##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755869 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98395460742147 31.0 31.0 31.0 30.0 34.0 2 31.24198755456083 31.0 31.0 34.0 30.0 34.0 3 31.237353807456014 31.0 31.0 34.0 28.0 34.0 4 35.16638309005254 35.0 35.0 37.0 33.0 37.0 5 34.7933410477784 35.0 35.0 37.0 32.0 37.0 6 34.752231328847635 35.0 35.0 37.0 32.0 37.0 7 34.62321467384698 35.0 35.0 37.0 30.0 37.0 8 34.6472183547186 35.0 35.0 37.0 30.0 37.0 9 35.99526211151546 37.0 35.0 39.0 30.0 39.0 10-11 35.85387204544193 37.0 35.0 39.0 30.0 39.0 12-13 35.81896726586061 37.0 35.0 39.0 30.0 39.0 14-15 36.680063348439276 38.0 36.0 40.0 31.0 41.0 16-17 36.68484623180565 38.0 36.0 40.0 31.0 41.0 18-19 36.659268092931846 38.0 35.0 40.0 30.0 41.0 20-21 36.561543745366706 38.0 35.0 40.0 30.0 41.0 22-23 36.42256725555532 38.0 35.0 40.0 30.0 41.0 24-25 36.20281188982495 38.0 34.0 40.0 29.5 41.0 26-27 35.83549853059053 38.0 34.0 40.0 28.0 41.0 28-29 35.57050534695227 38.0 34.0 40.0 27.0 41.0 30-31 35.373446633348685 38.0 34.0 40.0 27.0 41.0 32-33 35.18078780232297 38.0 34.0 40.0 27.0 41.0 34-35 35.14525128008624 38.0 33.5 40.0 27.0 41.0 36-37 35.154750098789165 38.0 33.5 40.0 26.5 41.0 38-39 35.144002853546375 38.0 34.0 40.0 26.5 41.0 40-41 34.95160981889923 38.0 33.0 40.0 25.5 41.0 42-43 34.660050967589534 38.0 33.0 40.0 25.0 41.0 44-45 34.49342022425594 38.0 33.0 40.0 24.5 41.0 46-47 34.187103777429186 37.5 33.0 40.0 24.0 41.0 48-49 33.896677236835274 37.0 33.0 40.0 23.5 41.0 50-51 33.665746630191784 37.0 32.0 40.0 23.0 41.0 52-53 33.32055478689299 37.0 32.0 40.0 22.0 41.0 54-55 33.11109889475878 37.0 31.0 39.5 21.0 41.0 56-57 32.72798180900471 36.0 31.0 39.0 19.5 40.0 58-59 32.30895880754855 36.0 30.5 39.0 18.5 40.0 60-61 32.027636110424694 35.5 30.0 39.0 17.5 40.0 62-63 31.66214957967886 35.0 30.0 39.0 14.0 40.0 64-65 31.23796704415195 35.0 29.0 38.0 12.5 40.0 66-67 29.549994938075795 33.5 26.5 37.5 7.5 39.5 68-69 25.89024188014742 30.5 17.0 36.0 4.5 39.0 70-71 25.278796633657116 29.5 16.0 35.0 4.0 38.0 72-73 27.110632435721726 31.0 22.5 35.0 4.5 38.0 74-75 27.71709195901547 32.0 25.0 35.0 3.5 38.0 76-77 27.756491872436605 31.5 26.0 34.5 2.0 37.0 78-79 28.591113547124337 33.0 27.0 35.0 2.0 37.0 80-81 28.43695763477872 33.0 27.0 35.0 2.0 37.0 82-83 28.02442441930295 33.0 26.0 35.0 2.0 36.0 84-85 27.677103483511008 33.0 26.0 35.0 2.0 36.0 86-87 27.221423986408645 32.0 26.0 35.0 2.0 35.5 88-89 26.727589736667454 32.0 25.0 34.0 2.0 35.0 90-91 26.26651811098423 32.0 24.5 34.0 2.0 35.0 92-93 25.698696672447056 31.5 23.5 34.0 2.0 35.0 94-95 24.317043008938377 31.0 17.5 34.0 2.0 35.0 96-97 23.508649177446387 30.5 7.0 34.0 2.0 35.0 98-99 23.17581296498491 31.0 2.0 34.0 2.0 35.0 100-101 21.896929788752658 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3759590797514747 1101 2 0.2705313486433347 1101 3 0.38784060259251163 1101 4 0.33904987612751114 1101 5 0.4210041606175068 1101 6 0.5792706077347418 1101 7 0.5245870682699874 1101 8 0.5210166996269265 1101 9 0.6424716628202205 1101 10-11 0.7890339209221864 1101 12-13 0.7276484231416234 1101 14-15 0.8233765392638617 1101 16-17 0.7736401569183116 1101 18-19 0.8012021523647803 1101 20-21 0.7461120556230298 