##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939482_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2181199 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.896631623249416 31.0 31.0 31.0 28.0 34.0 2 31.136212697695168 31.0 31.0 34.0 28.0 34.0 3 31.12034252720637 31.0 31.0 34.0 28.0 34.0 4 35.054386601130844 35.0 35.0 37.0 32.0 37.0 5 34.64008235837262 35.0 35.0 37.0 32.0 37.0 6 34.720549110833076 35.0 35.0 37.0 32.0 37.0 7 34.54721921291913 35.0 35.0 37.0 30.0 37.0 8 34.45952249198721 35.0 35.0 37.0 30.0 37.0 9 35.91940533623938 37.0 35.0 39.0 30.0 39.0 10-11 35.73233460129039 37.0 35.0 39.0 30.0 39.0 12-13 35.70358252502408 37.0 35.0 39.0 30.0 39.0 14-15 36.450033674139775 38.0 35.0 40.0 30.0 41.0 16-17 36.38795703647398 38.0 35.0 40.0 30.0 41.0 18-19 36.48150145860144 38.0 35.0 40.0 30.0 41.0 20-21 36.41216161386467 38.0 34.5 40.0 30.0 41.0 22-23 36.24916250190836 38.0 34.0 40.0 30.0 41.0 24-25 36.07951085618507 38.0 34.0 40.0 29.0 41.0 26-27 35.605447279225785 38.0 34.0 40.0 27.0 41.0 28-29 35.388364381241686 38.0 34.0 40.0 27.0 41.0 30-31 35.02941432670747 37.0 33.0 40.0 27.0 41.0 32-33 34.669774513925596 37.0 33.0 40.0 25.5 41.0 34-35 34.81437984337972 37.0 33.0 40.0 26.0 41.0 36-37 34.58638620318458 37.0 33.0 40.0 25.0 41.0 38-39 34.3671006175961 37.0 33.0 40.0 25.0 41.0 40-41 34.166207439119496 37.0 33.0 40.0 24.0 41.0 42-43 33.9302106318589 37.0 33.0 40.0 24.0 41.0 44-45 33.43662545233149 37.0 32.0 40.0 22.5 41.0 46-47 32.88738258178186 37.0 31.0 40.0 19.0 41.0 48-49 32.39470034600236 36.0 31.0 39.0 16.0 41.0 50-51 32.11651068976283 36.0 30.0 39.0 14.0 40.0 52-53 31.99508160420026 36.0 30.5 39.0 13.5 40.0 54-55 31.652246539632557 35.5 30.0 39.0 10.5 40.0 56-57 31.162858822143235 35.0 29.5 39.0 8.0 40.0 58-59 30.722244508639513 35.0 28.0 38.0 7.5 40.0 60-61 30.35840287841687 34.5 28.0 38.0 6.5 40.0 62-63 30.194969372349796 34.0 28.0 38.0 3.5 40.0 64-65 29.75811331290726 34.0 28.0 38.0 2.0 40.0 66-67 28.92500798872547 33.5 27.0 37.0 2.0 39.5 68-69 25.622845508364897 31.0 15.5 35.5 2.0 39.0 70-71 24.376069996364386 29.5 11.5 35.0 2.0 38.0 72-73 25.335618620767754 30.0 16.5 35.0 2.0 37.5 74-75 26.045877061194325 31.0 21.0 35.0 2.0 37.0 76-77 25.68069809311301 30.5 21.0 34.0 2.0 36.0 78-79 26.744299580185025 32.0 24.5 35.0 2.0 36.0 80-81 26.756583649634905 32.0 25.0 35.0 2.0 36.0 82-83 26.441998414633417 32.0 24.0 35.0 2.0 36.0 84-85 26.090604525309246 32.0 23.5 34.5 2.0 35.5 86-87 25.65406136716549 31.5 22.0 34.0 2.0 35.0 88-89 25.186900186548776 31.0 20.0 34.0 2.0 35.0 90-91 24.6053833235757 31.0 18.5 34.0 2.0 35.0 92-93 24.059759563432774 31.0 14.0 34.0 2.0 35.0 94-95 23.24621435274819 30.0 4.5 34.0 2.0 35.0 96-97 22.601799973317426 30.0 2.0 34.0 2.0 35.0 98-99 21.78835860460233 29.5 2.0 34.0 2.0 35.0 100-101 20.439160984394363 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23298562927353927 1101 2 0.16218351531748354 1101 3 0.3262315432193148 1101 4 0.33932769603790547 1101 5 0.3854537481858955 1101 6 0.447618029840541 1101 7 0.657576834044221 1101 8 0.6228118280608399 1101 9 0.592115223101203 1101 10-11 0.6890576812465454 1101 12-13 0.6954923788658078 1101 14-15 0.8187639154507877 1101 16-17 0.7248884484015576 1101 18-19 0.6068208536153037 1101 20-21 0.7619581919352072 1101 22-23 0.6852241929388114 1101 24-25 0.7536899051092547 1101 26-27 0.6950478147510069 1101 28-29 0.7073207105106718 1101 30-31 0.4194419507455507 1101 32-33 0.9291634635460966 1101 34-35 0.9518260823583731 1101 36-37 1.1056018556946015 1101 38-39 1.18083859166817 1101 40-41 1.1950419657548323 1101 42-43 0.8274190435926698 1101 44-45 0.8756279929841284 1101 46-47 0.7374160328976984 1101 48-49 0.6937037754561217 1101 50-51 0.4578077517777004 1101 52-53 0.34054576185640784 1101 54-55 0.36644455130157283 1101 56-57 0.4733067481612956 1101 58-59 0.4492610309918703 1101 60-61 0.31764408843615755 1101 62-63 0.14409286122979736 1101 64-65 -0.005278830829936965 1101 66-67 -0.20818498247417594 1101 68-69 -6.850444816526405 1101 70-71 -7.735496245029882 1101 72-73 -4.699809326973767 1101 74-75 -2.798206232054067 1101 76-77 -0.35821254159957405 1101 78-79 -0.24141812571504673 1101 80-81 -0.31094570213817363 1101 82-83 -0.3452583869142316 1101 84-85 -0.3940481531361577 1101 86-87 -0.5244385681544905 1101 88-89 -0.5951268963524967 1101 90-91 -0.7595293988240144 1101 92-93 -0.6342344871281327 1101 94-95 -0.41402137350025114 1101 96-97 -0.5169505309387361 1101 98-99 -0.6144999275927816 1101 100-101 -0.5411640422160282 1102 1 0.2702925652499921 1102 2 0.373796240092485 1102 3 0.32703958858150983 1102 4 0.30702908249366345 1102 5 0.5546602233517319 1102 6 0.4295744022787602 1102 7 0.38980985054570993 1102 8 0.6505723007945647 1102 9 0.5038051816421998 1102 10-11 0.6899203757062509 1102 12-13 0.7106824716278979 1102 14-15 0.8136458416801275 1102 16-17 0.8021148734787289 1102 18-19 0.6439399163261825 1102 20-21 0.6381399220259425 1102 22-23 0.6411325216606514 1102 24-25 0.6405105366208161 1102 26-27 0.7185485579968613 1102 28-29 0.6255772611067627 1102 30-31 0.6415429520656488 1102 32-33 0.912504732931346 1102 34-35 0.7018711701295359 1102 36-37 0.8685954263143785 1102 38-39 0.8387047900141269 1102 40-41 0.8950623753348594 1102 42-43 0.6893399874650541 1102 44-45 0.9868245870856143 1102 46-47 0.9048895969632014 1102 48-49 0.8483076130009479 1102 50-51 0.6455249547516999 1102 52-53 0.40108436451070517 1102 54-55 0.2333042147755151 1102 56-57 0.511532296496565 1102 58-59 0.5443790086961258 1102 60-61 0.5056839295314433 1102 62-63 0.4894120799307764 1102 64-65 0.2267607840561503 1102 66-67 -0.036949240405594 1102 68-69 -6.821466928656928 1102 70-71 -7.337107496182455 1102 72-73 -1.4028523844811112 1102 74-75 0.2137394443866647 1102 76-77 0.6488427203291103 1102 78-79 0.30765467938959645 1102 80-81 0.14401184104141151 1102 82-83 -0.009112422528044561 1102 84-85 -0.28423156203266586 1102 86-87 -0.34094738079726383 1102 88-89 -0.30046086577836917 1102 90-91 -0.3352220240234658 1102 92-93 -0.14270718170202912 1102 94-95 -0.20125249858106642 1102 96-97 -0.4760851502447849 1102 98-99 -0.4502102398865446 1102 100-101 -0.6082542831922666 1103 1 0.40385896349351214 1103 2 0.2957247968768897 1103 3 0.30505211305306545 1103 4 0.3003153868656909 1103 5 0.33093661116255646 1103 6 0.43552150754371155 1103 7 0.4134713804510355 1103 8 0.500436993925085 1103 9 0.5095248965600732 1103 10-11 0.5582142612130241 1103 12-13 0.48566678938431807 1103 14-15 0.62706673107526 1103 16-17 0.5960809543894925 1103 18-19 0.6454652318980152 1103 20-21 0.5189305762651983 1103 22-23 0.46222648071535843 1103 24-25 0.48930216054619535 1103 26-27 0.6438583445732817 1103 28-29 0.5195190649082164 1103 30-31 0.5574051154130188 1103 32-33 0.6469923329914096 1103 34-35 0.5551016249154728 1103 36-37 0.845717527939513 1103 38-39 0.9056614594813723 1103 40-41 0.6934140662294084 1103 42-43 0.5374359821964418 1103 44-45 0.8468579633044371 1103 46-47 0.7576567067759612 1103 48-49 0.7160227979395657 1103 50-51 0.4645702159176395 1103 52-53 0.35862118348229544 1103 54-55 0.38496335157658734 1103 56-57 0.3572475090506124 1103 58-59 0.49899103899130637 1103 60-61 0.3759374619032805 1103 62-63 0.06627552285133831 1103 64-65 0.11570017500308083 1103 66-67 0.0755214160149329 1103 68-69 -6.938204343842163 1103 70-71 -7.982258480052074 1103 72-73 -1.4017090532317518 1103 74-75 0.27481708586811493 1103 76-77 0.5476739409363702 1103 78-79 0.19470984156121318 1103 80-81 -0.03712877760365174 1103 82-83 -0.1571045622677154 1103 84-85 -0.44030514771626983 1103 86-87 -0.3854864381917409 1103 88-89 -0.16567099910322014 1103 90-91 -0.4605223199804769 1103 92-93 -0.23023428808208735 1103 94-95 -0.14369643619885863 1103 96-97 -0.14595089555816187 1103 98-99 0.03356840391268179 1103 100-101 0.1414378834112746 1104 1 0.26414642242875175 1104 2 0.23809159304338934 1104 3 0.20591653177919866 1104 4 0.16305349522303203 1104 5 0.4019538790143997 1104 6 0.33371263163987663 1104 7 0.40398593577371855 1104 8 0.5507518790937951 1104 9 0.5428332060207026 1104 10-11 0.500512159811322 1104 12-13 0.4564883674473279 1104 14-15 0.6913949849590892 1104 16-17 0.5307497623132491 1104 18-19 0.5623775240002544 1104 20-21 0.5030495574503888 1104 22-23 0.5712548559934305 1104 24-25 0.4708042413562268 1104 26-27 0.6600880764867227 1104 28-29 0.5491136316063248 1104 30-31 0.6499332560599385 1104 32-33 0.8113863284346792 1104 34-35 0.5539982290672967 1104 36-37 0.9645529848528369 1104 38-39 0.9920363315810974 1104 40-41 1.026503369377508 1104 42-43 1.0296159788610808 1104 44-45 1.0913937135541119 1104 46-47 1.293273903953903 1104 48-49 1.379757653193444 1104 50-51 1.2206756700698875 1104 52-53 1.0476502416999658 1104 54-55 1.0213268154807267 1104 56-57 1.2362779521180265 1104 58-59 1.2149343846675649 1104 60-61 1.3631684746733903 1104 62-63 1.1022297058822552 1104 64-65 1.1303814793418319 1104 66-67 1.0763611212954558 