##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939477_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4281150 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.927861439099306 31.0 26.0 31.0 10.0 33.0 2 27.084866215853218 31.0 26.0 31.0 10.0 34.0 3 27.584678883010405 31.0 26.0 31.0 16.0 34.0 4 31.267381661469464 35.0 30.0 37.0 19.0 37.0 5 31.552390595984722 35.0 32.0 37.0 19.0 37.0 6 31.549084241383742 35.0 32.0 37.0 17.0 37.0 7 30.989626151851724 35.0 30.0 37.0 17.0 37.0 8 30.908998516753677 35.0 30.0 37.0 17.0 37.0 9 31.913648669165994 35.0 30.0 39.0 17.0 39.0 10-11 31.607030937948917 35.0 30.0 39.0 16.0 39.0 12-13 31.430340679490325 35.0 29.5 39.0 16.0 39.0 14-15 32.16588241477173 36.0 29.5 39.0 16.5 40.5 16-17 32.10761734580662 36.0 30.0 39.0 16.5 40.0 18-19 32.227237307732736 36.0 30.0 39.0 16.5 40.5 20-21 31.984414701657265 36.0 29.0 39.0 11.0 41.0 22-23 31.81257314039452 36.0 29.0 39.0 11.0 41.0 24-25 31.696329023743623 36.0 29.0 39.0 10.5 41.0 26-27 31.731756887752123 36.0 29.0 39.0 11.0 41.0 28-29 31.755183537133714 36.0 29.5 39.0 10.5 41.0 30-31 31.44881842495591 36.0 28.5 39.0 10.0 40.5 32-33 30.893483409831468 35.5 27.0 39.0 9.0 40.0 34-35 30.4962578979947 35.0 26.5 39.0 9.0 40.0 36-37 30.272599301589526 35.0 25.5 39.0 9.0 40.0 38-39 29.865628394239863 35.0 25.0 38.0 8.0 40.0 40-41 29.77294278406503 35.0 25.0 38.0 8.0 40.0 42-43 29.324486177779335 34.0 24.5 38.0 4.5 40.0 44-45 29.148463496957593 34.0 24.5 38.0 2.0 40.0 46-47 28.560171566051178 34.0 24.0 38.0 2.0 40.0 48-49 28.01563049647875 33.0 22.5 38.0 2.0 40.0 50-51 27.551476122070007 33.0 22.5 37.5 2.0 39.5 52-53 27.93531235766091 33.5 23.0 38.0 2.0 39.5 54-55 27.812386274715905 33.5 22.5 38.0 2.0 40.0 56-57 27.29782721932191 33.0 21.0 38.0 2.0 40.0 58-59 26.783700991556007 33.0 19.0 38.0 2.0 40.0 60-61 26.202099202317136 32.0 16.5 37.0 2.0 39.0 62-63 25.634397182999894 31.0 13.5 37.0 2.0 39.0 64-65 25.08090256122771 31.0 11.5 36.0 2.0 39.0 66-67 24.60487801174918 31.0 8.0 36.0 2.0 39.0 68-69 24.1153201826612 30.0 7.5 35.5 2.0 38.0 70-71 23.583104773250177 30.0 4.5 35.0 2.0 38.0 72-73 22.900123448138935 29.0 2.0 34.5 2.0 37.5 74-75 22.227743129766537 29.0 2.0 34.0 2.0 36.5 76-77 21.525155390490873 28.0 2.0 34.0 2.0 36.0 78-79 20.74489821660068 26.5 2.0 33.5 2.0 35.5 80-81 19.976274131950525 26.0 2.0 33.0 2.0 35.0 82-83 19.099217032806607 25.5 2.0 32.0 2.0 35.0 84-85 18.266709996145895 24.0 2.0 32.0 2.0 34.0 86-87 17.62417142590192 21.5 2.0 31.5 2.0 34.0 88-89 16.733349333707068 19.0 2.0 31.0 2.0 34.0 90-91 15.828975859290143 11.5 2.0 31.0 2.0 34.0 92-93 14.90846384733074 4.5 2.0 30.0 2.0 34.0 94-95 13.967542365953074 2.0 2.0 29.5 2.0 34.0 96-97 12.902581549350057 2.0 2.0 29.0 2.0 33.5 98-99 11.859233500344533 2.0 2.0 28.5 2.0 33.0 100-101 10.58824813426299 2.0 2.0 25.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.8805634563114673 1101 2 3.7290751509792877 1101 3 3.2078802597927094 1101 4 3.2793807616092643 1101 5 2.816593971885194 1101 6 2.7303099593354005 1101 7 3.1752189355851215 1101 8 3.0508631876135084 1101 9 3.281568711664331 1101 10-11 3.4047188231963297 1101 12-13 3.242766609332893 1101 14-15 3.09668790058252 1101 16-17 2.872718435525531 1101 18-19 3.096230527643975 1101 20-21 2.953930723720884 1101 22-23 3.130151208359724 1101 24-25 3.2747656942696004 1101 26-27 3.3298349387623425 1101 28-29 2.9870678660699 1101 30-31 2.9738457284112627 1101 32-33 3.36544698070108 1101 34-35 3.345784132506065 1101 36-37 3.3130772680677723 1101 38-39 3.3128331891406653 1101 40-41 3.27347850445841 1101 42-43 3.2115154518815707 1101 44-45 3.094570842809464 1101 46-47 3.408609560082315 1101 48-49 3.108800415374411 1101 50-51 3.0281939919723833 1101 52-53 2.7945549023485015 1101 54-55 2.924232678811954 1101 56-57 3.022573223179247 1101 58-59 2.9780491813289522 1101 60-61 2.7367661821562663 1101 62-63 2.6591988175787584 1101 64-65 2.60308240421627 1101 66-67 2.6990660766401575 1101 68-69 2.5758645487797054 1101 70-71 2.3443249040191994 1101 72-73 2.0741986590654022 1101 74-75 2.1194686705503294 1101 76-77 1.8479866900666373 1101 78-79 1.8499367943654725 1101 80-81 1.94436015109331 1101 82-83 1.8658423319860837 1101 84-85 1.8624148737130248 1101 86-87 1.524568560320425 1101 88-89 1.3686689292768754 1101 90-91 1.3082504968295385 1101 92-93 1.209735429053202 1101 94-95 0.9814061199319593 1101 96-97 0.999439973633125 1101 98-99 0.740417324470366 1101 100-101 0.4538720266353149 1102 