1101 22-23 0.8076218310302892 1101 24-25 0.7560928077207762 1101 26-27 0.8583261603841876 1101 28-29 0.7593920430545609 1101 30-31 0.8224783867870045 1101 32-33 0.6616441260465109 1101 34-35 0.8208883890223504 1101 36-37 0.932285805564014 1101 38-39 0.8733392212330315 1101 40-41 1.0822568766880423 1101 42-43 1.040909572561496 1101 44-45 0.7358329821615541 1101 46-47 0.823251128729396 1101 48-49 0.8241052280682126 1101 50-51 0.8363461627937596 1101 52-53 0.9872627909744764 1101 54-55 0.8061096276964506 1101 56-57 0.5783103513338474 1101 58-59 0.773508470699646 1101 60-61 0.497591859884551 1101 62-63 0.4218743831117777 1101 64-65 0.16828199213846773 1101 66-67 -2.845958290547852 1101 68-69 -8.062958458663076 1101 70-71 -11.223892987603389 1101 72-73 -11.10050289287307 1101 74-75 -9.700803573681497 1101 76-77 -4.6448957537078 1101 78-79 -1.4541020162777727 1101 80-81 -0.8054547896010718 1101 82-83 -0.5354931283796276 1101 84-85 -0.6029748206634622 1101 86-87 -0.536623071601138 1101 88-89 -0.4075005435793848 1101 90-91 -0.5618031531834227 1101 92-93 -1.2104645115643216 1101 94-95 -4.788271067661995 1101 96-97 -1.6632443003189685 1101 98-99 -1.149165443978422 1101 100-101 -0.8105173728203283 1102 1 0.370931897483608 1102 2 0.2866874846383425 1102 3 0.3539204790449979 1102 4 0.24571484925774456 1102 5 0.45432953029735756 1102 6 0.49588107520119706 1102 7 0.47224570374332586 1102 8 0.6103245371724313 1102 9 0.7076587028310257 1102 10-11 0.6959887105557314 1102 12-13 0.689294359107798 1102 14-15 0.5535397336768071 1102 16-17 0.6345998074046548 1102 18-19 0.7472468879361998 1102 20-21 0.8389924486041238 1102 22-23 0.8212205253943381 1102 24-25 0.9201974964981687 1102 26-27 0.8956666226994727 1102 28-29 0.8513152855316761 1102 30-31 0.5763682282901357 1102 32-33 0.473695855520198 1102 34-35 0.6954250606385841 1102 36-37 0.9288180880983177 1102 38-39 0.904298680188468 1102 40-41 0.9161728672002099 1102 42-43 0.9711051256170649 1102 44-45 0.8071021365122775 1102 46-47 0.8612407800121034 1102 48-49 0.8724950075758144 1102 50-51 0.9487858935617908 1102 52-53 0.9158832543381337 1102 54-55 0.8487282785729064 1102 56-57 0.8036167251799995 1102 58-59 0.8038661193855958 1102 60-61 0.5560496127028287 1102 62-63 0.5665437251627914 1102 64-65 0.6871210729110828 1102 66-67 -2.638531326826829 1102 68-69 -7.795105006016065 1102 70-71 -11.283876939329536 1102 72-73 -9.452292749194651 1102 74-75 -7.914429082372465 1102 76-77 -4.005091296682799 1102 78-79 -1.1241083771199882 1102 80-81 -0.3842596770665878 1102 82-83 -0.16073115593387044 1102 84-85 -0.15952633693300555 1102 86-87 -0.10929040156957726 1102 88-89 0.011867464912491243 1102 90-91 -0.15226320034034657 1102 92-93 -0.4766566856114771 1102 94-95 -4.121912362531891 1102 96-97 -0.9284430337956486 1102 98-99 -0.30824647868886146 1102 100-101 -0.012795179327738992 1103 1 0.3969497610772308 1103 2 0.24080347390584933 1103 3 0.2816460007099124 1103 4 0.1911829619985781 1103 5 0.4067948330656961 1103 6 0.3589171139948135 1103 7 0.4059849526340855 1103 8 0.35473859310163647 1103 9 0.5359669008361934 1103 10-11 0.6574747144659696 1103 12-13 0.5826609488380328 1103 14-15 0.7476145247212997 1103 16-17 0.6944228811671209 