1104 68-69 -5.860426146412948 1104 70-71 -3.5491610368158213 1104 72-73 0.9670668620409657 1104 74-75 1.653513129974975 1104 76-77 1.6223943452917986 1104 78-79 1.1428911095972403 1104 80-81 0.9019034279997982 1104 82-83 0.8783796686396848 1104 84-85 0.8765071696167901 1104 86-87 0.8438377810022075 1104 88-89 0.9225231482078868 1104 90-91 0.9304050688810186 1104 92-93 0.8500188755579998 1104 94-95 1.0753795499719594 1104 96-97 0.880636322468412 1104 98-99 0.7499774764548377 1104 100-101 0.6633838715119147 1105 1 0.08712227847246368 1105 2 0.16870836927979127 1105 3 0.10892945811165333 1105 4 0.25208783350871755 1105 5 0.4333921869101829 1105 6 0.31434147502756105 1105 7 0.2645746196819303 1105 8 0.4326231563776233 1105 9 0.48921450869382 1105 10-11 0.4958851313231989 1105 12-13 0.3392004838325917 1105 14-15 0.4136197815069238 1105 16-17 0.510896885021694 1105 18-19 0.6219236060375763 1105 20-21 0.5954231178392391 1105 22-23 0.46555143761081297 1105 24-25 0.4890024708637881 1105 26-27 0.535593690125495 1105 28-29 0.5769568937917384 1105 30-31 0.45617631744795517 1105 32-33 0.693069358158489 1105 34-35 0.616970416697022 1105 36-37 0.7530042383885132 1105 38-39 0.5474718592052241 1105 40-41 0.7151245284032512 1105 42-43 0.5958318167557977 1105 44-45 0.7695560312194587 1105 46-47 0.6943000944990487 1105 48-49 0.7880386150902083 1105 50-51 0.49115845265843916 1105 52-53 0.3904220180811713 1105 54-55 0.5541002329945073 1105 56-57 0.6820800315295337 1105 58-59 0.8571906523459418 1105 60-61 0.7981236331392196 1105 62-63 0.3276344381363039 1105 64-65 0.5684644434203356 1105 66-67 0.5002656653019422 1105 68-69 -2.4019342507117507 1105 70-71 -2.516705226723822 1105 72-73 0.9693248673891297 1105 74-75 1.215259295034297 1105 76-77 1.1508322766924586 1105 78-79 0.7718590302822683 1105 80-81 0.5807569520906561 1105 82-83 0.4090215445507539 1105 84-85 0.4693276972891809 1105 86-87 0.6615398092489926 1105 88-89 0.7835240460039437 1105 90-91 0.7736740118951033 1105 92-93 0.6131368343752293 1105 94-95 0.5734980397438427 1105 96-97 0.6443151624772909 1105 98-99 0.6403681625447888 1105 100-101 0.7341543349870605 1106 1 0.08724731164543797 1106 2 0.10778604480267617 1106 3 0.062209085363186034 1106 4 0.16718745929975398 1106 5 0.1976889821781569 1106 6 0.18457378381318534 1106 7 0.27342597091293896 1106 8 0.37977936413216895 1106 9 0.2776565989207498 1106 10-11 0.34583935707041746 1106 12-13 0.17787340453271128 1106 14-15 0.03546959564920371 1106 16-17 0.12011923924317358 1106 18-19 0.23481306566985438 1106 20-21 0.17541611811886781 1106 22-23 0.295118487985647 1106 24-25 0.22709261586309282 1106 26-27 0.3897060703688311 1106 28-29 0.4416590672672456 1106 30-31 0.3674915524372935 1106 32-33 0.3206803161940073 1106 34-35 0.2254294808005639 1106 36-37 0.18374147102323946 1106 38-39 0.4465116685563473 1106 40-41 0.38831711333051544 1106 42-43 0.3615022848821354 1106 44-45 0.39396020896604256 1106 46-47 0.6141309434956668 1106 48-49 0.7863829942078837 1106 50-51 0.5952325392661777 1106 52-53 0.34761801559475103 1106 54-55 0.24764526185722247 1106 56-57 0.5641171104553884 1106 58-59 0.6783616895335101 1106 60-61 0.7673856880647598 1106 62-63 0.36045208384358673 1106 64-65 0.4905696672065609 1106 66-67 0.6429700152808344 1106 68-69 -2.7337252707651487 1106 70-71 -2.861249096090873 1106 72-73 0.9379963097767963 1106 74-75 1.4580101317057093 1106 76-77 1.303455746950771 1106 78-79 0.9603867023320127 1106 80-81 0.9667163679613218 1106 82-83 0.727290992819615 1106 84-85 0.5440822172740063 1106 86-87 0.5558388665131204 1106 88-89 0.5349039527099606 1106 90-91 0.6050727448210402 1106 92-93 0.5968748631613536 1106 94-95 0.9178276428614787 1106 96-97 0.9609142957943568 1106 98-99 0.7642187746195717 1106 100-101 0.902044409098604 1107 1 -0.00743674036490205 1107 2 0.028714091144166787 1107 3 0.08517659146674106 1107 4 0.007709748066481836 1107 5 0.22121412175953026 1107 6 0.23496221504925785 1107 7 0.03991777158828569 1107 8 0.08331540142332017 1107 9 0.19639617971586887 1107 10-11 0.11039974629930782 1107 12-13 0.11608981877097335 1107 14-15 -0.09858734998353214 1107 16-17 -0.03816307481556436 1107 18-19 0.03605734227790691 1107 20-21 0.07798660448380446 1107 22-23 0.2116775251992138 1107 24-25 0.09474526666178917 1107 26-27 0.4324104688986594 1107 28-29 0.3447306406714503 1107 30-31 0.43340439406975406 1107 32-33 0.31790398622359106 1107 34-35 0.12640719695092173 1107 36-37 0.2184339403488451 