##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4280156 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.895928325976904 31.0 31.0 31.0 28.0 34.0 2 31.146007762333895 31.0 31.0 34.0 28.0 34.0 3 31.157499866827283 31.0 31.0 34.0 28.0 34.0 4 35.063235779256644 35.0 35.0 37.0 32.0 37.0 5 34.62487301864699 35.0 35.0 37.0 31.0 37.0 6 34.67581508711365 35.0 35.0 37.0 31.0 37.0 7 34.52380287073649 35.0 35.0 37.0 30.0 37.0 8 34.41812097502988 35.0 35.0 37.0 30.0 37.0 9 35.936434092589145 37.0 35.0 39.0 30.0 39.0 10-11 35.734521008112786 37.0 35.0 39.0 30.0 39.0 12-13 35.72291115090198 37.0 35.0 39.0 30.0 39.0 14-15 36.57880752477246 38.0 35.5 40.0 30.5 41.0 16-17 36.481925541966234 38.0 35.0 40.0 30.0 41.0 18-19 36.45994047880498 38.0 35.0 40.0 30.0 41.0 20-21 36.321038298604066 38.0 34.5 40.0 29.5 41.0 22-23 36.15914770396219 38.0 34.0 40.0 29.0 41.0 24-25 36.003938758306944 38.0 34.0 40.0 29.0 41.0 26-27 35.545945171157314 38.0 34.0 40.0 27.0 41.0 28-29 35.34189805231398 38.0 34.0 40.0 27.0 41.0 30-31 35.06580589585987 37.5 33.0 40.0 27.0 41.0 32-33 34.8611704807021 37.0 33.0 40.0 26.0 41.0 34-35 35.15772999862622 38.0 33.5 40.0 27.0 41.0 36-37 35.02863248442347 38.0 33.0 40.0 26.0 41.0 38-39 34.854609388069036 38.0 33.0 40.0 25.0 41.0 40-41 34.716613600065045 38.0 33.0 40.0 25.0 41.0 42-43 34.57830579072352 37.5 33.0 40.0 25.0 41.0 44-45 34.21853444126803 37.0 33.0 40.0 24.0 41.0 46-47 33.875828357657994 37.0 32.0 40.0 24.0 41.0 48-49 33.529694478425554 37.0 31.5 40.0 23.0 41.0 50-51 33.30037117805986 37.0 31.0 39.5 22.0 41.0 52-53 33.19886413018591 37.0 31.0 39.0 22.0 40.0 54-55 32.867311144733975 36.0 31.0 39.0 21.0 40.0 56-57 32.36729829473505 36.0 30.5 39.0 18.5 40.0 58-59 31.90840497402431 35.0 30.0 39.0 17.5 40.0 60-61 31.53720273279759 35.0 29.0 38.5 14.0 40.0 62-63 31.372271477955476 35.0 29.0 38.0 14.0 40.0 64-65 30.910202572055788 34.0 29.0 38.0 12.0 40.0 66-67 30.039633368503388 34.0 27.5 37.5 7.5 39.5 68-69 26.573316252959003 31.0 19.5 36.0 5.5 39.0 70-71 25.24620095622683 30.0 14.5 35.0 2.0 38.0 72-73 26.248110933339813 31.0 20.5 35.0 2.0 38.0 74-75 26.99226687064677 31.0 23.0 35.0 2.0 37.5 76-77 26.674074029077445 30.5 23.5 34.0 2.0 36.5 78-79 27.79842580504075 32.0 26.0 35.0 2.0 37.0 80-81 27.812800982020285 32.0 26.0 35.0 2.0 36.5 82-83 27.48935190679966 32.0 26.0 35.0 2.0 36.0 84-85 27.11566681214423 32.0 25.5 35.0 2.0 36.0 86-87 26.665706693868167 32.0 25.0 34.0 2.0 35.0 88-89 26.177529276970276 31.5 24.5 34.0 2.0 35.0 90-91 25.58094144232126 31.0 22.5 34.0 2.0 35.0 92-93 25.00599475813498 31.0 20.0 34.0 2.0 35.0 94-95 24.15328308127087 31.0 17.5 34.0 2.0 35.0 96-97 23.474616228941187 30.0 5.0 34.0 2.0 35.0 98-99 22.61326584825413 30.0 2.0 34.0 2.0 35.0 100-101 21.16454949305586 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23101771352099476 1101 2 0.21284419081191075 1101 3 0.35824433871476913 1101 4 0.2772460470690419 1101 5 0.49710704168820996 1101 6 0.34275632497581654 1101 7 0.5327789013731206 1101 8 0.5865103882940019 1101 9 0.6256720251248353 1101 10-11 0.7520424191062247 1101 12-13 0.7203785536980334 1101 14-15 0.7480800609190368 1101 16-17 0.7036351138234451 1101 18-19 0.5582142722712646 1101 20-21 0.6381496353228115 1101 22-23 0.632400931153704 1101 24-25 0.7399317104749557 1101 26-27 0.7553913520263436 1101 28-29 0.6123498953672097 1101 30-31 0.48314612179093075 1101 32-33 0.8735852660008234 