##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2184593 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.000108029275935 31.0 31.0 31.0 30.0 34.0 2 31.254072955465848 31.0 31.0 34.0 30.0 34.0 3 31.220231411526083 31.0 31.0 34.0 28.0 34.0 4 35.17800569717105 35.0 35.0 37.0 33.0 37.0 5 34.81366460480282 35.0 35.0 37.0 32.0 37.0 6 34.790061581264794 35.0 35.0 37.0 32.0 37.0 7 34.65335511008229 35.0 35.0 37.0 30.0 37.0 8 34.68072908775227 35.0 35.0 37.0 31.0 37.0 9 35.99781378041585 37.0 35.0 39.0 30.0 39.0 10-11 35.86418362596602 37.0 35.0 39.0 30.0 39.0 12-13 35.81485315571368 37.0 35.0 39.0 30.0 39.0 14-15 36.60639098449917 38.0 35.5 40.0 30.0 41.0 16-17 36.636636893004784 38.0 35.5 40.0 30.5 41.0 18-19 36.68114815894768 38.5 35.0 40.0 30.5 41.0 20-21 36.62446300065962 38.0 35.0 40.0 30.0 41.0 22-23 36.48363974433681 38.0 35.0 40.0 30.0 41.0 24-25 36.26217423565854 38.0 34.5 40.0 29.5 41.0 26-27 35.88289512050986 38.0 34.0 40.0 28.5 41.0 28-29 35.59869389858889 38.0 34.0 40.0 27.0 41.0 30-31 35.32122413648675 38.0 34.0 40.0 27.0 41.0 32-33 35.02127032357973 37.0 33.0 40.0 27.0 41.0 34-35 34.87677727613336 37.0 33.0 40.0 26.5 41.0 36-37 34.78620731642003 37.5 33.0 40.0 25.0 41.0 38-39 34.73237005703122 37.0 33.0 40.0 25.0 41.0 40-41 34.47733742623912 37.0 33.0 40.0 24.5 41.0 42-43 34.09972132108818 37.0 33.0 40.0 24.0 41.0 44-45 33.807423396486215 37.0 33.0 40.0 23.0 41.0 46-47 33.33762032561671 37.0 32.0 40.0 21.0 41.0 48-49 32.925350168200666 37.0 31.5 40.0 18.5 41.0 50-51 32.62824494081964 36.5 31.0 40.0 17.0 41.0 52-53 32.24609069057715 36.0 31.0 39.0 14.0 41.0 54-55 32.02060292237501 36.0 31.0 39.0 12.5 40.5 56-57 31.645040288969156 35.0 30.0 39.0 9.0 40.0 58-59 31.23251585077861 35.0 29.5 39.0 8.0 40.0 60-61 30.951422759296584 35.0 29.0 38.5 7.5 40.0 62-63 30.562496767132366 35.0 28.0 38.0 6.5 40.0 64-65 30.151094048181974 34.0 28.0 38.0 2.0 40.0 66-67 28.53919906362421 33.5 25.0 37.0 2.0 39.5 68-69 25.019555816575444 29.5 13.5 35.0 2.0 38.0 70-71 24.45055234544833 29.5 11.0 35.0 2.0 38.0 72-73 26.2067375936845 31.0 21.0 35.0 2.0 38.0 74-75 26.77974295440844 31.5 22.5 35.0 2.0 37.5 76-77 26.773301937706474 31.5 24.5 34.5 2.0 36.5 78-79 27.575476072659757 32.5 26.0 35.0 2.0 37.0 80-81 27.434759701234967 33.0 26.0 35.0 2.0 36.5 82-83 27.03405600036254 33.0 25.0 35.0 2.0 36.0 84-85 26.707560630286743 32.0 25.0 35.0 2.0 36.0 86-87 26.266906467245846 32.0 24.0 35.0 2.0 35.0 88-89 25.786847252554594 32.0 23.0 34.0 2.0 35.0 90-91 25.353612091588687 32.0 20.5 34.0 2.0 35.0 92-93 24.822616386667907 31.0 19.0 34.0 2.0 35.0 94-95 23.50417949705048 31.0 7.0 34.0 2.0 35.0 96-97 22.73739296061097 30.0 2.0 34.0 2.0 35.0 98-99 22.423894977233743 30.0 2.0 34.0 2.0 35.0 100-101 21.233684489513607 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44059077467356644 1101 2 0.30503136948809484 1101 3 0.37695974556947576 1101 4 0.3265772250953063 1101 5 0.3577257129758351 1101 6 0.6135535271701258 1101 7 0.6349777153679099 1101 8 0.5440301843200643 1101 9 0.5881232373475029 1101 10-11 0.7891123067775894 1101 12-13 0.6673053202703301 1101 14-15 0.8078240675041286 1101 16-17 