FastQCFastQC Report
Fri 27 May 2016
SRR939479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755869
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118310.4293019733521441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111480.40451850214941276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71860.26075259745655543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43610.15824409650821575No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.10356080060409258No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27980.10152877368263875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.028.0081271
GTATCAA178900.025.7275351
GTACATG136700.025.6597541
TACATGG138450.024.8858172
CGAGCGG2653.274181E-1123.2449786
ACATGGG141450.023.0810623
CATTCCG5650.022.6436799
GAGTACT111300.022.01075412-13
ATCAACG215400.021.228593
TCAACGC216400.021.06484
CAACGCA217850.020.9245935
TATCAAC219500.020.9002422
AACGCAG220750.020.7781226
GTACTTT121350.020.1292914-15
CATGGGA86400.020.072754
TATCGCG1200.004833035619.743337
AGTACTT117150.019.39482312-13
GTGTAGC17150.019.3474251
TAGATCG1250.006118399618.9539415
AGAGTAC193300.018.83838510-11
Produced by FastQC (version 0.11.5)