FastQCFastQC Report
Fri 27 May 2016
SRR939479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755869
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83170.3017922840309173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.24649212281135277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.1610744197202407No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31650.11484580725716644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164600.027.6613981
GTACATG118850.026.242751
GGTATCA117600.025.9247381
TACATGG120850.024.7387712
TATCAAC198400.023.0712572
ATCAACG199750.022.9077993
ACATGGG123750.022.8440763
TCAACGC202000.022.7527314
CAACGCA201650.022.451865
GAGTACT96550.022.25979212-13
AACGCAG205450.021.9565096
GTACTTT104250.020.69957414-15
AGTACTT102200.020.48997912-13
ACGCAGA222450.020.0926387
CGCAGAG223850.019.872958
AGAGTAC163050.019.68396810-11
CATGGGA78050.019.1379474
CATGGGG46700.018.9656854
GCAGAGT226950.018.4365989
ACTTTTT128450.018.27266916-17
Produced by FastQC (version 0.11.5)