0.7761677566558873 1101 18-19 0.8934033172857667 1101 20-21 0.7859540719606954 1101 22-23 0.8304998251002544 1101 24-25 0.7304783534411143 1101 26-27 0.8883595158370596 1101 28-29 0.650150615790146 1101 30-31 0.7937413868827079 1101 32-33 0.8385925169732289 1101 34-35 0.9347318384361998 1101 36-37 0.9955581166551966 1101 38-39 0.9475147108483668 1101 40-41 1.0797438620166204 1101 42-43 1.166598806932349 1101 44-45 0.9047131010275393 1101 46-47 0.8841714999902806 1101 48-49 0.640539530109649 1101 50-51 0.8153610457205716 1101 52-53 0.8686296504170343 1101 54-55 0.8825401023450468 1101 56-57 0.7649223167605541 1101 58-59 0.7042940503131732 1101 60-61 0.38913213509961864 1101 62-63 0.3259254774384708 1101 64-65 0.18866586553183495 1101 66-67 -2.6711722652821557 1101 68-69 -7.8623131617950115 1101 70-71 -11.114269203654942 1101 72-73 -11.008982567977005 1101 74-75 -9.465362825134086 1101 76-77 -4.458249431566571 1101 78-79 -1.4277361118342107 1101 80-81 -0.9476330290870401 1101 82-83 -0.8384774539913487 1101 84-85 -0.9313848540248237 1101 86-87 -0.8720328326604481 1101 88-89 -0.8919188020954962 1101 90-91 -0.9494594603446913 1101 92-93 -1.3683326291981395 1101 94-95 -4.894218423074516 1101 96-97 -2.0042392104690663 1101 98-99 -1.3374993389204128 1101 100-101 -1.0786182334772363 1102 1 0.3698014393519742 1102 2 0.32057380899056653 1102 3 0.41289636484225767 1102 4 0.24427992929534526 1102 5 0.4991322815902137 1102 6 0.5601116474370968 1102 7 0.3793020120639454 1102 8 0.5080917779026421 1102 9 0.6866935573786392 1102 10-11 0.7207398165094858 1102 12-13 0.760635196074503 1102 14-15 0.6637098854033923 1102 16-17 0.7992125399985568 1102 18-19 0.7682266133595093 1102 20-21 0.9570830005067208 1102 22-23 0.9111069288572295 1102 24-25 0.8753921934108888 1102 26-27 0.9163103686446021 1102 28-29 0.895718407726477 1102 30-31 0.7942638586181232 1102 32-33 0.7569237599430565 1102 34-35 0.9338279006774428 1102 36-37 1.0381750825911453 1102 38-39 0.9941820613957191 1102 40-41 1.1094345894881386 1102 42-43 1.1256406686509663 1102 44-45 1.0009838126935549 1102 46-47 0.9795359506095096 1102 48-49 0.9502008519032756 1102 50-51 0.74966149833282 1102 52-53 0.9061819378013283 1102 54-55 0.8016773475473684 1102 56-57 0.9599591301529884 1102 58-59 1.0497534876699994 1102 60-61 0.9476778318519088 1102 62-63 0.8934404957084503 1102 64-65 0.8664637030153344 1102 66-67 -2.0798851851583855 1102 68-69 -6.734166402192059 1102 70-71 -10.330145017118504 1102 72-73 -8.754916813921707 1102 74-75 -7.198274952304438 1102 76-77 -3.7078461623786225 1102 78-79 -1.0242292336506331 1102 80-81 -0.17794548630726936 1102 82-83 0.034184916141317245 1102 84-85 -0.1350892295625279 1102 86-87 0.040217382601124285 1102 88-89 0.05075209986567941 1102 90-91 0.0237257746952082 1102 92-93 -0.3330838818971742 1102 94-95 -3.9648145680605573 1102 96-97 -0.6820754715623174 1102 98-99 -0.021745998479012485 1102 100-101 0.03479226757021081 1103 1 0.31148815841146416 1103 2 0.1441429676896142 1103 3 0.2835536739178188 1103 4 0.01962820086846051 1103 5 0.37269218533872817 1103 6 0.3129631236449484 1103 7 0.38272547881272345 1103 8 