##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3316510 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.995737386590122 31.0 31.0 31.0 30.0 34.0 2 31.25819943253601 31.0 31.0 34.0 30.0 34.0 3 31.24570979734721 31.0 31.0 34.0 28.0 34.0 4 35.18862750300768 35.0 35.0 37.0 33.0 37.0 5 34.81488884399565 35.0 35.0 37.0 32.0 37.0 6 34.77191234158799 35.0 35.0 37.0 32.0 37.0 7 34.633590732426555 35.0 35.0 37.0 30.0 37.0 8 34.64467195937898 35.0 35.0 37.0 30.0 37.0 9 35.987836309855844 37.0 35.0 39.0 30.0 39.0 10-11 35.8450969543285 37.0 35.0 39.0 30.0 39.0 12-13 35.82535014216752 37.0 35.0 39.0 30.0 39.0 14-15 36.67436718116333 38.0 36.0 40.0 31.0 41.0 16-17 36.68938537800278 38.0 36.0 40.0 31.0 41.0 18-19 36.658571058130384 38.0 35.0 40.0 30.0 41.0 20-21 36.56310398581641 38.0 35.0 40.0 30.0 41.0 22-23 36.421744544717185 38.0 35.0 40.0 30.0 41.0 24-25 36.19940479600544 38.0 34.0 40.0 29.5 41.0 26-27 35.809473211297416 38.0 34.0 40.0 28.0 41.0 28-29 35.53149455300904 38.0 34.0 40.0 27.0 41.0 30-31 35.30360017005829 38.0 34.0 40.0 27.0 41.0 32-33 35.07959813177105 37.5 33.0 40.0 27.0 41.0 34-35 35.02433205387591 38.0 33.0 40.0 26.0 41.0 36-37 35.020129141778554 38.0 33.0 40.0 26.0 41.0 38-39 34.973432765165796 38.0 33.0 40.0 26.0 41.0 40-41 34.75553865358464 38.0 33.0 40.0 25.5 41.0 42-43 34.447861909055 37.5 33.0 40.0 24.5 41.0 44-45 34.257397836882745 37.0 33.0 40.0 24.0 41.0 46-47 33.92052669824605 37.0 33.0 40.0 23.5 41.0 48-49 33.595400285239606 37.0 32.0 40.0 22.5 41.0 50-51 33.32982608223705 37.0 32.0 40.0 22.0 41.0 52-53 32.96126605980383 36.5 31.0 39.5 20.0 41.0 54-55 32.726456727101684 36.0 31.0 39.0 19.5 40.5 56-57 32.31748374043799 36.0 31.0 39.0 18.0 40.0 58-59 31.879157759210734 35.0 30.0 39.0 15.5 40.0 60-61 31.580770297692453 35.0 30.0 38.5 13.5 40.0 62-63 31.179893170833196 35.0 29.0 38.0 12.5 40.0 64-65 30.73528438026721 34.5 29.0 38.0 8.0 40.0 66-67 29.05847788790023 33.5 25.5 37.0 6.5 39.5 68-69 25.430827134548064 30.0 16.0 35.0 2.0 38.0 70-71 24.828874328737136 29.5 15.0 35.0 2.0 38.0 72-73 26.625817350166287 31.0 21.5 35.0 2.0 38.0 74-75 27.23451987179294 32.0 24.0 35.0 2.0 37.5 76-77 27.253501270914306 31.5 25.5 34.5 2.0 36.0 78-79 28.09847942566131 33.0 26.0 35.0 2.0 37.0 80-81 27.964169714549328 33.0 26.0 35.0 2.0 36.5 82-83 27.567088143862073 33.0 26.0 35.0 2.0 36.0 84-85 27.22989844746435 32.5 26.0 35.0 2.0 36.0 86-87 26.811328927095047 32.0 25.0 35.0 2.0 35.0 88-89 26.324430953019892 32.0 24.5 34.0 2.0 35.0 90-91 25.88415442739506 32.0 23.5 34.0 2.0 35.0 92-93 25.343997153634394 31.5 21.5 34.0 2.0 35.0 94-95 23.99574175865594 31.0 12.0 34.0 2.0 35.0 96-97 23.201590979674414 30.0 4.5 34.0 2.0 35.0 98-99 22.887002903654746 30.0 2.0 34.0 2.0 35.0 100-101 21.616813306759212 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4064142255380183 1101 2 0.31307582489558783 1101 3 0.38664011046023816 1101 4 0.3076406349982861 1101 5 0.3947407385018238 1101 6 0.5279317146915758 1101 7 0.5256671343818695 1101 8 0.5927320253960957 1101 9 0.6825406786436332 1101 10-11 0.7655960185712942 1101 12-13 0.6562241372973077 1101 14-15 0.8386879910707066 1101 16-17 0.8450742815724297 1101 18-19 0.8840462838120828 1101 20-21 0.8241822841618998 1101 22-23 0.9168435614055852 1101 24-25 0.7856386962426214 1101 26-27 0.8534851683804234 1101 28-29 0.7721220550419332 1101 30-31 0.9285447763235268 