##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2737030 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.886263212314077 31.0 31.0 31.0 28.0 34.0 2 31.130501675173456 31.0 31.0 34.0 28.0 34.0 3 31.136758091800235 31.0 31.0 34.0 28.0 34.0 4 35.04040912960399 35.0 35.0 37.0 32.0 37.0 5 34.61364800531963 35.0 35.0 37.0 30.0 37.0 6 34.68225229537126 35.0 35.0 37.0 31.0 37.0 7 34.52837162910162 35.0 35.0 37.0 30.0 37.0 8 34.426843695538594 35.0 35.0 37.0 30.0 37.0 9 35.92440893961703 37.0 35.0 39.0 30.0 39.0 10-11 35.727560713620235 37.0 35.0 39.0 30.0 39.0 12-13 35.713553925240134 37.0 35.0 39.0 30.0 39.0 14-15 36.54351815654195 38.0 35.0 40.0 30.5 41.0 16-17 36.459889917172994 38.0 35.0 40.0 30.0 41.0 18-19 36.470338834430024 38.0 35.0 40.0 30.0 41.0 20-21 36.35181784635171 38.0 34.5 40.0 29.5 41.0 22-23 36.18358567498347 38.0 34.0 40.0 29.0 41.0 24-25 36.02828960588667 38.0 34.0 40.0 29.0 41.0 26-27 35.56389663248119 38.0 34.0 40.0 27.0 41.0 28-29 35.37410112421128 38.0 34.0 40.0 27.0 41.0 30-31 35.09264330314246 37.5 33.0 40.0 27.0 41.0 32-33 34.870048008242506 37.0 33.0 40.0 26.0 41.0 34-35 35.12166582025041 38.0 33.0 40.0 27.0 41.0 36-37 34.97126337672587 38.0 33.0 40.0 26.0 41.0 38-39 34.80506333507488 38.0 33.0 40.0 25.0 41.0 40-41 34.66984559906176 38.0 33.0 40.0 25.0 41.0 42-43 34.52119852540893 37.0 33.0 40.0 25.0 41.0 44-45 34.14460948546417 37.0 33.0 40.0 24.0 41.0 46-47 33.764780619868986 37.0 32.0 40.0 23.0 41.0 48-49 33.393421884305255 37.0 31.5 40.0 22.5 41.0 50-51 33.17059568218105 37.0 31.0 39.5 21.5 41.0 52-53 33.081450879237714 37.0 31.0 39.0 21.5 40.0 54-55 32.75475223143334 36.0 31.0 39.0 19.5 40.0 56-57 32.26244907801522 36.0 30.5 39.0 17.5 40.0 58-59 31.80706039027705 35.0 30.0 39.0 15.0 40.0 60-61 31.447984676821225 35.0 29.0 38.5 13.0 40.0 62-63 31.31322053466714 35.0 29.0 38.0 13.0 40.0 64-65 30.870016039283456 34.5 28.5 38.0 10.0 40.0 66-67 30.009192445826315 34.0 27.0 37.5 7.5 39.5 68-69 26.56951129508993 31.0 19.5 36.0 3.5 39.0 70-71 25.251363887133135 30.0 13.5 35.0 2.0 38.0 72-73 26.26206216227078 31.0 20.5 35.0 2.0 38.0 74-75 27.006716769637162 31.0 23.0 35.0 2.0 38.0 76-77 26.66221122895986 30.5 23.5 34.5 2.0 36.5 78-79 27.773786001614887 32.0 26.0 35.0 2.0 37.0 80-81 27.778506264089177 32.0 26.0 35.0 2.0 37.0 82-83 27.4448566511876 32.0 26.0 35.0 2.0 36.0 84-85 27.070003799739133 32.0 25.5 35.0 2.0 36.0 86-87 26.618459607676932 32.0 25.0 34.0 2.0 35.5 88-89 26.130365578747767 31.5 24.5 34.0 2.0 35.0 90-91 25.522663617132437 31.0 22.0 34.0 2.0 35.0 92-93 24.95333902076338 31.0 20.0 34.0 2.0 35.0 94-95 24.099532157119214 31.0 16.5 34.0 2.0 35.0 96-97 23.417373576467924 30.5 4.5 34.0 2.0 35.0 98-99 22.55297110371461 30.0 2.0 34.0 2.0 35.0 100-101 21.11840663054479 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2207803951381493 1101 2 0.13536425194783064 1101 3 0.3495473482998541 1101 4 0.31321340202578796 1101 5 0.4575405993825825 1101 6 0.26165016627100357 1101 7 0.43691610317668506 1101 8 0.6595005246215138 1101 9 0.6217487173866445 1101 10-11 0.6919640844485713 1101 12-13 0.6244954135982113 1101 14-15 0.6957826080506138 1101 16-17 0.7925067650088948 1101 18-19 0.560356891570251 1101 20-21 0.6396520210960333 1101 22-23 0.7009965749275935 1101 24-25 0.6749498293826122 1101 26-27 0.7650179033011639 1101 28-29 0.6954269172780201 1101 30-31 0.49973636459011317 1101 32-33 0.9709541801750845 1101 34-35 0.8554494944857183 1101 36-37 0.9287814296639425 1101 38-39 0.94666622150271 1101 40-41 0.9297984044668652 1101 42-43 0.5335765495261171 1101 44-45 0.7994052534538341 1101 46-47 0.6958992277092548 1101 48-49 0.7746115071344803 1101 50-51 0.5112606030954296 1101 52-53 0.5105511009085717 1101 54-55 0.6599607698405734 1101 56-57 0.7268470568415282 1101 58-59 0.7303612692668899 1101 60-61 0.6273114146821932 1101 62-63 0.4066619876161255 1101 64-65 0.30534434726611437 1101 66-67 -0.00647147911898216 1101 68-69 -7.351821413293532 1101 70-71 -7.6954431292978 1101 72-73 -4.9715105093923135 1101 74-75 -2.8531092316508335 1101 76-77 -0.2357341297958655 1101 78-79 0.18632904932521654 1101 80-81 0.058263296117733177 1101 82-83 -0.17479624638164637 1101 84-85 -0.1951045203993118 