1103 18-19 0.5783485129772217 1103 20-21 0.6716341260942897 1103 22-23 0.6521717521031434 1103 24-25 0.8055840952181015 1103 26-27 0.714098994465175 1103 28-29 0.7837762864775186 1103 30-31 0.8272490264436598 1103 32-33 0.8692671844248565 1103 34-35 0.9393525694145737 1103 36-37 0.8716417933462637 1103 38-39 0.8518724247319085 1103 40-41 0.9933188837401659 1103 42-43 0.9318851342758663 1103 44-45 0.7069497527235313 1103 46-47 0.5993636963168356 1103 48-49 0.5265682543763361 1103 50-51 0.5677725386090486 1103 52-53 0.8224781136602743 1103 54-55 0.6018211510597169 1103 56-57 0.5628316977282992 1103 58-59 0.6262387950916022 1103 60-61 0.32993534416431913 1103 62-63 0.3453345269838941 1103 64-65 0.29047103444286293 1103 66-67 -3.035888573881042 1103 68-69 -7.965551430401888 1103 70-71 -11.645353181411839 1103 72-73 -9.508885871242317 1103 74-75 -7.6532340581471345 1103 76-77 -3.8994646783289113 1103 78-79 -1.0180949808035606 1103 80-81 -0.3452931623424931 1103 82-83 -0.1732629163444166 1103 84-85 -0.29899511700784487 1103 86-87 -0.18812102282443988 1103 88-89 -0.0224149166426173 1103 90-91 -0.06804746945795159 1103 92-93 0.004378729969392481 1103 94-95 0.941123676261725 1103 96-97 1.1039213967505965 1103 98-99 0.5709588974862108 1103 100-101 0.5160680640809545 1104 1 0.410212263882066 1104 2 0.20701147234865758 1104 3 0.3187574128057129 1104 4 0.19877880624711253 1104 5 0.3936777761552577 1104 6 0.4362175516842228 1104 7 0.4166084505267449 1104 8 0.4028750202691995 1104 9 0.5505244668613258 1104 10-11 0.6186452565357925 1104 12-13 0.6213009486217516 1104 14-15 0.7463825601473673 1104 16-17 0.724719531315678 1104 18-19 0.7648755732129047 1104 20-21 0.7756304277674602 1104 22-23 0.5499420503212917 1104 24-25 0.7344139045974671 1104 26-27 0.8757530224783849 1104 28-29 0.8680412034679321 1104 30-31 0.7139081234355231 1104 32-33 0.5507344577468913 1104 34-35 0.718753754068679 1104 36-37 0.8412264074415319 1104 38-39 0.8268024728263796 1104 40-41 0.9200282109312425 1104 42-43 1.0076906152611116 1104 44-45 0.959659993682493 1104 46-47 1.024768390055904 1104 48-49 0.851399328972775 1104 50-51 0.8985274244286074 1104 52-53 0.9561300762336344 1104 54-55 0.9551626488280149 1104 56-57 1.030677773798125 1104 58-59 1.1205005490393276 1104 60-61 0.9715393582547307 1104 62-63 1.046992934594659 1104 64-65 0.8863595655745016 1104 66-67 -2.5718265782444227 1104 68-69 -7.265898337784211 1104 70-71 -8.83266354078215 1104 72-73 -8.137367421511716 1104 74-75 -6.874673756225253 1104 76-77 -3.337849471933616 1104 78-79 -0.38520198626724067 1104 80-81 0.39536112790587197 1104 82-83 0.620444196716786 1104 84-85 0.6940698172846744 1104 86-87 0.8912264712076805 1104 88-89 0.7568759670358141 1104 90-91 0.7956917876866072 1104 92-93 0.9054864978074662 1104 94-95 1.703114987356436 1104 96-97 2.0848302984178204 1104 98-99 1.8158922167615792 1104 100-101 1.6525242126844866 1105 1 0.4682144510982802 1105 2 0.34304979814982417 1105 3 0.4826780320048236 1105 4 0.33792730019824546 1105 5 0.4892579112564377 1105 6 0.5206651614846791 1105 7 0.6182773487260533 1105 8 0.5703012965308858 1105 9 0.7456077703704622 1105 10-11 0.8195028065220669 1105 12-13 0.8607152002868403 1105 14-15 1.0383109131592647 