0.3932200238386869 1103 9 0.5539273997054224 1103 10-11 0.5194862464339423 1103 12-13 0.3946897812537813 1103 14-15 0.519071529082602 1103 16-17 0.5592997441212404 1103 18-19 0.3688539606139045 1103 20-21 0.4387088963772854 1103 22-23 0.5731300610438623 1103 24-25 0.6239071630771349 1103 26-27 0.6612841520510884 1103 28-29 0.6530804181439223 1103 30-31 0.6415067483077621 1103 32-33 0.6644225187417447 1103 34-35 0.6356647056263327 1103 36-37 0.5871505509175918 1103 38-39 0.58465655492504 1103 40-41 0.606568149668675 1103 42-43 0.6875602664844394 1103 44-45 0.46542430336162255 1103 46-47 0.5669792316019127 1103 48-49 0.48621186442946396 1103 50-51 0.4370854909703823 1103 52-53 0.38402117498581845 1103 54-55 0.39260126839418064 1103 56-57 0.4525743715861026 1103 58-59 0.46470992020659807 1103 60-61 0.2576675562619748 1103 62-63 0.28101403796724966 1103 64-65 0.21058110076542746 1103 66-67 -3.1381293567019206 1103 68-69 -7.793000641703259 1103 70-71 -11.18776011151035 1103 72-73 -9.092619147034188 1103 74-75 -7.502584818427685 1103 76-77 -4.081472667173031 1103 78-79 -1.2468042203736722 1103 80-81 -0.6049326625786087 1103 82-83 -0.4211290688673941 1103 84-85 -0.4565170348937251 1103 86-87 -0.486860786267993 1103 88-89 -0.29475111860823944 1103 90-91 -0.30632245484139986 1103 92-93 -0.3727635741138471 1103 94-95 0.42277808956336216 1103 96-97 0.5539973847922006 1103 98-99 0.18749589132999134 1103 100-101 -0.059969862902104154 1104 1 0.3947338429680407 1104 2 0.19520764002657032 1104 3 0.3300271548288194 1104 4 0.18271347194811938 1104 5 0.4306136035590953 1104 6 0.4880662624440859 1104 7 0.6002915909109845 1104 8 0.4307454778286228 1104 9 0.6143393404217363 1104 10-11 0.7453492071020875 1104 12-13 0.5833532184854775 1104 14-15 0.6676909114958391 1104 16-17 0.690883601042934 1104 18-19 0.7207148115666087 1104 20-21 0.7549468777414603 1104 22-23 0.7737715954692632 1104 24-25 0.9590306821371186 1104 26-27 1.002630478227431 1104 28-29 0.9989824324442438 1104 30-31 0.9679939184726933 1104 32-33 0.8200433059250543 1104 34-35 1.059416414649462 1104 36-37 1.1494860707763976 1104 38-39 1.0892468075617572 1104 40-41 1.1870932230222522 1104 42-43 1.3203811062399424 1104 44-45 0.8880094387435733 1104 46-47 0.836322130344108 1104 48-49 0.8050342472130865 1104 50-51 0.8890233226403481 1104 52-53 1.068700732082121 1104 54-55 0.8784341707379184 1104 56-57 0.8753045072326131 1104 58-59 0.9638269462588589 1104 60-61 0.709529346003908 1104 62-63 0.9121010193502066 1104 64-65 0.6207843742381485 1104 66-67 -2.4536630754509083 1104 68-69 -7.269718668675527 1104 70-71 -8.442394231207683 1104 72-73 -7.795387807177125 1104 74-75 -6.62401609649535 1104 76-77 -3.322402512306951 1104 78-79 -0.38621390970271463 1104 80-81 0.282005450728132 1104 82-83 0.5078704719793272 1104 84-85 0.576338113341091 1104 86-87 0.6379891354711269 1104 88-89 0.6408335166550714 1104 90-91 0.5391901326153494 1104 92-93 0.7082857703552321 1104 94-95 1.7776272773399846 1104 96-97 1.7505165249978099 1104 98-99 1.4552693051602326 1104 100-101 1.3935002434883188 1105 1 0.5092561998177167 1105 2 0.29904148127150876 1105 3 0.5376133896902218 