1101 86-87 -0.1733504023052923 1101 88-89 -0.023541566628338728 1101 90-91 -0.3171227696799157 1101 92-93 -0.1404759310363275 1101 94-95 -0.22018828556099024 1101 96-97 -0.3685279360365321 1101 98-99 -0.6213077427871916 1101 100-101 -0.5506267627801904 1102 1 0.3678603630807089 1102 2 0.34529932207148306 1102 3 0.38685635184174316 1102 4 0.31967698125857424 1102 5 0.47909581827398995 1102 6 0.44481150794614166 1102 7 0.5558326246916039 1102 8 0.6745714188181751 1102 9 0.7676391268621643 1102 10-11 0.6167699348512272 1102 12-13 0.5444215779914074 1102 14-15 0.8153707923929616 1102 16-17 0.7250763195844456 1102 18-19 0.6044015590575427 1102 20-21 0.6081477003976303 1102 22-23 0.7003197257346798 1102 24-25 0.5322650497239252 1102 26-27 0.5578317208730894 1102 28-29 0.5059253862743276 1102 30-31 0.4996499631606426 1102 32-33 0.8325047233585323 1102 34-35 0.6512268924872942 1102 36-37 0.7290729034044574 1102 38-39 0.9001711957971423 1102 40-41 0.6610265825490984 1102 42-43 0.4338692999104481 1102 44-45 0.6239841918173639 1102 46-47 0.6088378759691508 1102 48-49 0.818469009519923 1102 50-51 0.7056368617151989 1102 52-53 0.5114105557611452 1102 54-55 0.1968488798754109 1102 56-57 0.4032965006636715 1102 58-59 0.4854103100571372 1102 60-61 0.5863175839494303 1102 62-63 0.5049965601678501 1102 64-65 0.3886392008071411 1102 66-67 0.385876158286365 1102 68-69 -6.867265383847286 1102 70-71 -7.532404543764432 1102 72-73 -1.4188022178301232 1102 74-75 0.2757694430743385 1102 76-77 0.5731290382664262 1102 78-79 0.3667317574970923 1102 80-81 0.11942963790514938 1102 82-83 -0.039775832049176785 1102 84-85 -0.136778369137307 1102 86-87 -0.03686777486289472 1102 88-89 -0.18735993076203528 1102 90-91 -0.2745852258081065 1102 92-93 -0.23419857082112827 1102 94-95 -0.07336125267384475 1102 96-97 -0.26691711930707385 1102 98-99 -0.0304764162733413 1102 100-101 0.11765911378909522 1103 1 0.35760058771912995 1103 2 0.2786249544245081 1103 3 0.28775890748366706 1103 4 0.3385715651486265 1103 5 0.4329532614308036 1103 6 0.47472216795127054 1103 7 0.3535919093687667 1103 8 0.5244490935825326 1103 9 0.5287588793930027 1103 10-11 0.580775575388131 1103 12-13 0.4870797749294695 1103 14-15 0.562515796415731 1103 16-17 0.5724440521545162 1103 18-19 0.49963658311598635 1103 20-21 0.5336485893659599 1103 22-23 0.5918856675906241 1103 24-25 0.6974238576976575 1103 26-27 0.7163385915235025 1103 28-29 0.7803863560827011 1103 30-31 0.6022296640889024 1103 32-33 0.7072158100594592 1103 34-35 0.6629156421086648 1103 36-37 0.7820784621700057 1103 38-39 0.9266819329845433 1103 40-41 0.792181382038855 1103 42-43 0.4808739428817148 1103 44-45 0.5485312690085138 1103 46-47 0.7298996484416236 1103 48-49 0.6987455541972878 1103 50-51 0.5077996524154926 1103 52-53 0.4829444481916454 1103 54-55 0.3141869621715969 1103 56-57 0.4634969271120184 1103 58-59 0.6702588075167775 1103 60-61 0.5262490377261742 1103 62-63 0.11758397369912998 1103 64-65 0.26127107906386726 1103 66-67 0.3402372770490949 1103 68-69 -6.733838420240719 1103 70-71 -7.873907121249548 1103 72-73 -0.9335208382943527 1103 74-75 0.6475185955259661 1103 76-77 0.8296246033228591 1103 78-79 0.5977732580949713 1103 80-81 0.5766886348078444 1103 82-83 0.33592472189275924 1103 84-85 -0.028323558838433627 1103 86-87 0.20010535520205153 1103 88-89 0.27531475992119425 1103 90-91 0.024022760138468158 1103 92-93 0.06520536430482338 1103 94-95 0.45562467106642757 1103 96-97 0.4937777483194843 1103 98-99 0.6113104601089425 1103 100-101 0.6965405850167699 1104 1 0.292964155116163 1104 2 0.24946817725031778 1104 3 0.15028908012599018 1104 4 0.26677555741896697 1104 5 0.370271904375052 1104 6 0.2649272307859363 1104 7 0.39538501028967943 1104 8 0.5496536517101944 1104 9 0.4655756343210484 1104 10-11 0.48289857579157314 1104 12-13 0.31074821311229783 1104 14-15 0.544956166991021 1104 16-17 0.5221448221606764 1104 18-19 0.4781714647668025 1104 20-21 0.47855328756472204 1104 22-23 0.5727822635885573 1104 24-25 0.50080898340223 1104 26-27 0.7932814722436206 1104 28-29 0.6078212312606937 1104 30-31 0.5294555764728557 1104 32-33 0.6532459343995356 1104 34-35 0.39627193933956306 1104 36-37 0.7237925013623752 