1101 32-33 0.8659522337314272 1101 34-35 0.7807770253736166 1101 36-37 0.7791640225950047 1101 38-39 0.6158114677951403 1101 40-41 0.8096977225231399 1101 42-43 0.9111568751089933 1101 44-45 0.6541433253913311 1101 46-47 0.8219257592986509 1101 48-49 0.7662008419618047 1101 50-51 0.6682625148169379 1101 52-53 0.7178427141627992 1101 54-55 0.7857346725401229 1101 56-57 0.697392100567825 1101 58-59 0.5813073259695898 1101 60-61 0.33091359636109274 1101 62-63 0.46474616171133576 1101 64-65 0.22773174577411837 1101 66-67 -2.6972794320124898 1101 68-69 -7.735940210266257 1101 70-71 -11.299262728430033 1101 72-73 -11.231731096248698 1101 74-75 -9.746871126626509 1101 76-77 -4.766845551303863 1101 78-79 -1.7673934400815554 1101 80-81 -1.0719315293376965 1101 82-83 -0.877779466299895 1101 84-85 -0.9382796999722949 1101 86-87 -0.7651302026940812 1101 88-89 -0.8398337827460907 1101 90-91 -1.0454105463642485 1101 92-93 -1.5720416543207953 1101 94-95 -4.891344085360718 1101 96-97 -1.8782408639938275 1101 98-99 -1.0716022349520564 1101 100-101 -0.8768743007931299 1102 1 0.3361951092826523 1102 2 0.19818037829152502 1102 3 0.28523946803467126 1102 4 0.12429933959172246 1102 5 0.3881774877920563 1102 6 0.4570123909673214 1102 7 0.4397706959309673 1102 8 0.4866935890562871 1102 9 0.6028271312897715 1102 10-11 0.5684574739472907 1102 12-13 0.556409312371926 1102 14-15 0.4448878300093355 1102 16-17 0.6423678591366766 1102 18-19 0.6707310812569389 1102 20-21 0.8348510073183348 1102 22-23 0.7121385448444641 1102 24-25 0.6671971697084516 1102 26-27 0.6787008268211352 1102 28-29 0.5611152850048029 1102 30-31 0.3401413084994047 1102 32-33 0.13223444600485124 1102 34-35 0.38915016907311184 1102 36-37 0.6869799349121024 1102 38-39 0.5956797068575241 1102 40-41 0.6673646303268868 1102 42-43 0.7996773854096375 1102 44-45 0.5225217240591675 1102 46-47 0.5211184050728406 1102 48-49 0.30908013238183685 1102 50-51 0.3271088331542771 1102 52-53 0.37729665243170984 1102 54-55 0.4000072575295661 1102 56-57 0.27041796138168905 1102 58-59 0.2205918540688785 1102 60-61 0.13893060789927247 1102 62-63 0.1311832931810919 1102 64-65 0.0048292970953660586 1102 66-67 -3.035621250641011 1102 68-69 -7.760321102200447 1102 70-71 -11.345564117925555 1102 72-73 -9.766229280896003 1102 74-75 -8.10675296945973 1102 76-77 -4.360604423328322 1102 78-79 -1.5412511100604434 1102 80-81 -0.8634779686985468 1102 82-83 -0.7007037538080816 1102 84-85 -0.7959615082273714 1102 86-87 -0.7522033218248936 1102 88-89 -0.6350949842456899 1102 90-91 -0.7016638449374426 1102 92-93 -1.188099791104662 1102 94-95 -4.755164538334441 1102 96-97 -1.5136004312210218 1102 98-99 -0.7495731119630484 1102 100-101 -0.673416713619801 1103 1 0.33824495589757575 1103 2 0.24743987120188393 1103 3 0.29366173113601945 1103 4 0.20723661958663087 1103 5 0.45854708750649564 1103 6 0.37476362087529225 1103 7 0.2444519006046164 1103 8 0.31571164184337164 1103 9 0.6532480566894918 1103 10-11 0.5343477391196814 1103 12-13 0.4675082598817184 1103 14-15 0.5947379128867567 1103 16-17 0.489946963777804 1103 18-19 0.5427096559420406 1103 20-21 0.7170497682214005 1103 22-23 0.7273702526140937 1103 24-25 0.7924467738760015 1103 26-27 0.6505799353499029 1103 28-29 0.7488246874391677 1103 30-31 0.7915010769020796 1103 32-33 0.7508644592935099 1103 34-35 0.8622222971673281 1103 36-37 0.8517020084026399 