##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4281150 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99832404844493 31.0 31.0 31.0 30.0 34.0 2 31.26252712472116 31.0 31.0 34.0 30.0 34.0 3 31.263570068789928 31.0 31.0 34.0 28.0 34.0 4 35.18939350408185 35.0 35.0 37.0 33.0 37.0 5 34.803733809840814 35.0 35.0 37.0 32.0 37.0 6 34.759491258189975 35.0 35.0 37.0 32.0 37.0 7 34.63433306471392 35.0 35.0 37.0 30.0 37.0 8 34.64363453744905 35.0 35.0 37.0 30.0 37.0 9 36.00518925989512 37.0 35.0 39.0 30.0 39.0 10-11 35.86484040503136 37.0 35.0 39.0 30.0 39.0 12-13 35.83690421966061 37.0 35.0 39.0 30.0 39.0 14-15 36.73418345537998 38.0 36.0 40.0 31.0 41.0 16-17 36.72992688880325 38.0 36.0 40.0 31.0 41.0 18-19 36.65878081823809 38.0 35.0 40.0 30.0 41.0 20-21 36.55448080539108 38.0 35.0 40.0 30.0 41.0 22-23 36.41880476040316 38.0 35.0 40.0 30.0 41.0 24-25 36.20744029057613 38.0 34.0 40.0 29.5 41.0 26-27 35.83847856300293 38.0 34.0 40.0 28.0 41.0 28-29 35.558512782780326 38.0 34.0 40.0 27.0 41.0 30-31 35.36292468145241 38.0 34.0 40.0 27.0 41.0 32-33 35.188606449201735 38.0 33.5 40.0 27.0 41.0 34-35 35.18708419466732 38.0 34.0 40.0 27.0 41.0 36-37 35.23989442089158 38.0 34.0 40.0 26.5 41.0 38-39 35.2210658351144 38.0 34.0 40.0 27.0 41.0 40-41 35.01909521974236 38.0 33.0 40.0 26.0 41.0 42-43 34.744665568830804 38.0 33.0 40.0 25.0 41.0 44-45 34.593270616539954 38.0 33.0 40.0 25.0 41.0 46-47 34.326705791668125 37.5 33.0 40.0 24.0 41.0 48-49 34.054051715076554 37.0 33.0 40.0 24.0 41.0 50-51 33.809572427969115 37.0 32.5 40.0 23.0 41.0 52-53 33.45490837742196 37.0 32.0 40.0 22.5 41.0 54-55 33.234839821076115 37.0 31.0 39.5 22.0 41.0 56-57 32.83771077864593 36.0 31.0 39.0 20.5 40.0 58-59 32.409238055195445 36.0 31.0 39.0 18.5 40.0 60-61 32.1305533559908 35.5 30.0 39.0 18.5 40.0 62-63 31.73679245062658 35.0 30.0 38.5 17.0 40.0 64-65 31.29528970019738 35.0 29.0 38.0 13.5 40.0 66-67 29.573832498277334 33.5 26.5 37.5 9.5 39.5 68-69 25.844956962498394 30.5 17.0 35.5 6.5 39.0 70-71 25.219639349240275 29.5 16.0 35.0 5.5 38.0 72-73 27.087867161860714 31.0 22.5 35.0 6.5 38.0 74-75 27.717487006995785 32.0 25.0 35.0 6.0 38.0 76-77 27.791633906777385 31.5 26.0 34.5 3.5 36.5 78-79 28.638193709634095 33.0 27.0 35.0 2.0 37.0 80-81 28.494746271445756 33.0 27.0 35.0 2.0 37.0 82-83 28.0770869976525 33.0 26.0 35.0 2.0 36.0 84-85 27.732839774359693 33.0 26.0 35.0 2.0 36.0 86-87 27.275633649837076 32.0 26.0 35.0 2.0 35.5 88-89 26.78928909288392 32.0 25.0 34.0 2.0 35.0 90-91 26.33474814010254 32.0 25.0 34.0 2.0 35.0 92-93 25.763933288952735 31.5 23.5 34.0 2.0 35.0 94-95 24.36669282786167 31.0 18.0 34.0 2.0 35.0 96-97 23.549831820889246 30.5 7.0 34.0 2.0 35.0 98-99 23.211751281781765 31.0 2.0 34.0 2.0 35.0 100-101 21.927592469313154 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3296752389115696 1101 2 0.2927541050400002 1101 3 0.36902090793279285 1101 4 0.24668054043061005 1101 5 0.40718044183196866 1101 6 0.4525043142014553 1101 7 0.4922898595117289 1101 8 0.40545716953194955 1101 9 0.6293415551317878 1101 10-11 0.7320980452734176 1101 12-13 0.6674695367944494 1101 14-15 0.6651256085572257 1101 16-17 0.7005514751465611 1101 18-19 0.8127054806945537 1101 20-21 0.8390637720493004 1101 22-23 