1105 16-17 0.9710904329534671 1105 18-19 1.0026272545012844 1105 20-21 1.1238585778447785 1105 22-23 1.1299178724045902 1105 24-25 1.163767720139056 1105 26-27 1.2452345300127732 1105 28-29 1.3387553036661828 1105 30-31 1.2487658660736614 1105 32-33 1.2581948847470628 1105 34-35 1.2975446805345072 1105 36-37 1.3366744925871572 1105 38-39 1.2476246715215282 1105 40-41 1.2282921522133918 1105 42-43 1.3835514131323592 1105 44-45 1.1375433760293703 1105 46-47 1.0268358218906855 1105 48-49 0.8950258192752258 1105 50-51 0.9558904365501775 1105 52-53 1.0783529449628446 1105 54-55 1.0789763581640344 1105 56-57 1.3665722499882307 1105 58-59 1.3351785403413814 1105 60-61 1.3723096731386661 1105 62-63 1.3681399001165921 1105 64-65 1.3369807703030716 1105 66-67 2.169468900407143 1105 68-69 -4.858562744421363 1105 70-71 -5.174039764183757 1105 72-73 -1.3328382499786393 1105 74-75 1.3868003793958081 1105 76-77 1.4320384465085496 1105 78-79 1.5189876282372552 1105 80-81 1.5881794176536168 1105 82-83 1.6896451671707275 1105 84-85 1.6370164242227645 1105 86-87 1.645605048147555 1105 88-89 1.503320494623221 1105 90-91 1.5249188950817825 1105 92-93 1.556950787379897 1105 94-95 2.2771678482961377 1105 96-97 2.311949395732256 1105 98-99 2.0689966692841075 1105 100-101 2.0154171999942747 1106 1 0.08867145204689919 1106 2 0.11748697959577825 1106 3 0.07547307439776674 1106 4 -0.006933086636266239 1106 5 0.2376392361637869 1106 6 0.11721565148778268 1106 7 0.1810049647495262 1106 8 0.30274809074345654 1106 9 0.33504634352581775 1106 10-11 0.3715591872278594 1106 12-13 0.2865047894334154 1106 14-15 0.2787290372910647 1106 16-17 0.2241709448597362 1106 18-19 0.24224341151038686 1106 20-21 0.20797061088508428 1106 22-23 0.30284356422723135 1106 24-25 0.35804661306674745 1106 26-27 0.46396572459368457 1106 28-29 0.5345435618009589 1106 30-31 0.43297876552895076 1106 32-33 0.4607890787608895 1106 34-35 0.5717510484938018 1106 36-37 0.6414146287925817 1106 38-39 0.5402691838509455 1106 40-41 0.5865009723559709 1106 42-43 0.5440145315325822 1106 44-45 0.5263582054083997 1106 46-47 0.3998006094750721 1106 48-49 0.2702923467998559 1106 50-51 0.29739917743903277 1106 52-53 0.6446547286920676 1106 54-55 0.5632915547635804 1106 56-57 0.5454465240014983 1106 58-59 0.7536132596322958 1106 60-61 0.5539607000554554 1106 62-63 0.6252302476363987 1106 64-65 0.5625696563112363 1106 66-67 1.2877806745288005 1106 68-69 -5.473102921034055 1106 70-71 -6.048262834823635 1106 72-73 -2.2165440751279135 1106 74-75 0.43744640786471933 1106 76-77 0.6789577720194089 1106 78-79 0.5536478040175226 1106 80-81 0.5970189401195363 1106 82-83 0.6636587335192843 1106 84-85 0.6161235494280497 1106 86-87 0.6991450517720708 1106 88-89 0.6198979430345908 1106 90-91 0.5928039108371479 1106 92-93 0.6456763804388679 1106 94-95 1.6375112189902943 1106 96-97 1.6980732075766127 1106 98-99 1.290834714196869 1106 100-101 1.1831117878488584 1107 1 0.020501596762869667 1107 2 0.040845239354631246 1107 3 -0.08165025701724815 1107 4 -0.05117252068593103 1107 5 0.13854178097520276 1107 6 0.1716411739952548 1107 7 0.09679932349354914 1107 8 0.07743384934430253 1107 9 0.15421629706511197 1107 10-11 0.3297923645224685 1107 