1101 34-35 0.651494292301166 1101 36-37 0.7744222247072301 1101 38-39 0.8168893728993041 1101 40-41 0.7937416484491919 1101 42-43 0.5115425936611118 1101 44-45 0.664046376780334 1101 46-47 0.5966752277293637 1101 48-49 0.6962884189591776 1101 50-51 0.5376587888815223 1101 52-53 0.2523766756520587 1101 54-55 0.30629490957274896 1101 56-57 0.39402194614213926 1101 58-59 0.4523134445577348 1101 60-61 0.4604527811621004 1101 62-63 0.0639295798183035 1101 64-65 0.07162837532447952 1101 66-67 -0.1282374769632426 1101 68-69 -7.600730992368074 1101 70-71 -8.151918419872057 1101 72-73 -5.203614944036737 1101 74-75 -3.1926223559672486 1101 76-77 -0.46009461030975274 1101 78-79 -0.08370967116879413 1101 80-81 -0.13649968130200563 1101 82-83 -0.4064000674171062 1101 84-85 -0.5068117045493103 1101 86-87 -0.42524827185811986 1101 88-89 -0.362686220304564 1101 90-91 -0.3875873499720157 1101 92-93 -0.5707927672317865 1101 94-95 -0.5448926120428972 1101 96-97 -0.7291821359291575 1101 98-99 -0.6303060843266657 1101 100-101 -0.6305122494001836 1102 1 0.3317738527032006 1102 2 0.28912387998755307 1102 3 0.36999169751145544 1102 4 0.2965162601403506 1102 5 0.4746818571130902 1102 6 0.467964538005063 1102 7 0.46532273191340323 1102 8 0.589190558641107 1102 9 0.5804214226506446 1102 10-11 0.5846413490531788 1102 12-13 0.5450302984715663 1102 14-15 0.6695211079665597 1102 16-17 0.6427350401517273 1102 18-19 0.49073446954450617 1102 20-21 0.5897931618947112 1102 22-23 0.6086362185743965 1102 24-25 0.5226700418743704 1102 26-27 0.5563470553442471 1102 28-29 0.37975067754941705 1102 30-31 0.4833094420597277 1102 32-33 0.6117925559397293 1102 34-35 0.45465065208529865 1102 36-37 0.6058633799384694 1102 38-39 0.8691500481133616 1102 40-41 0.753070537025863 1102 42-43 0.447379155829573 1102 44-45 0.48444325130166277 1102 46-47 0.6413934316642198 1102 48-49 0.5065615262003007 1102 50-51 0.4267699909247398 1102 52-53 0.2337946828179014 1102 54-55 0.016797417368827894 1102 56-57 0.12141539133248358 1102 58-59 0.24049898864081243 1102 60-61 0.48065130803417233 1102 62-63 0.233025048327395 1102 64-65 0.0536998260732382 1102 66-67 -0.2190037320194982 1102 68-69 -7.137382567947842 1102 70-71 -7.9213801250879605 1102 72-73 -1.7007869845167605 1102 74-75 -0.048327563601748835 1102 76-77 0.4365893358812478 1102 78-79 0.07252806965431091 1102 80-81 -0.06065873978235814 1102 82-83 -0.11157777728109863 1102 84-85 -0.24728641841614518 1102 86-87 -0.4046996569045369 1102 88-89 -0.38206596358599754 1102 90-91 -0.5236978351704167 1102 92-93 -0.40733979926721275 1102 94-95 -0.06278032542309475 1102 96-97 -0.432150808571123 1102 98-99 -0.46532814382241483 1102 100-101 -0.47035997594257495 1103 1 0.40809051689826603 1103 2 0.3026255057928253 1103 3 0.36563881562350176 1103 4 0.3394598908946449 1103 5 0.4008321152501111 1103 6 0.45042161572822437 1103 7 0.45904722534402964 1103 8 0.4564155983684728 1103 9 0.5095712697020929 1103 10-11 0.6153048433459603 1103 12-13 0.5241684326737399 1103 14-15 0.6822463599609208 1103 16-17 0.6742155942776336 1103 18-19 0.6833218579407756 1103 20-21 0.6383880834068023 1103 22-23 0.6246032446774095 1103 24-25 0.5620629255505989 1103 26-27 0.6974559032433163 1103 28-29 0.6358447212793266 1103 30-31 0.5572003470004532 1103 32-33 0.6118546640272484 1103 34-35 0.6746054444852589 1103 36-37 0.7768309521565016 1103 38-39 0.7773270117284667 