FastQCFastQC Report
Fri 27 May 2016
SRR939484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034702
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146680.7208918062694193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.5339848292280638No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA83520.4104777996974496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80650.3963725400574629No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67640.3324319728392659No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA64330.31616423436945557No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28600.14056112393854236No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG27900.13712081670927734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.13363136223387995No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26910.13225523934217395No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT26460.13004361326621786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.12655415879082046No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25260.12414594373033497No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.11697044579501077No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG21750.10689526033787748No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC21620.10625634613815685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT126900.024.57317712-13
GTACATG165500.024.2844091
TACATGG166450.024.1056182
ACATGGG177550.022.7798843
GTACTTT140500.022.76218214-15
ATGGGAG43450.022.2617725
AGTACTT132850.021.93312612-13
CATGGGA134750.021.6243554
ATGGGAT49150.020.965675
ACTTTTT170750.020.78309416-17
GTATCAA377200.019.476181
CATTCCG4250.019.4240079
TACCTGG10400.019.313842
CATGGGG42600.018.5835744
TACTTTT159450.018.30125814-15
GTGTAGC12450.018.2496931
ATCGACG2453.307121E-617.6876183
CATGGGT15300.017.4553014
GTATTAG5550.017.2851471
AGTACAT125000.017.2057132
Produced by FastQC (version 0.11.5)