12-13 0.24495860243960266 1107 14-15 0.07131918649870528 1107 16-17 0.012794914933984103 1107 18-19 0.24829926962977567 1107 20-21 0.22281768357994025 1107 22-23 0.11773458860858454 1107 24-25 0.37415038817447765 1107 26-27 0.399444473383042 1107 28-29 0.35804727444641316 1107 30-31 0.21056744090758173 1107 32-33 0.2545767140373343 1107 34-35 0.44829813031236654 1107 36-37 0.3934683393325642 1107 38-39 0.37182982840548817 1107 40-41 0.19820361331719738 1107 42-43 0.3191617389775203 1107 44-45 0.3345161121501121 1107 46-47 0.1164868102246217 1107 48-49 0.3768703614819984 1107 50-51 0.41863521115462987 1107 52-53 0.4804557021210414 1107 54-55 0.4212354931154181 1107 56-57 0.37023238015579096 1107 58-59 0.44233311631997196 1107 60-61 0.46825929781242337 1107 62-63 0.3631141450017523 1107 64-65 0.4059161078924447 1107 66-67 1.1579196706407018 1107 68-69 -5.646882274795125 1107 70-71 -6.124927384323232 1107 72-73 -2.3896667854292737 1107 74-75 0.2753809242382559 1107 76-77 0.28346233738021454 1107 78-79 0.17549573995508894 1107 80-81 0.34121837023898394 1107 82-83 0.6121146344498989 1107 84-85 0.48750915924235017 1107 86-87 0.3987466793003911 1107 88-89 0.3615497544406274 1107 90-91 0.2283825817241123 1107 92-93 0.38621053818068063 1107 94-95 1.1054563153506365 1107 96-97 1.4017318126325478 1107 98-99 1.0583425746182407 1107 100-101 0.9565526873056456 1108 1 0.07303919241650902 1108 2 0.036245431105783155 1108 3 0.12351015702319756 1108 4 0.07220425256833352 1108 5 0.10672751326929131 1108 6 0.24476627386565042 1108 7 0.11059608502968388 1108 8 0.13017562446281516 1108 9 0.1460402411021846 1108 10-11 0.2025803463416196 1108 12-13 0.1282309778457389 1108 14-15 0.10068085934994286 1108 16-17 0.052358681068604085 1108 18-19 0.11949021293890638 1108 20-21 0.2546268467992334 1108 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2316 100-101 0.6568395653070027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 20.0 4 67.0 5 161.0 6 403.0 7 1037.0 8 2358.0 9 3854.0 10 6093.0 11 9312.0 12 12316.0 13 16044.0 14 19226.0 15 24373.0 16 29221.0 17 33150.0 18 30862.0 19 27023.0 20 26281.0 21 27144.0 22 28804.0 23 33102.0 24 40201.0 25 50576.0 26 64071.0 27 80009.0 28 98335.0 29 118317.0 30 138191.0 31 161982.0 32 188341.0 33 217156.0 34 253147.0 35 292801.0 36 316746.0 37 277534.0 38 120111.0 39 7489.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.95919785075338 21.05979316187076 15.31370885141467 22.66730013596119 2 15.652216202052212 21.615305692490328 39.31977119563383 23.412706909823626 3 19.245726121234355 25.433066666086084 30.722723032190576 24.598484180488985 4 12.92042546289392 17.831689387267684 37.53251696651764 31.71536818332076 5 12.925011593451933 38.52397032366596 34.66521957163374 13.885798511248371 6 30.83880257007862 37.52576773424281 17.301983512278703 14.333446183399865 7 27.567384371318088 33.5613920690715 21.013190394753885 17.85803316485653 8 25.836496582384722 35.96346560740006 19.610946674170652 18.589091136044566 9 25.761347872485956 17.03513483405779 21.054883232838716 36.14863406061754 10-11 24.615193973298442 27.46796019694695 27.330489947091095 20.58635588266351 12-13 25.511081985391904 24.858765057410203 28.045346132200045 