940396E-4 88-89 7.089525055603878E-4 90-91 0.004107035204625695 92-93 0.0017112646685940394 94-95 0.21843071162696778 96-97 0.22302667959404893 98-99 0.014472409768681022 100-101 0.007774030923041494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2045271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.54371699526793 #Duplication Level Percentage of deduplicated Percentage of total 1 68.65152780607016 29.206911702742154 2 14.83971539730328 12.626733043063815 3 6.35495061009632 8.110896608245293 4 3.2508628362933183 5.532151539907878 5 1.9419282508682023 4.130842296502623 6 1.1883665621907333 3.033451842508921 7 0.8233163123656512 2.4518855333610317 8 0.5583392025319125 1.9003060015905011 9 0.40378417797211336 1.546063181533124 >10 1.80456937441156 13.309145609894605 >50 0.10653484624679205 3.1381773985785872 >100 0.06803928124964548 5.501036103218195 >500 0.0036235596291570586 1.176437391472675 >1k 0.0036235596291570586 3.827039302234185 >5k 2.3377804059077794E-4 0.8295283479374261 >10k+ 5.844451014769449E-4 3.6793940972089336 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20570 1.00573469237084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18380 0.8986584173930985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13081 0.6395729465679609 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11785 0.576207260553736 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10173 0.497391299245919 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9923 0.485167980184533 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6758 0.33042076086738625 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4853 0.23727906961962497 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4772 0.23331871424373593 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4662 0.22794045385672607 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4551 0.2225133001934707 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.2108278071707857 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4211 0.20588958626998577 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3802 0.18589223628555823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3594 0.17572243482648509 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3144 0.1537204605159903 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2851 0.1393947305760459 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2698 0.13191405931047767 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2251 0.11005876482871953 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2097 0.10252920028690575 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2069 0.1011601885520305 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2068 0.10111129527578495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.889327624554399E-5 0.0 5 0.0 0.0 0.0 4.889327624554399E-5 0.0 6 0.0 0.0 0.0 4.889327624554399E-5 0.0 7 0.0 0.0 0.0 4.889327624554399E-5 0.0 8 0.0 0.0 0.0 4.889327624554399E-5 4.889327624554399E-5 9 0.0 0.0 0.0 4.889327624554399E-5 4.889327624554399E-5 10-11 0.0 0.0 0.0 9.778655249108798E-5 4.889327624554399E-5 12-13 0.0 0.0 0.0 9.778655249108798E-5 4.889327624554399E-5 14-15 0.0 0.0 0.0 9.778655249108798E-5 4.889327624554399E-5 16-17 0.0 0.0 0.0 1.7112646685940396E-4 9.778655249108798E-5 18-19 0.0 0.0 0.0 2.9335965747326396E-4 1.4667982873663195E-4 20-21 0.0 0.0 0.0 6.356125911920719E-4 1.4667982873663195E-4 22-23 0.0 0.0 0.0 0.0011978852680158277 1.7112646685940396E-4 24-25 0.0 0.0 0.0 0.0021268575166811634 1.9557310498217597E-4 26-27 0.0 0.0 0.0 0.003495869251556395 1.9557310498217597E-4 28-29 0.0 0.0 0.0 0.006209446083184087 1.9557310498217597E-4 30-31 0.0 0.0 0.0 0.014056816920593896 1.9557310498217597E-4 32-33 0.0 0.0 0.0 0.024837784332736347 2.4446638122771995E-4 34-35 0.0 0.0 0.0 0.04500626078402324 2.4446638122771995E-4 36-37 0.0 0.0 0.0 0.07818034871662484 2.4446638122771995E-4 38-39 0.0 0.0 0.0 0.12394445528245401 2.4446638122771995E-4 40-41 0.0 0.0 0.0 0.17332666429045346 2.4446638122771995E-4 42-43 0.0 0.0 0.0 0.22503130392011622 2.6891301935049193E-4 44-45 0.0 0.0 0.0 0.28492556732090757 2.933596574732639E-4 46-47 0.0 0.0 0.0 0.3595611535097305 2.933596574732639E-4 48-49 0.0 0.0 0.0 0.4406506521629652 2.933596574732639E-4 50-51 0.0 0.0 0.0 0.53117655313159 2.933596574732639E-4 52-53 0.0 0.0 0.0 0.6177176520862027 4.1559284808712386E-4 54-55 0.0 0.0 0.0 0.7093436517703522 5.378260387009839E-4 56-57 0.0 0.0 0.0 0.8105527335986282 5.622726768237558E-4 58-59 0.0 0.0 0.0 0.9199269925599102 6.111659530692998E-4 60-61 0.0 0.0 0.0 1.041842376878174 6.356125911920719E-4 62-63 9.778655249108798E-5 0.0 0.0 1.1585506272762875 6.356125911920719E-4 64-65 9.778655249108798E-5 0.0 0.0 1.2699295105636368 6.356125911920719E-4 66-67 9.778655249108798E-5 0.0 0.0 1.3860754882849267 6.600592293148439E-4 68-69 9.778655249108798E-5 0.0 0.0 1.5278170961207587 6.845058674376158E-4 70-71 9.778655249108798E-5 0.0 0.0 1.7204810511663247 7.333991436831598E-4 72-73 9.778655249108798E-5 0.0 0.0 1.937347177953435 7.333991436831598E-4 74-75 9.778655249108798E-5 0.0 0.0 2.161522849539254 7.333991436831598E-4 76-77 9.778655249108798E-5 0.0 0.0 2.3939370381724476 7.578457818059319E-4 78-79 9.778655249108798E-5 0.0 0.0 2.666272586860128 7.822924199287039E-4 80-81 9.778655249108798E-5 0.0 0.0 2.976744891019332 8.556323342970198E-4 82-83 9.778655249108798E-5 0.0 0.0 3.2936711076429477 9.289722486653357E-4 84-85 9.778655249108798E-5 0.0 0.0 3.6260720461982787 9.289722486653357E-4 86-87 9.778655249108798E-5 0.0 0.0 3.967787153878386 9.289722486653357E-4 88-89 9.778655249108798E-5 0.0 0.0 4.31759409877713 9.289722486653357E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18200 0.0 21.954332 1 GAGTACT 15280 0.0 21.922506 12-13 TACATGG 18080 0.0 21.781195 2 CATTCCG 265 8.203642E-10 21.454527 9 GTACTTT 16130 0.0 20.870068 14-15 AGTACTT 15830 0.0 20.592148 12-13 ACATGGG 18570 0.0 20.079197 3 CGCATAG 240 0.0 20.073368 66-67 GTGTAGC 1265 0.0 19.858637 1 TATCACG 170 8.10183E-5 19.513187 2 CATGGGG 4525 0.0 19.370316 4 TACCTGG 1280 0.0 19.251848 2 ACTTTTT 18150 0.0 18.912802 16-17 ACCGTAT 155 9.79471E-4 18.340158 8 TACTTTT 17795 0.0 18.331589 14-15 GTATAGC 885 0.0 18.209572 1 CATGGGA 13715 0.0 18.20532 4 GCAACGC 305 0.0 18.137026 68-69 TAGCCCT 1515 0.0 17.825666 4 ATGGGAT 5155 0.0 17.55449 5 >>END_MODULE