1107 38-39 0.4365208871738062 1107 40-41 0.2509712774976691 1107 42-43 0.08214166211007523 1107 44-45 0.461613151147354 1107 46-47 0.6330627093362295 1107 48-49 0.4261172569060676 1107 50-51 0.3050251076254469 1107 52-53 0.10875648024877549 1107 54-55 -0.08303512383137246 1107 56-57 0.37412435495562946 1107 58-59 0.4327336124875778 1107 60-61 0.38555399503343324 1107 62-63 -0.029850921294578114 1107 64-65 -0.14943658295051065 1107 66-67 0.1474738799248705 1107 68-69 -2.841698009007768 1107 70-71 -2.7365134802846747 1107 72-73 0.7647779960881174 1107 74-75 0.9900776989631872 1107 76-77 0.529269988348883 1107 78-79 0.3132019683042415 1107 80-81 0.29013304036458365 1107 82-83 0.24858887654411177 1107 84-85 0.1163567608868803 1107 86-87 0.10509891857590858 1107 88-89 0.3597911967439913 1107 90-91 0.3403978058019561 1107 92-93 0.36531091432959784 1107 94-95 0.6261088460239996 1107 96-97 0.4460142637058482 1107 98-99 0.6023170332078038 1107 100-101 0.8354324313063302 1108 1 -0.08999861658383779 1108 2 -0.013112684069671587 1108 3 0.049045826916774615 1108 4 0.0018983124442470967 1108 5 -0.043025721394649 1108 6 -0.3545598412105946 1108 7 0.02984919191350599 1108 8 0.14546320849591154 1108 9 0.12556360793035282 1108 10-11 0.25552378500575657 1108 12-13 0.15350035066533252 1108 14-15 0.2331140689543787 1108 16-17 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1.3076577994884993 2310 100-101 1.5177877982591461 2311 1 -0.26822566147852456 2311 2 -0.22585648847672601 2311 3 -0.1859836480283974 2311 4 0.007624197705872859 2311 5 0.24391478380005793 2311 6 0.3067304813700531 2311 7 -3.2533912957178295 2311 8 -0.8971405842922948 2311 9 -3.197406809042178 2311 10-11 -1.3528528187780253 2311 12-13 -0.8332172347759794 2311 14-15 -1.8561297151872438 2311 16-17 0.14696779086004597 2311 18-19 0.3734828727532147 2311 20-21 -0.813072236045052 2311 22-23 0.06356232808361284 2311 24-25 0.35802799225498916 2311 26-27 0.7773184449216259 2311 28-29 0.6865990559599666 2311 30-31 0.4619271824610536 2311 32-33 1.0562441945490448 2311 34-35 0.49980137883832754 2311 36-37 0.7992057513903461 2311 38-39 0.7488368134170074 2311 40-41 0.7063762598314014 2311 42-43 0.8569687551982668 2311 44-45 0.8067473883787315 2311 46-47 0.8970322460684059 2311 48-49 0.9851864552212035 2311 50-51 0.8605658696199896 2311 52-53 1.1207796315983707 2311 54-55 1.1306442182794711 2311 56-57 1.038142974200337 2311 58-59 1.025695203127956 2311 60-61 1.3700955265225012 2311 62-63 1.3704364636969508 2311 64-65 1.503840534850955 2311 66-67 1.5540551797169577 2311 68-69 3.8915230538121115 2311 70-71 5.195680863529152 2311 72-73 4.18954402273258 2311 74-75 3.0401969075420787 2311 76-77 2.7885866634444376 2311 78-79 2.229710320932277 2311 80-81 1.9442716250680405 2311 82-83 1.6809990457985045 2311 84-85 1.5321469950723916 2311 86-87 1.7700183395398739 2311 88-89 2.0391131241271054 2311 90-91 1.8741170331881634 2311 92-93 1.9980965418690175 2311 94-95 2.2168832832092775 2311 96-97 2.2205282837585756 2311 98-99 2.5762803658186613 2311 100-101 2.6960702058042934 2312 1 -0.23321927737472947 2312 2 -0.14724015520214806 2312 3 -0.10321528280320535 2312 4 0.07695685465379398 2312 5 -1.123631900406771 2312 6 -0.24594374877581515 2312 7 0.11007930659477694 2312 8 0.197909048353047 2312 9 -2.453004256547075 2312 10-11 -0.1791100429897554 2312 12-13 -1.3520709048116757 2312 14-15 -4.200734610096731 2312 16-17 -1.1432869570932382 2312 18-19 -0.0895406273406465 2312 20-21 0.10080527227721348 2312 22-23 0.16913032702890973 2312 24-25 0.24029809644986955 2312 26-27 0.3369518497626487 2312 28-29 0.17670949880611175 2312 30-31 0.007010276121796721 2312 32-33 0.4146922053024227 2312 34-35 0.26009698218987154 2312 36-37 0.492209604780939 2312 38-39 0.45135319641737937 2312 40-41 0.1263840841202324 2312 42-43 -0.33529666302143823 2312 44-45 0.18852138107320116 2312 46-47 0.4057344029067025 2312 48-49 0.4239860430846747 2312 50-51 0.4751942803865532 2312 52-53 0.41527253600017744 2312 54-55 0.4628215344337576 2312 56-57 0.4361530827229352 2312 58-59 0.4543176267372431 2312 60-61 0.5379667897439013 2312 62-63 0.4649312310246678 2312 64-65 0.7690773342295998 2312 66-67 1.0947800298588994 2312 68-69 3.632182271641174 2312 70-71 4.481694015786083 2312 72-73 2.6542239505656973 2312 74-75 2.1181460267363796 2312 76-77 1.9453485829459787 2312 78-79 1.3870968938221218 2312 80-81 1.1180596842861554 2312 82-83 1.118497594865822 2312 84-85 