1103 40-41 0.7441539147026432 1103 42-43 0.47679530891188193 1103 44-45 0.7101670763093324 1103 46-47 0.7322943651301443 1103 48-49 0.861730077645241 1103 50-51 0.5909387039324869 1103 52-53 0.5160269856022381 1103 54-55 0.3891962606954209 1103 56-57 0.5700126117979281 1103 58-59 0.6914336676524009 1103 60-61 0.7193419114394608 1103 62-63 0.29679401605553934 1103 64-65 0.3682134320606245 1103 66-67 0.2932942372323204 1103 68-69 -7.180863703990632 1103 70-71 -8.020144910174896 1103 72-73 -1.1836223445637515 1103 74-75 0.40403303440962546 1103 76-77 0.7199369490769847 1103 78-79 0.5227483693578634 1103 80-81 0.4790468197229103 1103 82-83 0.3679390254327224 1103 84-85 0.11821542856115741 1103 86-87 0.15275741173185153 1103 88-89 0.2753585017119846 1103 90-91 0.1328377166438095 1103 92-93 0.1695078731742754 1103 94-95 0.4166886354354524 1103 96-97 0.47620932165322216 1103 98-99 0.45888763139555877 1103 100-101 0.5247758067847066 1104 1 0.2596260605828249 1104 2 0.24722824500763352 1104 3 0.2107653536995464 1104 4 0.1857762387503996 1104 5 0.4335344908712102 1104 6 0.363953486156646 1104 7 0.477083499414789 1104 8 0.54050690730584 1104 9 0.5626944703930263 1104 10-11 0.5141799419747528 1104 12-13 0.41548190163499754 1104 14-15 0.5261197792345911 1104 16-17 0.6264086854639501 1104 18-19 0.4441948160581859 1104 20-21 0.4795995915210156 1104 22-23 0.4989479629722595 1104 24-25 0.4634034774453397 1104 26-27 0.708370112187076 1104 28-29 0.48941535015783444 1104 30-31 0.530091290211594 1104 32-33 0.5628309557978781 1104 34-35 0.3419504506695574 1104 36-37 0.6685595950243055 1104 38-39 0.6368476891216801 1104 40-41 0.6284014303233079 1104 42-43 0.5958021436206877 1104 44-45 0.7812985618189003 1104 46-47 0.9010915470123066 1104 48-49 0.8749298755895012 1104 50-51 0.712972376335621 1104 52-53 0.5696592574765091 1104 54-55 0.5162665990188415 1104 56-57 0.6082144756155472 1104 58-59 0.6941780138234783 1104 60-61 0.8278058612827159 1104 62-63 0.45423915712136775 1104 64-65 0.3662894375411838 1104 66-67 0.30582855738671455 1104 68-69 -6.5103709731992545 1104 70-71 -4.549852369177305 1104 72-73 0.331962870713582 1104 74-75 1.1702668006433932 1104 76-77 1.2973995367766413 1104 78-79 0.7351750641737205 1104 80-81 0.4733487411184285 1104 82-83 0.4011376771504622 1104 84-85 0.37613672011853083 1104 86-87 0.3597975083347542 1104 88-89 0.3754940496615724 1104 90-91 0.3848905283641102 1104 92-93 0.3501029913155769 1104 94-95 0.441413874277071 1104 96-97 0.2861243142960497 1104 98-99 0.5841403343576239 1104 100-101 0.7685999783028414 1105 1 0.07655396026934369 1105 2 0.19686251877983807 1105 3 0.1574738176493895 1105 4 0.1464867925644029 1105 5 0.2784665702860494 1105 6 0.18046341301139535 1105 7 0.2733217439288893 1105 8 0.3668391957070938 1105 9 0.35496197300535925 1105 10-11 0.2898330245243841 1105 12-13 0.284029446044606 1105 14-15 0.35192960159613307 1105 16-17 0.25420387877638717 1105 18-19 0.4156681702901679 1105 20-21 0.47228506696511374 1105 22-23 0.5319678166817638 1105 24-25 0.46225405215798077 1105 26-27 0.5263429439530185 1105 28-29 0.34531921640652996 1105 30-31 0.3872490749555837 1105 32-33 0.5732802765920226 1105 34-35 0.4332775144104133 1105 36-37 0.5742022282531494 1105 38-39 0.5128142512951328 1105 40-41 0.5697807406681363 1105 42-43 0.3482577340073121 1105 44-45 0.43114182200699247 1105 46-47 0.5379776494156516 1105 48-49 0.7632266363000539 