1103 38-39 0.7688316641076156 1103 40-41 0.7913395965750993 1103 42-43 0.8845317579445222 1103 44-45 0.7169323357263266 1103 46-47 0.7661318439379272 1103 48-49 0.6129459266267148 1103 50-51 0.4081831383269616 1103 52-53 0.6034375864549233 1103 54-55 0.6284370396803496 1103 56-57 0.6196283286576474 1103 58-59 0.5885829469551993 1103 60-61 0.46910269340614974 1103 62-63 0.5011746431817983 1103 64-65 0.39154556209812696 1103 66-67 -2.813797253828877 1103 68-69 -7.780598569261535 1103 70-71 -11.542887588139163 1103 72-73 -9.202186925940321 1103 74-75 -7.495267157579104 1103 76-77 -3.9278268016451854 1103 78-79 -1.0268782173381084 1103 80-81 -0.3778239130063348 1103 82-83 -0.1608356729239624 1103 84-85 -0.38042402430806277 1103 86-87 -0.23299186335903244 1103 88-89 -0.1859760206905463 1103 90-91 -0.4026843283831667 1103 92-93 -0.31107623023835274 1103 94-95 0.48212519776548035 1103 96-97 0.672329271409847 1103 98-99 0.3323716049107226 1103 100-101 0.3045697450227749 1104 1 0.3716407704153468 1104 2 0.25040147213351105 1104 3 0.3307031587211178 1104 4 0.21025446440038564 1104 5 0.39157050262780757 1104 6 0.5314988798405125 1104 7 0.5277698570047917 1104 8 0.3450110269463096 1104 9 0.6139992730535795 1104 10-11 0.6269280894541751 1104 12-13 0.5926228117243326 1104 14-15 0.7413430357670237 1104 16-17 0.6438456202340461 1104 18-19 0.6598191169922458 1104 20-21 0.7882265813204583 1104 22-23 0.594593986090203 1104 24-25 0.8280774534715434 1104 26-27 0.9001846958897701 1104 28-29 0.9413366437708461 1104 30-31 0.8778102343795453 1104 32-33 0.6536904980477019 1104 34-35 0.8354931132624515 1104 36-37 1.0212333274118066 1104 38-39 0.9143880549088621 1104 40-41 0.8814372121589855 1104 42-43 0.9418708094058701 1104 44-45 0.7756595655168326 1104 46-47 0.6397823783449468 1104 48-49 0.7220161900175412 1104 50-51 0.7903845455056242 1104 52-53 0.8162212079480184 1104 54-55 0.7654808828680828 1104 56-57 0.8266971030277475 1104 58-59 0.8549627698230324 1104 60-61 0.6081807624383018 1104 62-63 0.790529298715061 1104 64-65 0.6322076895950737 1104 66-67 -2.5305867737188414 1104 68-69 -7.175977341161946 1104 70-71 -8.979672096555014 1104 72-73 -8.02756946545976 1104 74-75 -6.83179189857783 1104 76-77 -3.53349643733986 1104 78-79 -0.620174451341498 1104 80-81 0.1793334274512226 1104 82-83 0.3134708237852273 1104 84-85 0.354451184578469 1104 86-87 0.5691492258614375 1104 88-89 0.5216312167433586 1104 90-91 0.3781581040205211 1104 92-93 0.47482180609302915 1104 94-95 1.375799527661087 1104 96-97 1.5565316628564503 1104 98-99 1.2357465262684357 1104 100-101 1.0093120777377855 1105 1 0.4377319265774524 1105 2 0.3636391750318104 1105 3 0.47790858771652367 1105 4 0.27018090720738996 1105 5 0.38871268703915973 1105 6 0.4317956274444299 1105 7 0.6066314179869892 1105 8 0.5903309459786428 1105 9 0.7703651461665117 1105 10-11 0.8293378084948984 1105 12-13 0.8570406968911541 1105 14-15 0.9489957582045179 1105 16-17 0.9640379640801555 1105 18-19 0.9402203778684068 1105 20-21 1.0617401510711062 1105 22-23 1.0692540259169476 1105 24-25 1.164854042206528 1105 26-27 1.1525141591047898 1105 28-29 1.2151119165074107 1105 30-31 1.0388393779949396 1105 32-33 1.1941277526669367 1105 34-35 1.314907320569617 1105 36-37 1.1918989743774375 1105 38-39 1.145485517276967 1105 40-41 1.2676482136136968 1105 42-43 1.3611368071827314 1105 44-45 1.151537880381781 