FastQCFastQC Report
Fri 27 May 2016
SRR939475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316510
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174820.5271203765403994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153940.4641626287874905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113490.34219706860525073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48850.1472933897380077No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37810.1140053851789984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.10508034047839446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149750.028.7107561
GTATCAA214600.027.3460791
GTACATG144600.024.9799081
TACATGG152450.023.24922
ACATGGG148150.022.9926513
TCAACGC261300.022.2649044
CAACGCA262550.022.1408545
TATCAAC265400.022.0852662
ATCAACG263650.022.0844173
AGGTAAG9350.021.791682
GAGTACT144950.021.73528112-13
AACGCAG268550.021.6814616
GTACTTT152800.020.4791214-15
GTAAGGT14200.020.3518244
CATGGGA73150.020.3365444
GGTAAGG12200.020.1932093
TAAGGTG14700.019.9818735
AGTACTT155950.019.9135712-13
CATGGGG67000.019.586954
ACGCAGA298800.019.4389027
Produced by FastQC (version 0.11.5)