0.9053096236080194 1101 24-25 0.6984885892694948 1101 26-27 0.7479302657795941 1101 28-29 0.6835891316844211 1101 30-31 0.7878686166155404 1101 32-33 0.7786539635672156 1101 34-35 0.9787160456633188 1101 36-37 0.8509381977087003 1101 38-39 0.697092731475152 1101 40-41 0.8571538091883255 1101 42-43 0.8838184299896028 1101 44-45 0.6012917354937812 1101 46-47 0.7303346914690749 1101 48-49 0.8373993593793827 1101 50-51 0.646376865241308 1101 52-53 0.7894465916861861 1101 54-55 0.7238278062011148 1101 56-57 0.7105114121686285 1101 58-59 0.6643283601125844 1101 60-61 0.41981688349763147 1101 62-63 0.3104747792903382 1101 64-65 0.16224169359652052 1101 66-67 -2.9006052752415705 1101 68-69 -7.958917125944627 1101 70-71 -11.50388336134009 1101 72-73 -11.51777646373581 1101 74-75 -9.917743753440707 1101 76-77 -4.647988118035872 1101 78-79 -1.620054061498731 1101 80-81 -1.0516007022211724 1101 82-83 -0.6503770640067721 1101 84-85 -0.7003657118936104 1101 86-87 -0.6630398888767068 1101 88-89 -0.6870925747787524 1101 90-91 -0.689231733985249 1101 92-93 -1.3100606309166665 1101 94-95 -5.021460947733825 1101 96-97 -1.7749579856204853 1101 98-99 -0.8754701018412554 1101 100-101 -0.6095712129496498 1102 1 0.40036104335382916 1102 2 0.2639757262185931 1102 3 0.34814403308647 1102 4 0.17380485401146473 1102 5 0.4166366339473697 1102 6 0.5131934995141378 1102 7 0.49379332234264695 1102 8 0.46231982423238804 1102 9 0.6349097462636593 1102 10-11 0.5971219162675965 1102 12-13 0.6568223562354802 1102 14-15 0.6138426706832831 1102 16-17 0.7411744261341866 1102 18-19 0.6830913609502716 1102 20-21 0.878324129806856 1102 22-23 0.6987192314270487 1102 24-25 0.6522897633535862 1102 26-27 0.6954332880728415 1102 28-29 0.6005797984330528 1102 30-31 0.4604902255000525 1102 32-33 0.4134892229721814 1102 34-35 0.4888663407211027 1102 36-37 0.7415119723993087 1102 38-39 0.7763831072640244 1102 40-41 0.7491132284982243 1102 42-43 0.8900973722894108 1102 44-45 0.6030500442389624 1102 46-47 0.5235342755195589 1102 48-49 0.4648671386597627 1102 50-51 0.5174389195811173 1102 52-53 0.538098085416209 1102 54-55 0.35712502569132454 1102 56-57 0.3696351520260137 1102 58-59 0.35514904079450105 1102 60-61 0.12830901529010674 1102 62-63 0.13114521613977814 1102 64-65 0.10997522978207996 1102 66-67 -3.1649509231392834 1102 68-69 -8.012001914076748 1102 70-71 -11.257186113163712 1102 72-73 -9.41000353528732 1102 74-75 -7.946551586815023 1102 76-77 -4.21551844341904 1102 78-79 -1.2523895258049365 1102 80-81 -0.5933275586263846 1102 82-83 -0.3732686302718342 1102 84-85 -0.38294424029292173 1102 86-87 -0.3639468329796465 1102 88-89 -0.31096561797193445 1102 90-91 -0.38254964850045425 1102 92-93 -0.7270743490545222 1102 94-95 -4.73755491544139 1102 96-97 -1.2888054673842753 1102 98-99 -0.4755681983087605 1102 100-101 -0.17093785297915787 1103 1 0.37670739424874355 1103 2 0.18701085945953366 1103 3 0.39858443084489537 1103 4 0.2270940801101986 1103 5 0.429215983436265 1103 6 0.44298102960974006 1103 7 0.37552413239460947 1103 8 0.36057847982070257 1103 9 0.6433689846236987 1103 10-11 0.6675909992890041 1103 12-13 0.5010031028942024 1103 14-15 0.6416149243382847 1103 16-17 0.6372360174197453 1103 18-19 0.6735611248554036 1103 20-21 