21.58480682499785 14-15 22.419951746617855 26.194568754900903 26.74383288900887 24.641646609472367 16-17 21.265697482016478 29.554084103088112 27.422241945878156 21.757976469017247 18-19 21.058623653900653 27.95929579187128 30.067406116408357 20.91467443781971 20-21 22.03376576817177 26.87690411877492 30.120219836363717 20.969110276689594 22-23 21.621056733828787 27.083653105426997 29.877817124108585 21.41747303663563 24-25 21.566446010314714 27.19370913494074 30.14403079391655 21.095814060828 26-27 21.479172729509287 27.269093690603796 30.178877140984767 21.07285643890215 28-29 21.01214898153609 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28.646095561244973 21.614076892085308 54-55 21.57959079046165 27.69126508430071 28.78991920667725 21.939224918560388 56-57 21.718735962524992 27.949246842674107 28.62564101633361 21.706376178467295 58-59 21.93128897766732 27.678988787206617 28.622473347114635 21.76724888801143 60-61 21.81833324472626 27.831426890183902 28.891318678267965 21.458921186821872 62-63 21.709371222192207 27.657594515160582 29.028822513271486 21.60421174937573 64-65 21.521803107777938 27.766044653850468 28.849822424612565 21.862329813759036 66-67 21.154387830322094 27.767789503372224 29.114275292794705 21.963547373510973 68-69 21.18818454582782 27.489156547177597 29.508878234174684 21.813780672819902 70-71 20.907699686242637 27.469603611321624 29.862543521477917 21.76015318095782 72-73 21.726788602576626 27.5190172351369 28.876959716756456 21.877234445530018 74-75 21.216498513063904 28.01710299024329 28.741683396628282 22.024715100064522 76-77 21.425670710131648 27.94523630227093 28.464729311643183 22.164363675954238 78-79 21.448189681247523 27.928587020008283 28.478605736212742 22.144617562531447 80-81 21.371124155831716 28.030487488474385 28.35812148765939 22.24026686803451 82-83 21.466822261870938 28.030994942067277 28.380957149995155 22.12122564606663 84-85 21.411594673041424 27.99998838841759 28.21340564446278 22.375011294078202 86-87 21.345767432155995 27.955585592532284 28.411921857494704 22.286725117817017 88-89 21.453759423596658 27.849667325628992 28.150863182584818 22.545710068189535 90-91 21.280275843499748 27.903789346372175 28.395522042786297 22.42041276734178 92-93 21.497277943669054 27.939387209836923 28.297139037695118 22.2661958087989 94-95 20.985017481665526 27.6891107443691 28.617199165884166 22.70867260808121 96-97 20.81861463816286 27.733767671980974 28.696773118913477 22.750844570942686 98-99 21.126855125470247 27.7156670685334 28.047824076728723 23.10965372926763 100-101 20.897604663842788 27.594439459767916 28.400940969837617 23.10701490655168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1547.0 1 1086.0 2 665.5 3 749.5 4 747.0 5 763.0 6 1198.0 7 2252.0 8 3357.5 9 4117.5 10 4134.5 11 3648.5 12 3477.5 13 3559.0 14 3764.0 15 3826.5 16 3978.0 17 4282.0 18 4569.0 19 5036.0 20 5700.0 21 6647.5 22 7920.0 23 9440.0 24 11241.0 25 13923.0 26 17793.5 27 22253.0 28 26268.0 29 30232.0 30 35316.5 31 41649.5 32 48474.0 33 55808.5 34 64211.0 35 71336.0 36 78653.0 37 84926.5 38 87953.0 39 90456.0 40 92426.0 41 94565.5 42 96704.0 43 98511.0 44 100495.0 45 102028.5 46 102699.0 47 105261.5 48 110703.0 49 109039.0 50 99789.5 51 91605.5 52 85935.0 53 82776.5 54 78743.0 55 72278.0 56 65444.5 57 58240.5 58 51061.5 59 43919.5 60 36240.0 61 28659.0 62 21781.0 63 16302.5 64 12194.0 65 9104.5 66 6649.0 67 4834.5 68 3404.5 69 2324.5 70 1701.5 71 1234.0 72 891.5 73 685.0 74 536.5 75 381.0 76 228.0 77 120.0 78 70.0 79 46.5 80 28.0 81 17.0 82 10.5 83 5.5 84 4.