FastQCFastQC Report
Fri 27 May 2016
SRR939482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181199
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229351.051485902936871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197950.9075283823255007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141320.6479005354394533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71740.3289016728872515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.23115726717277976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44170.20250330208293693No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34210.1568403433157635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96050.055.734681
GTATCAA170600.040.809841
TATCAAC229050.030.2258822
ATCAACG230100.030.0191963
TCAACGC233300.029.614244
CAACGCA238400.028.9528065
AACGCAG245450.028.198466
TGGTATC44200.027.0540472
GTGGTAT46700.026.2190281
ACGCAGA281850.024.4221637
CGCAGAG283700.024.2294868
GTACATG176500.023.8771251
GAGTACT162600.023.1147212-13
TACATGG181350.022.8690382
GCAGAGT289300.022.7445099
ACATGGG183600.021.6533783
GTACTTT175550.021.5310814-15
AGTACTT169300.021.27612712-13
TATAACG1404.8869016E-420.3365462
CAGAGTA286400.020.19168510-11
Produced by FastQC (version 0.11.5)