1105 50-51 0.41182295104826494 1105 52-53 0.2978724236207029 1105 54-55 0.31417673486001263 1105 56-57 0.4775979656886946 1105 58-59 0.6090160493511654 1105 60-61 0.7267609292032979 1105 62-63 0.5210103169959801 1105 64-65 0.36628943754118737 1105 66-67 0.38952761694784144 1105 68-69 -3.3553030526140937 1105 70-71 -3.560615169595277 1105 72-73 0.7545334036289439 1105 74-75 1.086045275042558 1105 76-77 1.0975040299845809 1105 78-79 0.6921238625018269 1105 80-81 0.5228785216826886 1105 82-83 0.44993600525913635 1105 84-85 0.40518583192626423 1105 86-87 0.5461088980944204 1105 88-89 0.42805413325613983 1105 90-91 0.30077349597121383 1105 92-93 0.17821166425183677 1105 94-95 0.2492508648726819 1105 96-97 0.2609414511821555 1105 98-99 0.4152793103241841 1105 100-101 0.451776571824265 1106 1 0.06428694367193444 1106 2 0.19157710651768056 1106 3 0.19886041821802536 1106 4 0.20490821610210475 1106 5 0.2990298615790863 1106 6 0.28738426548157037 1106 7 0.2545445574692664 1106 8 0.3917085791971644 1106 9 0.38041943454943095 1106 10-11 0.40170515399875484 1106 12-13 0.24915423037548834 1106 14-15 0.24109268390276384 1106 16-17 0.2395833349256904 1106 18-19 0.29220579999539353 1106 20-21 0.3232656020675435 1106 22-23 0.29108910170521796 1106 24-25 0.3468012077556537 1106 26-27 0.46100309801974504 1106 28-29 0.45188163287271976 1106 30-31 0.35205551951343494 1106 32-33 0.6639439299661802 1106 34-35 0.48853255074002533 1106 36-37 0.5135572864534339 1106 38-39 0.5658712851460805 1106 40-41 0.4556571470738149 1106 42-43 0.4372908073226611 1106 44-45 0.5018812937087631 1106 46-47 0.6068775546427361 1106 48-49 0.6979937136493319 1106 50-51 0.5368818803573916 1106 52-53 0.3850306943796227 1106 54-55 0.3241291053977875 1106 56-57 0.4880096203264941 1106 58-59 0.7712223140834915 1106 60-61 0.8155716506924513 1106 62-63 0.451815157704587 1106 64-65 0.5802332056148636 1106 66-67 0.7628891391465764 1106 68-69 -3.1016895316790034 1106 70-71 -3.2939472878913065 1106 72-73 0.7718926415575496 1106 74-75 1.3296395983882832 1106 76-77 1.3226504419794303 1106 78-79 0.9809479494012976 1106 80-81 0.7752441993089967 1106 82-83 0.6252974788563748 1106 84-85 0.4502345549220621 1106 86-87 0.5203124411431936 1106 88-89 0.511542991364081 1106 90-91 0.7382768373237951 1106 92-93 0.6501406574719297 1106 94-95 0.9902304279452672 1106 96-97 0.8381048250977301 1106 98-99 0.6688964763711809 1106 100-101 0.8006891134392937 1107 1 0.015483169616295811 1107 2 0.05098922120660632 1107 3 0.08108724274605095 1107 4 -0.18223627934796838 1107 5 0.2530692428276211 1107 6 0.23460308827337428 1107 7 -0.012655790145998935 1107 8 0.0011564246178110693 1107 9 0.1805836892564514 1107 10-11 0.049533393287539695 1107 12-13 0.08123214980349758 1107 14-15 -0.011379530294277629 1107 16-17 -0.015261390582992362 1107 18-19 0.19896672221462808 1107 20-21 0.14293322510901874 1107 22-23 0.13317610105180933 1107 24-25 0.1730627159032352 1107 26-27 0.4578550837534223 1107 28-29 0.3169959874449333 1107 30-31 0.26541132386761745 1107 32-33 0.1236367430575882 1107 34-35 0.10540668442629197 1107 36-37 0.31961398168768085 1107 38-39 0.36136121353158046 1107 40-41 0.1865713483354483 1107 42-43 -0.12899575175454459 1107 44-45 0.11316371859423668 1107 46-47 0.28851552929224056 1107 48-49 0.26159075261275433 1107 50-51 0.24600705941681866 1107 52-53 -0.11728660935757063 1107 54-55 -0.0313640134800508 