FastQCFastQC Report
Fri 27 May 2016
SRR939476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4280156
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85980.2008805286536285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66390.1551111688452477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.038.7802471
GTATCAA153950.036.3192061
ATCAACG190400.029.8330023
TCAACGC193600.029.1366794
TATCAAC191650.029.085962
CAACGCA196450.028.229055
AACGCAG200350.027.8738926
ACGCAGA222050.024.7347347
CGCAGAG224300.024.0539918
GTACATG137800.022.29841
GCAGAGT227300.021.7091879
TACATGG136550.021.5044352
GAGTACT119850.020.72379312-13
AGAGTAC188500.020.33494410-11
ACATGGG146050.019.8086473
GTACTTT129850.019.2756214-15
AGTACTT129300.018.89305512-13
CAGAGTA222150.018.34679610-11
AACGCGG5650.017.1748586
ACTTTTT167150.016.65242816-17
Produced by FastQC (version 0.11.5)