1.1797150885970034 2312 86-87 1.0678424420753174 2312 88-89 1.0513485349094296 2312 90-91 1.190285797079504 2312 92-93 1.4800821916074192 2312 94-95 1.6555331204898636 2312 96-97 1.6600928176616776 2312 98-99 1.9873292360973949 2312 100-101 1.8452346835330147 2313 1 -0.25262873449639045 2313 2 -0.19293526515490456 2313 3 -0.1675191394046145 2313 4 0.0010263122963820592 2313 5 0.1503959253324112 2313 6 0.030421512992653277 2313 7 -2.9549815700865665 2313 8 -3.3854341573287208 2313 9 -0.843140982271386 2313 10-11 -0.12527843752744872 2313 12-13 -0.06306021494877001 2313 14-15 -0.5118946488467486 2313 16-17 -0.3427478848587384 2313 18-19 0.022256787028283043 2313 20-21 -2.446848343564916 2313 22-23 -0.7519757456836658 2313 24-25 -0.17441195477711346 2313 26-27 -1.5138836869948378 2313 28-29 -1.6956708630343584 2313 30-31 -1.1751866448817694 2313 32-33 -1.0212333126597954 2313 34-35 -1.39680026052622 2313 36-37 -2.134652173098779 2313 38-39 -1.6689466205030712 2313 40-41 -0.9719562690829946 2313 42-43 -0.46651697770988676 2313 44-45 -0.19601541253926058 2313 46-47 -0.4067481104335684 2313 48-49 -2.2072569570001868 2313 50-51 -0.6450572250415583 2313 52-53 -0.48698239240219365 2313 54-55 -0.44987371751277294 2313 56-57 -0.5360426031868322 2313 58-59 -0.22578719659812663 2313 60-61 -0.014286920368657974 2313 62-63 0.15224917021604512 2313 64-65 -0.0788439692900802 2313 66-67 0.5081491220985619 2313 68-69 1.301881219626921 2313 70-71 3.784052540832022 2313 72-73 3.0987564759628405 2313 74-75 1.7309466397461968 2313 76-77 1.7590023349004937 2313 78-79 1.0825723335197175 2313 80-81 0.826151985282717 2313 82-83 0.7394317626856655 2313 84-85 0.610234760616585 2313 86-87 0.880895871225043 2313 88-89 0.4849933303333671 2313 90-91 0.9327720997211735 2313 92-93 0.9531046341860616 2313 94-95 1.1820069780911133 2313 96-97 1.362282714594361 2313 98-99 1.5098566749245457 2313 100-101 1.500989674530274 2314 1 -0.2724499405010441 2314 2 -0.14527773121051624 2314 3 -0.1425853185515642 2314 4 -0.028973522255753892 2314 5 -0.2984451123566103 2314 6 -2.571461007991651 2314 7 -0.7457892865750964 2314 8 -0.053262543770820514 2314 9 -0.08285040066698457 2314 10-11 -0.3022115579845206 2314 12-13 -2.366125412438379 2314 14-15 -0.7640195509567604 2314 16-17 -1.8144888376178585 2314 18-19 -0.47570020755605924 2314 20-21 -0.7775757351138495 2314 22-23 -0.37346783727560506 2314 24-25 -0.14410107823431417 2314 26-27 -1.853440203986331 2314 28-29 -0.8090913046698702 2314 30-31 -0.15814096472558958 2314 32-33 -0.003738027924008236 2314 34-35 -0.13351314229716849 2314 36-37 -0.5313363220900982 2314 38-39 -0.23718807512072004 2314 40-41 -0.425786249339545 2314 42-43 -0.8078218650398981 2314 44-45 -3.6970649035113254 2314 46-47 -4.208184611542066 2314 48-49 -4.018269580672467 2314 50-51 -4.208271959828629 2314 52-53 -1.3181122358885098 2314 54-55 -1.7038082056722175 2314 56-57 -1.4665412630591028 2314 58-59 -1.9698554769329952 2314 60-61 -1.2840948905439227 2314 62-63 -1.3814322930048952 2314 64-65 -1.3929559830112233 2314 66-67 -1.288851445939116 2314 68-69 -1.564205965884259 2314 70-71 1.1030267916289311 2314 72-73 0.7649447225463426 2314 74-75 0.6285639147095274 2314 76-77 0.6265924764135669 2314 78-79 -1.6033996175518332 2314 80-81 -0.9680498942050555 2314 82-83 0.24508773590516952 2314 84-85 0.20428001612323854 2314 86-87 -0.07164527014464284 2314 88-89 -0.2602886479819304 2314 90-91 0.13891118071342134 2314 92-93 -0.40864974742495974 2314 94-95 0.3694991193174104 2314 96-97 0.5961723663817509 2314 98-99 0.3046792596614267 2314 100-101 0.40809331658932635 2315 1 -0.22916559040650597 2315 2 -0.1673387368826802 2315 3 -0.3371638552770264 2315 4 -0.1407444578036987 2315 5 -0.09051904194519977 2315 6 -0.010479867336357529 2315 7 -0.7519042136985732 2315 8 -2.6809981060808497 2315 9 -0.6282091590136787 2315 10-11 -1.0854179457498816 2315 12-13 -0.4865140872582785 2315 14-15 -0.3282466289549859 2315 16-17 -0.14314642563212487 2315 18-19 0.012046603347897644 2315 20-21 -0.4176868448953357 2315 22-23 -0.04408537311553573 2315 24-25 -4.630305395779729 2315 26-27 -0.5585544250810344 2315 28-29 -0.47780734947277637 2315 30-31 -0.7013921075348648 2315 32-33 0.11456546871337281 2315 34-35 -1.69969795430206 2315 36-37 -3.239134349996185 2315 38-39 -0.8316768624265549 2315 40-41 -0.07717945949678295 2315 42-43 -1.3842889688859543 2315 44-45 -4.866580017313588 2315 46-47 -3.031811744586605 2315 48-49 -2.3583699205236 2315 50-51 -0.918333767262272 2315 52-53 -0.1594777433915624 2315 54-55 -1.167831552487911 2315 56-57 -1.2164443336859172 2315 58-59 -0.4872378453603474 2315 60-61 -1.1457587251177976 2315 62-63 -0.19215286117855257 2315 64-65 0.18712652662188134 2315 66-67 0.3566314657713896 2315 68-69 3.1685815912562063 2315 70-71 3.8152204469432895 2315 72-73 -1.9346849115153582 2315 74-75 -1.6935030742572792 2315 76-77 -0.06296009281315307 2315 78-79 0.11059949692055682 2315 80-81 0.0021347502675759245 2315 82-83 -0.5648298741276214 2315 84-85 -0.08283854884337316 2315 86-87 0.22164356742086966 2315 88-89 0.10354951586460714 2315 90-91 0.3707414107112683 2315 92-93 -0.616479785957381 2315 94-95 0.3250049470463914 2315 96-97 0.3606366820125082 2315 98-99 -1.6605338652936972 2315 100-101 -3.4459917778270643 2316 1 -0.4873680133794984 2316 2 -0.38841174930784916 2316 3 -0.946858979593383 2316 4 -0.23967907373533137 2316 5 -0.22876426129278116 2316 6 -0.4800511278253481 2316 7 -0.6542754690157935 2316 8 -0.816548188182658 2316 9 -5.093030151217242 2316 10-11 -1.3580473369035815 2316 12-13 -0.7160642727954354 2316 14-15 -0.5056434198733655 2316 16-17 -0.4757996715625836 2316 18-19 -0.5411501953281288 2316 20-21 -3.8790094597408924 2316 22-23 -1.2360840025971953 2316 24-25 -0.3966139175593355 2316 26-27 -0.6809927840792582 2316 28-29 -0.1694575311311084 2316 30-31 -0.0352655673179072 2316 32-33 -1.2815321096065801 2316 34-35 -0.6351455707637896 2316 36-37 -0.4669746199624427 2316 38-39 -5.606954521684671 2316 40-41 -5.206554774651359 2316 42-43 -3.7869304490457623 2316 44-45 -2.5170147690041134 2316 46-47 -2.533670671703476 2316 48-49 -2.7912061177230925 2316 50-51 -1.2581343956320055 2316 52-53 -0.9208207220709532 2316 54-55 -0.21495701956314406 2316 56-57 -3.713227494411516 2316 58-59 -1.0038582893565717 2316 60-61 -0.7410892656967754 2316 62-63 -0.2613937750298412 2316 64-65 0.10134274528420306 2316 66-67 -0.0779961023343212 2316 68-69 2.669772390682141 2316 70-71 3.3416578750234045 2316 72-73 2.097501582414278 2316 74-75 0.6334550861450872 2316 76-77 -1.309357633637788 2316 78-79 0.05720811049903318 2316 80-81 -0.03605551905220494 2316 82-83 0.3373871978763141 2316 84-85 0.43714257420590386 2316 86-87 0.47576546597665725 2316 88-89 0.3512484026416942 2316 90-91 0.2885290579450164 2316 92-93 0.11717258077264248 2316 94-95 0.16378867666639607 2316 96-97 0.60335321744558 2316 98-99 0.6161123728920472 2316 100-101 0.45152997591923594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 26.0 4 104.0 5 225.0 6 609.0 7 1360.0 8 2877.0 9 5057.0 10 7881.0 11 11392.0 12 14538.0 13 17810.0 14 22214.0 15 28416.0 16 34382.0 17 38042.0 18 32810.0 19 26360.0 20 25377.0 21 25633.0 22 26914.0 23 30062.0 24 35604.0 25 43275.0 26 53392.0 27 66222.0 28 80842.0 29 95316.0 30 111573.0 31 128693.0 32 148180.0 33 171725.0 34 197932.0 35 220428.0 36 219733.0 37 178239.0 38 73482.0 39 4463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.417366845305274 20.999673048712992 16.07802442550807 20.50493568047367 2 16.036150799218984 22.37515097989044 38.83872275368978 22.749975467200795 3 18.762986042468597 27.688168099219325 30.815408850589677 22.733437007722397 4 12.746611989182597 18.56030133804113 36.68913638364062 32.00395028913565 5 12.649785737110644 39.46045271430988 32.89154267904946 14.998218869530017 6 29.64245811592615 37.62329801178159 17.213330833179366 15.52091303911289 7 26.970624871916776 35.51551233977276 20.229561814396575 17.284300973913886 8 27.340238098403674 34.22136173728303 19.196276910084777 19.242123254228524 9 27.63099561296333 16.550942852990488 20.34468198454153 35.47337954950465 10-11 24.969325378412037 28.181563497842234 25.65485044173289 21.194260682012843 12-13 26.349590294145557 25.96594808635067 27.243869083013518 20.44059253649025 14-15 23.455035510285857 26.308489046620686 27.0697905142997 23.166684928793753 16-17 20.79835814319621 29.980712354591923 27.530214025469597 21.690715476742273 18-19 20.563718253655335 26.87502636168414 32.261658282887 20.29959710177352 20-21 22.018050622616276 25.852845155348042 32.30457193497705 19.824532287058634 22-23 21.530861265626168 26.097046713341342 32.27239446964213 20.09969755139036 24-25 20.907434334663005 26.511422017118296 32.51082645058162 20.070317197637085 26-27 20.74255903906228 26.66302786252656 32.30772087453255 20.286692223878607 28-29 20.419476222077805 26.41040541268515 32.8007528061169 20.369365559120155 