FastQCFastQC Report
Fri 27 May 2016
SRR939478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737030
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81790.29882756126165955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.23083415234761767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.15666616734197286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27750.10138727014318441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162100.027.8546181
GGTATCA117200.027.2175031
GTACATG112950.025.1185381
TACATGG117850.023.6292022
TATCAAC191600.023.218532
ATCAACG194700.023.0587223
TCAACGC195850.022.9332964
GAGTACT90700.022.82561112-13
CAACGCA194900.022.797585
AACGCAG200150.022.0316456
ACATGGG121550.022.0109793
GTACTTT103050.020.60212314-15
ACGCAGA217550.019.9350387
CGCAGAG219350.019.5713068
CATGGGA76050.019.4357624
AGTACTT98300.019.29967512-13
AGAGTAC156000.019.07727610-11
CATGGGG45550.018.9465144
GCAGAGT219350.018.2223229
ACTTTTT130300.018.04342816-17
Produced by FastQC (version 0.11.5)