1 3.946016595398426 1102 2 3.7391665369312577 1102 3 3.1906301368519685 1102 4 3.179015012900141 1102 5 2.776136222520389 1102 6 2.4447006855053885 1102 7 3.130422402514057 1102 8 3.09835673322452 1102 9 3.301408273995289 1102 10-11 3.401266237342714 1102 12-13 3.5462791287985063 1102 14-15 3.434124381198867 1102 16-17 3.115190876309075 1102 18-19 3.1038310829263267 1102 20-21 3.2355354589884833 1102 22-23 3.3229722373737367 1102 24-25 3.4427667802461563 1102 26-27 3.2308522734070486 1102 28-29 3.1155166100253098 1102 30-31 3.2672169172482484 1102 32-33 3.457128758834017 1102 34-35 3.6710584440379 1102 36-37 3.5349571344721653 1102 38-39 3.3608543554385086 1102 40-41 3.4191261460451727 1102 42-43 3.3309997666918356 1102 44-45 2.980028489723651 1102 46-47 3.0608166025378516 1102 48-49 3.186847296777575 1102 50-51 3.1252633920215587 1102 52-53 2.8288886659744357 1102 54-55 3.0216849880489 1102 56-57 3.1276542616187193 1102 58-59 2.985827744561476 1102 60-61 3.1844446511342213 1102 62-63 2.9573211538626687 1102 64-65 2.9278547077159836 1102 66-67 2.6260994652968286 1102 68-69 2.505797289948461 1102 70-71 2.320815048269669 1102 72-73 2.3651430488695198 1102 74-75 2.373792086663766 1102 76-77 2.272385817187363 1102 78-79 2.2085357309280518 1102 80-81 1.9668723939422534 1102 82-83 1.9058838757120071 1102 84-85 1.9051303785896714 1102 86-87 1.4167239164387766 1102 88-89 1.2037259942715473 1102 90-91 1.1225011217783116 1102 92-93 1.1556581505514387 1102 94-95 1.0672097938111502 1102 96-97 0.7627060481352164 1102 98-99 0.4447051389937666 1102 100-101 0.06748335972276642 1103 1 3.854502808267327 1103 2 3.746554979932746 1103 3 3.002999401778162 1103 4 3.0558755254571857 1103 5 2.617382198380586 1103 6 2.732263116337677 1103 7 3.1300033487067935 1103 8 3.0033336515357405 1103 9 3.288907626391204 1103 10-11 3.2424026601792484 1103 12-13 3.381750140240957 1103 14-15 3.244763149081731 1103 16-17 2.9993244773907115 1103 18-19 3.03852248249229 1103 20-21 3.183951747706214 1103 22-23 3.21332038662921 1103 24-25 3.3213102089355964 1103 26-27 3.176647542011633 1103 28-29 3.057648755616782 1103 30-31 3.161523006777351 1103 32-33 3.3688749162032465 1103 34-35 3.4123872277726797 1103 36-37 3.505688892904331 1103 38-39 3.3568638100983073 1103 40-41 3.3274087908674232 1103 42-43 3.2195176698865318 1103 44-45 3.0287360068959543 1103 46-47 3.0068902284659025 1103 48-49 3.2027476859156714 1103 50-51 3.1552869917935986 1103 52-53 2.791557213930954 1103 54-55 2.8608537513337815 1103 56-57 2.8615672815548514 1103 58-59 2.8628547003617797 1103 60-61 2.941163723362223 1103 62-63 2.8315163933610528 1103 64-65 2.678037768300065 1103 66-67 2.6693222031438175 1103 68-69 2.7588252212923514 1103 70-71 2.5770301136273126 1103 72-73 2.521468107981377 1103 74-75 2.433417983775307 1103 76-77 2.288877190021669 1103 78-79 2.405452231556229 1103 80-81 2.0998023283975265 1103 82-83 2.0257768204613065 1103 84-85 2.015076124658357 1103 86-87 1.7685439177627664 1103 88-89 1.879749145757657 1103 90-91 1.6141586545290902 1103 92-93 1.454794278188448 1103 94-95 1.3248775835312276 1103 96-97 1.3538530170243153 1103 98-99 0.967972631679439 1103 100-101 0.813341144607401 1104 1 3.84445426241998 1104 2 3.7506426547196696 1104 3 3.226709865726548 1104 4 3.1777074345576786 1104 5 2.73794413864724 1104 6 2.4570457162266663 1104 7 3.1210063953821034 1104 8 3.010671352661234 1104 9 3.3151791343193047 1104 10-11 3.4245546539035523 1104 12-13 3.5854189597780426 1104 14-15 3.3410316444184183 1104 16-17 3.1117126858499837 1104 18-19 3.0276439338052086 1104 20-21 3.2742933450781813 1104 22-23 3.2686095942963966 1104 24-25 3.1810077947537856 1104 26-27 3.046654222722278 1104 28-29 2.8432299887457475 1104 30-31 3.1073917874791803 1104 32-33 3.3203117166494494 1104 34-35 3.4902043652913797 1104 36-37 3.3623082902078636 1104 38-39 3.4313362486986456 1104 40-41 3.2316876767856826 1104 42-43 3.2195546347421278 1104 44-45 3.025224655870815 1104 46-47 3.1645815489626834 1104 48-49 3.3076273970011947 1104 50-51 2.8964271607974545 1104 52-53 2.8993061557233197 1104 54-55 2.8556997025141975 1104 56-57 3.172305536647901 1104 58-59 3.2221820835943866 1104 60-61 3.1148936692078237 1104 62-63 3.003405941486079 1104 64-65 2.9570493404716593 1104 66-67 2.7014067448711323 1104 68-69 2.740594921288867 1104 70-71 2.761080257118767 1104 72-73 2.7019009921473938 1104 74-75 2.533137330103692 1104 76-77 2.476212374523058 1104 78-79 2.44936390930814 