0.6481970078241517 1103 22-23 0.707603077698792 1103 24-25 0.8450451379277837 1103 26-27 0.7545479602268799 1103 28-29 0.7052387116598666 1103 30-31 0.7578452395386748 1103 32-33 0.7068606174364618 1103 34-35 0.764670260236862 1103 36-37 0.7276625768657539 1103 38-39 0.7001340453904845 1103 40-41 0.7829671140867305 1103 42-43 0.7634568877160675 1103 44-45 0.7316519596719502 1103 46-47 0.7595103866642461 1103 48-49 0.8066737642611415 1103 50-51 0.6992063417498997 1103 52-53 0.8140974329550943 1103 54-55 0.6930486179439654 1103 56-57 0.6891747433014928 1103 58-59 0.8323789889933693 1103 60-61 0.6985897084855139 1103 62-63 0.7647657259049296 1103 64-65 0.5682657717763284 1103 66-67 -2.6723839338938298 1103 68-69 -7.611419304261084 1103 70-71 -11.525154990211043 1103 72-73 -9.522868244318811 1103 74-75 -7.675990269681865 1103 76-77 -3.832165070337208 1103 78-79 -0.8881528855173428 1103 80-81 -0.23650681705495202 1103 82-83 -0.051479289403264517 1103 84-85 -0.09221837595734073 1103 86-87 -0.09234507692922733 1103 88-89 0.09050268036919107 1103 90-91 0.08299419411592268 1103 92-93 0.1741449590093005 1103 94-95 1.0343356315537982 1103 96-97 1.1886555045759089 1103 98-99 0.8146476328407104 1103 100-101 0.7765679673625137 1104 1 0.33196959186436814 1104 2 0.18971660710897353 1104 3 0.3266261786159568 1104 4 0.16913159092873542 1104 5 0.3442267774843586 1104 6 0.4374166221384215 1104 7 0.48328098572981304 1104 8 0.39343358809116324 1104 9 0.5978178667714502 1104 10-11 0.5787523512825814 1104 12-13 0.542183170894468 1104 14-15 0.6221892722088711 1104 16-17 0.6059448904167013 1104 18-19 0.6107787110317204 1104 20-21 0.6856920919194209 1104 22-23 0.5230397476599435 1104 24-25 0.7586094714693914 1104 26-27 0.8479170856459248 1104 28-29 0.8178787819799567 1104 30-31 0.7376306935666008 1104 32-33 0.5278407303884762 1104 34-35 0.5728125763492287 1104 36-37 0.6590903763252953 1104 38-39 0.6094466005746071 1104 40-41 0.839534756160198 1104 42-43 0.8913777595228751 1104 44-45 0.6931828250382708 1104 46-47 0.5962091617845644 1104 48-49 0.6810120700767115 1104 50-51 0.6099685264898795 1104 52-53 0.71397211588949 1104 54-55 0.7070584976659049 1104 56-57 0.7352104597732563 1104 58-59 0.760470200528033 1104 60-61 0.5017953991585458 1104 62-63 0.5817784549789948 1104 64-65 0.2865975168175723 1104 66-67 -2.8799954497494227 1104 68-69 -7.317960013847138 1104 70-71 -9.137359305162072 1104 72-73 -8.609989799434718 1104 74-75 -7.474242758349632 1104 76-77 -3.836441514092943 1104 78-79 -0.785023132358809 1104 80-81 -0.1748119633562233 1104 82-83 0.07064392846631051 1104 84-85 0.03058676371773217 1104 86-87 0.3052516800207776 1104 88-89 0.29593191791231277 1104 90-91 0.11292012567571774 1104 92-93 0.236196341842831 1104 94-95 1.1533270973260024 1104 96-97 1.2716123083055102 1104 98-99 1.0462316553536368 1104 100-101 0.9597095466154997 1105 1 0.4799073967789198 1105 2 0.4156526760683015 1105 3 0.5094664108747935 1105 4 0.3267424825512535 1105 5 0.42990589514022304 1105 6 0.5364839203336302 1105 7 0.6125591426242494 1105 8 0.5598271411126277 1105 9 0.7895529989135568 1105 10-11 0.9020973948311166 1105 12-13 0.8246013864522226 1105 14-15 0.9682392258169656 1105 16-17 0.9453129129242583 1105 18-19 0.9535662660285809 