1107 56-57 0.3145352947469533 1107 58-59 0.37926970495172796 1107 60-61 0.3837785569026444 1107 62-63 0.047643983153587754 1107 64-65 -0.1236560880110602 1107 66-67 0.24897645939734403 1107 68-69 -2.873800802881515 1107 70-71 -2.9464668029921235 1107 72-73 0.9219890983024968 1107 74-75 1.2552169824349875 1107 76-77 0.7561413367873229 1107 78-79 0.8648259000850054 1107 80-81 0.5901375380896603 1107 82-83 0.518142382439045 1107 84-85 0.3428782441699596 1107 86-87 0.2129501250312522 1107 88-89 0.3113934602226962 1107 90-91 0.40107787627423264 1107 92-93 0.2597014453936879 1107 94-95 0.41174795687186005 1107 96-97 0.5955208184607663 1107 98-99 0.6379285282982998 1107 100-101 0.8169903497086999 1108 1 -0.012677776805624319 1108 2 0.03622015526228495 1108 3 0.05892991718719287 1108 4 0.05106557978313475 1108 5 -0.09101408413140177 1108 6 -0.21426654226204533 1108 7 0.1666493143172758 1108 8 0.22673758852371861 1108 9 0.21159164711901468 1108 10-11 0.29947284729334456 1108 12-13 0.09912144262963096 1108 14-15 0.1250502431129661 1108 16-17 0.31769528113168377 1108 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100-101 0.7059490803288959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 47.0 4 109.0 5 285.0 6 686.0 7 1747.0 8 3917.0 9 6399.0 10 10104.0 11 14732.0 12 19610.0 13 25049.0 14 31264.0 15 37712.0 16 45152.0 17 50534.0 18 48570.0 19 46451.0 20 47282.0 21 48344.0 22 50916.0 23 57341.0 24 68442.0 25 84151.0 26 105840.0 27 132742.0 28 161241.0 29 193937.0 30 225979.0 31 263888.0 32 304595.0 33 350952.0 34 401755.0 35 449987.0 36 459648.0 37 372548.0 38 150216.0 39 7975.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.25247018277553 20.658000326226304 15.92631678817584 23.163212702822324 2 15.875537717263011 21.10868425793791 39.28632601561936 23.72945200917972 3 19.188764853486102 24.6699874303177 30.201465372628 25.9397823435682 4 13.39990800245358 17.94520416603429 36.88060968135034 31.774278150161788 5 13.833327570303513 37.541879314679186 34.195085412774674 14.429707702242627 6 31.72578289202543 36.88547800594184 17.24420792139352 14.14453118063921 7 28.154347645272743 32.65925354122607 20.675508088957507 18.510890724543685 8 25.759131209236298 35.87785118112517 19.404503013441566 18.95851459619696 9 25.43026469128695 17.271706919093603 20.436357927140975 36.86167046247847 10-11 24.76629432486987 27.094016634699024 27.23208225336922 20.90760678706188 12-13 25.621636220735876 24.231091109763288 27.773193313514742 22.37407935598609 14-15 22.531082979218514 25.820308885937806 26.03826823134484 25.610339903498847 16-17 22.033353414492055 28.810808733329807 26.739342003861328 22.41649584831681 18-19 21.82940508407297 27.42948788489004 29.06785297995328 21.673254051083713 20-21 22.793304730014512 26.551742506581537 29.022598709019015 21.632354054384933 22-23 22.37082812518253 26.548684041447146 28.87908134060722 22.201406492763105 24-25 22.143821428120827 26.727709473032956 29.06706585651128 22.061403242334933 26-27 22.19430952463562 26.902883321692013 28.86290141977422 22.039905733898145 28-29 22.06360454409625 27.027503777362366 28.919666185603475 21.9892254929379 30-31 22.315931328083593 26.76476061519828 28.853683464487272 22.065624592230854 32-33 22.22402772251597 26.745898665251133 28.81497085019127 22.21510276204163 34-35 22.424802530635876 26.912223299317223 28.750200641070123 21.912773528976775 36-37 