FastQCFastQC Report
Fri 27 May 2016
SRR939483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184593
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231911.061570736517054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205330.93990047574079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143560.6571475785192025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71940.32930619113033865No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.24068556477110384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.19903020837290972No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.15417059379023917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99250.054.0599141
GTATCAA176600.041.4396441
ATCAACG239600.030.4328253
TATCAAC240000.030.4292452
TCAACGC244450.029.8096394
CAACGCA249000.029.2458955
AACGCAG254650.028.6156146
GTGGTAT46400.028.2957521
TGGTATC46750.027.5731932
ACGCAGA290950.024.9965727
CGCAGAG292550.024.8112758
GTACATG182400.024.5045241
TACATGG186300.023.7847462
TACACCG1601.9024046E-623.6897475
GCAGAGT298900.023.4757549
GAGTACT170850.022.78205712-13
ACATGGG187250.022.4435843
AGTACTT175650.021.70092612-13
GTACTTT182100.021.27052314-15
CATGGGA131000.020.543174
Produced by FastQC (version 0.11.5)