1105 20-21 1.045571697438561 1105 22-23 1.0290837900411987 1105 24-25 1.045984886751782 1105 26-27 1.1658419457219509 1105 28-29 1.122557630263458 1105 30-31 1.1087855901575523 1105 32-33 1.1643049562422334 1105 34-35 1.2055102657329684 1105 36-37 1.1865439095927854 1105 38-39 1.0330449289694457 1105 40-41 1.0521976890266203 1105 42-43 1.2633052310143 1105 44-45 1.0683418295577383 1105 46-47 1.1025590540356163 1105 48-49 0.9194851777358579 1105 50-51 1.0596716556988213 1105 52-53 1.277916251054549 1105 54-55 1.1929080307538058 1105 56-57 1.2471713409201755 1105 58-59 1.3538760025094092 1105 60-61 1.5176659208430223 1105 62-63 1.4573396474006586 1105 64-65 1.4731668727183198 1105 66-67 2.0297423611511753 1105 68-69 -5.215589162929778 1105 70-71 -5.606376806883059 1105 72-73 -1.5845556235644942 1105 74-75 1.2999605354757549 1105 76-77 1.4507477723695992 1105 78-79 1.4027827175109948 1105 80-81 1.3903948547575737 1105 82-83 1.386282864744782 1105 84-85 1.3069955437602339 1105 86-87 1.4220517234196564 1105 88-89 1.4342536906817571 1105 90-91 1.4494513204917965 1105 92-93 1.5487646202541292 1105 94-95 2.4947313130034736 1105 96-97 2.548356868480301 1105 98-99 2.2908329842572925 1105 100-101 2.164825376731333 1106 1 0.17366910489620224 1106 2 0.12087911062531376 1106 3 0.13952897491366656 1106 4 0.06682663437469927 1106 5 0.28000154377423314 1106 6 0.2399597433948628 1106 7 0.2956761237498142 1106 8 0.27180667972224626 1106 9 0.29251538976716773 1106 10-11 0.3470015176408552 1106 12-13 0.4264923659839255 1106 14-15 0.31354485295806 1106 16-17 0.373061452751692 1106 18-19 0.35316236832517944 1106 20-21 0.4573072845952524 1106 22-23 0.5124383834180648 1106 24-25 0.48755245487878796 1106 26-27 0.554296362118663 1106 28-29 0.6322355643219666 1106 30-31 0.580931634150744 1106 32-33 0.5891819455440768 1106 34-35 0.5849066961155955 1106 36-37 0.5546255550597863 1106 38-39 0.5569632326163401 1106 40-41 0.6553079298990312 1106 42-43 0.716966464942054 1106 44-45 0.6674235077988442 1106 46-47 0.6112489135554569 1106 48-49 0.4265260039192711 1106 50-51 0.41165826230263036 1106 52-53 0.6556205889659097 1106 54-55 0.5084555695911206 1106 56-57 0.5050134076896526 1106 58-59 0.7142453623449256 1106 60-61 0.7077127705616846 1106 62-63 0.7337889527621542 1106 64-65 0.7391428898367565 1106 66-67 1.5189708932508275 1106 68-69 -5.431190196866499 1106 70-71 -6.436616027652871 1106 72-73 -2.4786095602518223 1106 74-75 0.4915649097836159 1106 76-77 0.8398914152284682 1106 78-79 0.6638448184000332 1106 80-81 0.8288926105939503 1106 82-83 0.8019483256694251 1106 84-85 0.8273495773899135 1106 86-87 0.8426415149763571 1106 88-89 0.7373003239188414 1106 90-91 0.6739084994004507 1106 92-93 0.757449261791681 1106 94-95 1.7870299808224104 1106 96-97 1.7022852329145373 1106 98-99 1.3660172092187395 1106 100-101 1.2548733476693243 1107 1 0.015780343720464884 1107 2 -0.07877760177068538 1107 3 -0.09904494392094776 1107 4 0.0031354835588430774 1107 5 0.06589131328207998 1107 6 0.09756265445235357 1107 7 0.08255215974753582 1107 8 0.01303429063710837 1107 9 0.09325211411872658 1107 10-11 0.12542775727753508 1107 12-13 0.1395821617030677 1107 14-15 0.15529983226652178 1107 16-17 0.11619667022178248 1107 18-19 