1104 80-81 2.5518460793623667 1104 82-83 2.52325279452403 1104 84-85 2.39636034402022 1104 86-87 2.1895417330688858 1104 88-89 2.210992768045795 1104 90-91 2.049946223797752 1104 92-93 2.015190818898475 1104 94-95 1.7751475532958363 1104 96-97 1.5283786158738142 1104 98-99 1.0477758102226602 1104 100-101 0.8558783413797926 1105 1 3.802838103071263 1105 2 3.518196753669116 1105 3 2.0148915274547186 1105 4 2.814812355092837 1105 5 2.6465483408560893 1105 6 2.7164980672487857 1105 7 3.2324928818685947 1105 8 3.0673143744205333 1105 9 3.3091799873317846 1105 10-11 3.5810637377382157 1105 12-13 3.7552601049098833 1105 14-15 3.492535827172599 1105 16-17 3.303941667323148 1105 18-19 3.1684396298334576 1105 20-21 3.446872173907014 1105 22-23 3.66557279503866 1105 24-25 3.386233992712544 1105 26-27 3.4923466967577745 1105 28-29 3.553340431414334 1105 30-31 3.374500673774108 1105 32-33 3.805607539881322 1105 34-35 4.037285603506337 1105 36-37 3.9726845510201727 1105 38-39 3.9797091648041096 1105 40-41 3.696418603725917 1105 42-43 3.685801716541537 1105 44-45 3.608706705195889 1105 46-47 3.6519537841546814 1105 48-49 3.6826994166953106 1105 50-51 3.503348346933752 1105 52-53 3.2588443691859545 1105 54-55 3.473287285944803 1105 56-57 3.5354269637228164 1105 58-59 3.662075569272755 1105 60-61 3.8317907988956463 1105 62-63 3.747571410942246 1105 64-65 3.6749650344047957 1105 66-67 3.749376537753715 1105 68-69 3.8329681292144 1105 70-71 3.689783565909284 1105 72-73 3.5440506584249647 1105 74-75 3.6193673516534837 1105 76-77 3.640578152260929 1105 78-79 3.5465830137946845 1105 80-81 3.5621327364513498 1105 82-83 3.583048782372348 1105 84-85 3.3936235956439376 1105 86-87 3.0527239559894817 1105 88-89 3.2229723556067817 1105 90-91 3.4523960157824245 1105 92-93 3.043386061483254 1105 94-95 2.632436320468324 1105 96-97 2.4987570092173232 1105 98-99 2.235934275997405 1105 100-101 2.171019687044394 1106 1 3.2575249292615673 1106 2 2.4455432932412577 1106 3 2.5727847821992036 1106 4 1.233663803880713 1106 5 1.5707205359965535 1106 6 1.969267435881548 1106 7 2.5905255559249554 1106 8 2.5324772272165106 1106 9 2.977709214932407 1106 10-11 2.7561197987049546 1106 12-13 2.920315277525905 1106 14-15 2.7218911389923157 1106 16-17 1.4689878701845025 1106 18-19 2.5625495196779724 1106 20-21 1.8474337969841237 1106 22-23 2.6366433093850965 1106 24-25 2.646614370684201 1106 26-27 2.7479902033453705 1106 28-29 2.1105629813775906 1106 30-31 1.4489607635406543 1106 32-33 1.6757839896179192 1106 34-35 2.2762639958848077 1106 36-37 2.483045458586851 1106 38-39 1.0731724992069225 1106 40-41 1.232902649637257 1106 42-43 2.3636258739883296 1106 44-45 2.5747432681583895 1106 46-47 2.5687582098661395 1106 48-49 2.7443612836807496 1106 50-51 2.547646278197835 1106 52-53 2.170875203580131 1106 54-55 2.198833686313254 1106 56-57 2.556612316803413 1106 58-59 2.8323979068943217 1106 60-61 2.859967040775434 1106 62-63 2.4462291820272064 1106 64-65 2.600388919999027 1106 66-67 2.6317235646250303 1106 68-69 2.8127411383600993 1106 70-71 2.573889915288131 1106 72-73 2.4303720844089653 1106 74-75 2.3784652487634936 1106 76-77 2.4000482295043177 1106 78-79 2.4365053650433772 1106 80-81 2.4268790562645037 1106 82-83 2.306280899786433 1106 84-85 2.275415295515945 1106 86-87 1.97014302551543 1106 88-89 2.053013271487732 1106 90-91 2.1574799692363005 1106 92-93 2.073422643420237 1106 94-95 1.989400558746409 1106 96-97 2.0247363080584986 1106 98-99 1.8636259355055174 1106 100-101 1.5911714915175423 1107 1 2.63108014915219 1107 2 2.913185661644892 1107 3 -0.39502575299914255 1107 4 1.6604545462150817 1107 5 0.5043701432618946 1107 6 1.3426556256913784 1107 7 2.2830595520293713 1107 8 1.4560639742020456 1107 9 -1.7137836849518457 1107 10-11 -0.8309335646643738 1107 12-13 1.7282764206321737 1107 14-15 1.5997036199774435 1107 16-17 -0.5066395123177294 1107 18-19 0.728719041168219 1107 20-21 0.7048807107723896 1107 22-23 0.7266611557936535 1107 24-25 0.016916248468731965 1107 26-27 0.6066388243442269 1107 28-29 0.9647318796811462 1107 30-31 1.52560115874266 1107 32-33 -0.15538450548651994 1107 34-35 1.2024306154112843 1107 36-37 2.1116370900989736 1107 38-39 -0.012869723697331636 1107 40-41 0.8141041428781577 1107 42-43 0.316851500334419 1107 44-45 0.7724049138928031 1107 46-47 0.9063838418289301 1107 48-49 0.5692838032539598 1107 50-51 1.4427650948920174 1107 52-53 1.5713082915838577 1107 54-55 1.0278975221610658 