FastQCFastQC Report
Fri 27 May 2016
SRR939477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281150
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124580.29099657802226037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113220.26446165165901686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71040.1659367226095792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98800.043.602881
GTATCAA161000.035.7650341
ATCAACG201550.028.3915673
TCAACGC203050.028.1118454
TATCAAC205400.027.8649312
CAACGCA207900.027.387685
AACGCAG213300.026.7165266
ACGCAGA244950.023.051767
CGCAGAG248400.022.6743858
GCAGAGT263150.020.9354159
GAGTACT140650.020.79781312-13
GTACATG161550.019.8604871
AGAGTAC226650.019.70907810-11
GTACTTT146750.019.691214-15
AGTACTT146200.019.3278512-13
TACATGG161200.018.9871142
CAGAGTA259450.018.24901810-11
ACATGGG158350.018.1882423
ACTTTTT178250.016.59666616-17
GTGGTAT60100.016.2442991
Produced by FastQC (version 0.11.5)