1105 46-47 1.1891643336521014 1105 48-49 1.106529060701078 1105 50-51 1.1579504595039367 1105 52-53 1.4803109148691433 1105 54-55 1.2888310388348856 1105 56-57 1.3771433560827475 1105 58-59 1.5031624073302652 1105 60-61 1.5351854879860127 1105 62-63 1.5357245173149927 1105 64-65 1.4888455965552616 1105 66-67 2.0205628540233533 1105 68-69 -5.1919185903200535 1105 70-71 -4.976253713097535 1105 72-73 -1.32507268244618 1105 74-75 1.3729017804317216 1105 76-77 1.4240214545803447 1105 78-79 1.3336479856101278 1105 80-81 1.3219435213963528 1105 82-83 1.4447642439756727 1105 84-85 1.4107281319732117 1105 86-87 1.4738512644725716 1105 88-89 1.4958977712185764 1105 90-91 1.3682769454308463 1105 92-93 1.4807882618227346 1105 94-95 2.2800451831323336 1105 96-97 2.4157233733441004 1105 98-99 2.122242046215579 1105 100-101 1.9856142499565337 1106 1 0.14609404756687283 1106 2 0.11266648230560605 1106 3 0.1614220107587947 1106 4 0.05477948919712361 1106 5 0.1952402752217992 1106 6 0.31845528825909497 1106 7 0.2866052428565311 1106 8 0.1665607326434042 1106 9 0.26355358334223666 1106 10-11 0.3340474323586875 1106 12-13 0.3362682943738591 1106 14-15 0.3594276053173502 1106 16-17 0.28072535785920394 1106 18-19 0.2751811855832855 1106 20-21 0.4126397854935533 1106 22-23 0.49575939663007773 1106 24-25 0.537543291890934 1106 26-27 0.4698671528303251 1106 28-29 0.6285299786969603 1106 30-31 0.5369131354170165 1106 32-33 0.6130671406054304 1106 34-35 0.5733190003341164 1106 36-37 0.7376833393576874 1106 38-39 0.556929919768443 1106 40-41 0.6213417175635811 1106 42-43 0.6798635405223408 1106 44-45 0.6537063426647407 1106 46-47 0.49796522986135017 1106 48-49 0.5610579860978362 1106 50-51 0.523271646133324 1106 52-53 0.7242864469017078 1106 54-55 0.7435496693844499 1106 56-57 0.7473295465744414 1106 58-59 0.7331389606095016 1106 60-61 0.7561864168188208 1106 62-63 0.7845226235052856 1106 64-65 0.7083610456617677 1106 66-67 1.47033360159881 1106 68-69 -5.268491115117044 1106 70-71 -6.061585753223348 1106 72-73 -2.316972606138723 1106 74-75 0.49857317458159756 1106 76-77 0.7194616145414336 1106 78-79 0.47722467841965255 1106 80-81 0.4297992803504478 1106 82-83 0.46195619551911804 1106 84-85 0.6246794955766362 1106 86-87 0.702197812990299 1106 88-89 0.5485770548731423 1106 90-91 0.6102510936022689 1106 92-93 0.5910876343765175 1106 94-95 1.6232992561639428 1106 96-97 1.6965595003635983 1106 98-99 1.4469112852151689 1106 100-101 1.167234545393427 1107 1 -0.04723363460815122 1107 2 -0.004058970774813986 1107 3 -0.00691246597627071 1107 4 -0.06381431823486139 1107 5 0.08507950455705782 1107 6 0.18652528599623963 1107 7 0.05067177806193257 1107 8 -0.048978276512187335 1107 9 0.15643958918256118 1107 10-11 0.15781405508520407 1107 12-13 0.12252310893325102 1107 14-15 0.01609115307142872 1107 16-17 0.10288717734061237 1107 18-19 0.21499096184770394 1107 20-21 0.09671100206102068 1107 22-23 0.11803334131817422 1107 24-25 0.3095542242482381 1107 26-27 0.4195796262058309 1107 28-29 0.3420214905853527 1107 30-31 0.26234017501224827 1107 32-33 0.29183111734468525 1107 34-35 0.2998442443695737 1107 36-37 0.2122742290200108 1107 38-39 0.10762063019806334 1107 40-41 0.3313617181143371 1107 42-43 0.3207182123660459 1107 44-45 0.2422080314271966 1107 46-47 -0.025202872941846977 1107 48-49 0.11563446650377074 1107 50-51 0.028975484730494827 