FastQCFastQC Report
Fri 27 May 2016
SRR939477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281150
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87020.20326314191280379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69050.16128843885404623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.10079067540263713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88000.041.6909371
GTATCAA149700.039.373021
TATCAAC188250.030.9712312
ATCAACG186200.030.8370043
TCAACGC187250.030.7949314
CAACGCA191400.030.1510335
AACGCAG194500.029.5928466
ACGCAGA217250.025.770757
CGCAGAG219600.025.2647538
GCAGAGT224450.022.6925899
GAGTACT119200.021.36895412-13
AGAGTAC190000.020.69457410-11
GTACATG142150.020.5910841
TACATGG141450.019.9877872
GTACTTT130250.019.96610314-15
AGTACTT127500.019.05697612-13
CAGAGTA220550.018.90816510-11
ACATGGG148150.018.7314723
ACTTTTT166850.017.12643616-17
GTGTAGC28400.016.4126171
Produced by FastQC (version 0.11.5)