1104 38-39 0.6894550588925199 1104 40-41 0.8279047171644152 1104 42-43 0.8110285436899218 1104 44-45 0.8876987659209092 1104 46-47 0.735224551270619 1104 48-49 0.9283864272460747 1104 50-51 0.8084627756161495 1104 52-53 0.47909474267272856 1104 54-55 0.5664615318885566 1104 56-57 0.6322116416684906 1104 58-59 0.6525433206664211 1104 60-61 0.8515101490595498 1104 62-63 0.40687476332218253 1104 64-65 0.519029390655767 1104 66-67 0.24940232252747663 1104 68-69 -6.598207385237902 1104 70-71 -4.292950274836713 1104 72-73 0.40404921487498413 1104 74-75 1.056879777409904 1104 76-77 1.3504130564940162 1104 78-79 0.963861967479474 1104 80-81 0.6241235576917603 1104 82-83 0.4767429630772213 1104 84-85 0.5435562595277581 1104 86-87 0.4158093759333106 1104 88-89 0.46229997639713005 1104 90-91 0.41358224254219067 1104 92-93 0.48157151074921956 1104 94-95 0.7312277634078619 1104 96-97 0.3754986593335161 1104 98-99 0.4894586310258262 1104 100-101 0.6608015286204498 1105 1 0.19208722397821987 1105 2 0.22532925547234228 1105 3 0.10994829003279705 1105 4 0.1762433867296096 1105 5 0.3987535838902403 1105 6 0.3107432603724547 1105 7 0.22063042526065857 1105 8 0.3670762510247485 1105 9 0.38427800901524733 1105 10-11 0.2664975335383204 1105 12-13 0.29366116315089386 1105 14-15 0.4414390194337443 1105 16-17 0.4246041297827574 1105 18-19 0.5374517429218741 1105 20-21 0.5542508400681285 1105 22-23 0.5162746725293559 1105 24-25 0.6038314769553708 1105 26-27 0.7544330345101642 1105 28-29 0.6254340763231383 1105 30-31 0.4962377072273796 1105 32-33 0.5069732978071002 1105 34-35 0.42182700115544236 1105 36-37 0.5104772244868272 1105 38-39 0.49603937322418545 1105 40-41 0.42936321769961694 1105 42-43 0.5066280861793402 1105 44-45 0.5105219951784434 1105 46-47 0.6415681594448372 1105 48-49 0.7360330886354305 1105 50-51 0.4304138928070316 1105 52-53 0.44792298871257685 1105 54-55 0.3776047629704422 1105 56-57 0.641493624785177 1105 58-59 0.6743705941789493 1105 60-61 0.8307513540123921 1105 62-63 0.5107930878085405 1105 64-65 0.5368594166324989 1105 66-67 0.6046742090443686 1105 68-69 -2.96594673630047 1105 70-71 -2.923534575399607 1105 72-73 0.9961785096974829 1105 74-75 1.3757180327038618 1105 76-77 1.2605662050740527 1105 78-79 0.9236028757044537 1105 80-81 0.6067108439274911 1105 82-83 0.43805415708066064 1105 84-85 0.3923868333511429 1105 86-87 0.5364398201796128 1105 88-89 0.5916892440886876 1105 90-91 0.4499112633445499 1105 92-93 0.3804099532124674 1105 94-95 0.5136063021040869 1105 96-97 0.5691813301574946 1105 98-99 0.5956688649476369 1105 100-101 0.8211484350617297 1106 1 0.08181362552562632 1106 2 0.1348001472704965 1106 3 0.07257696351697973 1106 4 0.1776632245527736 1106 5 0.26322296557060554 1106 6 0.2469587198353338 1106 7 0.2665173986684124 1106 8 0.41935075009632783 1106 9 0.3978623090238642 1106 10-11 0.4994822132025476 1106 12-13 0.27215735841730293 1106 14-15 0.35786424897710134 1106 16-17 0.4428702310729591 1106 18-19 0.31643619143449087 1106 20-21 0.35838452856081204 1106 22-23 0.37890154695625 1106 24-25 0.3828929578303075 1106 26-27 0.46573007849547565 1106 28-29 0.37429298334676986 1106 30-31 0.39371564696877925 1106 32-33 0.5246826726401395 1106 34-35 0.3159162256745205 1106 36-37 0.42077442875179827 1106 38-39 0.42169414830385676 1106 40-41 0.23433379493533124 1106 42-43 0.17071824838888716 1106 44-45 0.25575836404842534 1106 46-47 0.39309730362783313 1106 48-49 0.42646570194605005 1106 50-51 0.17166769399702986 1106 52-53 0.07318164408624739 1106 54-55 0.06589595293235817 1106 56-57 0.1216277799424148 1106 58-59 0.5462157291464074 1106 60-61 0.6322963626097078 1106 62-63 0.1665263017862344 1106 64-65 0.13700710288127027 1106 66-67 0.4277703430337958 1106 68-69 -2.9136205007024714 1106 70-71 -3.4380503509514035 1106 72-73 0.5706552293509297 1106 74-75 1.0848288404587798 1106 76-77 1.1581956472463553 1106 78-79 0.7698463895577419 1106 80-81 0.6004675699485134 1106 82-83 0.5885555488968066 1106 84-85 0.6219497060422192 1106 86-87 0.6572129895842984 1106 88-89 0.5199588671698265 1106 90-91 0.6082230715933896 1106 92-93 0.3857401238394189 1106 94-95 0.4037945162071672 1106 96-97 0.569783959834087 1106 98-99 