FastQCFastQC Report
Fri 27 May 2016
SRR939482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181199
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161940.7424356970638626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115950.5315883603467634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83220.3815332759642747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45530.20873840488648673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.13785995684025162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.11929218746203349No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.10145795959011536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79250.058.0691071
GTATCAA152150.047.075591
TATCAAC202750.034.7861442
ATCAACG210200.034.0204053
TCAACGC215100.033.5130124
CAACGCA214500.032.9650735
AACGCAG219000.032.1251036
ACGCAGA243250.028.3054897
CGCAGAG245600.027.7593658
TGGTATC37250.027.0672512
GTACATG161800.026.8224411
GTGGTAT38600.026.3777481
GCAGAGT239350.026.2953139
TACATGG166300.025.5464572
GAGTACT132650.024.7474612-13
CGCATAG3750.024.5857758
ACATGGG173300.023.7421993
AGTACTT139350.023.52307512-13
CAACGCG4800.023.2705445
GTACTTT147800.022.59896714-15
Produced by FastQC (version 0.11.5)