0.20752022860164487 1107 20-21 0.18842977621867618 1107 22-23 0.1645896145126784 1107 24-25 0.4049054627241233 1107 26-27 0.3603168002349477 1107 28-29 0.4271471647549845 1107 30-31 0.33032727010540697 1107 32-33 0.2916680057538059 1107 34-35 0.3493455372290697 1107 36-37 0.33349825580513226 1107 38-39 0.2528691902677309 1107 40-41 0.21976629574572826 1107 42-43 0.3471167987013217 1107 44-45 0.25020275996143937 1107 46-47 0.021563723132473456 1107 48-49 0.29650766270582807 1107 50-51 0.19951608480007366 1107 52-53 0.3514261814646744 1107 54-55 0.333547593269536 1107 56-57 0.4056816617438699 1107 58-59 0.30934271520566625 1107 60-61 0.3012817781235029 1107 62-63 0.3196995174481927 1107 64-65 0.29081014826331497 1107 66-67 1.1239159999982924 1107 68-69 -5.776692320181237 1107 70-71 -6.384545971305297 1107 72-73 -2.3718584156812064 1107 74-75 0.27984565672501915 1107 76-77 0.3287435949442745 1107 78-79 0.2889601112810638 1107 80-81 0.321511191587156 1107 82-83 0.5333924404668302 1107 84-85 0.543684423890074 1107 86-87 0.5971871336462833 1107 88-89 0.42762514995146006 1107 90-91 0.21378415627065195 1107 92-93 0.4007067094409926 1107 94-95 1.1729110578120476 1107 96-97 1.3774868826207616 1107 98-99 0.9703545083463432 1107 100-101 0.866953273368356 1108 1 0.11136021210407421 1108 2 0.038803040081919704 1108 3 0.04622569519326092 1108 4 -0.0309180791002035 1108 5 0.07674493640882929 1108 6 0.24459087725933415 1108 7 0.1765849524648715 1108 8 0.09483366203177468 1108 9 0.25228204797459597 1108 10-11 0.22798061799075242 1108 12-13 0.18810453716429976 1108 14-15 0.20736188160691427 1108 16-17 -0.03444302498352414 1108 18-19 0.1890114927939024 1108 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2.0705383214111137 2316 100-101 1.7203875499718073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 41.0 4 120.0 5 261.0 6 571.0 7 1426.0 8 3427.0 9 5801.0 10 9042.0 11 13507.0 12 18348.0 13 23370.0 14 28484.0 15 34757.0 16 41792.0 17 47458.0 18 45932.0 19 41804.0 20 41502.0 21 43018.0 22 45348.0 23 51953.0 24 62963.0 25 78975.0 26 100226.0 27 126306.0 28 153875.0 29 183884.0 30 218744.0 31 253734.0 32 295782.0 33 342639.0 34 398845.0 35 460547.0 36 495819.0 37 422559.0 38 178167.0 39 10119.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.4584609561725 20.52878493711728 15.808410374305815 23.204343732404404 2 15.862175627858122 20.998512737054433 39.387516803548124 23.751794831539318 3 19.260992957499738 24.51516531773005 30.157994931268465 26.065846793501745 4 13.451829531784684 17.84114081496794 36.810529880989925 31.896499772257453 5 13.875400155799442 37.42440398538241 34.235046932537905 14.465148926280236 6 31.844387606133868 36.740221669411255 17.24104504630765 14.174345678147226 7 28.241710755287713 32.60091330600422 20.65912196489261 18.498253973815444 8 25.726989243544374 35.929247982434624 19.368931245109376 18.974831528911622 9 25.499036473844644 17.240484449271808 20.43913434474382 36.82134473213973 10-11 24.810062716793386 27.025682351704567 27.235158777431295 20.929096154070752 12-13 25.665008233768965 24.2027375821917 27.735363161767285 22.396891022272055 14-15 22.61301285869451 25.785384768111374 25.990423133971014 25.61117923922311 16-17 22.02595786323262 28.768370877738953 