1107 56-57 1.001229408032728 1107 58-59 1.122479676744522 1107 60-61 1.698494153742729 1107 62-63 2.0125070925330526 1107 64-65 1.9351739707399283 1107 66-67 1.2555252174070723 1107 68-69 0.8130722564385984 1107 70-71 0.4478036012568687 1107 72-73 0.9703467691212992 1107 74-75 0.8966480577708147 1107 76-77 0.8510744481452228 1107 78-79 1.6344239749893568 1107 80-81 1.7992660685724395 1107 82-83 1.727164541068941 1107 84-85 1.8310231399118457 1107 86-87 1.5712971913379192 1107 88-89 1.7808346790203977 1107 90-91 1.8261328763152864 1107 92-93 1.864764962142786 1107 94-95 1.6804940039858831 1107 96-97 1.542689367122824 1107 98-99 1.3753737401431767 1107 100-101 1.2997720696368678 1108 1 2.9816006574388965 1108 2 3.1928020000966413 1108 3 2.6857461354643704 1108 4 2.7254370935990018 1108 5 2.238506353382224 1108 6 2.018774502010821 1108 7 1.9754687757779266 1108 8 2.44865282504162 1108 9 2.6447763300053744 1108 10-11 -0.3513938709597504 1108 12-13 2.004447875771323 1108 14-15 1.5190634883362613 1108 16-17 1.685799243132383 1108 18-19 0.19652122585328868 1108 20-21 0.006929026838971453 1108 22-23 0.8638685389668588 1108 24-25 -0.2592265010304331 1108 26-27 0.7962518296374874 1108 28-29 0.33401059437338176 1108 30-31 0.04940364136059827 1108 32-33 1.4843642561439125 1108 34-35 -0.7655466943291813 1108 36-37 0.8766614637343366 1108 38-39 1.478770051222913 1108 40-41 2.23869456433615 1108 42-43 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-2.0822400152693668 2312 66-67 1.1701669119870388 2312 68-69 -0.4247620130996843 2312 70-71 -1.3258584047653876 2312 72-73 -1.3200277141472299 2312 74-75 -1.1754387234487425 2312 76-77 -1.5425793515724457 2312 78-79 0.5423667994131378 2312 80-81 0.815664134196048 2312 82-83 -0.7501494510802473 2312 84-85 -1.2720531150000696 2312 86-87 -0.23145398452930266 2312 88-89 -2.1013212650167823 2312 90-91 -2.0573386626931676 2312 92-93 -1.6210672631717422 2312 94-95 -1.129337334655622 2312 96-97 -0.8134719591253816 2312 98-99 -0.30839063056405713 2312 100-101 -0.2233503415462028 2313 1 -1.908356288116213 2313 2 -0.85069231409814 2313 3 -2.3674149424132196 2313 4 -2.18105159083386 2313 5 -1.6845683236152489 2313 6 -3.0910662313935298 2313 7 0.4760924106054034 2313 8 -2.890353409422847 2313 9 -3.959197752556303 2313 10-11 -4.966733253202403 2313 12-13 -3.7658659384795072 2313 14-15 -3.904098220617197 2313 16-17 -2.166359177633211 2313 18-19 -4.320753392934503 2313 20-21 -6.452056598759725 2313 22-23 -5.430730581859681 2313 24-25 -1.8589423285074744 2313 26-27 -5.1359833374892965 2313 28-29 -5.7888029867561315 2313 30-31 -4.946573108674016 2313 32-33 -4.523072831714096 2313 34-35 0.30140706476372614 2313 36-37 0.6540776130600712 2313 38-39 -2.4438964986476215 2313 40-41 -6.213469507855159 2313 42-43 -2.80388937068118 2313 44-45 -1.102424722838908 2313 46-47 -2.954013872603749 2313 48-49 -2.9489992224468544 2313 50-51 -2.4418514825626367 2313 52-53 -4.188125441430643 2313 54-55 -3.420295686379461 2313 56-57 -2.6525180165436453 2313 58-59 -0.725442633633854 2313 60-61 0.937341801183667 2313 62-63 0.814955766077599 2313 64-65 1.4736401860042143 2313 66-67 0.835666821975181 2313 68-69 -2.202411582807688 2313 70-71 -0.537501615400199 2313 72-73 -1.9711484941794204 2313 74-75 -2.2042612712432756 2313 76-77 -1.3642314398476074 2313 78-79 -0.9358524924229634 2313 80-81 -1.1630564884036367 2313 82-83 -1.0185099291667719 2313 84-85 -1.6307681162708256 2313 86-87 -1.3842661491909993 2313 88-89 -2.1470919430355337 2313 90-91 -3.6158266487572135 2313 92-93 -3.964253762451648 2313 94-95 -3.236714600836576 2313 96-97 -2.673652079917767 2313 98-99 -2.0314180029943056 2313 100-101 -1.751989335743085 2314 1 -2.3409129152117885 2314 2 -0.5752816303953736 2314 3 -2.503390195161437 2314 4 -1.3606400282207822 2314 5 -2.7308441815815527 2314 6 -2.6975885062907317 2314 7 -1.0792372075986414 2314 8 0.5957200442205632 2314 9 -1.4781188353559145 2314 10-11 -2.121515263317253 2314 12-13 -1.1023622307303818 2314 14-15 -3.5147282941996565 2314 16-17 -1.8412863041153393 2314 18-19 -1.04104244484299 2314 20-21 -1.2905442826096483 2314 22-23 -1.9570165305214822 2314 24-25 -2.1450118013357304 2314 26-27 -1.8079270442901851 2314 28-29 -1.5440570142004972 2314 30-31 -1.323234841813587 2314 32-33 -7.975661054852651 2314 34-35 -8.468529014079671 2314 36-37 -4.647799363649476 2314 38-39 -3.1213297495467707 2314 40-41 -2.160981923301975 2314 42-43 -1.4007512359480323 