0.7280820440781355 1106 100-101 0.7774259456461863 1107 1 0.09633860657890381 1107 2 0.1002484232904699 1107 3 0.056660571797213066 1107 4 -0.10097746545137198 1107 5 0.13524157571688988 1107 6 0.11025931903258623 1107 7 0.05707471851427215 1107 8 -0.023286358920024952 1107 9 0.1857370935120457 1107 10-11 0.0033394453554151937 1107 12-13 0.20585745297919544 1107 14-15 -0.039605819780611284 1107 16-17 -0.10817624621044075 1107 18-19 0.016629780000968708 1107 20-21 0.10893026285793184 1107 22-23 0.10296697554141332 1107 24-25 0.03824188662497363 1107 26-27 0.5508790535407542 1107 28-29 0.30460659453483885 1107 30-31 0.2627849793801502 1107 32-33 0.13905607991339508 1107 34-35 0.10923495877415945 1107 36-37 0.13253856895983063 1107 38-39 0.2628267387272416 1107 40-41 0.13070999098296454 1107 42-43 -0.1793419689889788 1107 44-45 0.012234823175027998 1107 46-47 0.27801548355186156 1107 48-49 0.19234294092203896 1107 50-51 0.20596217758149749 1107 52-53 -0.08526344247336937 1107 54-55 -0.1414157647282721 1107 56-57 0.20070304250621973 1107 58-59 0.2747562476569563 1107 60-61 0.3388847848457175 1107 62-63 0.15680852209025176 1107 64-65 -0.07505354494781358 1107 66-67 0.1497298806192866 1107 68-69 -2.9949827643263625 1107 70-71 -3.009625344483183 1107 72-73 0.932777353856654 1107 74-75 1.0210876011714092 1107 76-77 0.6074133937426431 1107 78-79 0.47279990311581344 1107 80-81 0.3423416583879195 1107 82-83 0.29692439063958176 1107 84-85 0.15861716644871748 1107 86-87 0.26273249612235006 1107 88-89 0.31715143226985276 1107 90-91 0.24804850988660831 1107 92-93 0.3460054695398327 1107 94-95 0.6155923140370554 1107 96-97 0.3490956271227681 1107 98-99 0.331659253975765 1107 100-101 0.7163215635154074 1108 1 0.01033492367963973 1108 2 0.044247045394968154 1108 3 0.04357741222897715 1108 4 0.0446616322406328 1108 5 -0.13610553301220563 1108 6 -0.12078190896571073 1108 7 0.0869367038026354 1108 8 0.15207080640352189 1108 9 0.16054558437524946 1108 10-11 0.19267745663159275 1108 12-13 0.09457544609853841 1108 14-15 0.09622145953268557 1108 16-17 0.21017680655000248 1108 18-19 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1.117873910492257 2311 66-67 1.3146474194457554 2311 68-69 3.5793574515778097 2311 70-71 5.120179790999671 2311 72-73 3.984548313011455 2311 74-75 2.6904011751008845 2311 76-77 2.4096453603239745 2311 78-79 1.7245126535263324 2311 80-81 1.400890402929484 2311 82-83 1.345245370702905 2311 84-85 1.34010132186825 2311 86-87 1.0845008180349218 2311 88-89 1.2268752066443085 2311 90-91 1.3622550860905243 2311 92-93 1.3065042434407488 2311 94-95 1.8030545584904694 2311 96-97 1.77947891604067 2311 98-99 1.942745287811146 2311 100-101 1.9057104518376704 2312 1 -0.17145088746206127 2312 2 -0.11404864095107214 2312 3 -0.12771013430113598 2312 4 -0.06860079261965524 2312 5 -1.4952185118994876 2312 6 -0.2806522522297854 2312 7 -0.09165479470547666 2312 8 0.04890015200061271 2312 9 -2.826020844836222 2312 10-11 -0.2413018577673256 2312 12-13 -1.280430084690117 2312 14-15 -4.133363383668325 2312 16-17 -1.0788243586028017 2312 18-19 -0.12585477196127925 2312 20-21 0.1438023642529771 2312 22-23 0.2166354633920733 2312 24-25 0.32860771857718163 2312 26-27 0.2633130533276571 2312 28-29 0.16911854247496194 2312 30-31 0.0050843642917257625 2312 32-33 0.4795769927349127 2312 34-35 0.38725498625512955 2312 36-37 0.7181568507647995 2312 38-39 0.5104487910051319 2312 40-41 0.28753536610560104 2312 42-43 -0.2695860396708767 2312 44-45 0.34397161805132015 2312 46-47 0.32351274931230734 2312 48-49 0.4935331773556939 2312 50-51 0.6318149837876845 2312 52-53 0.4502730492623215 2312 54-55 0.5989050765269255 2312 56-57 0.610812030237085 2312 58-59 0.6453674990881275 2312 60-61 0.7203757168029057 2312 62-63 0.7832841153300087 2312 64-65 0.8610464237034279 2312 66-67 1.226829184726217 2312 68-69 4.1887714368069915 2312 70-71 5.064917712014168 2312 72-73 2.9319985621201603 2312 74-75 2.1883178063425355 2312 76-77 2.121010838788397 2312 78-79 1.4749472003789847 2312 80-81 1.339442360563055 2312 82-83 1.3410374457019145 2312 84-85 1.2596459167666723 2312 86-87 1.2368061835549504 2312 88-89 1.2043148565884785 2312 90-91 1.2741329519742486 