FastQCFastQC Report
Fri 27 May 2016
SRR939484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034702
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201360.9896289481211499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179270.8810626814147724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129000.6339994751074113No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA111210.5465665242379474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97490.4791365025443529No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA96230.4729439495316759No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT64530.31714717929210273No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG47010.23104120406821244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44790.2201305154268291No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43890.21570726327491693No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA43760.21506834907519626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.2110874221384753No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT42050.20666416998656312No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC37140.18253287213557565No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.17481675449279552No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30570.15024313142661677No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28250.13884097032390985No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG25600.1258169500988351No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20850.10247200818596532No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG20650.10148906326331816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG181750.023.2190441
TACATGG181300.023.0905552
GAGTACT153750.022.0598612-13
ACATGGG183350.021.6653353
GTACTTT162900.020.70437414-15
CATTCCG4400.020.4736189
CATGGGA136700.020.1745224
AGTACTT160200.020.0768512-13
ATGGGAG43950.019.3102465
ACTTTTT183650.018.93291316-17
TTTAGGC6250.018.224353
GTATAGG6800.018.15021
TACCTGG11250.018.1417642
CATGGGG42100.017.9271244
GTGTTCG2654.0879786E-717.8915429
CATGGGT13600.017.8002834
TACTTTT181150.017.70262714-15
CGCATAG2750.017.46023466-67
GTCGGGA6050.017.2596052
ATGGGAT50150.017.1120195
Produced by FastQC (version 0.11.5)