1105 4 0.39623909159678306 1105 5 0.4780612166541971 1105 6 0.5356509033089836 1105 7 0.6354364173785996 1105 8 0.5254858486565297 1105 9 0.7747883720339033 1105 10-11 0.9492779890161032 1105 12-13 0.9370418172856461 1105 14-15 1.0704108078580958 1105 16-17 1.0824526878577174 1105 18-19 1.0350309975524397 1105 20-21 1.0403809656363805 1105 22-23 1.1707038163608274 1105 24-25 1.16966438663394 1105 26-27 1.2124198190050066 1105 28-29 1.1546783132916048 1105 30-31 1.1962557911249618 1105 32-33 1.1754669489481344 1105 34-35 1.418119635571081 1105 36-37 1.2314737704366934 1105 38-39 1.0452872852853048 1105 40-41 1.118152349805797 1105 42-43 1.3090955410668101 1105 44-45 1.240795821539031 1105 46-47 1.3236238158555906 1105 48-49 1.1062263538542538 1105 50-51 1.199398911562632 1105 52-53 1.3744336314009686 1105 54-55 1.4123889180160631 1105 56-57 1.376555060412315 1105 58-59 1.3874196326113797 1105 60-61 1.4423958061501558 1105 62-63 1.4597723512531324 1105 64-65 1.4671730982509352 1105 66-67 1.9685899197594132 1105 68-69 -4.88271764026813 1105 70-71 -5.155054163938235 1105 72-73 -1.5759519910544064 1105 74-75 1.2642022768312167 1105 76-77 1.3117279241474726 1105 78-79 1.2462220315627128 1105 80-81 1.3522606719405879 1105 82-83 1.4705120527764244 1105 84-85 1.2554014236663456 1105 86-87 1.3645308202275537 1105 88-89 1.4130437256433979 1105 90-91 1.334396887245454 1105 92-93 1.6207657492697365 1105 94-95 2.353221338164932 1105 96-97 2.4601562538226496 1105 98-99 2.0440760589513935 1105 100-101 1.8250073322284805 1106 1 0.12946566139184412 1106 2 0.12623955514598606 1106 3 0.12236227906650399 1106 4 0.0428759233611018 1106 5 0.2500107991427569 1106 6 0.2527116115382171 1106 7 0.270188809633396 1106 8 0.2923578415057264 1106 9 0.33030406792208566 1106 10-11 0.4500676328877731 1106 12-13 0.3468558091554499 1106 14-15 0.33271594483825595 1106 16-17 0.2171882698160985 1106 18-19 0.2992973944563886 1106 20-21 0.37161192643547736 1106 22-23 0.36028241831449037 1106 24-25 0.37776652705113634 1106 26-27 0.43339116545870127 1106 28-29 0.6253751502451053 1106 30-31 0.49508556566279793 1106 32-33 0.5281078905218877 1106 34-35 0.5212270408675082 1106 36-37 0.459843583580124 1106 38-39 0.4411183669777188 1106 40-41 0.6248659949434341 1106 42-43 0.7646460369089425 1106 44-45 0.6643465539574933 1106 46-47 0.4674612491996797 1106 48-49 0.4926275633506805 1106 50-51 0.34056077940307716 1106 52-53 0.6935446325166055 1106 54-55 0.6096599468167625 1106 56-57 0.5914447760665951 1106 58-59 0.7119606235070641 1106 60-61 0.6034009527412145 1106 62-63 0.5762635688853912 1106 64-65 0.5298483961980374 1106 66-67 1.3514780413399272 1106 68-69 -4.969654332515788 1106 70-71 -5.8649932032905205 1106 72-73 -2.127125349744592 1106 74-75 0.4985189743666325 1106 76-77 0.6335841082770735 1106 78-79 0.46739871006640854 1106 80-81 0.5997809331884376 1106 82-83 0.5715738826308758 1106 84-85 0.528504491730267 1106 86-87 0.7047486006981067 1106 88-89 0.5689021931569229 1106 90-91 0.4996193059496292 1106 92-93 0.6143644588022497 1106 94-95 1.4603466683420372 1106 96-97 1.6395973669142272 1106 98-99 1.3964072862156875 