1107 52-53 0.1400413853795044 1107 54-55 -0.011284046479623555 1107 56-57 0.1994825241482019 1107 58-59 0.18021502496087294 1107 60-61 -0.06722236905616086 1107 62-63 0.15202301561425458 1107 64-65 0.3067780029679845 1107 66-67 1.1550393761966156 1107 68-69 -5.66482094521114 1107 70-71 -6.228701549288367 1107 72-73 -2.390041364691786 1107 74-75 0.07035500754437507 1107 76-77 0.1416387591979138 1107 78-79 0.1909891699890096 1107 80-81 0.1506971620041817 1107 82-83 0.2727502970029043 1107 84-85 0.2666614058836565 1107 86-87 0.32720634360532586 1107 88-89 0.31172123603586854 1107 90-91 0.11501416998749292 1107 92-93 0.19385550466806478 1107 94-95 1.149648979053648 1107 96-97 1.3407095338275568 1107 98-99 0.950154864505592 1107 100-101 0.8209879561428401 1108 1 0.07926276474140792 1108 2 0.0022724955545854186 1108 3 0.029832236292524072 1108 4 -0.049181997557582235 1108 5 0.013836957516303983 1108 6 0.18611230816798496 1108 7 0.1415191569432821 1108 8 0.053942251066985136 1108 9 0.05868128445654719 1108 10-11 0.1896688417563439 1108 12-13 0.20724615567717564 1108 14-15 0.16408433893727192 1108 16-17 0.041174093686798585 1108 18-19 0.13780863213653305 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1.3314023449466887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 33.0 4 90.0 5 211.0 6 620.0 7 1457.0 8 3333.0 9 5634.0 10 8672.0 11 12914.0 12 17026.0 13 21385.0 14 25405.0 15 31674.0 16 37955.0 17 43365.0 18 39957.0 19 34833.0 20 34159.0 21 34421.0 22 36400.0 23 41837.0 24 50209.0 25 63043.0 26 78686.0 27 97845.0 28 119013.0 29 143428.0 30 168288.0 31 195988.0 32 226941.0 33 263055.0 34 306120.0 35 352741.0 36 372476.0 37 311326.0 38 128214.0 39 7750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67224655414001 20.895866295022678 15.362916870493812 23.068970280343503 2 15.722946104216087 21.70789506857528 38.65496673922576 23.914192087982876 3 18.309578442398784 25.8535629321184 31.122625892881373 24.71423273260144 4 12.737244874883539 17.879276709553118 37.160810611154496 32.22266780440885 5 12.71195869630307 38.66139887538304 34.62543573705709 14.001206691256796 6 30.876734880944124 37.75797449728781 17.058594727590147 14.306695894177915 7 27.336296287362316 33.79621951991702 21.449083524548396 17.418400668172264 8 26.073221549158603 35.11582959195058 19.88575339739666 18.92519546149416 9 26.179477824580662 16.295714470934808 20.954708413362237 36.57009929112229 10-11 24.54826006856605 26.951660028162134 27.515520833647418 20.984559069624396 12-13 25.436045722762785 24.706589155467647 28.118730231478274 21.7386348902913 14-15 22.723420101251012 25.993589646948145 26.309071885807672 24.973918365993168 16-17 21.166077264389475 29.17038482337757 27.359542625642035 22.303995286590922 18-19 21.177434404345462 27.482535289366854 29.996244504893525 21.343785801394162 20-21 21.900377776752602 26.617340703733955 30.07335424085989 21.408927278653547 22-23 21.83469972953496 26.362968904058786 30.126699452134925 21.675631914271325 24-25 21.814512846335454 26.796632604756205 30.01786516549023 21.370989383418113 26-27 21.438927663898436 26.80801055933255 30.251628412202937 21.501433364566083 28-29 21.248389955918494 26.83190672344758 30.504722243081297 21.414981077552632 30-31 21.4950545937331 26.79502122884409 29.993829078593837 21.716095098828973 32-33 21.335706319447574 27.02763134663595 