FastQCFastQC Report
Fri 27 May 2016
SRR939475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316510
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124860.3764800950396652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97910.29521997521490967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67970.2049443541554224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA203600.030.0137651
GGTATCA139400.028.0495821
GTACATG128950.027.6014251
TACATGG134650.025.9332732
ACATGGG137250.024.7615363
TATCAAC247950.024.443612
CAACGCA254250.023.8601865
TCAACGC255550.023.8033354
ATCAACG256400.023.630493
AACGCAG257250.023.3606836
GAGTACT132550.022.9638312-13
CATGGGG61550.022.1004054
CATGGGA66700.021.902014
AGTACTT145700.021.20380212-13
ACGCAGA279650.021.1191487
GTACTTT146900.021.04206314-15
CGCAGAG281350.020.861348
ATGGGAT29400.020.0587355
AGAGTAC218250.020.01407410-11
GCAGAGT277350.019.6994619
Produced by FastQC (version 0.11.5)