FastQCFastQC Report
Fri 27 May 2016
SRR939474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327383
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122820.3691189141736914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.27405922311919007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.2072499619070002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34260.10296380068059494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA196150.030.1272011
GTACATG121250.029.8741951
TACATGG130950.027.2508032
GGTATCA136600.026.775341
ACATGGG132700.025.936353
TATCAAC238100.024.3621862
TCAACGC243950.023.9685234
CAACGCA242550.023.883095
ATCAACG243300.023.8604663
AACGCAG247450.023.523516
GAGTACT123750.023.41404212-13
CATGGGG58200.023.1907654
CATGGGA68050.022.0766944
GTACTTT137450.021.4043114-15
ACGCAGA267350.021.3921977
AGTACTT136000.021.05752812-13
CGCAGAG270000.020.8957448
AGAGTAC204500.019.64153910-11
GCAGAGT264650.019.5767789
GTATAGG13950.018.418111
Produced by FastQC (version 0.11.5)