2312 92-93 1.6667230850144144 2312 94-95 1.8469809160242825 2312 96-97 1.5443818892858125 2312 98-99 1.9018424317047256 2312 100-101 1.9045035451877794 2313 1 -0.3702421598256329 2313 2 -0.197145244470061 2313 3 -0.1979286359197765 2313 4 0.053332076723243915 2313 5 0.10398829526631914 2313 6 0.01895411047580353 2313 7 -2.9124621617372988 2313 8 -3.0880709787985303 2313 9 -1.0517364346781477 2313 10-11 -0.09827636196632028 2313 12-13 0.05880355432058337 2313 14-15 -0.5602937673481776 2313 16-17 -0.35028077220530207 2313 18-19 0.026924547026226264 2313 20-21 -2.443370245811593 2313 22-23 -0.6362854126265987 2313 24-25 -0.03928243435028378 2313 26-27 -1.560527044560125 2313 28-29 -1.7278446208863656 2313 30-31 -0.8290841814178336 2313 32-33 -1.0326377108497695 2313 34-35 -1.3786573260625161 2313 36-37 -2.1309774578953693 2313 38-39 -1.7973384362377303 2313 40-41 -0.8765683304304446 2313 42-43 0.01961678747493778 2313 44-45 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2314 66-67 -1.0314604547509845 2314 68-69 -1.2431692616725663 2314 70-71 1.7256763471913033 2314 72-73 1.2785447024866023 2314 74-75 0.856204943892827 2314 76-77 0.9906504926478057 2314 78-79 -1.3320860031113 2314 80-81 -0.6675539369322898 2314 82-83 0.28458682696004445 2314 84-85 0.5347480419144794 2314 86-87 0.12209833809313508 2314 88-89 -0.013167639946544796 2314 90-91 0.314617599502796 2314 92-93 -0.21258711083314097 2314 94-95 0.3443522759737121 2314 96-97 0.5918066688387391 2314 98-99 0.279827122437478 2314 100-101 0.33073763979846404 2315 1 -0.4000335505541095 2315 2 -0.3913748250440108 2315 3 -0.5010029552722415 2315 4 -0.10523230885439006 2315 5 -0.13831413298974837 2315 6 -0.17009997340940686 2315 7 -0.9857542883009529 2315 8 -2.7889002650384107 2315 9 -0.7324027334272287 2315 10-11 -1.345163043349693 2315 12-13 -0.8000803676808843 2315 14-15 -0.5947455701443474 2315 16-17 -0.36734387007138025 2315 18-19 -0.1774693087500765 2315 20-21 -0.8752795541265286 2315 22-23 -0.31100469150813126 2315 24-25 -5.244797702930121 2315 26-27 -1.0293655287396675 2315 28-29 -0.8752079395029924 2315 30-31 -1.202276970233143 2315 32-33 -0.27279536353557177 2315 34-35 -2.1458519544657477 2315 36-37 -3.788073116467846 2315 38-39 -1.4092574943687097 2315 40-41 -0.36777473219645174 2315 42-43 -1.8440340587355095 2315 44-45 -5.161229454334741 2315 46-47 -3.6113523087752704 2315 48-49 -2.8435689972049225 2315 50-51 -1.461578522318952 2315 52-53 -0.41693278928400446 2315 54-55 -1.3660058558531745 2315 56-57 -1.4614501085773313 2315 58-59 -0.8852711663870032 2315 60-61 -1.8499221682134745 2315 62-63 -0.7177978030494998 2315 64-65 -0.10543734156317441 2315 66-67 -0.057290565050369224 2315 68-69 2.559687731117389 2315 70-71 3.117307345615597 2315 72-73 -2.8668855606084627 2315 74-75 -2.4379389705415875 2315 76-77 -0.7780812076816623 2315 78-79 -0.8051082062340633 2315 80-81 -0.8307417323770707 2315 82-83 -1.322836846821172 2315 84-85 -0.7975777948806311 2315 86-87 -0.47765375091034556 2315 88-89 -0.36508579875855673 2315 90-91 -0.29627157587799857 2315 92-93 -1.6675009245179062 2315 94-95 -0.6552907551106593 2315 96-97 -0.6011647297192546 2315 98-99 -3.0658537255785667 2315 100-101 -4.39712111969158 2316 1 -0.5213730000953909 2316 2 -0.45078155287275834 2316 3 -0.8045177361081208 2316 4 -0.328600300699442 2316 5 -0.23619792503868098 2316 6 -0.5991947746326503 2316 7 -0.5747593851409079 2316 8 -0.979809541287139 2316 9 -5.198618839441501 2316 10-11 -1.4715432676106488 2316 12-13 -0.7172281760397041 2316 14-15 -0.2641594335490325 2316 16-17 -0.3562786305199026 2316 18-19 -0.44830519050339035 2316 20-21 -3.7624976988767784 2316 22-23 -1.283018962660016 2316 24-25 -0.4625846550198318 2316 26-27 -0.46850824025852233 2316 28-29 0.07197044989558066 2316 30-31 0.04715829989937248 2316 32-33 -1.2490318569096743 2316 34-35 -0.3514890594606541 2316 36-37 -0.33467148547905623 2316 38-39 -5.359632621789707 2316 40-41 -5.22347503800685 2316 42-43 -3.4908220301932076 2316 44-45 -2.1683089650381078 2316 46-47 -2.3188079662676273 2316 48-49 -2.7875129319653738 2316 50-51 -1.1933053316971396 2316 52-53 -0.8638950726682992 2316 54-55 0.04049057635885589 2316 56-57 -3.7619628506772287 2316 58-59 -0.8200244793329752 2316 60-61 -0.41162451275985745 2316 62-63 -0.27125753801382047 2316 64-65 0.018429121229893042 2316 66-67 0.15177671323709419 2316 68-69 2.9311423692417513 2316 70-71 3.976888385370952 2316 72-73 2.7624029205332263 2316 74-75 1.28894889897931 2316 76-77 -1.2165460394859444 2316 78-79 0.2679223748056927 2316 80-81 0.2703683593660493 2316 82-83 0.5529868025162372 2316 84-85 0.4973253651601439 2316 86-87 0.4964400309449033 2316 88-89 0.1190620096002597 2316 90-91 0.1858222059772494 2316 92-93 0.17670906528841357 2316 94-95 0.5140089390789448 2316 96-97 0.6324489298118401 2316 98-99 0.7496386291614954 2316 100-101 0.6965914185347231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 23.0 4 68.0 5 182.0 6 476.0 7 1096.0 8 2638.0 9 4177.0 10 6632.0 11 9888.0 12 13186.0 13 17112.0 14 20773.0 15 26047.0 16 30838.0 17 34821.0 18 32887.0 19 29386.0 20 29332.0 21 30285.0 22 32150.0 23 36391.0 24 43449.0 25 52943.0 26 66917.0 27 83476.0 28 103100.0 29 122652.0 30 143386.0 31 166608.0 32 192143.0 33 221641.0 34 254605.0 35 285108.0 36 293846.0 37 242133.0 38 100941.0 39 5690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.79539757088334 21.138818360017368 15.445749776719477 22.620034292379813 2 15.673149060712468 21.67646206507682 39.29041921966432 23.359969654546394 3 19.230127039487794 25.5755249407842 30.75476530522396 24.439582714504045 4 12.852716279685511 17.917414682329984 37.66510133456112 31.564767703423385 5 12.916884360054512 38.653211692966465 34.555485325334395 13.87441862164463 6 30.761884232178677 37.571784014059034 17.40043039352875 14.265901360233538 7 27.478251973854874 33.59232452695075 21.044855189749473 17.88456830944491 8 25.873154477663746 35.929602525365084 19.577279021421027 18.619963975550142 9 25.720763016846725 17.094843680924214 21.08383905181894 36.10055425041011 10-11 24.592031983004368 27.530658692108958 27.333322736642824 20.543986588243847 12-13 25.467148697675945 24.923347570176432 28.062315721786025 21.547188010361594 14-15 22.370361304041243 26.223059301505646 26.80776242861788 24.59881696583523 16-17 21.283696207961206 29.566857708437727 27.359248347786757 21.790197735814314 18-19 21.020453945101746 27.979409775643425 30.090824825622835 20.909311453631986 20-21 22.02984622017296 26.93362878740825 30.098519197816607 20.938005794602176 22-23 21.645139952112245 27.069678378955548 29.899885843226524 21.38529582570568 24-25 21.490197009777415 27.26904695789428 30.16936921795303 21.071386814375284 26-27 21.503206314490292 27.27101002834184 30.138168752509642 21.087614904658224 28-29 21.078782945274774 27.54695741799144 30.244892128282014 21.129367508451768 30-31 21.35092980483196 27.16086730633907 30.170995818088684 21.317207070740285 32-33 21.304771327606414 27.276676497324093 30.035465631183744 21.38308654388575 34-35 21.746424345525206 27.259703468810955 29.90887837961027 21.08499380605357 36-37 21.616988412470416 26.984667951894636 30.079783110466224 21.318560525168724 38-39 21.297970995729653 27.501191794746887 30.05913898566793 21.141698223855535 40-41 21.499428030683223 27.305030861844497 30.022383315791235 21.173157791681042 42-43 21.386691093053383 27.368193955749113 29.658990455243128 21.58612449595438 44-45 21.533894450164986 27.397885902506374 29.52898656712925 21.539233080199388 46-47 21.779232349559415 27.626790082219138 29.176779286283534 21.417198281937917 48-49 21.70185701104532 27.66998926309512 29.001690884270865 21.626462841588694 50-51 21.45261709983349 27.859954061720515 29.032140707736158 21.655288130709835 52-53 21.70119848741413 28.053211027955932 28.64174350965546 21.603846974974484 54-55 21.586420874518726 27.73247349214954 28.78293628063614 21.8981693526956 56-57 21.731777578603165 27.958706899938512 28.618577874989608 21.690937646468715 58-59 21.945038642734367 27.721961611430434 28.585793046262925 21.74720669957227 60-61 21.771093325070236 27.82804475007998 28.941279085807164 21.459582839042625 62-63 21.682052657337458 27.699021487164867 28.98895654100837 21.62996931448931 64-65 21.454345356964666 27.81884186782123 28.829512360302267 21.897300414911832 66-67 21.348681345783284 27.890943633450938 