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024348036862419802 2 0.018215669903032403 3 0.0 4 0.0 5 1.0885858507788288E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.7172053533749245E-4 18-19 0.0013063030209345945 20-21 3.628619502596096E-5 22-23 0.0 24-25 0.0 26-27 3.9914814528557057E-4 28-29 0.01792538034282471 30-31 0.004590203670784061 32-33 0.008527255831100826 34-35 0.009452553804262829 36-37 0.09555969460086819 38-39 0.09742843364470517 40-41 0.1120880564351934 42-43 0.0978820110825297 44-45 0.09327366431423263 46-47 0.09423524848242061 48-49 0.09661199425662105 50-51 0.0941445329948557 52-53 0.07338882944000603 54-55 0.06099709383864037 56-57 0.03507060749259126 58-59 0.016147356786552627 60-61 0.009343695219184945 62-63 0.001977597628914872 64-65 0.005007494913582612 66-67 0.41874269059958946 68-69 0.801072184490627 70-71 0.8875240441399791 72-73 0.2795488464800032 74-75 0.14766667065814812 76-77 0.014296760840228618 78-79 0.008545398928613807 80-81 0.0018143097512980481 82-83 0.0 84-85 0.0 86-87 2.3586026766874623E-4 88-89 7.620100955451801E-4 90-91 0.0037556211851869593 92-93 0.001741737361246126 94-95 0.22247066170416663 96-97 0.22671614652220407 98-99 0.014333047035254579 100-101 0.007311668297731133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2755869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.47393891310546 #Duplication Level Percentage of deduplicated Percentage of total 1 70.58871908769478 34.21713257011278 2 14.921782221137766 14.466351197241902 3 5.894187399610378 8.571434398533281 4 2.913562409906852 5.6492738510938025 5 1.626732676873831 3.9427070203367305 6 0.9989806375164797 2.905471583900939 7 0.6455102731511088 2.1903297883956205 8 0.43674351851412413 1.6936542909718715 9 0.31192814553052783 1.3608347284552564 >10 1.4848628156331434 12.72874808232847 >50 0.10879533149119097 3.6610125190952485 >100 0.06426044085633076 5.685670593346069 >500 0.002648586017778064 0.8661013790249587 >1k 0.0010594344070961675 0.9547620470550439 >5k 7.567388622115482E-5 0.2635976667487915 >10k+ 1.5134777244230963E-4 0.8429182833593766 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11831 0.4293019733521441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11148 0.40451850214941276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7186 0.26075259745655543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4361 0.15824409650821575 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2854 0.10356080060409258 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2798 0.10152877368263875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.628619502596096E-5 0.0 0.0 0.0 0.0 7 3.628619502596096E-5 0.0 0.0 0.0 0.0 8 3.628619502596096E-5 0.0 0.0 0.0 0.0 9 3.628619502596096E-5 0.0 0.0 0.0 0.0 10-11 3.628619502596096E-5 0.0 0.0 0.0 0.0 12-13 3.628619502596096E-5 0.0 0.0 