22.373489995110553 26.746900954716164 28.755338111785665 22.124270938387617 38-39 22.241395015393387 26.97932390237708 28.786504848136396 21.99277623409314 40-41 22.48558092771905 26.802996337874212 28.674368918986058 22.037053815420677 42-43 22.262258241129356 26.893008389988132 28.50186399364277 22.342869375239744 44-45 22.4678600004957 26.921268400035732 28.403197001092884 22.207674598375686 46-47 22.47278588036356 27.055532329597852 28.10883730543974 22.362844484598853 48-49 22.50802046702306 27.107886752630595 28.090181344445725 22.29391143590062 50-51 22.49103351850813 27.2276387408114 27.967601425284776 22.313726315395694 52-53 22.56897260685468 27.31835131046726 27.720618951696324 22.392057130981737 54-55 22.551414155155722 27.197388000566608 27.772537915178276 22.4786599290994 56-57 22.528018576339775 27.364976069711755 27.730190630198727 22.376814723749742 58-59 22.635925843918894 27.21064073046065 27.72226464301008 22.43116878261037 60-61 22.629531597852836 27.22432749303236 27.89215820861867 22.253982700496135 62-63 22.515261984650284 27.160573438013646 27.922159847984112 22.402004729351958 64-65 22.456612570744976 27.101513529237558 27.8684828316566 22.573391068360866 66-67 22.324917888900316 27.21558403329441 27.90994682390066 22.549551253904614 68-69 22.302041381066232 26.795804595828148 28.555015823888745 22.34713819921687 70-71 21.862139875261835 26.657294118686863 29.002984406256356 22.477581599794952 72-73 22.339048531016665 27.027893608248682 28.089283555923615 22.543774304811038 74-75 22.373120618760694 27.19379434710922 27.655590425413173 22.777494608716914 76-77 21.83523106268669 27.221916412671774 28.13660233663986 22.806250188001673 78-79 22.344765156743456 27.220969960079106 27.642260464995644 22.792004418181794 80-81 22.291810308673288 27.273507854039238 27.568225621006597 22.866456216280877 82-83 22.438488670378195 27.339068343377722 27.479649647185788 22.7427933390583 84-85 22.375281678541263 27.262125465375586 27.43626676199054 22.926326094092605 86-87 22.26678540316731 27.285131500908104 27.55804788501484 22.89003521090974 88-89 22.371951281483064 27.205065910834424 27.4466118327713 22.97637097491121 90-91 22.255699792989684 27.317767840036634 27.49762849359109 22.928903873382588 92-93 22.332662769452195 27.265769933504362 27.47907700503273 22.92249029201071 94-95 22.1520185242057 27.17077174565294 27.56299774245888 23.114211987682484 96-97 22.112236251516695 27.051671839082005 27.598050449133616 23.23804146026768 98-99 22.047105642159707 27.012834279704368 27.434416376228256 23.50564370190767 100-101 21.97922971980369 26.973079063361748 27.547510301429483 23.50018091540508 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 960.0 1 676.0 2 420.0 3 443.0 4 446.5 5 491.5 6 713.0 7 1415.0 8 2372.5 9 2960.5 10 3053.5 11 2997.5 12 3011.0 13 3191.5 14 3470.5 15 3793.0 16 4180.5 17 4562.0 18 5066.5 19 5611.0 20 6393.5 21 7481.5 22 8855.5 23 11015.5 24 13814.0 25 17354.0 26 22531.0 27 28885.5 28 34791.0 29 41269.5 30 49304.0 31 58380.5 32 67998.0 33 77708.5 34 87611.0 35 97798.5 36 109436.5 37 119648.5 38 124804.5 39 127193.0 40 130623.0 41 135350.5 42 140185.5 43 145612.0 44 151771.5 45 156682.0 46 159190.5 47 161895.5 48 166645.5 49 166730.5 50 160902.5 51 155790.5 52 151912.5 53 147784.0 54 141723.0 55 131962.5 56 120694.5 57 109615.0 58 98908.0 59 87227.5 60 73162.0 61 58140.0 62 44375.5 63 33694.0 64 