1106 100-101 1.5436842052387512 1107 1 0.06795134360505628 1107 2 -0.022689827360238723 1107 3 0.02492308084293171 1107 4 -0.05418903727296254 1107 5 0.10052488064276588 1107 6 0.0772444894059987 1107 7 0.17800664164261093 1107 8 -0.030948904306491443 1107 9 0.16081966390114388 1107 10-11 0.1226967720256269 1107 12-13 0.22817385115840239 1107 14-15 0.24451883749323855 1107 16-17 0.060689822507704605 1107 18-19 0.38803938121451864 1107 20-21 0.2796495682513651 1107 22-23 0.23538452294956613 1107 24-25 0.3897253023704508 1107 26-27 0.5559749228111102 1107 28-29 0.407055633027376 1107 30-31 0.4112148026107576 1107 32-33 0.30383957321328126 1107 34-35 0.4861771927711587 1107 36-37 0.385149529382808 1107 38-39 0.34797133209631426 1107 40-41 0.3375786092217439 1107 42-43 0.47620386091105615 1107 44-45 0.3579584147123569 1107 46-47 0.246639511096582 1107 48-49 0.3962227332843469 1107 50-51 0.3417529934382273 1107 52-53 0.38273505582051826 1107 54-55 0.37368421564239895 1107 56-57 0.397596453246976 1107 58-59 0.34713427676007313 1107 60-61 0.3833091970463194 1107 62-63 0.38572694198081336 1107 64-65 0.37136499972635306 1107 66-67 1.1550219749047805 1107 68-69 -5.27400809168417 1107 70-71 -5.709738329086939 1107 72-73 -2.2127165539868194 1107 74-75 0.25741934338067196 1107 76-77 0.3525244917908452 1107 78-79 0.2971551383638875 1107 80-81 0.22412982989746055 1107 82-83 0.5110696557619114 1107 84-85 0.4552808319215238 1107 86-87 0.4976130029381345 1107 88-89 0.43082044213868187 1107 90-91 0.5855832578424831 1107 92-93 0.6863426173499754 1107 94-95 1.3309403932102732 1107 96-97 1.5306735445093338 1107 98-99 1.1525994722049369 1107 100-101 0.8812997982028747 1108 1 4.99303530471451E-4 1108 2 -0.011946382673492906 1108 3 0.10150725959834261 1108 4 0.08180065963927063 1108 5 0.10959591716710548 1108 6 0.2115954896904384 1108 7 0.25152043703467086 1108 8 0.0373364427642926 1108 9 0.2393543709795125 1108 10-11 0.31021586644953203 1108 12-13 0.19165662140375872 1108 14-15 0.19457809605017928 1108 16-17 0.06965235003001169 1108 18-19 0.35513112926293644 1108 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1.519657023591975 2316 96-97 1.3743446703474227 2316 98-99 0.6241129204634852 2316 100-101 0.40926946031908784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 21.0 4 86.0 5 211.0 6 519.0 7 1231.0 8 2549.0 9 4350.0 10 7005.0 11 10475.0 12 13262.0 13 16963.0 14 20822.0 15 26870.0 16 32504.0 17 36638.0 18 31422.0 19 24429.0 20 22759.0 21 22836.0 22 24256.0 23 27542.0 24 33165.0 25 40790.0 26 50758.0 27 62455.0 28 76642.0 29 91623.0 30 107656.0 31 124732.0 32 144575.0 33 168918.0 34 196380.0 35 227447.0 36 237246.0 37 202261.0 38 87746.0 39 5446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60482902100976 20.813661104111112 15.999428604916929 20.5820812699622 2 16.024397513548706 22.291895683323133 38.91409820814163 22.769608594986533 3 18.769628942324726 27.59205032699455 30.729659941233905 22.90866078944682 4 12.79144444754698 18.504911441170048 36.590065060173686 32.11357905110929 5 12.695196810385474 39.3582319794561 32.89514279567333 15.051428414485098 6 29.680952012571677 