2314 44-45 -1.1470180378425958 2314 46-47 -2.330500092050432 2314 48-49 -1.8716421437747677 2314 50-51 -1.4756256576281537 2314 52-53 -0.9924986576117156 2314 54-55 0.06971492763667797 2314 56-57 -0.2839579578959537 2314 58-59 -2.8396145592257085 2314 60-61 -3.852653023756634 2314 62-63 -3.3642232288157707 2314 64-65 -3.9420797399436758 2314 66-67 -3.6454049387035816 2314 68-69 -4.647966734317961 2314 70-71 -6.61262679861834 2314 72-73 -6.538330654044483 2314 74-75 -6.289885697882891 2314 76-77 -7.390603445681961 2314 78-79 -6.504469713787149 2314 80-81 -5.809734050874479 2314 82-83 -4.731622152818808 2314 84-85 -4.6071852752838165 2314 86-87 -4.481228921412782 2314 88-89 -4.712797891402126 2314 90-91 -5.033997951731687 2314 92-93 -5.294909483218486 2314 94-95 -4.755153199893796 2314 96-97 -4.907284242757261 2314 98-99 -4.869890568692293 2314 100-101 -4.517549067427197 2315 1 -24.51423000324103 2315 2 -12.930516494879129 2315 3 -8.37735711230479 2315 4 -5.94523075385397 2315 5 -2.5931555676514435 2315 6 -2.6377948711479924 2315 7 -8.295500479159479 2315 8 -2.1505864458835156 2315 9 -5.670403163434443 2315 10-11 -3.6586110524713753 2315 12-13 -4.188067159800408 2315 14-15 -3.81656270498787 2315 16-17 -5.819902405577974 2315 18-19 -7.154152425585593 2315 20-21 -5.7302978672468505 2315 22-23 -7.378853616089518 2315 24-25 -23.542948712127753 2315 26-27 -10.68890046074171 2315 28-29 -7.143020613294734 2315 30-31 -5.556507679017635 2315 32-33 -6.081977908457272 2315 34-35 -7.144007588635599 2315 36-37 -5.9357865511490004 2315 38-39 -4.362105750974674 2315 40-41 -4.264412709814373 2315 42-43 -3.6470322495370304 2315 44-45 -8.566859819022216 2315 46-47 -5.304427802820289 2315 48-49 -4.82386457561746 2315 50-51 -6.278335931681212 2315 52-53 -10.137569816416853 2315 54-55 -6.373031155921208 2315 56-57 -12.751346950719302 2315 58-59 -8.323786422474289 2315 60-61 -5.621287975806375 2315 62-63 -5.879245778836953 2315 64-65 -6.0176718146625845 2315 66-67 -8.665414275007308 2315 68-69 -5.875740356412255 2315 70-71 -5.96692976167434 2315 72-73 -6.244366706618656 2315 74-75 -5.4997785185399195 2315 76-77 -6.301833757954878 2315 78-79 -5.670940585892499 2315 80-81 -4.99423522731994 2315 82-83 -4.358561508034146 2315 84-85 -4.365965545172152 2315 86-87 -4.171347898263733 2315 88-89 -5.328545123691498 2315 90-91 -5.236188779474341 2315 92-93 -5.10512658279098 2315 94-95 -5.34185679818458 2315 96-97 -5.472034356791363 2315 98-99 -5.062010596131669 2315 100-101 -4.42319097136192 2316 1 -1.358041398132972 2316 2 0.413370992780397 2316 3 -4.215589792180548 2316 4 0.15805304265035502 2316 5 -4.62272843139619 2316 6 -0.3365777148527904 2316 7 -3.379508534497287 2316 8 -3.094572329363185 2316 9 -1.8879310668491058 2316 10-11 -1.2907853377077387 2316 12-13 -3.6130974165150995 2316 14-15 -2.832170741958457 2316 16-17 -0.9573757977082771 2316 18-19 -5.242782132595639 2316 20-21 -1.813335083825887 2316 22-23 -4.805653193643462 2316 24-25 -1.6994116775809793 2316 26-27 -1.3496051060083794 2316 28-29 -3.9948486436400046 2316 30-31 -8.407707780037349 2316 32-33 -5.421109926129162 2316 34-35 -2.5464241899783744 2316 36-37 -4.115969468978648 2316 38-39 -4.235342251888117 2316 40-41 -2.1569736578253043 2316 42-43 -2.2153159968434473 2316 44-45 0.05130044152991431 2316 46-47 -1.6444601422465084 2316 48-49 -1.7722353048288824 2316 50-51 0.36990950685255797 2316 52-53 1.3937362896997634 2316 54-55 1.6967408281649092 2316 56-57 -0.09385558495252155 2316 58-59 -2.5278975638329975 2316 60-61 -5.702727408259282 2316 62-63 -1.4634666971323433 2316 64-65 -2.906564730491521 2316 66-67 -1.643502837805162 2316 68-69 1.0289742793100203 2316 70-71 1.941679872588395 2316 72-73 2.430942502246154 2316 74-75 2.2943287197845983 2316 76-77 1.7322348108123222 2316 78-79 1.6117191187232471 2316 80-81 2.0807049972193212 2316 82-83 1.8928002901166678 2316 84-85 1.4226790852039493 2316 86-87 0.9824582160525317 2316 88-89 -1.0261987113670923 2316 90-91 -0.9551306308244953 2316 92-93 -1.3312824876881386 2316 94-95 -2.9917265877159203 2316 96-97 -4.100898934624096 2316 98-99 -2.194199968727121 2316 100-101 -1.383494489668463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71434.0 3 23537.0 4 23532.0 5 26035.0 6 28296.0 7 34398.0 8 42303.0 9 49842.0 10 56466.0 11 62922.0 12 68775.0 13 74697.0 14 80209.0 15 85803.0 16 90674.0 17 94179.0 18 93447.0 19 96511.0 20 102766.0 21 109977.0 22 117882.0 23 126804.0 24 136547.0 