28.738864238296703 22.021510782469075 68-69 21.48482497374068 27.47737761151219 29.22844054133665 21.809356873410483 70-71 21.139314357783068 27.376946950942422 29.660381656009825 21.823357035264685 72-73 21.673421341178255 27.523304956183985 28.92219813919671 21.88107556344105 74-75 21.48483265529334 27.999737532904344 28.497291800169688 22.01813801163263 76-77 20.867105599356904 28.024879766950317 28.90103075725773 22.206983876435054 78-79 21.48462036826925 27.945757551507523 28.4113103701292 22.158311710094026 80-81 21.39329740268234 28.015659099453806 28.376865542125714 22.214177955738144 82-83 21.45535417913864 28.03600843926101 28.37110534114465 22.137532040455692 84-85 21.323975232209456 28.026094049281657 28.245036348806046 22.404894369702845 86-87 21.350237717908083 27.930269413629162 28.38339948026325 22.33609338819951 88-89 21.41096886394692 27.86218222738599 28.173524490138913 22.55332441852818 90-91 21.29447923818098 27.898976332180336 28.35096201098825 22.455582418650433 92-93 21.4443138836762 27.97146930555312 28.304618489743184 22.279598321027493 94-95 21.211291886847125 27.711850850419882 28.341327015171085 22.735530247561904 96-97 21.114366232393973 27.654351806385197 28.419547954600656 22.811734006620174 98-99 21.114851069450864 27.69931538847607 28.009985889741934 23.175847652331132 100-101 20.916789382716953 27.569363496512185 28.409015336156717 23.104831784614145 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1560.0 1 1127.5 2 688.5 3 735.0 4 709.5 5 740.0 6 1210.5 7 2238.5 8 3342.5 9 3952.0 10 3877.0 11 3491.0 12 3387.0 13 3468.0 14 3642.0 15 3787.5 16 3991.0 17 4279.0 18 4582.0 19 5073.0 20 5702.5 21 6605.0 22 7740.0 23 9214.5 24 11165.0 25 14061.0 26 18010.0 27 22413.0 28 26278.0 29 30120.0 30 35254.5 31 41237.0 32 48058.5 33 55314.5 34 63267.0 35 70577.5 36 78204.0 37 84969.5 38 87607.5 39 89329.5 40 91682.0 41 94106.0 42 96191.5 43 97997.5 44 99696.0 45 101059.5 46 101539.0 47 104466.5 48 110105.0 49 107983.5 50 99014.5 51 91425.0 52 85756.0 53 81829.0 54 77558.0 55 71621.0 56 64852.0 57 57710.0 58 50905.0 59 43886.0 60 36256.5 61 28524.0 62 21671.0 63 16389.0 64 12234.0 65 8976.5 66 6516.0 67 4823.5 68 3508.5 69 2476.5 70 1766.5 71 1273.0 72 906.5 73 668.0 74 505.0 75 366.5 76 230.5 77 130.0 78 80.0 79 45.0 80 26.0 81 13.5 82 10.5 83 11.0 84 6.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020533205701070138 2 0.019765950683770364 3 2.5575167243325797E-4 4 0.09400700759582467 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011928988721351246 12-13 0.0 14-15 0.0 16-17 7.124510874926471E-4 18-19 1.096078596142534E-4 20-21 0.0 22-23 1.644117894213801E-4 24-25 0.0040189548525226245 26-27 0.003178627928813349 28-29 0.001059542642937783 30-31 1.4614381281900454E-4 32-33 5.115033448665159E-4 34-35 0.00305075209259672 36-37 0.0864440652824412 38-39 0.09895762925506844 40-41 0.07966664596295985 42-43 0.08339331318984447 44-45 0.08205975089787104 46-47 0.08054350883987388 48-49 0.07564769111043722 50-51 0.07511791978896833 52-53 0.0577998779699163 54-55 0.04862935371552376 56-57 0.026945265488503962 58-59 0.022524415150729074 60-61 0.01331735494313179 62-63 0.0025757847009349553 64-65 0.006704347413071833 66-67 0.15228185295740274 68-69 0.7096560870724837 70-71 0.8676923526596347 72-73 0.3529190399812936 74-75 0.052940596193684394 76-77 0.19188682623135297 78-79 0.014285557703057694 80-81 0.0033247717416323534 82-83 0.0022104251688874438 84-85 2.740196490356335E-4 86-87 0.0024661768413207015 88-89 3.6535953204751135E-4 90-91 0.003909346992908372 92-93 0.004311242478160633 94-95 0.07517272371877545 96-97 0.03651768522814876 98-99 0.013463498755950792 100-101 0.01315294315371041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2737030.