37.582561145256804 17.222933516677934 15.513553325493582 7 27.15558458715193 35.44220822826037 20.183896954718797 17.218310229868905 8 27.272265360183795 34.219051329011855 19.19222482174025 19.316458489064097 9 27.683325910135203 16.536489863329233 20.303278459649004 35.47690576688656 10-11 24.980030605243172 28.158631836685373 25.61136101781888 21.249976540252575 12-13 26.455889037454572 25.912080648431996 27.151922577798242 20.480107736315187 14-15 23.509390536360776 26.298537073038318 26.992190307302096 23.19988208329881 16-17 20.735348408518146 30.00498722067616 27.50606694594187 21.753597424863823 18-19 20.632020417790173 26.869507918164963 32.17500230595054 20.32346935809432 20-21 22.048276738110197 25.832529407658406 32.27167080359381 19.84752305063759 22-23 21.548315864785796 26.053365546808948 32.21668750197405 20.181631086431203 24-25 20.981990920503478 26.459740203264452 32.48349520562759 20.074773670604472 26-27 20.718603304517792 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21.407611263162863 52-53 21.374343750858966 27.851099016886106 29.463731068414834 21.310826163840098 54-55 21.038178652586584 27.898234233309864 29.539386321755313 21.52420079234824 56-57 21.12819207139277 28.191109744451115 29.175082043484363 21.505616140671748 58-59 21.144237436387314 27.67141230863544 29.48890779728822 21.695442457689023 60-61 21.407077895858684 27.930559637571882 29.500365779182907 21.161996687386527 62-63 21.289450031276445 27.85688504024739 29.60960178782693 21.244063140649235 64-65 21.152844333032423 27.621145313950574 29.72505177424051 21.5009585787765 66-67 20.74917806912608 28.04615453564497 29.75623041414284 21.448436981086108 68-69 20.829690867406395 27.81835728997305 29.971582421015302 21.38036942160525 70-71 20.466983484814897 27.78423575703835 30.305226113943696 21.443554644203058 72-73 21.044322100183976 28.14525427898898 29.110390754960175 21.70003286586687 74-75 20.621590851005646 28.392863241623495 29.15658596527983 21.828959942091025 76-77 20.84255460196625 28.468700183402905 29.09836365783958 21.590381556791264 78-79 20.768012862169105 28.344748798349013 28.959153594561045 21.928084744920838 80-81 20.497761449042038 28.908629447433476 28.685083338273305 21.90852576525118 82-83 20.835734619675154 28.621647144342216 28.497161713875307 22.045456522107322 84-85 20.558062760431806 28.382792584247955 28.549345347165357 22.509799308154882 86-87 20.625390663175846 28.40624530088417 28.63896668774617 22.32939734819381 88-89 20.713534995839005 28.273613070557463 28.543436843979652 22.469415089623883 90-91 20.55796875550752 28.530294852903143 28.714271785061474 22.197464606527863 92-93 20.65416293305593 28.39617815588812 28.489469787221385 22.46018912383457 94-95 20.145679338008865 28.184536336684197 28.826204389189314 22.843579936117624 96-97 20.195305689878236 28.197035317434832 28.776345852105372 22.831313140581557 98-99 20.41344781267483 28.146114072187178 28.3502329844442 23.09020513069379 100-101 20.18906642159752 28.095361806980577 28.68496979171793 23.03060197970397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5993.0 1 4016.5 2 