24756.5 65 17521.0 66 12669.5 67 9418.0 68 6884.5 69 4880.0 70 3475.5 71 2477.0 72 1778.0 73 1370.5 74 1013.0 75 673.5 76 431.0 77 231.0 78 116.0 79 74.0 80 53.0 81 39.0 82 33.0 83 23.0 84 14.0 85 7.5 86 4.5 87 2.0 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02144781638800081 2 0.016891907678131358 3 3.7381815055339103E-4 4 0.09240317408991634 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00717263576374319 12-13 0.0 14-15 0.0 16-17 5.724090430348801E-4 18-19 1.168181720479347E-4 20-21 0.0 22-23 1.4018180645752163E-4 24-25 0.003597999699076389 26-27 0.0033760451721853127 28-29 0.0012382726237081078 30-31 1.7522725807190203E-4 32-33 5.840908602396734E-4 34-35 0.0029204543011983677 36-37 0.08403899297128423 38-39 0.09652685556320845 40-41 0.07636403906773492 42-43 0.08063958416468932 44-45 0.07938962972377642 46-47 0.07784762985274368 48-49 0.07311649388480233 50-51 0.07280108482027291 52-53 0.05623626802387576 54-55 0.047988905077291574 56-57 0.02720695226996399 58-59 0.021926770893397344 60-61 0.01293177164570637 62-63 0.0017989998495381945 64-65 0.006039499494878225 66-67 0.15245939633975958 68-69 0.7104998042127436 70-71 0.8707276089936908 72-73 0.3547183794235537 74-75 0.05106122300215226 76-77 0.1928083929651162 78-79 0.01325886252744059 80-81 0.0030139088388367154 82-83 0.0016471362258758792 84-85 2.5699997850545635E-4 86-87 0.002114408914067618 88-89 4.0886360216777146E-4 90-91 0.0041119996560873015 92-93 0.0041119996560873015 94-95 0.07377067564827076 96-97 0.035734678829463226 98-99 0.013142044355392653 100-101 0.01241777168869546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4280156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.72091397705932 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24534203105318 33.73696352406992 2 15.243875984770192 14.853911410618975 3 6.099818272679474 8.915661640167336 4 3.109142853658565 6.059211260619507 5 1.7607076531658172 4.289164305432088 6 1.1389842495926588 3.329541218737763 7 0.7301011965118277 2.4899838312860587 8 0.5091413298443386 1.9844664746809282 9 0.36410428897534 1.5965544367661295 >10 1.651616639822578 14.024426716945126 >50 0.09840799530501214 3.3099813895347245 >100 0.04701765247049955 4.077735141837431 >500 0.0014015476618131541 0.4294635397981862 >1k 1.9331685065737867E-4 0.18191975007281536 >5k 4.8329212664344635E-5 0.16713258944044043 >10k+ 9.665842532868927E-5 0.5538827699926008 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12470 0.29134452108754916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11053 0.2582382511291645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7098 0.1658350770392481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.336363440958694E-5 2 0.0 0.0 0.0 0.0 2.336363440958694E-5 3 0.0 0.0 0.0 0.0 2.336363440958694E-5 4 0.0 0.0 0.0 0.0 2.336363440958694E-5 5 0.0 0.0 0.0 0.0 4.672726881917388E-5 6 0.0 0.0 0.0 0.0 4.672726881917388E-5 7 0.0 0.0 0.0 0.0 4.672726881917388E-5 8 0.0 0.0 0.0 0.0 4.672726881917388E-5 9 0.0 0.0 0.0 0.0 4.672726881917388E-5 10-11 0.0 0.0 0.0 0.0 7.009090322876082E-5 12-13 0.0 0.0 0.0 0.0 1.6354544086710857E-4 14-15 0.0 0.0 0.0 1.168181720479347E-5 1.6354544086710857E-4 16-17 0.0 0.0 0.0 1.0513635484314123E-4 1.6354544086710857E-4 18-19 0.0 0.0 0.0 1.6354544086710857E-4 1.6354544086710857E-4 20-21 0.0 0.0 0.0 2.8036361291504326E-4 1.8690907527669552E-4 22-23 0.0 0.0 0.0 