26.755900106292017 22.44977115273641 18-19 21.876254423952172 27.394032162539588 29.019269236622332 21.71044417688591 20-21 22.803834064620744 26.52017326658956 29.054831898496396 21.621160770293297 22-23 22.381813297828852 26.543662333718743 28.885065928547238 22.189458439905167 24-25 22.176938439438 26.72690748980998 29.022622426217254 22.073531644534764 26-27 22.151611412439525 26.891627233008624 28.891692402634767 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22.636117284084808 27.32200622852851 27.659051761114917 22.382824726271764 54-55 22.567174260710633 27.186157354737944 27.793704244519272 22.45296414003215 56-57 22.539959746712963 27.35270088059761 27.715662762648492 22.391676610040935 58-59 22.60213543783227 27.216547301173318 27.71576695653677 22.465550304457643 60-61 22.65383459054103 27.26044511015859 27.842978726088198 22.24274157321219 62-63 22.546904393533822 27.14852844805003 27.9281923432558 22.37637481516035 64-65 22.471947513500805 27.12054349637703 27.8420658412499 22.565443148872266 66-67 22.040809050204466 27.097460790277907 28.35360743986349 22.508122719654136 68-69 21.956945116379394 26.781077647213408 28.93020721394596 22.331770022461235 70-71 21.627201948834607 26.70109400402456 29.23552409104429 22.43617995609654 72-73 22.36912235663788 26.993976182292073 28.03224882753096 22.604652633539086 74-75 22.137340921148542 27.194588722512965 27.901747493054845 22.76632286328365 76-77 22.441418781756425 27.157305115030695 27.638799060350333 22.76247704286255 78-79 22.327715594219285 27.25789779211056 27.635422007879395 22.77896460579076 80-81 22.32570046948291 27.28929271099694 27.515333117033432 22.869673702486725 82-83 22.434451023673546 27.34913516228116 27.485301846466488 22.731111967578805 84-85 22.383903857608352 27.2627915396564 27.419992291790756 22.93331231094449 86-87 22.25490173178172 27.295101304792645 27.59047823710507 22.85951872632056 88-89 22.381435510034233 27.21665442882405 27.42343445446921 22.978475606672507 90-91 22.243214202289185 27.329163746788133 27.549439383321655 22.878182667601028 92-93 22.36765283148795 27.23809497113987 27.482834644860016 22.911417552512166 94-95 21.866864996872412 27.20946684945258 27.844301424456606 23.079366729218403 96-97 21.772870714895998 27.053139813873052 27.96238832365529 23.21160114757566 98-99 22.087582910930724 27.01769447895112 27.434227547833846 23.46049506228431 100-101 21.956942115593147 27.022896430188265 27.503923293595044 23.51623816062354 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 913.0 1 642.0 2 375.5 3 438.0 4 444.5 5 438.0 6 749.0 7 1501.5 8 2410.5 9 3048.0 10 3148.5 11 2996.0 12 3027.0 13 3234.0 14 3593.0 15 3918.0 16 4187.0 17 4613.0 18 5072.0 19 5605.0 20 6404.5 21 7466.0 22 8865.0 23 11063.0 24 13803.5 25 17402.5 26 22885.0 27 29205.0 28 34845.0 29 41049.5 30 49180.0 31 58248.0 32 67962.0 33 77894.0 34 87805.5 35 97621.0 36 108628.0 37 119191.0 38 124528.5 39 126618.0 40 130080.5 41 135184.5 42 140205.0 43 146189.0 44 152337.5 45 156499.0 46 159209.5 47 162472.0 48 167070.0 49 166953.0 50 161600.5 51 156510.0 52 152381.0 53 148229.5 54 142148.0 55 132096.0 56 120666.0 57 109873.0 58 99071.0 59 87074.0 60 72851.0 61 57765.5 62 44205.0 63 33590.5 64 24479.0 