30-31 20.59059436523828 26.501879132630428 32.60959314686806 20.297933355263233 32-33 20.552054772225198 26.784602910765898 32.117155931988144 20.54618638502076 34-35 20.815939818323447 26.733975844619916 32.184639239586474 20.265445097470163 36-37 20.97533174962912 26.47024543438449 31.942766392003964 20.611656423982428 38-39 20.69551525270259 26.779776449885027 32.1054697978338 20.419238499578586 40-41 20.618539435525296 26.64689692164147 32.219468946962834 20.5150946958704 42-43 20.727646482560207 26.715037247258937 31.659550646865704 20.897765623315156 44-45 20.760417524617797 27.052836635933758 31.287182502637805 20.89956333681064 46-47 20.913639063496902 27.31900970241655 30.696421570010724 21.07092966407582 48-49 21.026144548047906 27.566147094483068 30.125016201980486 21.28269215548854 50-51 21.146920679433066 27.725722554889305 29.7430676345237 21.384289131153928 52-53 21.241244946978828 27.887768339966712 29.505859027361087 21.365127685693366 54-55 20.974424862138466 27.96277302821999 29.577411166348483 21.485390943293062 56-57 21.102093151981556 28.174087986399993 29.24886739212882 21.474951469489636 58-59 21.147693023757036 27.662620989144653 29.48699341617285 21.70269257092546 60-61 21.344091814674115 27.934720060158234 29.604088192341287 21.11709993282636 62-63 21.25504406963206 27.863643522077 29.666018002884257 21.215294405406688 64-65 21.11760234234515 27.63738490698568 29.71320851951939 21.531804231149778 66-67 20.94359060073704 28.135523252837253 29.456899993640274 21.463986152785434 68-69 21.098060438622962 27.798317201298385 29.718738549749187 21.384883810329473 70-71 20.69326341669619 27.7448508320715 30.11566029690213 21.446225454330182 72-73 20.99353676589842 28.145354327629263 29.174617744389963 21.686491162082362 74-75 20.86358309117472 28.404092576070678 28.936489843099565 21.79583448965503 76-77 20.247896394787585 28.536841657986578 29.56506160660659 21.650200340619246 78-79 20.744886701802297 28.348163724584825 28.94228115047032 21.964668423142562 80-81 20.498366097351024 28.877404003204767 28.703480195449373 21.920749703994836 82-83 20.829466889178235 28.61307744502925 28.483352894789927 22.074102771002586 84-85 20.575702372528106 28.415802977861006 28.51719228414313 22.49130236546776 86-87 20.603645991529746 28.3746167468036 28.66093195715587 22.360805304510784 88-89 20.676300396503652 28.309677673729094 28.56343817745401 22.450583752313243 90-91 20.55031330478203 28.56027431991978 28.654102992843526 22.235309382454655 92-93 20.612170277051522 28.420527785960477 28.472061592986087 22.495240344001914 94-95 20.33642497138151 28.13315684308809 28.651019598217054 22.87939858731335 96-97 20.539606297822264 28.10792676313042 28.498817535920512 22.853649403126806 98-99 20.36632515690741 28.119096484760448 28.325432939735084 23.18914541859706 100-101 20.165564029852696 28.069471965467603 28.678552997523738 23.086411007155963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6007.0 1 4072.5 2 2244.0 3 2464.5 4 2336.0 5 2393.0 6 3969.5 7 7530.5 8 10849.0 9 12152.5 10 10910.5 11 8688.0 12 7670.0 13 7259.0 14 7119.0 15 6914.0 16 6818.5 17 7018.0 18 7122.0 19 7300.5 20 7646.5 21 8107.0 22 8880.0 23 9935.5 24 11063.5 25 12399.5 26 14164.5 27 16209.5 28 18186.0 29 21173.5 30 25172.0 31 29304.0 32 34878.0 33 41389.5 34 47296.0 35 52566.0 36 59429.0 37 64996.0 38 66675.5 39 66970.5 40 66274.0 41 67312.0 42 68666.0 43 69496.5 44 70859.5 45 71725.0 46 70957.5 47 70143.0 48 73229.5 49 74289.0 50 71163.0 51 67531.0 52 64588.0 53 63193.5 54 61266.5 55 57550.0 56 52767.0 57 47685.5 58 43719.0 59 40126.0 60 35568.0 61 30317.0 62 24588.0 63 18845.5 64 13931.0 65 10436.5 66 7815.5 67 6028.5 68 4435.5 69 2930.0 70 2167.5 71 1750.5 72 1435.5 73 1215.5 74 964.5 75 729.0 76 506.5 77 283.5 78 164.5 79 104.0 80 59.0 81 40.5 82 29.0 83 16.0 84 8.0 85 5.0 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020447469488111814 2 0.020401623143968067 3 5.043097855812331E-4 4 0.09710255689645923 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011140661626930877 12-13 0.0 14-15 0.0 16-17 4.584634414374846E-4 18-19 1.3753903243124538E-4 20-21 0.0 22-23 9.169268828749692E-5 24-25 0.0037823233918592484 26-27 0.003644784359428003 28-29 0.0012149281198093342 30-31 2.9800123693436497E-4 32-33 9.398500549468435E-4 34-35 0.0032092440900623924 36-37 0.08802498075599705 38-39 0.10198519254776844 40-41 0.08135433768308165 42-43 0.08454065860107217 44-45 