4.322272365773584E-4 1.8690907527669552E-4 24-25 0.0 0.0 0.0 5.957726774444669E-4 1.8690907527669552E-4 26-27 0.0 0.0 0.0 7.826817527211624E-4 1.8690907527669552E-4 28-29 0.0 0.0 0.0 0.0014835907850087706 1.8690907527669552E-4 30-31 0.0 0.0 0.0 0.0037965905915578776 1.98590892481489E-4 32-33 0.0 0.0 0.0 0.00851604474229444 2.1027270968628246E-4 34-35 0.0 0.0 0.0 0.016950316764155322 2.453181613006629E-4 36-37 0.0 2.336363440958694E-5 0.0 0.030664770162582858 2.5699997850545635E-4 38-39 0.0 2.336363440958694E-5 0.0 0.05075749575482763 2.8036361291504326E-4 40-41 0.0 2.336363440958694E-5 0.0 0.0728244484546825 2.8036361291504326E-4 42-43 0.0 2.336363440958694E-5 0.0 0.09771840091809739 2.8036361291504326E-4 44-45 0.0 2.336363440958694E-5 0.0 0.13008871639258007 3.0372724732463023E-4 46-47 0.0 2.336363440958694E-5 0.0 0.16917607675981905 3.2709088173421715E-4 48-49 0.0 2.336363440958694E-5 0.0 0.21058811875081188 3.387726989390106E-4 50-51 0.0 2.336363440958694E-5 0.0 0.2551192059354846 3.504545161438041E-4 52-53 0.0 2.336363440958694E-5 0.0 0.30083015665784146 4.0886360216777146E-4 54-55 0.0 2.336363440958694E-5 0.0 0.35554778844509405 4.205454193725649E-4 56-57 0.0 2.336363440958694E-5 0.0 0.4195758285445671 4.205454193725649E-4 58-59 1.168181720479347E-5 2.336363440958694E-5 0.0 0.4835337777408113 4.205454193725649E-4 60-61 2.336363440958694E-5 3.504545161438041E-5 0.0 0.5502135903457724 4.205454193725649E-4 62-63 2.336363440958694E-5 4.672726881917388E-5 0.0 0.6154331758001343 4.439090537821519E-4 64-65 3.504545161438041E-5 4.672726881917388E-5 0.0 0.6899398059323072 4.5559087098694535E-4 66-67 4.672726881917388E-5 4.672726881917388E-5 0.0 0.7702756628496719 4.906363226013258E-4 68-69 4.672726881917388E-5 4.672726881917388E-5 0.0 0.8615924279395424 4.906363226013258E-4 70-71 4.672726881917388E-5 4.672726881917388E-5 0.0 0.9756770547615554 4.906363226013258E-4 72-73 4.672726881917388E-5 4.672726881917388E-5 0.0 1.1048545894121617 4.906363226013258E-4 74-75 4.672726881917388E-5 4.672726881917388E-5 0.0 1.2431088960308923 5.023181398061192E-4 76-77 4.672726881917388E-5 4.672726881917388E-5 0.0 1.4087921094464781 5.139999570109127E-4 78-79 4.672726881917388E-5 4.672726881917388E-5 0.0 1.5967408664544003 5.139999570109127E-4 80-81 4.672726881917388E-5 4.672726881917388E-5 0.0 1.8041865763771228 5.256817742157062E-4 82-83 4.672726881917388E-5 4.672726881917388E-5 0.0 2.0247276033864186 5.840908602396735E-4 84-85 4.672726881917388E-5 4.672726881917388E-5 0.0 2.2494857664066448 5.840908602396735E-4 86-87 4.672726881917388E-5 4.672726881917388E-5 0.0 2.4878882919220704 6.541817634684343E-4 88-89 4.672726881917388E-5 4.672726881917388E-5 0.0 2.748147497427664 7.242726666971952E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9430 0.0 40.523445 1 GTATCAA 15600 0.0 36.4852 1 ATCAACG 19625 0.0 28.65154 3 TCAACGC 19820 0.0 28.349024 4 TATCAAC 20040 0.0 28.182528 2 CAACGCA 20350 0.0 27.628902 5 AACGCAG 21035 0.0 26.729172 6 ACGCAGA 23995 0.0 23.352861 7 CGCAGAG 24160 0.0 23.07564 8 GCAGAGT 25655 0.0 20.862448 9 GTACATG 16250 0.0 20.010567 1 AGAGTAC 21940 0.0 19.9753 10-11 GAGTACT 13340 0.0 19.89917 12-13 AGTACTT 13955 0.0 19.412844 12-13 TACATGG 16000 0.0 19.340084 2 GTACTTT 13890 0.0 19.094162 14-15 ACATGGG 15795 0.0 18.68573 3 CAGAGTA 24970 0.0 17.674788 10-11 GTGGTAT 6040 0.0 17.211922 1 TATACCG 250 4.652009E-6 17.066555 5 >>END_MODULE