29.79224359816182 21.844418735754658 34-35 21.877824694245106 26.857066607562473 29.70094333008411 21.564165368108313 36-37 22.071894439441568 26.471815540370354 29.695585107996497 21.76070491219158 38-39 21.579462323350025 26.970083279165628 29.721188881361332 21.72926551612301 40-41 21.500489053651 26.84743035513748 30.15448963327014 21.497590957941387 42-43 21.558830032319673 26.863271101170465 29.384567902631982 22.193330963877877 44-45 21.55069403090263 26.968478329436273 29.463074911995452 22.01775272766565 46-47 22.096664853682523 27.17069135061685 28.897121097883637 21.835522697816995 48-49 21.823429087049444 27.39945829625161 28.621770381539392 22.15534223515955 50-51 21.85659404441606 27.41140844024646 28.60656371052595 22.12543380481153 52-53 22.05965958813085 27.524889343812127 28.42213389308204 21.993317174974983 54-55 21.99841292829815 27.402195952061987 28.413546833701435 22.185844285938426 56-57 21.951015355435484 27.682329771905106 28.206574902654197 22.160079970005214 58-59 21.889564387551037 27.23942030523948 28.43164470060418 22.439370606605305 60-61 21.91379449682045 27.638870125734577 28.508858956296862 21.938476421148113 62-63 21.76604254210549 27.515142779951468 28.812621516135412 21.90619316180763 64-65 21.75879529570693 27.515500933636783 28.477994893455342 22.247708877200946 66-67 21.168041237737715 27.53981249958366 28.83660660702488 22.455539655653748 68-69 21.198039294064397 27.157275809727444 29.405907803778025 22.23877709243013 70-71 21.04333045009281 27.157113688617933 29.554239656615483 22.24531620467377 72-73 21.71464441105276 27.40994260873325 28.249698650872084 22.625714329341903 74-75 21.343437566009506 27.959736069172454 28.235528413511563 22.46129795130648 76-77 21.638847616098005 27.647252890745605 28.21750095672122 22.496398536435173 78-79 21.534157515301697 27.6042660048685 28.250665102635757 22.61091137719405 80-81 21.731099344210193 27.883188062930977 27.75405937477291 22.63165321808592 82-83 21.833101664098706 27.76296468275386 27.763597878492753 22.640335774654684 84-85 21.437414631645918 27.770050444593863 27.787795001371922 23.004739922388293 86-87 21.629928159247303 27.76467494969097 27.87265015971647 22.732746731345255 88-89 21.640659651793154 27.735515729492473 27.639042875919234 22.98478174279514 90-91 21.48473089150824 27.647343772308737 27.8970584089214 22.970866927261625 92-93 21.600169578993913 27.763012149894685 27.655276419941394 22.98154185117001 94-95 20.955712702654434 27.27707747897265 28.327772477647535 23.439437340725377 96-97 21.029586937932272 27.19358145032374 28.22712437435959 23.5497072373844 98-99 21.309862531607777 27.58947055745793 27.446604152248096 23.6540627586862 100-101 21.058160963870826 27.360106819336945 27.813344184936405 23.768388031855828 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3764.0 1 2514.0 2 1398.5 3 1636.5 4 1574.0 5 1616.5 6 2610.0 7 4624.5 8 6675.5 9 7616.5 10 6996.5 11 5997.0 12 5680.0 13 5570.0 14 5477.5 15 5478.5 16 5602.5 17 5766.0 18 6008.5 19 6573.5 20 7199.5 21 8082.5 22 9399.0 23 10740.0 24 12215.0 25 15001.0 26 19796.0 27 24517.5 28 27100.0 29 31195.5 30 38362.5 31 47592.0 32 60391.0 33 71840.5 34 79952.0 35 87898.5 36 98825.5 37 107017.0 38 108576.5 39 107618.5 40 103885.0 41 99867.0 42 96945.0 43 99033.0 44 103134.0 45 105254.5 46 105874.0 47 107754.0 48 112593.0 49 110296.0 50 103113.5 51 