25 145854.0 26 157316.0 27 168146.0 28 178700.0 29 191481.0 30 203639.0 31 215985.0 32 229247.0 33 241030.0 34 248191.0 35 245864.0 36 216953.0 37 123478.0 38 18164.0 39 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.959785512411344 19.432313301283408 15.063722873055735 22.544178313249514 2 15.760877462705219 20.017941407547983 40.69919176477769 23.521989364969112 3 19.602234828925276 24.392364861817768 29.41777856795944 26.58762174129751 4 12.964309013033388 17.038489531157794 37.379992082972706 32.61720937283611 5 14.037816169394329 36.94829016968721 34.223748852452985 14.790144808465472 6 31.72026564927056 35.87757196304783 18.02012341400192 14.382038973679693 7 28.5513499581036 32.31898235025009 20.863289338151024 18.266378353495284 8 26.15992984623218 34.83007277039021 19.788996513527465 19.221000869850148 9 26.375410245980348 17.35555400136462 20.575091709610287 35.693944043044745 10-11 24.95774364078219 26.710997648839303 27.31979360185573 21.011465108522778 12-13 26.16734842724846 24.034942471998438 27.57890428382875 22.21880481692435 14-15 23.166536063375055 25.47684501783214 26.133771542931655 25.22284737586115 16-17 22.27309231217774 28.49372283646933 26.856125136055205 22.377059715297722 18-19 22.12979718072043 27.100736862210777 29.201880174711636 21.567585782357156 20-21 23.256420898757373 26.137917367777774 29.267087770064254 21.338573963400595 22-23 22.722037726697636 26.268186099324915 28.94735172418979 22.06242444978766 24-25 22.382630700624546 26.432990169228145 29.23764806959238 21.94673106055493 26-27 22.32016527377859 26.64463796614931 29.093229861647586 21.941966898424514 28-29 22.17055924200575 26.79704935707099 29.150820978993963 21.881570421929297 30-31 22.420112834203007 26.526254930864035 29.093926486310618 21.959705748622344 32-33 22.28255182148047 26.601584054849848 28.990577657246043 22.125286466423642 34-35 22.656443130502034 26.680625671381165 28.883437998427375 21.779493199689426 36-37 22.536044932066567 26.511483936612873 28.957558394669654 21.994912736650914 38-39 22.495960655490435 26.682896261248068 28.907396102156973 21.913746981104524 40-41 22.64733412345859 26.616050877135056 28.823799959205743 21.91281504020061 42-43 22.480455410812027 26.64506436221281 28.578130844008875 22.296349382966284 44-45 22.555933395502024 26.779017212912322 28.485988822036685 22.17906056954897 46-47 22.59779938080814 26.878389679854823 28.246023317884354 22.277787621452678 48-49 22.781498228766083 26.905975662762984 28.044836712408415 22.267689396062522 50-51 22.531302222807557 27.19963709264381 27.965266254005883 22.30379443054275 52-53 22.52927920385576 27.373769402280608 27.702104430702118 22.394846963161516 54-55 22.4831002238858 27.191097040408955 27.880791793235637 22.445010942469608 56-57 22.440682155504284 27.43234819994284 27.749848383100268 22.377121261452608 58-59 22.605664779670573 27.23634313052406 27.72395786830798 22.434034221497388 60-61 22.637269007906824 27.243228266644763 27.85303323506111 22.266469490387305 62-63 22.488652505667606 27.208295282170575 27.886372267072485 22.416679945089335 64-65 22.443315651968124 27.131406238831673 27.840516994805085 22.584761114395114 66-67 22.40916448702776 27.256814016301806 27.686618723385838 22.647402773284597 68-69 22.411070412594345 27.16927488722621 27.784066390274543 22.635588309904904 70-71 22.39550147950857 27.032582187411908 27.781259043245278 22.79065728983425 72-73 22.33164799943048 27.112222255657514 27.782683138531123 22.773446606380883 74-75 22.279585746522702 27.29154761182922 27.56386470204432 22.86500193960376 76-77 22.417001327014262 27.10935512865788 27.58385900242285 22.88978454190501 78-79 22.25955555118589 27.16209777181006 27.581784628320744 22.996562048683302 80-81 22.23584998972845 27.28766132331501 27.4292038583568 23.04728482859974 82-83 22.382266467522232 27.242008018380982 27.399233470313423 22.976492043783367 84-85 22.342177711576962 27.160951423820094 27.297754908405803 23.199115956197137 86-87 22.18150898857234 27.185870396327573 27.45360489601079 23.179015719089296 88-89 22.289712976667595 27.020182161844875 27.372735732387415 23.317369129100115 90-91 22.177079368557166 27.15943655351576 27.385104005295887 23.278380072631187 92-93 22.280777781457655 27.049887003712474 27.337869256665705 23.331465958164166 94-95 22.11741842938349 27.04780941216347 27.306891566580934 23.527880591872105 96-97 22.135123298742858 26.866643120651627 27.298911900169266 