7.257239005192192E-5 0.0 14-15 3.628619502596096E-5 0.0 0.0 7.257239005192192E-5 0.0 16-17 3.628619502596096E-5 0.0 0.0 2.3586026766874623E-4 0.0 18-19 5.442929253894144E-5 0.0 0.0 2.9028956020768764E-4 0.0 20-21 7.257239005192192E-5 0.0 0.0 5.442929253894144E-4 0.0 22-23 7.257239005192192E-5 0.0 0.0 9.07154875649024E-4 0.0 24-25 7.257239005192192E-5 0.0 0.0 0.0014695908985514188 0.0 26-27 7.257239005192192E-5 0.0 0.0 0.0020320269214538136 0.0 28-29 9.071548756490241E-5 0.0 0.0 0.0034471885274662906 0.0 30-31 1.0885858507788288E-4 0.0 0.0 0.006331941032030187 0.0 32-33 1.0885858507788288E-4 0.0 0.0 0.013444035257118535 0.0 34-35 1.0885858507788288E-4 0.0 0.0 0.026216775906256793 0.0 36-37 1.0885858507788288E-4 0.0 0.0 0.048986363285047296 0.0 38-39 1.814309751298048E-4 0.0 0.0 0.08360339333981404 0.0 40-41 1.814309751298048E-4 0.0 0.0 0.11820228029706781 0.0 42-43 1.9957407264278528E-4 0.0 0.0 0.1556859197588855 0.0 44-45 2.1771717015576576E-4 0.0 0.0 0.20543429313947797 0.0 46-47 2.721464626947072E-4 0.0 0.0 0.26305677084070395 0.0 48-49 2.902895602076877E-4 0.0 0.0 0.32750105320681067 0.0 50-51 2.902895602076877E-4 0.0 0.0 0.3963359651710585 0.0 52-53 2.902895602076877E-4 0.0 0.0 0.4635198552616253 0.0 54-55 2.902895602076877E-4 0.0 0.0 0.5404465887166625 0.0 56-57 2.902895602076877E-4 0.0 0.0 0.6299102025531693 0.0 58-59 2.902895602076877E-4 1.814309751298048E-5 0.0 0.7163802053000342 0.0 60-61 2.902895602076877E-4 3.628619502596096E-5 0.0 0.8051180952360217 0.0 62-63 2.902895602076877E-4 3.628619502596096E-5 0.0 0.8951622881929439 0.0 64-65 2.902895602076877E-4 3.628619502596096E-5 0.0 0.9926632942277009 0.0 66-67 2.902895602076877E-4 3.628619502596096E-5 0.0 1.0943553557879566 0.0 68-69 2.902895602076877E-4 3.628619502596096E-5 0.0 1.2167305485130098 0.0 70-71 2.902895602076877E-4 3.628619502596096E-5 0.0 1.3745210675834012 0.0 72-73 3.2657575523364863E-4 3.628619502596096E-5 0.0 1.5517247009926813 0.0 74-75 3.628619502596096E-4 3.628619502596096E-5 0.0 1.739759763617211 0.0 76-77 3.628619502596096E-4 3.628619502596096E-5 0.0 1.9527234422245758 0.0 78-79 3.628619502596096E-4 3.628619502596096E-5 0.0 2.203243332683811 0.0 80-81 4.354343403115315E-4 3.628619502596096E-5 0.0 2.475680084938725 0.0 82-83 4.354343403115315E-4 3.628619502596096E-5 0.0 2.759510702431792 0.0 84-85 4.354343403115315E-4 3.628619502596096E-5 0.0 3.040311422640191 0.0 86-87 4.354343403115315E-4 3.628619502596096E-5 0.0 3.3266639306875616 0.0 88-89 4.354343403115315E-4 3.628619502596096E-5 0.0 3.651171372804731 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12930 0.0 28.008127 1 GTATCAA 17890 0.0 25.727535 1 GTACATG 13670 0.0 25.659754 1 TACATGG 13845 0.0 24.885817 2 CGAGCGG 265 3.274181E-11 23.244978 6 ACATGGG 14145 0.0 23.081062 3 CATTCCG 565 0.0 22.643679 9 GAGTACT 11130 0.0 22.010754 12-13 ATCAACG 21540 0.0 21.22859 3 TCAACGC 21640 0.0 21.0648 4 CAACGCA 21785 0.0 20.924593 5 TATCAAC 21950 0.0 20.900242 2 AACGCAG 22075 0.0 20.778122 6 GTACTTT 12135 0.0 20.12929 14-15 CATGGGA 8640 0.0 20.07275 4 TATCGCG 120 0.0048330356 19.74333 7 AGTACTT 11715 0.0 19.394823 12-13 GTGTAGC 1715 0.0 19.347425 1 TAGATCG 125 0.0061183996 18.953941 5 AGAGTAC 19330 0.0 18.838385 10-11 >>END_MODULE