FastQCFastQC Report
Fri 27 May 2016
SRR939476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4280156
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124700.29134452108754916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110530.2582382511291645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.1658350770392481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94300.040.5234451
GTATCAA156000.036.48521
ATCAACG196250.028.651543
TCAACGC198200.028.3490244
TATCAAC200400.028.1825282
CAACGCA203500.027.6289025
AACGCAG210350.026.7291726
ACGCAGA239950.023.3528617
CGCAGAG241600.023.075648
GCAGAGT256550.020.8624489
GTACATG162500.020.0105671
AGAGTAC219400.019.975310-11
GAGTACT133400.019.8991712-13
AGTACTT139550.019.41284412-13
TACATGG160000.019.3400842
GTACTTT138900.019.09416214-15
ACATGGG157950.018.685733
CAGAGTA249700.017.67478810-11
GTGGTAT60400.017.2119221
TATACCG2504.652009E-617.0665555
Produced by FastQC (version 0.11.5)