23.69932168043625 98-99 21.974686134955764 26.88355322346554 27.10231155101106 24.039449090567633 100-101 22.09020933277229 26.8869423095486 27.145621086783557 23.877227270895556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4905.0 1 2834.5 2 722.0 3 677.0 4 628.5 5 626.5 6 841.0 7 1370.5 8 2077.0 9 2548.0 10 2523.0 11 2475.0 12 2537.5 13 2729.0 14 3075.5 15 3345.5 16 3777.0 17 4257.5 18 4747.0 19 5413.0 20 6291.0 21 7339.5 22 8830.5 23 10939.5 24 13500.5 25 17071.0 26 22155.0 27 28366.5 28 34494.5 29 40627.5 30 48114.5 31 56905.5 32 66603.5 33 76708.0 34 86666.5 35 96436.5 36 107158.5 37 117174.0 38 123456.5 39 127136.0 40 131433.0 41 137333.0 42 142572.5 43 147840.5 44 153472.0 45 157724.0 46 160337.0 47 163190.0 48 167428.0 49 167457.0 50 162797.0 51 157684.5 52 152682.5 53 147486.5 54 140310.0 55 130303.0 56 119657.5 57 108659.0 58 96933.0 59 84879.0 60 71410.0 61 57529.0 62 45258.0 63 35044.5 64 26029.0 65 18855.0 66 13822.0 67 10213.5 68 7598.0 69 5528.5 70 4044.0 71 2949.0 72 2061.0 73 1487.0 74 1092.0 75 765.0 76 521.0 77 346.0 78 237.5 79 170.0 80 112.0 81 62.5 82 50.0 83 40.5 84 32.0 85 20.5 86 13.5 87 12.5 88 10.0 89 8.5 90 7.5 91 4.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.4427899045817125 2 3.5540450579867557 3 1.07067026383098 4 0.9263632435210165 5 0.9479462294009786 6 0.11996776567043901 7 1.7644324538967333 8 0.9119045116382279 9 0.13947187087581608 10-11 1.005115447952069 12-13 0.8155168587879424 14-15 0.6347243147285192 16-17 0.1348119080153697 18-19 0.1479625801478575 20-21 0.16308702101070974 22-23 0.6003527089683847 24-25 1.3638625135769595 26-27 0.22073508286324936 28-29 0.2307207175642059 30-31 0.20911437347441694 32-33 0.22673814278873666 34-35 0.692640995994067 36-37 0.277040047650748 38-39 0.2705698235287247 40-41 0.25556217371500645 42-43 0.20116090302839193 44-45 0.17038646158158438 46-47 0.2057157539446177 48-49 0.608843418240426 50-51 0.23562594162783362 52-53 0.25621620359015684 54-55 0.2857293017063172 56-57 0.20716396295387923 58-59 0.19409504455578525 60-61 0.1825794471111734 62-63 0.17049157352580496 64-65 0.16501407332142065 66-67 0.17979982014178433 68-69 0.1867255293554302 70-71 0.1933709400511545 72-73 0.25410228560083153 74-75 0.22561694871705032 76-77 0.22261541875430668 78-79 0.21663571703864618 80-81 0.22523153825490813 82-83 0.2385573969610969 84-85 0.20937131378251173 86-87 0.1973067984069701 88-89 0.19775060439367925 90-91 0.2138677691741705 92-93 0.20238720904429885 94-95 0.2136341870759025 96-97 0.23546243415904605 98-99 0.2495591137895192 100-101 0.30183478738189506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4281150.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.76786870954114 #Duplication Level Percentage of deduplicated Percentage of total 1 78.0786016870499 48.22748818030255 2 11.948395201125688 14.760538121456857 3 4.357789804698206 8.075141655611272 4 2.0540695907258812 5.075020032008686 5 1.0836840790899918 3.34684279599253 6 0.6656644535458154 2.4670004742735823 7 0.40701911879189207 1.7598492444267504 8 0.25504276775186774 1.2602758555052287 9 0.19994154505756848 1.1114966794228824 >10 0.8866921616848343 9.488088710158236 >50 0.04427872662778513 1.8562232636820066 >100 0.018348531110523425 1.8922092375391986 >500 2.755274560442949E-4 0.1200793871741344 >1k 1.1808317037296125E-4 0.18030139529603254 >5k 7.872211358195826E-5 0.37944496715007203 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8702 0.20326314191280379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6905 0.16128843885404623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4315 0.10079067540263713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.3358209826798872E-5 7 0.0 0.0 0.0 0.0 2.3358209826798872E-5 8 2.3358209826798872E-5 0.0 0.0 0.0 2.3358209826798872E-5 9 2.3358209826798872E-5 0.0 0.0 0.0 2.3358209826798872E-5 10-11 2.3358209826798872E-5 0.0 0.0 0.0 2.3358209826798872E-5 12-13 2.3358209826798872E-5 0.0 2.3358209826798872E-5 0.0 9.34328393071955E-5 14-15 2.3358209826798872E-5 0.0 2.3358209826798872E-5 1.1679104913399436E-5 1.1679104913399437E-4 16-17 2.3358209826798872E-5 0.0 2.3358209826798872E-5 2.3358209826798872E-5 1.6350746878759212E-4 18-19 2.3358209826798872E-5 0.0 2.3358209826798872E-5 5.839552456699718E-5 1.8686567861439098E-4 20-21 2.3358209826798872E-5 0.0 2.3358209826798872E-5 1.2847015404739381E-4 1.9854478352779043E-4 22-23 2.3358209826798872E-5 0.0 2.3358209826798872E-5 