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.52945220317008 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3596638223181 35.34405058521789 2 14.759850435180358 14.620946133104093 3 5.6764012258340015 8.434471296028837 4 2.8006227321731796 5.548532390091324 5 1.551244368040464 3.8416141891148468 6 0.9612205339697872 2.856523589637732 7 0.6210341273568408 2.1531636089220663 8 0.4080243922317579 1.61673797062163 9 0.2907604322452483 1.296108443831769 >10 1.4013962517862895 12.34879687557512 >50 0.10662182883724547 3.693601042238305 >100 0.05920727064924515 5.336842303934916 >500 0.002684770897968869 0.8785230318679671 >1k 0.0010440775714173447 0.9434962076501405 >5k 7.45769693869532E-5 0.25952332752417057 >10k+ 1.491539387739064E-4 0.8270690046390127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11649 0.42560731888214604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10742 0.3924692093254367 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7026 0.2567016072165815 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4343 0.15867564476823418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6535953204751135E-5 0.0 5 0.0 0.0 0.0 3.6535953204751135E-5 0.0 6 0.0 0.0 0.0 3.6535953204751135E-5 0.0 7 0.0 0.0 0.0 3.6535953204751135E-5 0.0 8 0.0 0.0 0.0 3.6535953204751135E-5 0.0 9 0.0 0.0 0.0 3.6535953204751135E-5 0.0 10-11 0.0 0.0 0.0 3.6535953204751135E-5 0.0 12-13 0.0 0.0 0.0 3.6535953204751135E-5 0.0 14-15 3.6535953204751135E-5 0.0 0.0 9.133988301187784E-5 0.0 16-17 3.6535953204751135E-5 0.0 0.0 2.740196490356335E-4 0.0 18-19 3.6535953204751135E-5 0.0 0.0 2.922876256380091E-4 0.0 20-21 3.6535953204751135E-5 0.0 0.0 3.8362750864988696E-4 0.0 22-23 3.6535953204751135E-5 0.0 0.0 8.03790970504525E-4 0.0 24-25 7.307190640950227E-5 0.0 0.0 0.0013335622919734165 0.0 26-27 7.307190640950227E-5 0.0 0.0 0.00177199373043043 0.0 28-29 9.133988301187784E-5 0.0 0.0 0.002831536373368213 0.0 30-31 1.096078596142534E-4 0.0 0.0 0.00630245192781957 0.0 32-33 1.096078596142534E-4 0.0 0.0 0.014687453188309958 0.0 34-35 1.096078596142534E-4 0.0 0.0 0.028023076108044123 0.0 36-37 1.096078596142534E-4 0.0 0.0 0.052100269269975116 0.0 38-39 1.096078596142534E-4 0.0 0.0 0.08589602598436992 0.0 40-41 1.096078596142534E-4 0.0 0.0 0.12274253479136144 0.0 42-43 1.096078596142534E-4 0.0 0.0 0.16064858624129075 0.0 44-45 1.096078596142534E-4 0.0 0.0 0.20681176311549382 0.0 46-47 1.096078596142534E-4 0.0 0.0 0.266310562909431 0.0 48-49 1.096078596142534E-4 0.0 0.0 0.33097920008184056 0.0 50-51 1.096078596142534E-4 0.0 0.0 0.3982418899317874 0.0 52-53 1.096078596142534E-4 0.0 0.0 0.4691947110554141 0.0 54-55 1.096078596142534E-4 0.0 0.0 0.5460846245748128 0.0 56-57 1.096078596142534E-4 0.0 0.0 0.6378081350953406 0.0 58-59 1.096078596142534E-4 0.0 0.0 0.7301162208671443 0.0 60-61 1.096078596142534E-4 0.0 0.0 0.826114437912628 0.0 62-63 1.096078596142534E-4 3.6535953204751135E-5 0.0 0.9194820663273695 0.0 64-65 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.0217279313708656 0.0 66-67 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.1334183403177898 0.0 68-69 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.2582068884886173 0.0 70-71 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.4159143304969255 0.0 72-73 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.5880169380679057 0.0 74-75 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.7747521948973888 0.0 76-77 1.096078596142534E-4 3.6535953204751135E-5 0.0 1.9879942857769186 0.0 78-79 1.096078596142534E-4 3.6535953204751135E-5 0.0 2.2376992579547905 0.0 80-81 1.8267976602375568E-4 3.6535953204751135E-5 0.0 2.5150619467086583 0.0 82-83 1.8267976602375568E-4 3.6535953204751135E-5 0.0 2.7985444076243224 0.0 84-85 2.740196490356335E-4 3.6535953204751135E-5 0.0 3.0822278162826127 0.0 86-87 3.6535953204751135E-4 3.6535953204751135E-5 0.0 3.378260377124109 0.0 88-89 4.018954852522625E-4 3.6535953204751135E-5 0.0 3.7073579756159045 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12410 0.0 28.457531 1 GTATCAA 17085 0.0 25.643822 1 GTACATG 13250 0.0 23.035154 1 TACATGG 13365 0.0 22.334818 2 ATCAACG 20555 0.0 21.166126 3 TCAACGC 20695 0.0 20.958761 4 TATCAAC 20885 0.0 20.859741 2 GAGTACT 10960 0.0 20.738562 12-13 CAACGCA 20925 0.0 20.73211 5 ACATGGG 13775 0.0 20.700096 3 AACGCAG 21365 0.0 20.349527 6 CATTCCG 500 0.0 19.913021 9 GTACTTT 11760 0.0 19.307621 14-15 AGTACTT 11675 0.0 18.757963 12-13 GAGTACA 11515 0.0 18.467585 1 AGTACAT 10380 0.0 18.333597 2 AGAGTAC 18980 0.0 18.196068 10-11 ACGCAGA 23835 0.0 18.141264 7 CGCAGAG 23855 0.0 18.086304 8 CATGGGA 8585 0.0 18.075583 4 >>END_MODULE