FastQCFastQC Report
Fri 27 May 2016
SRR939483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184593
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162530.7439829753185148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129130.5910940848020662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.38144404930346293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43910.20099853840051674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.14419161830144103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.11301876367817713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86850.055.6804581
GTATCAA163850.045.5400541
TATCAAC214400.034.6025542
ATCAACG215500.034.544043
TCAACGC220500.033.7525444
CAACGCA222650.033.1296165
AACGCAG226400.032.551126
GTATCGA1905.456968E-1231.6499461
ACGCAGA251000.028.6601097
CGCAGAG253050.028.3658758
GTGGTAT40500.028.3351671
TGGTATC41800.027.691552
GTACATG157900.027.6427541
ATCGACG1954.8567017E-1027.022623
GCAGAGT247600.026.5888619
TACATGG162800.026.2499712
AACGCGG3200.025.791086
GAGTACT143800.025.10408612-13
CAACGCG3500.024.8430295
ACATGGG167550.024.6451933
Produced by FastQC (version 0.11.5)