65 17263.5 66 12586.5 67 9261.5 68 6658.5 69 4751.5 70 3433.5 71 2423.5 72 1673.5 73 1271.5 74 935.5 75 615.0 76 396.0 77 221.5 78 129.0 79 83.0 80 48.0 81 31.0 82 26.5 83 17.5 84 9.0 85 5.0 86 3.0 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02242388143372692 2 0.018780000700746296 3 0.0 4 0.0 5 1.1679104913399437E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.5548509162257806E-4 18-19 0.0012029478060801419 20-21 3.503731474019831E-5 22-23 0.0 24-25 0.0 26-27 3.386940424885837E-4 28-29 0.017810634992934143 30-31 0.004753395699753571 32-33 0.008946194363663968 34-35 0.00995059738621632 36-37 0.09565186924074139 38-39 0.09877019025261904 40-41 0.11314716840101374 42-43 0.09778914543989348 44-45 0.09233500344533595 46-47 0.09388832439881807 48-49 0.09663291405346694 50-51 0.09489272742137042 52-53 0.07224694299428892 54-55 0.06149048736904803 56-57 0.03502563563528491 58-59 0.015638321479041845 60-61 0.008572463006435186 62-63 0.001740186632096516 64-65 0.004706679280099973 66-67 0.4193616201254336 68-69 0.8058699181294746 70-71 0.8923770482230241 72-73 0.2767363909229997 74-75 0.1454282143816498 76-77 0.014447052777875105 78-79 0.00852574658678159 80-81 0.0013314179601275357 82-83 0.0 84-85 0.0 86-87 2.3358209826798874E-4 88-89 7.124253997173657E-4 90-91 0.003503731474019831 92-93 0.0016467537927893205 94-95 0.2215759784170141 96-97 0.22681989652313045 98-99 0.013489366174976349 100-101 0.007194328626654053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4281150.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.72329686816508 #Duplication Level Percentage of deduplicated Percentage of total 1 68.24298858109539 32.56780403226415 2 15.580536103439513 14.871090996592157 3 6.451512258407307 9.236623042697344 4 3.208035465000387 6.123921154392618 5 1.811504010431576 4.322547183384885 6 1.1534866323796824 3.3028910994309344 7 0.7457408765683289 2.491244926743721 8 0.5364212256998957 2.0479831520368883 9 0.37331722814124546 1.6034336014126653 >10 1.7383366178463615 14.306822712245626 >50 0.10484371742987178 3.4166861423225425 >100 0.05145672980283859 4.340735540080894 >500 0.001377716483782847 0.40684646481322256 >1k 2.9522484923237997E-4 0.23615685017622312 >5k 4.920414153870729E-5 0.16681498097359257 >10k+ 9.840828307741457E-5 0.5583981204324369 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12458 0.29099657802226037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11322 0.26446165165901686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7104 0.1659367226095792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.3358209826798872E-5 0.0 7 0.0 0.0 0.0 2.3358209826798872E-5 0.0 8 0.0 0.0 0.0 2.3358209826798872E-5 0.0 9 0.0 0.0 0.0 4.6716419653597745E-5 0.0 10-11 0.0 0.0 0.0 7.007462948039662E-5 0.0 12-13 0.0 0.0 0.0 7.007462948039662E-5 9.34328393071955E-5 14-15 0.0 0.0 0.0 7.007462948039662E-5 1.2847015404739381E-4 16-17 0.0 0.0 0.0 7.007462948039662E-5 1.4014925896079324E-4 18-19 0.0 0.0 0.0 9.34328393071955E-5 1.4014925896079324E-4 20-21 0.0 0.0 0.0 1.5182836387419267E-4 2.219029933545893E-4 22-23 0.0 0.0 0.0 2.1022388844118986E-4 2.3358209826798874E-4 24-25 0.0 0.0 0.0 3.6205225231538253E-4 2.3358209826798874E-4 26-27 0.0 0.0 0.0 5.372388260163741E-4 