99518.0 52 99923.0 53 103354.0 54 104576.0 55 100317.0 56 93270.0 57 85641.0 58 79629.5 59 72573.5 60 61997.5 61 50809.5 62 40219.0 63 30432.0 64 22549.5 65 16540.0 66 11667.0 67 8613.5 68 6503.5 69 4519.0 70 3174.0 71 2268.0 72 1652.5 73 1327.0 74 1016.0 75 740.0 76 489.5 77 306.5 78 198.5 79 116.5 80 71.5 81 41.0 82 26.0 83 20.0 84 16.0 85 11.0 86 5.0 87 4.0 88 2.0 89 1.5 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023880525009724075 2 0.017850089401207896 3 0.0 4 0.0 5 9.045653412774272E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.372065816174231E-4 18-19 0.001100554498554203 20-21 3.0152178042580904E-5 22-23 0.0 24-25 0.0 26-27 3.769022255322613E-4 28-29 0.01830237207184661 30-31 0.0045077506173658455 32-33 0.008879816433540078 34-35 0.010025599199158151 36-37 0.10301491628247766 38-39 0.10542709052588414 40-41 0.12030719038989782 42-43 0.10590952537456544 44-45 0.10037660070375183 46-47 0.10167314435958281 48-49 0.10385917726766992 50-51 0.10203497049609378 52-53 0.07966205438849876 54-55 0.06723935703495543 56-57 0.03820280957995001 58-59 0.015784665205291103 60-61 0.009588392617540728 62-63 0.0023518698873213105 64-65 0.0053520116025581104 66-67 0.41923588350404495 68-69 0.8073697953571676 70-71 0.8954895356866104 72-73 0.2804001797069811 74-75 0.14956987918022258 76-77 0.01498563248716271 78-79 0.008819512077454916 80-81 0.0015377610801716262 82-83 0.0 84-85 0.0 86-87 2.4121742434064723E-4 88-89 9.950218754051698E-4 90-91 0.0037539461663013227 92-93 0.0018543589496187258 94-95 0.22213109563969355 96-97 0.22917162921263617 98-99 0.014322284570225932 100-101 0.007734033667922003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3316510.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.69709799161363 #Duplication Level Percentage of deduplicated Percentage of total 1 60.913456719286394 15.652990663234185 2 13.386126710200447 6.879692196003551 3 6.64620157217029 5.123642792162297 4 4.12027164038481 4.235160963801405 5 2.812502409648586 3.6136575011194596 6 1.9733446169895055 3.0425537996401557 7 1.5510669837403517 2.790054419091269 8 1.1683948355247478 2.401948526509979 9 0.9086196403953339 2.101399914270927 >10 5.843215095062455 27.483960636200166 >50 0.39455082795504387 7.003692183221699 >100 0.25685465252698936 12.61091792019895 >500 0.019075527598151405 3.3390224551295393 >1k 0.005961102374408303 2.366402112674772 >5k 0.0 0.0 >10k+ 3.576661424644982E-4 1.3549039167416501 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17482 0.5271203765403994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15394 0.4641626287874905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11349 0.34219706860525073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4885 0.1472933897380077 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3781 0.1140053851789984 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3485 0.10508034047839446 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4121742434064723E-4 2 0.0 0.0 0.0 3.0152178042580904E-5 2.4121742434064723E-4 3 0.0 0.0 0.0 3.0152178042580904E-5 2.4121742434064723E-4 4 0.0 0.0 0.0 3.0152178042580904E-5 2.4121742434064723E-4 5 0.0 0.0 0.0 3.0152178042580904E-5 2.7136960238322813E-4 6 0.0 0.0 0.0 3.0152178042580904E-5 3.6182613651097086E-4 7 0.0 0.0 0.0 3.0152178042580904E-5 3.6182613651097086E-4 8 0.0 0.0 0.0 3.0152178042580904E-5 