2203.5 3 2407.5 4 2328.5 5 2432.5 6 3885.0 7 7475.5 8 10831.0 9 12181.5 10 11082.5 11 8891.5 12 7835.0 13 7418.5 14 7224.0 15 7049.0 16 6913.5 17 6849.5 18 7043.5 19 7280.0 20 7544.5 21 8044.0 22 8788.0 23 9893.0 24 10978.0 25 12255.0 26 14106.0 27 16089.5 28 17992.0 29 20948.0 30 24999.0 31 29246.5 32 34748.5 33 41284.0 34 47097.0 35 51975.0 36 59007.0 37 65064.0 38 66725.0 39 67032.5 40 66991.5 41 68080.0 42 68984.5 43 69939.5 44 71180.5 45 71583.5 46 70880.5 47 70496.0 48 73837.0 49 74798.0 50 71329.0 51 67805.5 52 64752.0 53 63267.5 54 61534.5 55 57745.0 56 52887.5 57 47915.5 58 43962.5 59 40299.5 60 35893.0 61 30540.0 62 24601.0 63 18766.0 64 13858.5 65 10273.0 66 7618.5 67 5912.5 68 4342.5 69 2918.0 70 2140.5 71 1727.0 72 1466.0 73 1258.0 74 980.0 75 728.0 76 478.0 77 263.0 78 157.0 79 95.5 80 60.5 81 34.0 82 23.0 83 11.5 84 9.0 85 5.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021285429368307966 2 0.019957950977596284 3 0.0 4 0.0 5 1.373253507632772E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.348636107503778E-4 18-19 0.0012588157153300408 20-21 2.28875584605462E-5 22-23 0.0 24-25 2.28875584605462E-5 26-27 3.662009353687392E-4 28-29 0.017692082690002213 30-31 0.0044630738998065085 32-33 0.00823952104579663 34-35 0.009132135825757932 36-37 0.10253626190324698 38-39 0.10450459193085393 40-41 0.11922129202098515 42-43 0.10409261587856411 44-45 0.09967531709567869 46-47 0.10079680746024546 48-49 0.10331443889090552 50-51 0.1017123097986673 52-53 0.07932827762425312 54-55 0.06692322093863709 56-57 0.03856553600602034 58-59 0.01732588175463347 60-61 0.009841650138034865 62-63 0.0018996673522253347 64-65 0.004989487744399071 66-67 0.41543207361737405 68-69 0.7987071275976807 70-71 0.8840548330970575 72-73 0.2765274813203192 74-75 0.14824271614895773 76-77 0.015151563700881584 78-79 0.00926946117652121 80-81 0.0014876912999355028 82-83 0.0 84-85 0.0 86-87 2.9753825998710056E-4 88-89 8.697272215007556E-4 90-91 0.003662009353687392 92-93 0.002082767819909704 94-95 0.2199723243643095 96-97 0.22580865177174878 98-99 0.014968463233197213 100-101 0.008033533019651715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2184593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.048686709700526 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2331760269647 27.03464600594494 2 14.12672838846222 11.032603821481878 3 5.885273244434399 6.8943657336870485 4 3.113811088459643 4.863609346658087 5 1.8793495721984497 3.6693066331393487 6 1.1985411219112423 2.8080874066923047 7 0.819903238618154 2.2411301287948393 8 0.5984754917992509 1.8695745586162316 9 0.4367616510675266 1.5349471991412647 >10 2.355172649492291 16.84257526142385 >50 0.2069283612190372 5.639916308726969 >100 0.13850909564884747 9.877788475274398 >500 0.00534924415470151 1.419508580260885 >1k 0.0014264651079204027 0.9979868047352612 >5k 2.3774418465340042E-4 0.5779968229099289 >10k+ 3.5661627698010067E-4 2.695956912512743 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23191 1.061570736517054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20533 0.93990047574079 