FastQCFastQC Report
Fri 27 May 2016
SRR939478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737030
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116490.42560731888214604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.3924692093254367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70260.2567016072165815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43430.15867564476823418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124100.028.4575311
GTATCAA170850.025.6438221
GTACATG132500.023.0351541
TACATGG133650.022.3348182
ATCAACG205550.021.1661263
TCAACGC206950.020.9587614
TATCAAC208850.020.8597412
GAGTACT109600.020.73856212-13
CAACGCA209250.020.732115
ACATGGG137750.020.7000963
AACGCAG213650.020.3495276
CATTCCG5000.019.9130219
GTACTTT117600.019.30762114-15
AGTACTT116750.018.75796312-13
GAGTACA115150.018.4675851
AGTACAT103800.018.3335972
AGAGTAC189800.018.19606810-11
ACGCAGA238350.018.1412647
CGCAGAG238550.018.0863048
CATGGGA85850.018.0755834
Produced by FastQC (version 0.11.5)