2.3358209826798874E-4 28-29 0.0 0.0 0.0 0.0013664552748677342 2.3358209826798874E-4 30-31 0.0 0.0 0.0 0.0036789180477208227 2.3358209826798874E-4 32-33 0.0 0.0 0.0 0.008887798839096971 2.3358209826798874E-4 34-35 0.0 0.0 0.0 0.017308433481657965 2.3358209826798874E-4 36-37 0.0 0.0 0.0 0.03035399366992514 2.3358209826798874E-4 38-39 0.0 0.0 0.0 0.05022015112761758 2.5694030809478763E-4 40-41 0.0 0.0 0.0 0.07231701762376931 2.5694030809478763E-4 42-43 0.0 1.1679104913399436E-5 0.0 0.09544164535230021 2.6861941300818706E-4 44-45 0.0 2.3358209826798872E-5 0.0 0.12704530324795907 2.919776228349859E-4 46-47 0.0 2.3358209826798872E-5 0.0 0.16581993156044522 3.0365672774838534E-4 48-49 0.0 2.3358209826798872E-5 0.0 0.2094764257267323 3.2701493757518425E-4 50-51 0.0 2.3358209826798872E-5 0.0 0.2551066886233839 3.503731474019831E-4 52-53 0.0 2.3358209826798872E-5 0.0 0.30148441423449307 3.7373135722878196E-4 54-55 0.0 2.3358209826798872E-5 0.0 0.35423893112831834 3.7373135722878196E-4 56-57 0.0 3.503731474019831E-5 0.0 0.41592796328089415 3.7373135722878196E-4 58-59 0.0 4.6716419653597745E-5 0.0 0.47743012975485555 3.7373135722878196E-4 60-61 0.0 4.6716419653597745E-5 0.0 0.5389673335435572 3.7373135722878196E-4 62-63 2.3358209826798872E-5 4.6716419653597745E-5 0.0 0.6017308433481658 3.7373135722878196E-4 64-65 2.3358209826798872E-5 4.6716419653597745E-5 0.0 0.6731602489985167 3.9708956705558087E-4 66-67 2.3358209826798872E-5 4.6716419653597745E-5 0.0 0.7474977517723042 4.204477768823797E-4 68-69 2.3358209826798872E-5 4.6716419653597745E-5 0.0 0.8376137252840943 4.204477768823797E-4 70-71 2.3358209826798872E-5 4.6716419653597745E-5 0.0 0.9538908938018991 4.671641965359775E-4 72-73 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.0823376896394659 5.022015112761758E-4 74-75 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.2249862770517268 5.489179309297735E-4 76-77 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.3893346413930836 5.839552456699718E-4 78-79 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.5773448722889878 6.306716653235696E-4 80-81 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.7834109993810074 6.423507702369691E-4 82-83 2.3358209826798872E-5 4.6716419653597745E-5 0.0 1.9992758954953693 7.124253997173656E-4 84-85 2.3358209826798872E-5 5.839552456699718E-5 0.0 2.216857620032001 7.241045046307651E-4 86-87 2.3358209826798872E-5 7.007462948039662E-5 0.0 2.4478119196944745 7.825000291977623E-4 88-89 2.3358209826798872E-5 7.007462948039662E-5 0.0 2.7048223024187426 8.408955537647594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9880 0.0 43.60288 1 GTATCAA 16100 0.0 35.765034 1 ATCAACG 20155 0.0 28.391567 3 TCAACGC 20305 0.0 28.111845 4 TATCAAC 20540 0.0 27.864931 2 CAACGCA 20790 0.0 27.38768 5 AACGCAG 21330 0.0 26.716526 6 ACGCAGA 24495 0.0 23.05176 7 CGCAGAG 24840 0.0 22.674385 8 GCAGAGT 26315 0.0 20.935415 9 GAGTACT 14065 0.0 20.797813 12-13 GTACATG 16155 0.0 19.860487 1 AGAGTAC 22665 0.0 19.709078 10-11 GTACTTT 14675 0.0 19.6912 14-15 AGTACTT 14620 0.0 19.32785 12-13 TACATGG 16120 0.0 18.987114 2 CAGAGTA 25945 0.0 18.249018 10-11 ACATGGG 15835 0.0 18.188242 3 ACTTTTT 17825 0.0 16.596666 16-17 GTGGTAT 6010 0.0 16.244299 1 >>END_MODULE