2.1022388844118986E-4 2.1022388844118986E-4 24-25 2.3358209826798872E-5 0.0 2.3358209826798872E-5 3.386940424885837E-4 2.1022388844118986E-4 26-27 2.3358209826798872E-5 0.0 2.3358209826798872E-5 5.022015112761758E-4 2.219029933545893E-4 28-29 2.3358209826798872E-5 0.0 2.3358209826798872E-5 0.0011679104913399436 2.3358209826798874E-4 30-31 2.3358209826798872E-5 0.0 2.3358209826798872E-5 0.003223432956098245 2.3358209826798874E-4 32-33 2.3358209826798872E-5 0.0 2.3358209826798872E-5 0.0077198883477570274 2.3358209826798874E-4 34-35 2.3358209826798872E-5 1.1679104913399436E-5 2.3358209826798872E-5 0.015089403548112073 2.3358209826798874E-4 36-37 2.3358209826798872E-5 2.3358209826798872E-5 2.3358209826798872E-5 0.026476530838676522 2.3358209826798874E-4 38-39 3.503731474019831E-5 2.3358209826798872E-5 2.3358209826798872E-5 0.043329479228711915 2.3358209826798874E-4 40-41 4.6716419653597745E-5 2.3358209826798872E-5 2.3358209826798872E-5 0.06284526353900237 2.3358209826798874E-4 42-43 4.6716419653597745E-5 2.3358209826798872E-5 2.3358209826798872E-5 0.08350560013080598 2.3358209826798874E-4 44-45 4.6716419653597745E-5 2.3358209826798872E-5 2.3358209826798872E-5 0.11090478025764106 2.3358209826798874E-4 46-47 4.6716419653597745E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.14468075166719224 2.3358209826798874E-4 48-49 4.6716419653597745E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.18361888744846594 2.5694030809478763E-4 50-51 4.6716419653597745E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.22497459794681335 2.802985179215865E-4 52-53 5.839552456699718E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.26664564427782256 3.0365672774838534E-4 54-55 7.007462948039662E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.3133270266166801 3.0365672774838534E-4 56-57 7.007462948039662E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.37120867056748774 3.153358326617848E-4 58-59 8.175373439379605E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.43600434462702775 3.2701493757518425E-4 60-61 9.343283930719549E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.5072118472840241 3.503731474019831E-4 62-63 9.343283930719549E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.5818413276806466 3.9708956705558087E-4 64-65 9.343283930719549E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.6649848755591372 4.204477768823797E-4 66-67 9.343283930719549E-5 4.6716419653597745E-5 2.3358209826798872E-5 0.7595272298331056 4.4380598670917863E-4 68-69 1.0511194422059493E-4 4.6716419653597745E-5 2.3358209826798872E-5 0.8692173831797532 4.4380598670917863E-4 70-71 1.4014925896079324E-4 4.6716419653597745E-5 2.3358209826798872E-5 0.9880289174637655 4.6716419653597754E-4 72-73 1.4014925896079324E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.1104025787463647 5.138806161895753E-4 74-75 1.4014925896079324E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.2382887775480886 5.372388260163741E-4 76-77 1.4014925896079324E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.3811709470586173 5.372388260163741E-4 78-79 1.6350746878759212E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.5438608785022716 5.372388260163741E-4 80-81 1.6350746878759212E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.721675250808778 5.372388260163741E-4 82-83 1.7518657370099155E-4 4.6716419653597745E-5 2.3358209826798872E-5 1.9070109666795136 5.839552456699718E-4 84-85 1.8686567861439098E-4 4.6716419653597745E-5 2.3358209826798872E-5 2.097450451397405 5.839552456699718E-4 86-87 1.8686567861439098E-4 4.6716419653597745E-5 2.3358209826798872E-5 2.304147250154748 6.189925604101702E-4 88-89 1.8686567861439098E-4 4.6716419653597745E-5 2.3358209826798872E-5 2.530231363068334 6.540298751503685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8800 0.0 41.690937 1 GTATCAA 14970 0.0 39.37302 1 TATCAAC 18825 0.0 30.971231 2 ATCAACG 18620 0.0 30.837004 3 TCAACGC 18725 0.0 30.794931 4 CAACGCA 19140 0.0 30.151033 5 AACGCAG 19450 0.0 29.592846 6 ACGCAGA 21725 0.0 25.77075 7 CGCAGAG 21960 0.0 25.264753 8 GCAGAGT 22445 0.0 22.692589 9 GAGTACT 11920 0.0 21.368954 12-13 AGAGTAC 19000 0.0 20.694574 10-11 GTACATG 14215 0.0 20.591084 1 TACATGG 14145 0.0 19.987787 2 GTACTTT 13025 0.0 19.966103 14-15 AGTACTT 12750 0.0 19.056976 12-13 CAGAGTA 22055 0.0 18.908165 10-11 ACATGGG 14815 0.0 18.731472 3 ACTTTTT 16685 0.0 17.126436 16-17 GTGTAGC 2840 0.0 16.412617 1 >>END_MODULE