3.6182613651097086E-4 9 0.0 0.0 0.0 3.0152178042580904E-5 3.6182613651097086E-4 10-11 0.0 0.0 0.0 3.0152178042580904E-5 3.769022255322613E-4 12-13 0.0 0.0 0.0 6.030435608516181E-5 0.0012513153887671077 14-15 0.0 0.0 0.0 9.045653412774272E-5 0.0014774567240864644 16-17 0.0 0.0 0.0 1.5076089021290453E-4 0.001628217614299369 18-19 0.0 0.0 0.0 1.5076089021290453E-4 0.0017488263264696926 20-21 0.0 0.0 0.0 3.769022255322613E-4 0.001899587216682597 22-23 0.0 0.0 0.0 9.799457863838795E-4 0.0020201959288529206 24-25 0.0 0.0 0.0 0.0014925328131077547 0.0020201959288529206 26-27 0.0 0.0 0.0 0.0022764894422148585 0.0021106524629806635 28-29 0.0 0.0 0.0 0.0046886636856213314 0.0021860329080871156 30-31 0.0 0.0 0.0 0.011864882059755586 0.0022463372641722775 32-33 0.0 0.0 0.0 0.025734883959342803 0.0023518698873213105 34-35 0.0 0.0 0.0 0.04685648467817073 0.0024724785994916345 36-37 0.0 0.0 0.0 0.08083798933215941 0.002487554688512925 38-39 0.0 0.0 0.0 0.13000111563058758 0.0026081634006832484 40-41 0.0 0.0 0.0 0.18735055826757646 0.002623239489704539 42-43 0.0 0.0 0.0 0.24658451203222664 0.0026533916677471195 44-45 0.0 0.0 0.0 0.31445706480607627 0.002789076468938734 46-47 0.0 0.0 0.0 0.3981293588742383 0.0029549134481729284 48-49 0.0 0.0 0.0 0.48843513211176814 0.003196130872513576 50-51 0.0 0.0 0.0 0.5904097982517766 0.0033468917627264803 52-53 0.0 0.0 0.0 0.6899722901483789 0.0036484135431522897 54-55 0.0 0.0 0.0 0.7974949570482224 0.0036785657211948707 56-57 0.0 0.0 0.0 0.9204103108388034 0.0037690222553226132 58-59 0.0 0.0 0.0 1.0388631422790824 0.0038594787894503557 60-61 0.0 0.0 0.0 1.1565923214463396 0.0038594787894503557 62-63 1.5076089021290452E-5 0.0 0.0 1.2784221968273877 0.003980087501620679 64-65 3.0152178042580904E-5 0.0 0.0 1.4089509755737206 0.004040391857705841 66-67 4.522826706387136E-5 0.0 0.0 1.5475906902135077 0.004040391857705841 68-69 9.045653412774272E-5 0.0 0.0 1.712040669257744 0.004040391857705841 70-71 1.2060871217032362E-4 0.0 0.0 1.9255934702443231 0.004100696213791003 72-73 1.2060871217032362E-4 0.0 0.0 2.158895947848793 0.004356989727152941 74-75 1.2060871217032362E-4 0.0 0.0 2.4186117334185635 0.0046585115075787505 76-77 1.2060871217032362E-4 0.0 0.0 2.703037228894229 0.0047791202197490735 78-79 1.2060871217032362E-4 0.0 0.0 3.0307612520390412 0.004944957198983269 80-81 1.2060871217032362E-4 0.0 0.0 3.388788214116647 0.005095718089196173 82-83 1.2060871217032362E-4 0.0 0.0 3.7533581988294924 0.005698761650047791 84-85 1.2060871217032362E-4 0.0 0.0 4.125752673744389 0.005728913828090372 86-87 1.2060871217032362E-4 0.0 0.0 4.513027248523297 0.006015359519494891 88-89 1.2060871217032362E-4 0.0 0.0 4.926353305130997 0.0063168812999207 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14975 0.0 28.710756 1 GTATCAA 21460 0.0 27.346079 1 GTACATG 14460 0.0 24.979908 1 TACATGG 15245 0.0 23.2492 2 ACATGGG 14815 0.0 22.992651 3 TCAACGC 26130 0.0 22.264904 4 CAACGCA 26255 0.0 22.140854 5 TATCAAC 26540 0.0 22.085266 2 ATCAACG 26365 0.0 22.084417 3 AGGTAAG 935 0.0 21.79168 2 GAGTACT 14495 0.0 21.735281 12-13 AACGCAG 26855 0.0 21.681461 6 GTACTTT 15280 0.0 20.47912 14-15 GTAAGGT 1420 0.0 20.351824 4 CATGGGA 7315 0.0 20.336544 4 GGTAAGG 1220 0.0 20.193209 3 TAAGGTG 1470 0.0 19.981873 5 AGTACTT 15595 0.0 19.91357 12-13 CATGGGG 6700 0.0 19.58695 4 ACGCAGA 29880 0.0 19.438902 7 >>END_MODULE