0.08392173295513156 46-47 0.08222541822181287 48-49 0.07709062767771305 50-51 0.07683847278492242 52-53 0.06028794254902923 54-55 0.05102698103199203 56-57 0.02805796261597406 58-59 0.021845782984496142 60-61 0.013020361736824563 62-63 0.0022235476909718004 64-65 0.006280949147693539 66-67 0.15736757627341658 68-69 0.7143089649316728 70-71 0.8713097704519396 72-73 0.3553779366302662 74-75 0.05290668114188572 76-77 0.19629112245145905 78-79 0.014533291093568263 80-81 0.003278013606278015 82-83 0.00178800742160619 84-85 1.1461586035937115E-4 86-87 0.001673391561246819 88-89 3.8969392522186195E-4 90-91 0.004401249037799852 92-93 0.004882635651309211 94-95 0.07628831665519743 96-97 0.03642492042220816 98-99 0.013524671522405796 100-101 0.01331836297375893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2181199.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.162349781440426 #Duplication Level Percentage of deduplicated Percentage of total 1 70.18344688456108 28.187321426448875 2 13.755585379307947 11.049132629044674 3 5.7756957947893115 6.958965442245687 4 3.0306642658399543 4.868743932591064 5 1.7718743974455462 3.5581319659493507 6 1.1617277753113817 2.79946303577222 7 0.7985561702021551 2.24503245594505 8 0.5623436396102681 1.8068033561116692 9 0.42283716348576383 1.5283920654456624 >10 2.1976834729709847 16.264715374733687 >50 0.20263918566539701 5.6547140361269195 >100 0.13009395006973043 9.544062291906863 >500 0.005109906992941695 1.3954892873642022 >1k 0.0011613424983958397 0.9170797943833331 >5k 2.3226849967916794E-4 0.5697816482650186 >10k+ 3.484027495187519E-4 2.6521712576658065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22935 1.051485902936871 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19795 0.9075283823255007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14132 0.6479005354394533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7174 0.3289016728872515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5042 0.23115726717277976 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4417 0.20250330208293693 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3421 0.1568403433157635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.292317207187423E-5 0.0 16-17 0.0 0.0 0.0 1.3753903243124538E-4 0.0 18-19 0.0 0.0 0.0 1.8338537657499384E-4 0.0 20-21 0.0 0.0 0.0 3.896939252218619E-4 0.0 22-23 0.0 0.0 0.0 8.023110225155981E-4 0.0 24-25 0.0 0.0 0.0 0.0013066208080968313 0.0 26-27 0.0 0.0 0.0 0.0021318550026843035 0.0 28-29 0.0 0.0 0.0 0.004355402693656104 0.0 30-31 0.0 0.0 0.0 0.010384196948559027 0.0 32-33 0.0 0.0 0.0 0.020447469488111814 0.0 34-35 0.0 0.0 0.0 0.03573722526005192 0.0 36-37 0.0 0.0 0.0 0.06248856706792916 0.0 38-39 0.0 0.0 0.0 0.10253534867749343 0.0 40-41 0.0 0.0 0.0 0.15007800755456058 0.0 42-43 0.0 0.0 0.0 0.19649743100010592 0.0 44-45 0.0 0.0 0.0 0.25071073295008844 0.0 46-47 0.0 0.0 0.0 0.31782978077653623 0.0 48-49 0.0 0.0 0.0 0.39645626098306486 0.0 50-51 0.0 0.0 0.0 0.4863838650210274 0.0 52-53 0.0 0.0 0.0 0.5738128433031557 0.0 54-55 0.0 0.0 0.0 0.6726117149329337 0.0 56-57 0.0 0.0 0.0 0.7808320102842519 0.0 58-59 0.0 0.0 0.0 0.8951727925787606 0.0 60-61 0.0 0.0 0.0 1.0157028313326752 0.0 62-63 0.0 0.0 0.0 1.1312126954028496 0.0 64-65 0.0 0.0 0.0 1.2502985743162363 0.0 66-67 0.0 0.0 0.0 1.3815566575997882 0.0 68-69 0.0 0.0 0.0 1.5291589625705861 0.0 70-71 0.0 0.0 0.0 1.720773757919383 0.0 72-73 0.0 0.0 0.0 1.9297872408707322 0.0 74-75 0.0 0.0 0.0 2.1470989121121 0.0 76-77 0.0 0.0 0.0 2.390405460482973 0.0 78-79 0.0 0.0 0.0 2.674744486862501 0.0 80-81 0.0 0.0 0.0 2.9976861350110653 0.0 82-83 0.0 0.0 0.0 3.333006296078441 0.0 84-85 0.0 0.0 0.0 3.653357625782884 0.0 86-87 0.0 0.0 0.0 3.9889070185709787 0.0 88-89 0.0 0.0 0.0 4.351964217845323 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9605 0.0 55.73468 1 GTATCAA 17060 0.0 40.80984 1 TATCAAC 22905 0.0 30.225882 2 ATCAACG 23010 0.0 30.019196 3 TCAACGC 23330 0.0 29.61424 4 CAACGCA 23840 0.0 28.952806 5 AACGCAG 24545 0.0 28.19846 6 TGGTATC 4420 0.0 27.054047 2 GTGGTAT 4670 0.0 26.219028 1 ACGCAGA 28185 0.0 24.422163 7 CGCAGAG 28370 0.0 24.229486 8 GTACATG 17650 0.0 23.877125 1 GAGTACT 16260 0.0 23.11472 12-13 TACATGG 18135 0.0 22.869038 2 GCAGAGT 28930 0.0 22.744509 9 ACATGGG 18360 0.0 21.653378 3 GTACTTT 17555 0.0 21.53108 14-15 AGTACTT 16930 0.0 21.276127 12-13 TATAACG 140 4.8869016E-4 20.336546 2 CAGAGTA 28640 0.0 20.191685 10-11 >>END_MODULE