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14356 0.6571475785192025 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7194 0.32930619113033865 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 0.24068556477110384 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4348 0.19903020837290972 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3368 0.15417059379023917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.57751169210924E-5 0.0 6 0.0 0.0 0.0 4.57751169210924E-5 0.0 7 0.0 0.0 0.0 4.57751169210924E-5 0.0 8 0.0 0.0 0.0 4.57751169210924E-5 0.0 9 0.0 0.0 0.0 4.57751169210924E-5 0.0 10-11 0.0 0.0 0.0 4.57751169210924E-5 0.0 12-13 0.0 0.0 0.0 4.57751169210924E-5 0.0 14-15 0.0 0.0 0.0 6.86626753816386E-5 0.0 16-17 0.0 0.0 0.0 9.15502338421848E-5 0.0 18-19 0.0 0.0 0.0 1.602129092238234E-4 0.0 20-21 0.0 0.0 0.0 4.3486361075037775E-4 0.0 22-23 0.0 0.0 0.0 0.0010070525722640326 0.0 24-25 0.0 0.0 0.0 0.00162501665069878 0.0 26-27 0.0 0.0 0.0 0.002380306079896805 0.0 28-29 0.0 0.0 0.0 0.004485961458267055 0.0 30-31 0.0 0.0 0.0 0.010116300839561419 0.0 32-33 0.0 0.0 0.0 0.020072388769899016 0.0 34-35 0.0 0.0 0.0 0.037054957147624294 0.0 36-37 0.0 0.0 0.0 0.0632154364680286 0.0 38-39 0.0 0.0 0.0 0.10505389333390705 0.0 40-41 0.0 0.0 0.0 0.15394171820563374 0.0 42-43 0.0 0.0 0.0 0.20356194494809787 0.0 44-45 0.0 0.0 0.0 0.2610326042425294 0.0 46-47 0.0 0.0 0.0 0.32873400216882503 0.0 48-49 0.0 0.0 0.0 0.40909221992380274 0.0 50-51 0.0 0.0 0.0 0.49512655217699586 0.0 52-53 0.0 0.0 0.0 0.5821908245609136 0.0 54-55 0.0 0.0 0.0 0.6770368668214171 0.0 56-57 0.0 0.0 0.0 0.7800995425692566 0.0 58-59 0.0 0.0 0.0 0.8854280866046902 0.0 60-61 0.0 0.0 0.0 0.998767276101315 0.0 62-63 0.0 0.0 0.0 1.1108476498826096 0.0 64-65 0.0 4.57751169210924E-5 0.0 1.2257660809130122 0.0 66-67 0.0 4.57751169210924E-5 0.0 1.3475050043646575 0.0 68-69 0.0 4.57751169210924E-5 0.0 1.4899800557815575 0.0 70-71 0.0 4.57751169210924E-5 0.0 1.686652845633031 0.0 72-73 0.0 4.57751169210924E-5 0.0 1.9020705458636917 0.0 74-75 0.0 4.57751169210924E-5 0.0 2.1256133293478463 0.0 76-77 0.0 4.57751169210924E-5 0.0 2.3695031523034267 0.0 78-79 0.0 4.57751169210924E-5 0.0 2.6440394160376783 0.0 80-81 0.0 4.57751169210924E-5 0.0 2.9616271772362177 0.0 82-83 0.0 4.57751169210924E-5 0.0 3.2932907868879924 0.0 84-85 0.0 4.57751169210924E-5 0.0 3.614563444998679 0.0 86-87 0.0 4.57751169210924E-5 0.0 3.939154799086146 0.0 88-89 0.0 4.57751169210924E-5 0.0 4.304394456999542 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9925 0.0 54.059914 1 GTATCAA 17660 0.0 41.439644 1 ATCAACG 23960 0.0 30.432825 3 TATCAAC 24000 0.0 30.429245 2 TCAACGC 24445 0.0 29.809639 4 CAACGCA 24900 0.0 29.245895 5 AACGCAG 25465 0.0 28.615614 6 GTGGTAT 4640 0.0 28.295752 1 TGGTATC 4675 0.0 27.573193 2 ACGCAGA 29095 0.0 24.996572 7 CGCAGAG 29255 0.0 24.811275 8 GTACATG 18240 0.0 24.504524 1 TACATGG 18630 0.0 23.784746 2 TACACCG 160 1.9024046E-6 23.689747 5 GCAGAGT 29890 0.0 23.475754 9 GAGTACT 17085 0.0 22.782057 12-13 ACATGGG 18725 0.0 22.443584 3 AGTACTT 17565 0.0 21.700926 12-13 GTACTTT 18210 0.0 21.270523 14-15 CATGGGA 13100 0.0 20.54317 4 >>END_MODULE